NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U05086-1
Contig ID Contig-U05086-1
Contig update 2001. 8.29
Contig sequence 
>Contig-U05086-1 (Contig-U05086-1Q) /CSM_Contig/Contig-U05086-1Q.Seq.d
AAAATTTTCTTTATTTTTTAGTTTTGTTTTTTTTTTTTTTTTATATAAAT
ATCAAACCATATAAAAAGTTTTTTATTTGAAATTAAAAAAAAAAAAAAAA
AAAAAAAAGACAAAATCACAAACTAAAATATCAAAATAACCCACTATAAA
AATAATATATATATAACACCTTAAAAAATGAAACTCTTTGGTAAACCAAA
ACCAAAACAAGATACTCAAGAAACAATTGGTAAACTTAGATTAACATTAG
ATATGCTTGAAAAAAGGCAAACATTTTTACAAGATAAATTAGAAAAAGAG
AACAAGAAAAAGCAAAGGTCTTTAGTACAGCCCAAAAAAAGAAAGAGAGA
CGCATTTATTATGTTTAAAAAAGAGCAATTAATTTTCAAAATGAGGTTAC
AAAATTACCAAGGTTCATATGATACATTAAACTCAACAAATTTTTGCATT
AGAGAATGCAAAGATGAAATATGGCAATTATGAAATTCCAATGAGAGATG
GTGCAAGATCCATTAAGAGAATTCCCATGGTCACCTTACTATTATCGATA
AAGTTGATGACGTAAATTGAAAAGATTCAAGAGCAAATGGAAATTCACGA
AGAAATTTCAAATGCTATCAGTCAACCATTAGGTAACCAAGTTGAAGATG
AAGAAGAATTACTAAGAGAATTAGCTGAATATGAGCAAGAAGAATTAGAT
GCTCAATTACTTAATATTAAAGCACCTTCAAGAGAATTACCACAAGAAAT
TCAAGTAAATTTCCCAACTATTTCAAAAACTGTACCAAAACTTTCAAAAG
AAGAAGAAGAGATTAGAGCTTTGGAAGAATCTTTAGCAATGTAAATTAAT
TTCAATAAAAAATAGAAAAGAAAAAAAAAACAAAAAAAAAAAGGAATACA
AAAAAAAAAAAAAAAAACNAAAAACAAAACTTAAACCAACCACACTGTTT
TAATTTTATTTATTTTAAATTTAATAAAACTAAAAAAAAATATCTCTTTT
TTTTTTTTTTTAATAAATATATAAA
Gap no gap
Contig length 1025
Chromosome number (1..6, M) 2
Chromosome length 8467578
Start point 5497914
End point 5498940
Strand (PLUS/MINUS) PLUS
Number of clones 6
Number of EST 6
Link to clone list U05086
List of clone(s) 

est1=SSK154E,1,890
est2=SSI196E,31,856
est3=SSJ340Z,107,913
est4=SSK218Z,220,901
est5=SSJ342Z,253,899
est6=SSF178E,437,1026
Translated Amino Acid sequence 
kiffif*fcfffffi*isnhiksflfeikkkkkkkkdkitn*nikithyknniyitp*KM
KLFGKPKPKQDTQETIGKLRLTLDMLEKRQTFLQDKLEKENKKKQRSLVQPKKRKRDAFI
MFKKEQLIFKMRLQNYQGSYDTLNSTNFCIRECKDEIWQL*nsnerwckih*enshghlt
iidkvddvn*kdsrangnsrrnfkcyqstir*ps*r*rritkris*i*arrircsit*y*
stfkrittrnsskfpnyfknctktfkrrrrd*sfgrifsnvn*fq*kiekkkktkkkrnt
kkkkknxkqnlnqphcfnfiyfkfnktkkkylfffffnkyi
Translated Amino Acid sequence (All Frames) 
Frame A:
kiffif*fcfffffi*isnhiksflfeikkkkkkkkdkitn*nikithyknniyitp*KM
KLFGKPKPKQDTQETIGKLRLTLDMLEKRQTFLQDKLEKENKKKQRSLVQPKKRKRDAFI
MFKKEQLIFKMRLQNYQGSYDTLNSTNFCIRECKDEIWQL*nsnerwckih*enshghlt
iidkvddvn*kdsrangnsrrnfkcyqstir*ps*r*rritkris*i*arrircsit*y*
stfkrittrnsskfpnyfknctktfkrrrrd*sfgrifsnvn*fq*kiekkkktkkkrnt
kkkkknxkqnlnqphcfnfiyfkfnktkkkylfffffnkyi


