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Dicty_cDB
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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Contig-U05231-1
Contig ID Contig-U05231-1
Contig update 2001. 8.29
Contig sequence 
>Contig-U05231-1 (Contig-U05231-1Q) /CSM_Contig/Contig-U05231-1Q.Seq.d
AAAAGTTTAAAAAAAAATAAAAAATAAAAAAAGACCAAAGTTATAAATAT
ATTTAAAAATAAAAATAAAAACTTTATAAAAATATCTAAAGAAATAAAAA
AAAATAAAATAAAATGACATTCTTGAGTAAAAAACCTCAAATATTTAAAA
ATATTGAAAAAGATATAAATAAAATAAAATTTAAAAAATCAAATAAAATA
AAATCAGATTGTAATAATAGTAATAATAAAAATGGTTTGATAGAGTGTAT
ATCAACTTTAGAAGATGATAATCCATGGGATGTTTATACCAAAATAATGA
AAGAAGAAGGATTTTTAAAAAGTTGTATATTAGATTTAAATGATGGTGAA
GTTAATAGTAGTAGTGGTGCATCACCATTAAATGTTGCATACCAAGAATA
CCAAGAAATTAAAAATATTTTGGAAGGTAATAATTCAATTTATATAAATT
GTGACTCAACTAGATTCCAAATTCAANTGACATAATGATTACTGGTATAA
GTTCAAATTCATTATCATTCATATTACGAAGAACTGCAAATAAATGTATT
ATTGGAAATTTTCCAACCTTCAACAATTCTAGTCGACCATGCTCATTGTA
AAGTCTCTGATATTTATAATAAAATTACAAATTTTGATAAAAATGAAAGA
ATTACAAATTTAATTTTTTCATTCTCCGAAGATAGTATAAAAAATATTGT
AAATTAAAAAAAAAAA
Gap no gap
Contig length 716
Chromosome number (1..6, M) 3
Chromosome length 6358359
Start point 5577362
End point 5578085
Strand (PLUS/MINUS) PLUS
Number of clones 1
Number of EST 1
Link to clone list U05231
List of clone(s) 

est1=SSG420E,1,717
Translated Amino Acid sequence 
kfkkk*kikkdqsykyi*k*k*klykni*rnkkk*NKMTFLSKKPQIFKNIEKDINKIKF
KKSNKIKSDCNNSNNKNGLIECISTLEDDNPWDVYTKIMKEEGFLKSCILDLNDGEVNSS
SGASPLNVAYQEYQEIKNILEGNNSIYINCDSTRFQIQXT**llv*vqihyhsyyeelqi
nvlleifqpstilvdhahckvsdiynkitnfdkneritnlifsfsedsiknivn*kkk
Translated Amino Acid sequence (All Frames) 
Frame A:
kslkknkk*kktkvinifknknknfikiskeikknkik*hs*vknlkylkilkki*ik*n
lknqik*nqiviiviikmv**svyql*kmiihgmfipk**kkkdf*kvvy*i*mmvkliv
vvvhhh*mlhtkntkklkifwkviiqfi*ivtqldskfx*hndywykfkfiiihitknck
*myywkfsnlqqf*stmlivkslifiiklqilikmkelqi*ffhspkiv*kil*ikkk


Frame B:
kv*kkiknkkrpkl*iylkikiktl*kylkk*kkik*ndile*ktsni*ky*kryk*nki
*kik*nkirl******kwfdrvyinfrr**smgclyqnnerrrifkklyirfk*w*s***
*wcitikccipriprn*kyfgr**fnlykl*ln*ipnsxdimitgissnslsfilrrtan
kciignfptfnnssrpcsl*sl*yl**nykf**k*knykfnffilrr*ykkycklkkk


