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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U05022-1
Contig ID Contig-U05022-1
Contig update 2001. 8.29
Contig sequence 
>Contig-U05022-1 (Contig-U05022-1Q) /CSM_Contig/Contig-U05022-1Q.Seq.d
AACAATATTTTACACAATTTTATGGGGCAAAGAGAACCTATCAACAATTT
GGAGATAAATATCAAGATGAAAAGAATTATGCAGATTTAATACCTTCACA
ATATCACGATTTTAATACAACAGGATCACAAAATCAACGTGATCGTGTAG
TTAGAGGCAATAAAACATTAGAGAATGGCATTCAAATGATTAGAATGGCA
AACGATCAAGTTAGAGAGGATAAGAAAACAGCAGAATCCACATTGGAACA
ATTGGCAACTCAAAAAGAGCGTTTAATAGGTTTCGAAAAGAAATTCGATT
CAATCGATGGTTATTTAGATAAAGCAAAGAAAACAATTGGTCAAATGTCA
AGAAGGGCAATGGCAAATAAATTCATTATGGCTGGTATAATTTTCATCTT
ATTAATTGCAATTGGTTTAATCATTTGGTTGAAATTCGTTAGAACTGGTG
ATTCAAATAATAATAGTAGTAGTAGTAGTAGTTTTGAAACTACTACCACT
ACCTCCACCACTGGTACTACTTCAACAACTTCTTCTACTTCCACTACTAC
TGGTACTACTACTTCAGATCCTGCAACTGGTTCATCAACTACTACCAGTG
ATACAAAAATATAAATAATAAAAAAAAAAAAAAAGAAAAGAAAAGGAAAA
AAAAAAAAAAAATGAA
Gap no gap
Contig length 666
Chromosome number (1..6, M) 4
Chromosome length 5430582
Start point 587231
End point 587897
Strand (PLUS/MINUS) PLUS
Number of clones 3
Number of EST 3
Link to clone list U05022
List of clone(s) 

est1=SSM133Z,1,619
est2=SSG782Z,25,667
est3=SSE575Z,123,660
Translated Amino Acid sequence 
QYFTQFYGAKRTYQQFGDKYQDEKNYADLIPSQYHDFNTTGSQNQRDRVVRGNKTLENGI
QMIRMANDQVREDKKTAESTLEQLATQKERLIGFEKKFDSIDGYLDKAKKTIGQMSRRAM
ANKFIMAGIIFILLIAIGLIIWLKFVRTGDSNNNSSSSSSFETTTTTSTTGTTSTTSSTS
TTTGTTTSDPATGSSTTTSDTKI*iikkkkkkrkgkkkkk*
Translated Amino Acid sequence (All Frames) 
Frame A:
nnilhnfmgqrepinnleinikmkrimqi*ylhnitiliqqdhkinviv*leaikh*rma
fk*lewqtiklerirkqqnphwnnwqlkksv**vskrnsiqsmvi*ikqrkqlvkcqegq
wqinslwlv*fssy*lqlv*sfg*nslelviqiiivvvvvvlkllplppplvllqqllll
plllvlllqilqlvhqllpviqkyk**kkkkrkekekkkkne


Frame B:
tifytilwgkenlstiwr*isr*kelcrfntftisrf*ynritkst*scs*rq*nirewh
snd*ngkrss*rg*ensrihigtignskrafnrfrkeirfnrwlfr*skennwsnvkkgn
gk*ihygwynfhlincnwfnhlveir*nw*fk********f*nyyhylhhwyyfnnffyf
hyywyyyfrscnwfinyyq*ykninnkkkkkekkrkkkkkm