Frame B:
kfslffsfvfffflykyqti*kvfylklkkkkkkkktksqtkisk*ptikiiyi*hlkk*
nslvnqnqnkilkkqlvnld*h*iclkkgkhfykin*kkrtrkskgl*yspkkerethll
clkksn*fsk*gykitkvhmih*tqqifalenakmkygnyeipmrdgarsikripmvtll
lsiklmt*iekiqeqmeiheeisnaisqplgnqvedeeellrelaeyeqeeldaqllnik
apsrelpqeiqvnfptisktvpklskeeeeiraleeslam*infnkk*krkkkqkkkgiq
kkkkktknkt*tnhtvlilfilnliklkknisfffflini*


Frame C:
nflyflvlfffffyinikpykkffi*n*kkkkkkkrqnhklkyqnnpl*k*yiyntlkne
tlw*tktktrysrnnw*t*inirya*kkaniftr*irkreqekakvfstaqkkkerriyy
v*krainfqnevtklprfi*yiklnkflh*rmqr*nmaimkfq*emvqdplrefpwspyy
yr*s**rklkrfkskwkftkkfqmlsvnh*vtklkmkkny*en*lnmskkn*mlnylilk
hlqenyhkkfk*isqlfqklyqnfqkkkkrlelwknl*qcklisiknrkekknkkkkeyk
kkkkkxktklkpttlf*fylf*i**n*kkislffff**iyk
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U05086-1 (Contig-U05086-1Q)
/CSM_Contig/Contig-U05086-1Q.Seq.d
         (1025 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U05086-1 (Contig-U05086-1Q) /CSM_Contig/Conti...  1328  0.0
Contig-U08583-1 (Contig-U08583-1Q) /CSM_Contig/Conti...    48  2e-05
Contig-U14766-1 (Contig-U14766-1Q) /CSM_Contig/Conti...    46  8e-05
Contig-U14292-1 (Contig-U14292-1Q) /CSM_Contig/Conti...    40  0.005
Contig-U14187-1 (Contig-U14187-1Q) /CSM_Contig/Conti...    40  0.005
Contig-U10739-1 (Contig-U10739-1Q) /CSM_Contig/Conti...    40  0.005
Contig-U04354-1 (Contig-U04354-1Q) /CSM_Contig/Conti...    40  0.005
Contig-U14053-1 (Contig-U14053-1Q) /CSM_Contig/Conti...    38  0.018
Contig-U13887-1 (Contig-U13887-1Q) /CSM_Contig/Conti...    38  0.018

>Contig-U05086-1 (Contig-U05086-1Q) /CSM_Contig/Contig-U05086-1Q.Seq.d
          Length = 1025

 Score = 1328 bits (670), Expect = 0.0
 Identities = 684/691 (98%)
 Strand = Plus / Plus

                                                                       
Query: 166 acaccttaaaaaatgaaactctttggtaaaccaaaaccaaaacaagatactcaagaaaca 225
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 166 acaccttaaaaaatgaaactctttggtaaaccaaaaccaaaacaagatactcaagaaaca 225

                                                                       
Query: 226 attggtaaacttagattaacattagatatgcttgaaaaaaggcaaacatttttacaagat 285
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 226 attggtaaacttagattaacattagatatgcttgaaaaaaggcaaacatttttacaagat 285

                                                                       
Query: 286 aaattagaaaaagagaacaagaaaaagcaaaggtctttagtacagcccnnnnnnngaaag 345
           ||||||||||||||||||||||||||||||||||||||||||||||||       |||||
Sbjct: 286 aaattagaaaaagagaacaagaaaaagcaaaggtctttagtacagcccaaaaaaagaaag 345

                                                                       
Query: 346 agagacgcatttattatgtttaaaaaagagcaattaattttcaaaatgaggttacaaaat 405
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 346 agagacgcatttattatgtttaaaaaagagcaattaattttcaaaatgaggttacaaaat 405