Frame C:
kfkkk*kikkdqsykyi*k*k*klykni*rnkkk*NKMTFLSKKPQIFKNIEKDINKIKF
KKSNKIKSDCNNSNNKNGLIECISTLEDDNPWDVYTKIMKEEGFLKSCILDLNDGEVNSS
SGASPLNVAYQEYQEIKNILEGNNSIYINCDSTRFQIQXT**llv*vqihyhsyyeelqi
nvlleifqpstilvdhahckvsdiynkitnfdkneritnlifsfsedsiknivn*kkk
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U05231-1 (Contig-U05231-1Q)
/CSM_Contig/Contig-U05231-1Q.Seq.d
         (716 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U05231-1 (Contig-U05231-1Q) /CSM_Contig/Conti...   930  0.0
Contig-U06231-1 (Contig-U06231-1Q) /CSM_Contig/Conti...    42  8e-04
Contig-U06002-1 (Contig-U06002-1Q) /CSM_Contig/Conti...    38  0.013
Contig-U13002-1 (Contig-U13002-1Q) /CSM_Contig/Conti...    36  0.050
Contig-U09658-1 (Contig-U09658-1Q) /CSM_Contig/Conti...    36  0.050
Contig-U09530-1 (Contig-U09530-1Q) /CSM_Contig/Conti...    36  0.050
Contig-U09480-1 (Contig-U09480-1Q) /CSM_Contig/Conti...    36  0.050
Contig-U02225-1 (Contig-U02225-1Q) /CSM_Contig/Conti...    36  0.050
Contig-U11682-1 (Contig-U11682-1Q) /CSM_Contig/Conti...    34  0.20
Contig-U10780-1 (Contig-U10780-1Q) /CSM_Contig/Conti...    34  0.20

>Contig-U05231-1 (Contig-U05231-1Q) /CSM_Contig/Contig-U05231-1Q.Seq.d
          Length = 716

 Score =  930 bits (469), Expect = 0.0
 Identities = 472/472 (100%)
 Strand = Plus / Plus

                                                                       
Query: 234 ggtttgatagagtgtatatcaactttagaagatgataatccatgggatgtttataccaaa 293
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 234 ggtttgatagagtgtatatcaactttagaagatgataatccatgggatgtttataccaaa 293

                                                                       
Query: 294 ataatgaaagaagaaggatttttaaaaagttgtatattagatttaaatgatggtgaagtt 353
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 294 ataatgaaagaagaaggatttttaaaaagttgtatattagatttaaatgatggtgaagtt 353

                                                                       
Query: 354 aatagtagtagtggtgcatcaccattaaatgttgcataccaagaataccaagaaattaaa 413
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 354 aatagtagtagtggtgcatcaccattaaatgttgcataccaagaataccaagaaattaaa 413

                                                                       
Query: 414 aatattttggaaggtaataattcaatttatataaattgtgactcaactagattccaaatt 473
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 414 aatattttggaaggtaataattcaatttatataaattgtgactcaactagattccaaatt 473

                                                                       
Query: 474 caantgacataatgattactggtataagttcaaattcattatcattcatattacgaagaa 533
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 474 caantgacataatgattactggtataagttcaaattcattatcattcatattacgaagaa 533

                                                                       
Query: 534 ctgcaaataaatgtattattggaaattttccaaccttcaacaattctagtcgaccatgct 593
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 534 ctgcaaataaatgtattattggaaattttccaaccttcaacaattctagtcgaccatgct 593

                                                                       
Query: 594 cattgtaaagtctctgatatttataataaaattacaaattttgataaaaatgaaagaatt 653
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 594 cattgtaaagtctctgatatttataataaaattacaaattttgataaaaatgaaagaatt 653

                                                               
Query: 654 acaaatttaattttttcattctccgaagatagtataaaaaatattgtaaatt 705
           ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 654 acaaatttaattttttcattctccgaagatagtataaaaaatattgtaaatt 705


 Score = 32.2 bits (16), Expect = 0.78
 Identities = 16/16 (100%)
 Strand = Plus / Minus

                           
Query: 436 caatttatataaattg 451
           ||||||||||||||||
Sbjct: 451 caatttatataaattg 436


>Contig-U06231-1 (Contig-U06231-1Q) /CSM_Contig/Contig-U06231-1Q.Seq.d
          Length = 552

 Score = 42.1 bits (21), Expect = 8e-04
 Identities = 27/29 (93%)
 Strand = Plus / Plus