Frame C:
QYFTQFYGAKRTYQQFGDKYQDEKNYADLIPSQYHDFNTTGSQNQRDRVVRGNKTLENGI
QMIRMANDQVREDKKTAESTLEQLATQKERLIGFEKKFDSIDGYLDKAKKTIGQMSRRAM
ANKFIMAGIIFILLIAIGLIIWLKFVRTGDSNNNSSSSSSFETTTTTSTTGTTSTTSSTS
TTTGTTTSDPATGSSTTTSDTKI*iikkkkkkrkgkkkkk*
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U05022-1 (Contig-U05022-1Q)
/CSM_Contig/Contig-U05022-1Q.Seq.d
         (666 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U05022-1 (Contig-U05022-1Q) /CSM_Contig/Conti...   918  0.0
Contig-U12216-1 (Contig-U12216-1Q) /CSM_Contig/Conti...    38  0.012
Contig-U11102-1 (Contig-U11102-1Q) /CSM_Contig/Conti...    38  0.012
Contig-U04744-1 (Contig-U04744-1Q) /CSM_Contig/Conti...    38  0.012
Contig-U14331-1 (Contig-U14331-1Q) /CSM_Contig/Conti...    36  0.047
Contig-U13395-1 (Contig-U13395-1Q) /CSM_Contig/Conti...    36  0.047
Contig-U11408-1 (Contig-U11408-1Q) /CSM_Contig/Conti...    36  0.047
Contig-U13880-1 (Contig-U13880-1Q) /CSM_Contig/Conti...    34  0.18
Contig-U09753-1 (Contig-U09753-1Q) /CSM_Contig/Conti...    34  0.18
Contig-U09628-1 (Contig-U09628-1Q) /CSM_Contig/Conti...    34  0.18

>Contig-U05022-1 (Contig-U05022-1Q) /CSM_Contig/Contig-U05022-1Q.Seq.d
          Length = 666

 Score =  918 bits (463), Expect = 0.0
 Identities = 463/463 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   aacaatattttacacaattttatggggcaaagagaacctatcaacaatttggagataaat 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   aacaatattttacacaattttatggggcaaagagaacctatcaacaatttggagataaat 60

                                                                       
Query: 61  atcaagatgaaaagaattatgcagatttaataccttcacaatatcacgattttaatacaa 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  atcaagatgaaaagaattatgcagatttaataccttcacaatatcacgattttaatacaa 120

                                                                       
Query: 121 caggatcacaaaatcaacgtgatcgtgtagttagaggcaataaaacattagagaatggca 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 caggatcacaaaatcaacgtgatcgtgtagttagaggcaataaaacattagagaatggca 180

                                                                       
Query: 181 ttcaaatgattagaatggcaaacgatcaagttagagaggataagaaaacagcagaatcca 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 ttcaaatgattagaatggcaaacgatcaagttagagaggataagaaaacagcagaatcca 240

                                                                       
Query: 241 cattggaacaattggcaactcaaaaagagcgtttaataggtttcgaaaagaaattcgatt 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 cattggaacaattggcaactcaaaaagagcgtttaataggtttcgaaaagaaattcgatt 300

                                                                       
Query: 301 caatcgatggttatttagataaagcaaagaaaacaattggtcaaatgtcaagaagggcaa 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 caatcgatggttatttagataaagcaaagaaaacaattggtcaaatgtcaagaagggcaa 360

                                                                       
Query: 361 tggcaaataaattcattatggctggtataattttcatcttattaattgcaattggtttaa 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 tggcaaataaattcattatggctggtataattttcatcttattaattgcaattggtttaa 420

                                                      
Query: 421 tcatttggttgaaattcgttagaactggtgattcaaataataa 463
           |||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 tcatttggttgaaattcgttagaactggtgattcaaataataa 463


 Score =  107 bits (54), Expect = 2e-23
 Identities = 54/54 (100%)
 Strand = Plus / Plus

                                                                 
Query: 566 agatcctgcaactggttcatcaactactaccagtgatacaaaaatataaataat 619
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 566 agatcctgcaactggttcatcaactactaccagtgatacaaaaatataaataat 619


 Score = 63.9 bits (32), Expect = 2e-10
 Identities = 32/32 (100%)
 Strand = Plus / Plus

                                           
Query: 483 tttgaaactactaccactacctccaccactgg 514
           ||||||||||||||||||||||||||||||||
Sbjct: 483 tttgaaactactaccactacctccaccactgg 514