                                                                       
Query: 406 taccaaggttcatatgatacattaaactcaacaaatttttgcattagagaatgcaaagat 465
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 406 taccaaggttcatatgatacattaaactcaacaaatttttgcattagagaatgcaaagat 465

                                                                       
Query: 466 gaaatatggcaattatgaaattccaatgagagatggtgcaagatccattaagagaattcc 525
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 466 gaaatatggcaattatgaaattccaatgagagatggtgcaagatccattaagagaattcc 525

                                                                       
Query: 526 catggtcaccttactattatcgataaagttgatgacgtaaattgaaaagattcaagagca 585
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 526 catggtcaccttactattatcgataaagttgatgacgtaaattgaaaagattcaagagca 585

                                                                       
Query: 586 aatggaaattcacgaagaaatttcaaatgctatcagtcaaccattaggtaaccaagttga 645
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 586 aatggaaattcacgaagaaatttcaaatgctatcagtcaaccattaggtaaccaagttga 645

                                                                       
Query: 646 agatgaagaagaattactaagagaattagctgaatatgagcaagaagaattagatgctca 705
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 646 agatgaagaagaattactaagagaattagctgaatatgagcaagaagaattagatgctca 705

                                                                       
Query: 706 attacttaatattaaagcaccttcaagagaattaccacaagaaattcaagtaaatttccc 765
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 706 attacttaatattaaagcaccttcaagagaattaccacaagaaattcaagtaaatttccc 765

                                                                       
Query: 766 aactatttcaaaaactgtaccaaaactttcaaaagaagaagaagagattagagctttgga 825
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 766 aactatttcaaaaactgtaccaaaactttcaaaagaagaagaagagattagagctttgga 825

                                          
Query: 826 agaatctttagcaatgtaaattaatttcaat 856
           |||||||||||||||||||||||||||||||
Sbjct: 826 agaatctttagcaatgtaaattaatttcaat 856


 Score =  133 bits (67), Expect = 4e-31
 Identities = 67/67 (100%)
 Strand = Plus / Plus

                                                                       
Query: 930 cttaaaccaaccacactgttttaattttatttattttaaatttaataaaactaaaaaaaa 989
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 930 cttaaaccaaccacactgttttaattttatttattttaaatttaataaaactaaaaaaaa 989

                  
Query: 990 atatctc 996
           |||||||
Sbjct: 990 atatctc 996


 Score = 83.8 bits (42), Expect = 3e-16
 Identities = 42/42 (100%)
 Strand = Plus / Plus

                                                    
Query: 43 atataaatatcaaaccatataaaaagttttttatttgaaatt 84
          ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 43 atataaatatcaaaccatataaaaagttttttatttgaaatt 84


>Contig-U08583-1 (Contig-U08583-1Q) /CSM_Contig/Contig-U08583-1Q.Seq.d
          Length = 411

 Score = 48.1 bits (24), Expect = 2e-05
 Identities = 24/24 (100%)
 Strand = Plus / Plus

                                   
Query: 948 ttttaattttatttattttaaatt 971
           ||||||||||||||||||||||||
Sbjct: 94  ttttaattttatttattttaaatt 117


>Contig-U14766-1 (Contig-U14766-1Q) /CSM_Contig/Contig-U14766-1Q.Seq.d
          Length = 323

 Score = 46.1 bits (23), Expect = 8e-05
 Identities = 26/27 (96%)
 Strand = Plus / Minus

                                      
Query: 955 tttatttattttaaatttaataaaact 981
           ||||||||||||||||||||| |||||
Sbjct: 287 tttatttattttaaatttaattaaact 261


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 24,151
Number of Sequences: 6905
Number of extensions: 24151
Number of successful extensions: 3982
Number of sequences better than 10.0: 568
length of query: 1025
length of database: 5,674,871
effective HSP length: 16
effective length of query: 1009
effective length of database: 5,564,391
effective search space: 5614470519
effective search space used: 5614470519
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 7.11
Homology vs DNA 
Query= Contig-U05086-1 (Contig-U05086-1Q) /CSM_Contig/Contig-U05086-1Q.Seq.d
         (1025 letters)