                                        
Query: 393 caagaataccaagaaattaaaaatatttt 421
           |||||||  ||||||||||||||||||||
Sbjct: 189 caagaatggcaagaaattaaaaatatttt 217


 Score = 34.2 bits (17), Expect = 0.20
 Identities = 35/41 (85%)
 Strand = Plus / Plus

                                                    
Query: 325 gtatattagatttaaatgatggtgaagttaatagtagtagt 365
           |||| ||||||||| ||  ||||||| | ||||||||||||
Sbjct: 112 gtattttagatttagatagtggtgaattaaatagtagtagt 152


>Contig-U06002-1 (Contig-U06002-1Q) /CSM_Contig/Contig-U06002-1Q.Seq.d
          Length = 543

 Score = 38.2 bits (19), Expect = 0.013
 Identities = 22/23 (95%)
 Strand = Plus / Plus

                                  
Query: 651 attacaaatttaattttttcatt 673
           ||||||| |||||||||||||||
Sbjct: 467 attacaagtttaattttttcatt 489


 Score = 34.2 bits (17), Expect = 0.20
 Identities = 20/21 (95%)
 Strand = Plus / Minus

                                
Query: 620 taaaattacaaattttgataa 640
           ||||||||||||| |||||||
Sbjct: 208 taaaattacaaatattgataa 188


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 13,363
Number of Sequences: 6905
Number of extensions: 13363
Number of successful extensions: 1556
Number of sequences better than 10.0: 164
length of query: 716
length of database: 5,674,871
effective HSP length: 16
effective length of query: 700
effective length of database: 5,564,391
effective search space: 3895073700
effective search space used: 3895073700
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 7.15
Homology vs DNA 
Query= Contig-U05231-1 (Contig-U05231-1Q) /CSM_Contig/Contig-U05231-1Q.Seq.d
         (716 letters)