>Contig-U12216-1 (Contig-U12216-1Q) /CSM_Contig/Contig-U12216-1Q.Seq.d
          Length = 1548

 Score = 38.2 bits (19), Expect = 0.012
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 487 aaactactaccactacctc 505
           |||||||||||||||||||
Sbjct: 313 aaactactaccactacctc 331


>Contig-U11102-1 (Contig-U11102-1Q) /CSM_Contig/Contig-U11102-1Q.Seq.d
          Length = 3887

 Score = 38.2 bits (19), Expect = 0.012
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 58  aatatcaagatgaaaagaa 76
           |||||||||||||||||||
Sbjct: 966 aatatcaagatgaaaagaa 984


 Score = 30.2 bits (15), Expect = 2.9
 Identities = 15/15 (100%)
 Strand = Plus / Plus

                           
Query: 64   aagatgaaaagaatt 78
            |||||||||||||||
Sbjct: 1035 aagatgaaaagaatt 1049


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 7893
Number of Sequences: 6905
Number of extensions: 7893
Number of successful extensions: 992
Number of sequences better than 10.0: 113
length of query: 666
length of database: 5,674,871
effective HSP length: 16
effective length of query: 650
effective length of database: 5,564,391
effective search space: 3616854150
effective search space used: 3616854150
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 7. 8
Homology vs DNA 
Query= Contig-U05022-1 (Contig-U05022-1Q) /CSM_Contig/Contig-U05022-1Q.Seq.d
         (666 letters)

Database: ddbj_B
           105,743,758 sequences; 104,622,809,269 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU038913) Dictyostelium discoideum slug cDNA, clone SSM133.      918   0.0    2
(C89767) Dictyostelium discoideum slug cDNA, clone SSG782.        841   0.0    2
(C92527) Dictyostelium discoideum slug cDNA, clone SSE575.        676   0.0    2
(GM887597) Sequence 64 from Patent WO2008137089.                   46   1.8    1
(GM887560) Sequence 27 from Patent WO2008137089.                   46   1.8    1
(AF107885) Homo sapiens chromosome 14q24.3 clone BAC270M14 t...    46   1.8    1
(AC009399) Homo sapiens chromosome 14 clone RP11-368K8 conta...    46   1.8    1
(AC155456) Zea mays strain B73 clone ZMMBBb0448P23, *** SEQU...    46   1.8    1
(AC155430) Zea mays strain B73 clone ZMMBBb0237K19, *** SEQU...    46   1.8    1
(AC213515) Zea mays chromosome unknown clone CH201-33M17; ZM...    46   1.8    1
(AC212221) Zea mays chromosome unknown clone CH201-305P9; ZM...    46   1.8    1
(AC198693) Zea mays chromosome 3 clone CH201-331P11; ZMMBBc0...    46   1.8    1
(AC197842) Zea mays chromosome 1 clone CH201-98B12; ZMMBBc00...    46   1.8    1
(AC190839) Zea mays chromosome 1 clone ZMMBBb-448P23; ZMMBBb...    46   1.8    1
(CG446267) OGVHF51TH ZM_0.7_1.5_KB Zea mays genomic clone ZM...    46   1.8    1
(CC736399) PUIAD16TB ZM_0.6_1.0_KB Zea mays genomic clone ZM...    46   1.8    1
(CJ768348) Ipomoea nil cDNA clone:jmsf5e09, 3' end.                46   1.8    1
(CJ764646) Ipomoea nil cDNA clone:jmsf34b20, 3' end.               46   1.8    1
(CJ758492) Ipomoea nil cDNA clone:jmsf18g24, 3' end.               46   1.8    1
(FL657529) Embrapa_Musa_ABB_NonStressed_Cachaco_N529TF Embra...    46   1.8    1
(AC191757) Pan troglodytes BAC clone CH251-412K11 from chrom...    40   1.8    5
(EU677193) Oedogonium cardiacum strain SAG 575-1b chloroplas...    34   2.3    8
(AC173771) Bos taurus clone CH240-209L19, WORKING DRAFT SEQU...    40   2.4    3
(AL157775) Human DNA sequence from clone RP11-525O21 on chro...    44   2.4    2
(CT940636) Equus caballus GSS, BAC clone CH241-21L4, SP6 end...    34   4.7    2
(AP008212) Oryza sativa (japonica cultivar-group) genomic DN...    44   6.9    1
(AP005688) Oryza sativa Japonica Group genomic DNA, chromoso...    44   6.9    1
(AM453135) Vitis vinifera contig VV78X099943.21, whole genom...    44   6.9    1
(EF170288) Sus scrofa clone KVL14196 microsatellite sequence.      44   6.9    1
(AC117081) Dictyostelium discoideum chromosome 2 map 5862124...    44   6.9    1
(AL139335) Human DNA sequence *** SEQUENCING CANCELLED *** f...    44   6.9    1
(AC234479) Tupaia belangeri clone CH275-426J8, WORKING DRAFT...    44   6.9    1
(AC231560) Tupaia belangeri clone CH275-557H2, WORKING DRAFT...    44   6.9    1
(AC023009) Homo sapiens chromosome 6 clone RP11-10O2 map 6, ...    44   6.9    1
(ET036434) MUGQ_CH252P339P06Sp6_CP630_017 CHORI-252 Vervet M...    44   6.9    1
(EJ025806) 1095454023628 Global-Ocean-Sampling_GS-26-01-01-1...    44   6.9    1
(CU965532) Brassica napus GSS, clone JBnB129G24, end read, p...    44   6.9    1
(CL017095) CH216-1K14_RM1.1 CH216 Xenopus (Silurana) tropica...    44   6.9    1
(BZ077155) lle39b07.b1 B.oleracea002 Brassica oleracea genom...    44   6.9    1
(AU071832) Dictyostelium discoideum slug cDNA, clone SSC606.       44   6.9    1
(CV286904) 67969.1 After-Cooking Darkening A Solanum tuberos...    44   6.9    1
(CP001612) Rickettsia africae ESF-5, complete genome.              44   6.9    1
(AE006914) Rickettsia conorii str. Malish 7, complete genome.      44   6.9    1