Database: ddbj_B
           105,743,758 sequences; 104,622,809,269 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C90947) Dictyostelium discoideum slug cDNA, clone SSJ342.        622   0.0    3
(C91296) Dictyostelium discoideum slug cDNA, clone SSK218.        571   0.0    10
(AC116960) Dictyostelium discoideum chromosome 2 map complem...   383   0.0    14
(C91387) Dictyostelium discoideum slug cDNA, clone SSK154.        571   0.0    8
(C90946) Dictyostelium discoideum slug cDNA, clone SSJ340.        571   0.0    8
(C92740) Dictyostelium discoideum slug cDNA, clone SSF178.        571   0.0    8
(AU073737) Dictyostelium discoideum slug cDNA, clone SSI196.      476   e-130  1
(C90185) Dictyostelium discoideum slug cDNA, clone SSI196.        100   6e-58  6
(AU074255) Dictyostelium discoideum slug cDNA, clone SSK154.       84   7e-22  2
(EF506945) Leptosira terrestris UTEX 333 chloroplast, comple...    40   0.61   5
(AY084347) Arabidopsis thaliana clone 105072 mRNA, complete ...    48   0.69   1
(AK317010) Arabidopsis thaliana AT2G17500 mRNA, partial cds,...    48   0.69   1
(AK226321) Arabidopsis thaliana mRNA for hypothetical protei...    48   0.69   1
(AC007584) Arabidopsis thaliana chromosome 2 clone MJB20 map...    48   0.69   1
(AX652467) Sequence 2337 from Patent WO03000898.                   48   0.69   1
(AX509667) Sequence 4362 from Patent WO0216655.                    48   0.69   1
(AX461097) Sequence 26 from Patent WO0198480.                      48   0.69   1
(AC118470) Homo sapiens chromosome 1 clone RP11-634B7, compl...    48   0.69   1
(AC026038) Homo sapiens chromosome 1 clone RP11-109C14, WORK...    48   0.69   1
(BX820270) Arabidopsis thaliana Full-length cDNA Complete se...    48   0.69   1
(BX820250) Arabidopsis thaliana Full-length cDNA Complete se...    48   0.69   1
(BX819980) Arabidopsis thaliana Full-length cDNA Complete se...    48   0.69   1
(BX818906) Arabidopsis thaliana Full-length cDNA Complete se...    48   0.69   1
(ES387276) C5s130 C. finmarchicus C5 subtractive library, sh...    48   0.69   1
(EG909008) EST_ssal_evf_53911 ssalevf mixed_tissue Salmo sal...    48   0.69   1
(EG488315) AYBKJ31TF pooled cDNA populations Arabidopsis tha...    48   0.69   1
(EC760675) PSE00007706 rw_mgpallid Polysphondylium pallidum ...    48   0.69   1
(AV551320) Arabidopsis thaliana cDNA clone:RZ125b10R, 5' end.      48   0.69   1
(DR354037) 11060170 CERES-AN65 Arabidopsis thaliana cDNA clo...    48   0.69   1
(DR296716) 137082 CERES-148 Arabidopsis thaliana cDNA clone ...    48   0.69   1
(DR296715) 12841384 CERES-AN65 Arabidopsis thaliana cDNA clo...    48   0.69   1
(BP815657) Arabidopsis thaliana cDNA clone:RAFL22-39-I01, 5'...    48   0.69   1
(BP814511) Arabidopsis thaliana cDNA clone:RAFL22-35-P03, 5'...    48   0.69   1
(BP814148) Arabidopsis thaliana cDNA clone:RAFL22-34-M05, 5'...    48   0.69   1
(BP810035) Arabidopsis thaliana cDNA clone:RAFL24-24-G05, 5'...    48   0.69   1
(BP806287) Arabidopsis thaliana cDNA clone:RAFL24-09-E13, 5'...    48   0.69   1
(BP805445) Arabidopsis thaliana cDNA clone:RAFL24-06-A02, 5'...    48   0.69   1
(BP805175) Arabidopsis thaliana cDNA clone:RAFL24-04-N19, 5'...    48   0.69   1
(BP804884) Arabidopsis thaliana cDNA clone:RAFL24-03-K07, 5'...    48   0.69   1
(BP803659) Arabidopsis thaliana cDNA clone:RAFL23-30-F14, 5'...    48   0.69   1
(BP800212) Arabidopsis thaliana cDNA clone:RAFL23-14-F17, 5'...    48   0.69   1
(T04264) 311 Lambda-PRL2 Arabidopsis thaliana cDNA clone 34G...    48   0.69   1