Database: ddbj_B
           105,743,758 sequences; 104,622,809,269 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C94111) Dictyostelium discoideum slug cDNA, clone SSG420.        167   2e-77  3
(AU261378) Dictyostelium discoideum vegetative cDNA clone:VS...    42   5e-04  2
(AC157209) Bos taurus clone CH240-46L12, WORKING DRAFT SEQUE...    54   0.008  1
(AC156191) Bos taurus clone CH240-48I4, WORKING DRAFT SEQUEN...    54   0.008  1
(AF228564) Mansonella ozzardi clone Mo9-3 18S ribosomal RNA ...    46   0.41   2
(AC121070) Sus scrofa clone RP44-299L10, WORKING DRAFT SEQUE...    48   0.48   1
(CU638827) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.48   1
(CP000885) Clostridium phytofermentans ISDg, complete genome.      48   0.48   1
(BH134395) ENTNE45TR Entamoeba histolytica Sheared DNA Entam...    38   0.51   3
(EJ201977) 1092344308854 Global-Ocean-Sampling_GS-27-01-01-1...    40   1.2    2
(AZ532406) ENTBY35TF Entamoeba histolytica Sheared DNA Entam...    38   1.7    3
(AC145021) Medicago truncatula clone mth2-23a16, complete se...    46   1.9    1
(FB917067) Sequence 136340 from Patent WO2008034648.               46   1.9    1
(AF228562) Mansonella ozzardi clone Mo8-4 18S ribosomal RNA ...    46   1.9    1
(AF228561) Mansonella ozzardi clone Mo8-3 18S ribosomal RNA ...    46   1.9    1
(AF228559) Mansonella ozzardi clone Mo8-1 18S ribosomal RNA ...    46   1.9    1
(AC230559) Bos taurus clone CH240-505E5, WORKING DRAFT SEQUE...    46   1.9    1
(EJ555871) 1092959498692 Global-Ocean-Sampling_GS-29-01-01-1...    46   1.9    1
(EJ553476) 1092959475640 Global-Ocean-Sampling_GS-29-01-01-1...    46   1.9    1
(CR335975) mte1-66J10FM1 BAC end, cultivar Jemalong A17 of M...    46   1.9    1
(CP000721) Clostridium beijerinckii NCIMB 8052, complete gen...    46   1.9    1
(BH165394) ENTSP11TR Entamoeba histolytica Sheared DNA Entam...    38   1.9    3
(AU061802) Dictyostelium discoideum slug cDNA, clone SLG149.       42   2.6    2
(AC125508) Papio anubis clone RP41-310M7, WORKING DRAFT SEQU...    40   2.6    5
(DQ384437) Homo sapiens clone fa0555 genomic sequence.             34   3.1    3
(FP015797) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    34   3.1    6
(EJ753537) 1092963054889 Global-Ocean-Sampling_GS-30-02-01-1...    42   3.5    2
(AC140276) Mus musculus BAC clone RP23-336J13 from 8, comple...    42   3.5    2
(FI408109) CH322-134F09.y CHORI-322 Drosophila melanogaster ...    36   3.6    2
(BV499668) S222P6581FH7.T0 Karlien Pan troglodytes verus STS...    36   3.6    3
(EJ547854) 1092959415692 Global-Ocean-Sampling_GS-29-01-01-1...    42   4.1    2
(AM285309) Spiroplasma citri GII3-3X chromosome, contig Cont...    36   4.2    3
(EJ740962) 1092962017680 Global-Ocean-Sampling_GS-30-02-01-1...    42   4.5    2
(EU573699) Ucides cordatus isolate CARANPR953 D-loop, partia...    30   4.5    3
(ER527147) 1093015696130 Global-Ocean-Sampling_GS-35-01-01-1...    42   4.7    2
(DU884823) 399968 Tomato HindIII BAC Library Solanum lycoper...    34   4.8    2
(BA000026) Mycoplasma penetrans HF-2 DNA, complete genome.         40   4.9    12
(CP000849) Rickettsia bellii OSU 85-389, complete genome.          34   5.4    10
(AL732370) Mouse DNA sequence from clone RP23-413I21 on chro...    44   7.5    1
(AC152982) Mus musculus 10 BAC RP23-36F22 (Roswell Park Canc...    44   7.5    1
(AP008215) Oryza sativa (japonica cultivar-group) genomic DN...    44   7.5    1
(AP005570) Oryza sativa Japonica Group genomic DNA, chromoso...    44   7.5    1
(AP005424) Oryza sativa Japonica Group genomic DNA, chromoso...    44   7.5    1
(EA689306) Sequence 68185 from patent US 7365185.                  44   7.5    1
(EA654972) Sequence 33851 from patent US 7365185.                  44   7.5    1