>(AU038913) Dictyostelium discoideum slug cDNA, clone SSM133.
          Length = 619

 Score =  918 bits (463), Expect(2) = 0.0
 Identities = 463/463 (100%)
 Strand = Plus / Plus


Query: 1   aacaatattttacacaattttatggggcaaagagaacctatcaacaatttggagataaat 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   aacaatattttacacaattttatggggcaaagagaacctatcaacaatttggagataaat 60


Query: 61  atcaagatgaaaagaattatgcagatttaataccttcacaatatcacgattttaatacaa 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  atcaagatgaaaagaattatgcagatttaataccttcacaatatcacgattttaatacaa 120


Query: 121 caggatcacaaaatcaacgtgatcgtgtagttagaggcaataaaacattagagaatggca 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 caggatcacaaaatcaacgtgatcgtgtagttagaggcaataaaacattagagaatggca 180


Query: 181 ttcaaatgattagaatggcaaacgatcaagttagagaggataagaaaacagcagaatcca 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 ttcaaatgattagaatggcaaacgatcaagttagagaggataagaaaacagcagaatcca 240


Query: 241 cattggaacaattggcaactcaaaaagagcgtttaataggtttcgaaaagaaattcgatt 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 cattggaacaattggcaactcaaaaagagcgtttaataggtttcgaaaagaaattcgatt 300


Query: 301 caatcgatggttatttagataaagcaaagaaaacaattggtcaaatgtcaagaagggcaa 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 caatcgatggttatttagataaagcaaagaaaacaattggtcaaatgtcaagaagggcaa 360


Query: 361 tggcaaataaattcattatggctggtataattttcatcttattaattgcaattggtttaa 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 tggcaaataaattcattatggctggtataattttcatcttattaattgcaattggtttaa 420


Query: 421 tcatttggttgaaattcgttagaactggtgattcaaataataa 463
           |||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 tcatttggttgaaattcgttagaactggtgattcaaataataa 463