(AV822951) Arabidopsis thaliana cDNA clone:RAFL05-13-I20, 5'...    48   0.69   1
(AC166824) Mus musculus chromosome 6 clone RP23-385C17, *** ...    36   2.2    6
(AC147930) Ovis aries clone CH243-366O21, complete sequence.       46   2.7    1
(AL357150) Human DNA sequence from clone RP11-122C9 on chrom...    46   2.7    1
(AC013290) Homo sapiens, clone RP11-115P21, complete sequence.     46   2.7    1
(AC090410) Homo sapiens chromosome 18 clone RP11-879F4 map 1...    46   2.7    1
(AC069131) Homo sapiens chromosome 18 clone RP11-502P14, WOR...    46   2.7    1
(CU467085) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   2.7    1
(AP002895) Homo sapiens genomic DNA, chromosome 18q12, clone...    46   2.7    1
(AP001571) Homo sapiens genomic DNA, chromosome 18q12 clone:...    46   2.7    1
(AC215616) Nomascus leucogenys chromosome UNK clone CH271-89...    46   2.7    1
(AC195962) Zea mays chromosome 4 clone CH201-288O10; ZMMBBc0...    46   2.7    1
(AC025706) Homo sapiens chromosome 1 clone RP11-122C9, WORKI...    46   2.7    1
(AC025489) Homo sapiens chromosome 1 clone RP11-168A23 map 1...    46   2.7    1
(AC015672) Homo sapiens chromosome 18 clone RP11-70H1 map 18...    46   2.7    1
(ET986731) CHO_OF220xa19r1.ab1 CHO_OF Nicotiana tabacum geno...    46   2.7    1
(EJ314972) 1095403271526 Global-Ocean-Sampling_GS-27-01-01-1...    46   2.7    1
(DX825886) ZMMBLb0003N01.f ZMMBLb Zea mays genomic clone ZMM...    46   2.7    1
(DT763793) EST1197642 Aquilegia cDNA library Aquilegia formo...    46   2.7    1
(DT763792) EST1197641 Aquilegia cDNA library Aquilegia formo...    46   2.7    1
(CV159109) HISTERID5POLYT_C11_1_082 Hister sp. cDNA Hister s...    46   2.7    1
(CK753079) wmi01-6ms1-e12 Wmi01 Welwitschia mirabilis cDNA c...    46   2.7    1
(BX783385) Xenopus tropicalis EST, clone TEgg037n19 3'.            46   2.7    1
(BP830671) Arabidopsis thaliana cDNA clone:RAFL22-86-C20, 5'...    46   2.7    1
(BP187963) Dugesia japonica cDNA, clone: 03287_HH, expressed...    46   2.7    1
(AW191605) HIGB15.03.00 human islet cDNA differential displa...    46   2.7    1
(EJ082744) 1095460062934 Global-Ocean-Sampling_GS-26-01-01-1...    42   2.8    2
(ER939428) AF__Bb0039A21r AF__Bb Aquilegia formosa genomic, ...    40   3.4    2
(BA000016) Clostridium perfringens str. 13 DNA, complete gen...    36   3.6    17
(CE646066) tigr-gss-dog-17000367188236 Dog Library Canis lup...    40   3.6    2
(CR160273) Reverse strand read from insert in 5'HPRT inserti...    34   3.8    3
(AC154853) Mus musculus BAC clone RP23-21C15 from 17, comple...    42   3.9    5
(AR376812) Sequence 1818 from patent US 6605709.                   42   4.1    2
(CK751246) ltu01-7ms2-c12 Ltu01 Liriodendron tulipifera cDNA...    38   4.8    2
(AC119154) Rattus norvegicus strain Brown Norway clone RP31-...    40   6.5    2
(CP000246) Clostridium perfringens ATCC 13124, complete genome.    36   6.8    16
(EY623352) CAWW17252.fwd CAWW Capitella sp. I ECS-2004 Subtr...    34   6.9    2
(FS246155) Glycyrrhiza uralensis cDNA, clone: KAN066-I18.F, ...    30   7.2    3
(BP163377) Sus scrofa mRNA, clone:THY010119D03, 5' end, expr...    30   7.6    3
(AC109141) Mus musculus clone RP23-159E16, LOW-PASS SEQUENCE...    34   7.7    6
(AC117598) Mus musculus chromosome 1, clone RP23-398H16, com...    40   8.4    3
(AZ690475) ENTLF37TR Entamoeba histolytica Sheared DNA Entam...    34   9.4    2
(CR032476) Forward strand read from insert in 3'HPRT inserti...    30   9.5    3