(U77412) Caenorhabditis elegans O-linked GlcNAc transferase ...    44   7.5    1
(U21320) Caenorhabditis elegans cosmid K04G7, complete seque...    44   7.5    1
(AF228563) Mansonella ozzardi clone Mo9-1 18S ribosomal RNA ...    44   7.5    1
(AF228560) Mansonella ozzardi clone Mo8-2 18S ribosomal RNA ...    44   7.5    1
(BX088563) Human DNA sequence from clone RP11-1066D24 on chr...    44   7.5    1
(AC160489) Bos taurus clone CH240-92K19, WORKING DRAFT SEQUE...    44   7.5    1
(AC134639) Rattus norvegicus clone CH230-416N3, *** SEQUENCI...    44   7.5    1
(AC128823) Rattus norvegicus clone CH230-492P4, *** SEQUENCI...    44   7.5    1
(AC120062) Rattus norvegicus clone CH230-180H10, WORKING DRA...    44   7.5    1
(AC109888) Rattus norvegicus clone CH230-312P6, *** SEQUENCI...    44   7.5    1
(AC109380) Rattus norvegicus clone CH230-60A18, *** SEQUENCI...    44   7.5    1
(DQ273272) Allium cepa clone S1D12, *** SEQUENCING IN PROGRE...    44   7.5    1
(CU928139) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   7.5    1
(CU571288) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   7.5    1
(AL954647) Mouse DNA sequence *** SEQUENCING CANCELLED *** f...    44   7.5    1
(AC202254) Colobus guereza clone CH272-4F21, WORKING DRAFT S...    44   7.5    1
(AC201109) Strongylocentrotus purpuratus clone R3-13K22, WOR...    44   7.5    1
(AC165604) Bos taurus clone CH240-169N2, WORKING DRAFT SEQUE...    44   7.5    1
(FI032020) CHO_OF6946xb08f1.ab1 CHO_OF6 Nicotiana tabacum ge...    44   7.5    1
(ET733545) CHO_OF170xk10r1.ab1 CHO_OF Nicotiana tabacum geno...    44   7.5    1
(EK381157) 1095469462202 Global-Ocean-Sampling_GS-31-01-01-1...    44   7.5    1
(DX014950) KBrB006K06R KBrB, Brassica rapa BamHI BAC library...    44   7.5    1
(DH819112) Rattus norvegicus DNA, BAC clone: RNB1-422F07, 5'...    44   7.5    1
(AG316072) Mus musculus molossinus DNA, clone:MSMg01-100F14....    44   7.5    1
(AU222145) Caenorhabditis elegans cDNA clone:yk1012a11 : 3' ...    44   7.5    1
(AU219212) Caenorhabditis elegans cDNA clone:yk874e09 : 3' e...    44   7.5    1
(CO805375) Pcl11-004-H12-A-096.b Subtracted cDNA library fro...    44   7.5    1
(C12767) Caenorhabditis elegans cDNA clone yk155g3 : 5' end,...    44   7.5    1
(BJ812481) Caenorhabditis elegans cDNA clone:yk1468c01 : 3' ...    44   7.5    1
(BJ789475) Caenorhabditis elegans cDNA clone:yk1618e06 : 3' ...    44   7.5    1
(BJ785263) Caenorhabditis elegans cDNA clone:yk1568f06 : 3' ...    44   7.5    1
(BJ155789) Caenorhabditis elegans cDNA clone:yk1346c06 : 3' ...    44   7.5    1
(BJ153342) Caenorhabditis elegans cDNA clone:yk1318c08 : 3' ...    44   7.5    1
(BJ151366) Caenorhabditis elegans cDNA clone:yk1294f10 : 3' ...    44   7.5    1
(BJ151145) Caenorhabditis elegans cDNA clone:yk1292b08 : 3' ...    44   7.5    1
(BJ146449) Caenorhabditis elegans cDNA clone:yk1233f07 : 3' ...    44   7.5    1
(BJ145756) Caenorhabditis elegans cDNA clone:yk1222h07 : 3' ...    44   7.5    1
(BJ142756) Caenorhabditis elegans cDNA clone:yk1188b08 : 3' ...    44   7.5    1
(BJ142736) Caenorhabditis elegans cDNA clone:yk1187h11 : 3' ...    44   7.5    1
(BJ136310) Caenorhabditis elegans cDNA clone:yk1111d12 : 3' ...    44   7.5    1
(BJ134189) Caenorhabditis elegans cDNA clone:yk1085f02 : 3' ...    44   7.5    1
(BJ132560) Caenorhabditis elegans cDNA clone:yk1067d06 : 3' ...    44   7.5    1
(BJ131737) Caenorhabditis elegans cDNA clone:yk1058b07 : 3' ...    44   7.5    1
(BJ108744) Caenorhabditis elegans cDNA clone:yk1111d12 : 5' ...    44   7.5    1
(BF092600) MR4-TN0110-080900-201-b10 TN0110 Homo sapiens cDN...    44   7.5    1
(AC073273) Homo sapiens BAC clone RP11-643A21 from 7, comple...    34   8.2    4
(AC144644) Medicago truncatula clone mth2-14l3, complete seq...    34   9.8    6