>(C90947) Dictyostelium discoideum slug cDNA, clone SSJ342.
          Length = 648

 Score =  622 bits (314), Expect(3) = 0.0
 Identities = 314/314 (100%)
 Strand = Plus / Plus


Query: 543 tatcgataaagttgatgacgtaaattgaaaagattcaagagcaaatggaaattcacgaag 602
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 293 tatcgataaagttgatgacgtaaattgaaaagattcaagagcaaatggaaattcacgaag 352


Query: 603 aaatttcaaatgctatcagtcaaccattaggtaaccaagttgaagatgaagaagaattac 662
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 353 aaatttcaaatgctatcagtcaaccattaggtaaccaagttgaagatgaagaagaattac 412


Query: 663 taagagaattagctgaatatgagcaagaagaattagatgctcaattacttaatattaaag 722
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 413 taagagaattagctgaatatgagcaagaagaattagatgctcaattacttaatattaaag 472


Query: 723 caccttcaagagaattaccacaagaaattcaagtaaatttcccaactatttcaaaaactg 782
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 473 caccttcaagagaattaccacaagaaattcaagtaaatttcccaactatttcaaaaactg 532


Query: 783 taccaaaactttcaaaagaagaagaagagattagagctttggaagaatctttagcaatgt 842
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 533 taccaaaactttcaaaagaagaagaagagattagagctttggaagaatctttagcaatgt 592


Query: 843 aaattaatttcaat 856
           ||||||||||||||
Sbjct: 593 aaattaatttcaat 606

 Score =  400 bits (202), Expect(3) = 0.0
 Identities = 202/202 (100%)
 Strand = Plus / Plus


Query: 341 gaaagagagacgcatttattatgtttaaaaaagagcaattaattttcaaaatgaggttac 400
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 94  gaaagagagacgcatttattatgtttaaaaaagagcaattaattttcaaaatgaggttac 153


Query: 401 aaaattaccaaggttcatatgatacattaaactcaacaaatttttgcattagagaatgca 460
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 154 aaaattaccaaggttcatatgatacattaaactcaacaaatttttgcattagagaatgca 213


Query: 461 aagatgaaatatggcaattatgaaattccaatgagagatggtgcaagatccattaagaga 520
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 214 aagatgaaatatggcaattatgaaattccaatgagagatggtgcaagatccattaagaga 273


Query: 521 attcccatggtcaccttactat 542
           ||||||||||||||||||||||
Sbjct: 274 attcccatggtcaccttactat 295

 Score = 32.2 bits (16), Expect(3) = 0.0
 Identities = 16/16 (100%)
 Strand = Plus / Plus


Query: 318 gtctttagtacagccc 333
           ||||||||||||||||
Sbjct: 71  gtctttagtacagccc 86

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 105743758
Number of Hits to DB: 972,451,695
Number of extensions: 66005949
Number of successful extensions: 7658155
Number of sequences better than 10.0: 86
Length of query: 1025
Length of database: 104,622,809,269
Length adjustment: 24
Effective length of query: 1001
Effective length of database: 102,084,959,077
Effective search space: 102187044036077
Effective search space used: 102187044036077
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.31
Homology vs Protein 
Query= Contig-U05086-1 (Contig-U05086-1Q) /CSM_Contig/Contig-U05086-1Q.Seq.d
         (1025 letters)