>(C94111) Dictyostelium discoideum slug cDNA, clone SSG420.
          Length = 253

 Score =  167 bits (84), Expect(3) = 2e-77
 Identities = 88/90 (97%)
 Strand = Plus / Plus


Query: 468 caaattcaantgacataatgattactggtataagttcaaattcattatcattcatattac 527
           ||||||||| ||||||||||||||||||||||| ||||||||||||||||||||||||||
Sbjct: 1   caaattcaactgacataatgattactggtataanttcaaattcattatcattcatattac 60


Query: 528 gaagaactgcaaataaatgtattattggaa 557
           ||||||||||||||||||||||||||||||
Sbjct: 61  gaagaactgcaaataaatgtattattggaa 90

 Score =  109 bits (55), Expect(3) = 2e-77
 Identities = 55/55 (100%)
 Strand = Plus / Plus


Query: 557 aattttccaaccttcaacaattctagtcgaccatgctcattgtaaagtctctgat 611
           |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 89  aattttccaaccttcaacaattctagtcgaccatgctcattgtaaagtctctgat 143

 Score = 71.9 bits (36), Expect(3) = 2e-77
 Identities = 36/36 (100%)
 Strand = Plus / Plus


Query: 670 cattctccgaagatagtataaaaaatattgtaaatt 705
           ||||||||||||||||||||||||||||||||||||
Sbjct: 202 cattctccgaagatagtataaaaaatattgtaaatt 237

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 105743758
Number of Hits to DB: 629,673,684
Number of extensions: 43078315
Number of successful extensions: 3822736
Number of sequences better than 10.0: 95
Length of query: 716
Length of database: 104,622,809,269
Length adjustment: 24
Effective length of query: 692
Effective length of database: 102,084,959,077
Effective search space: 70642791681284
Effective search space used: 70642791681284
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 8. 1
Homology vs Protein 
Query= Contig-U05231-1 (Contig-U05231-1Q) /CSM_Contig/Contig-U05231-1Q.Seq.d
         (716 letters)

Database: nrp_A
           3,268,448 sequences; 1,061,185,681 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116100_17(AC116100|pid:none) Dictyostelium discoideum chromoso...    45   0.002
CR382134_505(CR382134|pid:none) Debaryomyces hansenii strain CBS...    35   1.8
CR626927_3608(CR626927|pid:none) Bacteroides fragilis NCTC 9343,...    33   6.8
AP006841_3929(AP006841|pid:none) Bacteroides fragilis YCH46 DNA,...    33   6.8
AL844505_40(AL844505|pid:none) Plasmodium falciparum 3D7 chromos...    33   8.8
AE016795_1892(AE016795|pid:none) Vibrio vulnificus CMCP6 chromos...    33   8.8

>AC116100_17(AC116100|pid:none) Dictyostelium discoideum chromosome
           2 map 6163571-6199719 strain AX4, complete sequence.
          Length = 155

 Score = 45.4 bits (106), Expect = 0.002
 Identities = 37/139 (26%), Positives = 63/139 (45%), Gaps = 12/139 (8%)
 Frame = +3

Query: 285 YTKIMKEEGFLKSCILDLNDGEVNSSSGASPLNVAYQEYQEIKNILEGNNSIYINCDSTR 464
           Y K + + GF K  I+D++ G++   S     NV Y EYQEI N+  G++SI +  +  R
Sbjct: 17  YIKKLIDNGFFKCGIIDIDSGDMICIS--EEFNVCYVEYQEILNLFNGSSSISLLINGDR 74

Query: 465 FQIQXT**LLV*VQIHYHSYYEELQINVLLEIFQPST------------ILVDHAHCKVS 608
           + +  +  + +  +    S      IN L  +    T               + A   +
Sbjct: 75  YYVNTSDDIRIQGKTTLKSSGNTSTINGLQSLIIRRTKGKCVIGVYKENQTKELAEKYIE 134

Query: 609 DIYNKITNFDKNERITNLI 665
           +I + IT  ++NE I+NLI
Sbjct: 135 EIIDDITKLNENENISNLI 153

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 711,844,433
Number of extensions: 12597975
Number of successful extensions: 36179
Number of sequences better than 10.0: 6
Number of HSP's gapped: 36154
Number of HSP's successfully gapped: 8
Length of query: 238
Length of database: 1,061,185,681
Length adjustment: 125
Effective length of query: 113
Effective length of database: 652,629,681
Effective search space: 73747153953
Effective search space used: 73747153953
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 31 (16.5 bits)
PSORT 

 psg: 0.59  gvh: 0.24  alm: 0.55  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 24.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U05231-1 is nuc
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 1
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0