Database: nrp_A
           3,268,448 sequences; 1,061,185,681 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86H98) RecName: Full=Charged multivesicular body protein 4; Al...   140   7e-32
BT053455_1(BT053455|pid:none) Medicago truncatula clone MTYFP_FQ...    52   3e-05
(Q5KL29) RecName: Full=Vacuolar-sorting protein SNF7; AltName: F...    36   1e-04
FN357406_25(FN357406|pid:none) Schistosoma mansoni genome sequen...    36   7e-04
EF086530_1(EF086530|pid:none) Picea sitchensis clone WS02738_C09...    47   0.001
BT070686_1(BT070686|pid:none) Picea sitchensis clone WS02744_A14...    47   0.001
(Q9SZE4) RecName: Full=Vacuolar protein sorting-associated prote...    44   0.012
AY088519_1(AY088519|pid:none) Arabidopsis thaliana clone 7471 mR...    44   0.012
AM468046_1(AM468046|pid:none) Vitis vinifera contig VV78X022861....    42   0.035
BT038648_1(BT038648|pid:none) Zea mays full-length cDNA clone ZM...    41   0.078
AK065728_1(AK065728|pid:none) Oryza sativa Japonica Group cDNA c...    41   0.078
AE013599_817(AE013599|pid:none) Drosophila melanogaster chromoso...    40   0.13
BT033457_1(BT033457|pid:none) Zea mays full-length cDNA clone ZM...    40   0.17
(Q9P7F7) RecName: Full=Vacuolar-sorting protein snf7; AltName: F...    39   0.23
(Q6GL11) RecName: Full=Charged multivesicular body protein 4b; A...    39   0.23
(Q6CJL0) RecName: Full=Vacuolar-sorting protein SNF7; AltName: F...    39   0.23
T34399(T34399)hypothetical protein C37C3.3 - Caenorhabditis eleg...    39   0.30
EF070538_1(EF070538|pid:none) Maconellicoccus hirsutus clone WHM...    38   0.50
AY558202_1(AY558202|pid:none) Saccharomyces cerevisiae clone FLH...    38   0.66
(Q9H444) RecName: Full=Charged multivesicular body protein 4b; A...    38   0.66
AP008217_107(AP008217|pid:none) Oryza sativa (japonica cultivar-...    38   0.66
(Q6FW96) RecName: Full=Vacuolar-sorting protein SNF7; AltName: F...    37   0.86
(Q6IQ73) RecName: Full=Charged multivesicular body protein 4c; A...    37   0.86
CU928179_849(CU928179|pid:none) Zygosaccharomyces rouxii strain ...    37   1.1
(Q5ZHP5) RecName: Full=Charged multivesicular body protein 4b; A...    37   1.1
CP000500_174(CP000500|pid:none) Pichia stipitis CBS 6054 chromos...    37   1.1
AX885772_1(AX885772|pid:none) Sequence 1635 from Patent EP1033401.     37   1.1
AX885899_1(AX885899|pid:none) Sequence 1762 from Patent EP1033401.     35   3.3
U58762_6(U58762|pid:none) Caenorhabditis elegans cosmid T27F7, c...    35   3.3
AY329084_1(AY329084|pid:none) Homo sapiens SNF7-1 mRNA, complete...    35   3.3
FM992692_227(FM992692|pid:none) Candida dubliniensis CD36 chromo...    35   3.3
(Q9BY43) RecName: Full=Charged multivesicular body protein 4a; A...    35   3.3
BC010893_1(BC010893|pid:none) Homo sapiens chromatin modifying p...    35   3.3
BC113533_1(BC113533|pid:none) Homo sapiens chromatin modifying p...    35   3.3
BX247907_12(BX247907|pid:none) Zebrafish DNA sequence from clone...    35   4.3
AL844185_6(AL844185|pid:none) Zebrafish DNA sequence from clone ...    35   4.3
CP000123_524(CP000123|pid:none) Mycoplasma capricolum subsp. cap...    35   4.3
BX247907_14(BX247907|pid:none) Zebrafish DNA sequence from clone...    35   4.3
CP001326_634(CP001326|pid:none) Micromonas sp. RCC299 chromosome...    35   4.3
(P51834) RecName: Full=Chromosome partition protein smc;  &AL009...    35   4.3
CP000813_3122(CP000813|pid:none) Bacillus pumilus SAFR-032, comp...    35   5.6
BX538352_75(BX538352|pid:none) Cryptosporidium parvum chromosome...    35   5.6
AL844506_103(AL844506|pid:none) Plasmodium falciparum 3D7 chromo...    34   7.3
AE015928_3721(AE015928|pid:none) Bacteroides thetaiotaomicron VP...    34   9.5

>(Q86H98) RecName: Full=Charged multivesicular body protein 4;
           AltName: Full=Vacuolar protein-sorting-associated
           protein 32;          Short=Dd-Vps32;
           &AC116960_10(AC116960|pid:none)
          Length = 215

 Score =  140 bits (353), Expect = 7e-32
 Identities = 107/219 (48%), Positives = 127/219 (57%), Gaps = 4/219 (1%)
 Frame = +2

Query: 197 QNQNKILKKQLVNLD*H*ICLKKGKHFYKIN*KKRTRKSKGL*YSPKKERETHLLCLKKS 376
           Q+  + + K  + LD     L+K + F +   +K   K+K L  S K++RE  LLCLKK
Sbjct: 11  QDTQETIGKLRLTLD----MLEKRQTFLQDKLEKEQEKAKVL-VSQKRKREA-LLCLKKR 64

Query: 377 N*FSK*GYKITKVHMIH*T--QQIFALENAKMKYGNYEI--PMRDGARSIKRIPMVTLLL 544
           N F     ++TK+   + T  QQIFALENAKM   N EI   MRDGARS++ +     L
Sbjct: 65  NNFQN---EVTKLQGSYDTLNQQIFALENAKM---NMEIMNSMRDGARSLRELH--GHLT 116

Query: 545 SIKLMT*IEKIQEQMEIHEEISNAISQPLGNQVXXXXXXXXXXXXXXXXXXXXXXXNIKA 724
             K+   IE+IQEQMEIHEEISNAISQPLGNQV                       NIKA
Sbjct: 117 IDKVDDVIEEIQEQMEIHEEISNAISQPLGNQVEDEEELLRELAEYEQEELDAQLLNIKA 176

Query: 725 PSRELPQEIQVNFPTISKTVPKLSKXXXXXXXXXXSLAM 841
           PSRELPQEIQVNFPTISKTVPKLSK          SLAM
Sbjct: 177 PSRELPQEIQVNFPTISKTVPKLSKEEEEIRALEESLAM 215

 Score =  114 bits (284), Expect = 7e-24
 Identities = 68/121 (56%), Positives = 82/121 (67%)
 Frame = +1

Query: 178 MKLFGKPKPKQDTQETIGKLRLTLDMLEKRQTFLQDKLEKENKKKQRSLVQPKKRKRDAF 357
           MKLFGKPKPKQDTQETIGKLRLTLDMLEKRQTFLQDKLEKE +K +  ++  +KRKR+A
Sbjct: 1   MKLFGKPKPKQDTQETIGKLRLTLDMLEKRQTFLQDKLEKEQEKAK--VLVSQKRKREAL 58

Query: 358 IMFKKEQLIFKMRLQNYQGSYDTLNSTNFCIRECKDEIWQL*NSNERWCKIH*ENSHGHL 537
           +  KK    F+  +   QGSYDTLN   F +   K  + ++ NS     +   E  HGHL
Sbjct: 59  LCLKKRN-NFQNEVTKLQGSYDTLNQQIFALENAKMNM-EIMNSMRDGARSLRE-LHGHL 115

Query: 538 T 540
           T
Sbjct: 116 T 116

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 995,857,031
Number of extensions: 15946308
Number of successful extensions: 49923
Number of sequences better than 10.0: 44
Number of HSP's gapped: 48967
Number of HSP's successfully gapped: 47
Length of query: 341
Length of database: 1,061,185,681
Length adjustment: 129
Effective length of query: 212
Effective length of database: 639,555,889
Effective search space: 135585848468
Effective search space used: 135585848468
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.56  gvh: 0.35  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.42  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U05086-1 is nuc
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 6
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0