 Score =  107 bits (54), Expect(2) = 0.0
 Identities = 54/54 (100%)
 Strand = Plus / Plus


Query: 566 agatcctgcaactggttcatcaactactaccagtgatacaaaaatataaataat 619
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 566 agatcctgcaactggttcatcaactactaccagtgatacaaaaatataaataat 619

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 105743758
Number of Hits to DB: 706,206,215
Number of extensions: 40782070
Number of successful extensions: 3209964
Number of sequences better than 10.0: 43
Length of query: 666
Length of database: 104,622,809,269
Length adjustment: 24
Effective length of query: 642
Effective length of database: 102,084,959,077
Effective search space: 65538543727434
Effective search space used: 65538543727434
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.31
Homology vs Protein 
Query= Contig-U05022-1 (Contig-U05022-1Q) /CSM_Contig/Contig-U05022-1Q.Seq.d
         (666 letters)

Database: nrp_A
           3,268,448 sequences; 1,061,185,681 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR382136_498(CR382136|pid:none) Debaryomyces hansenii strain CBS...    60   8e-08
FM992690_320(FM992690|pid:none) Candida dubliniensis CD36 chromo...    47   7e-04
AF114751_1(AF114751|pid:none) Arabidopsis thaliana v-SNARE AtVTI...    46   0.001
AB279873_1(AB279873|pid:none) Aspergillus oryzae Aovti1 gene for...    45   0.003
CR382128_87(CR382128|pid:none) Yarrowia lipolytica strain CLIB12...    44   0.003
(Q04338) RecName: Full=t-SNARE VTI1; AltName: Full=Qb-SNARE VTI1...    43   0.010
AB261079_1(AB261079|pid:none) Entamoeba histolytica gene for EhS...    43   0.010
CU928175_236(CU928175|pid:none) Zygosaccharomyces rouxii strain ...    42   0.016
CR380958_453(CR380958|pid:none) Candida glabrata strain CBS138 c...    42   0.016
AK102003_1(AK102003|pid:none) Oryza sativa Japonica Group cDNA c...    41   0.036
BC055131_1(BC055131|pid:none) Danio rerio vesicle transport thro...    41   0.036
EF144816_1(EF144816|pid:none) Populus trichocarpa clone WS01118_...    41   0.036
EU552421_3(EU552421|pid:none) Starmerella bombicola putative F-b...    40   0.081
BC121621_1(BC121621|pid:none) Xenopus tropicalis hypothetical pr...    39   0.11
AK118409_1(AK118409|pid:none) Arabidopsis thaliana At1g26670 mRN...    39   0.11
BC073454_1(BC073454|pid:none) Xenopus laevis MGC80961 protein, m...    39   0.14
AB027611_1(AB027611|pid:none) Xenopus laevis mRNA for cortactin,...    38   0.24
BC169873_1(BC169873|pid:none) Xenopus laevis cortactin, mRNA (cD...    38   0.24
AM412317_3133(AM412317|pid:none) Clostridium botulinum A str. AT...    37   0.53
CP000939_3134(CP000939|pid:none) Clostridium botulinum B1 str. O...    37   0.53
CR382122_417(CR382122|pid:none) Kluyveromyces lactis strain NRRL...    37   0.53
AK303646_1(AK303646|pid:none) Homo sapiens cDNA FLJ61523 complet...    37   0.53
BC167645_1(BC167645|pid:none) Xenopus tropicalis cortactin, mRNA...    37   0.69
AL139120_2(AL139120|pid:none) Human DNA sequence from clone RP11...    36   1.2
(O14139) RecName: Full=Chromodomain helicase hrp3;          EC=3...    35   1.5
CP000728_3034(CP000728|pid:none) Clostridium botulinum F str. La...    35   2.0
CP001083_3261(CP001083|pid:none) Clostridium botulinum Ba4 str. ...    35   2.6
CR954204_232(CR954204|pid:none) Ostreococcus tauri strain OTTH05...    35   2.6
DQ864787_1(DQ864787|pid:none) Pfiesteria piscicida clone ppi-5p-...    34   3.4
CU633901_229(CU633901|pid:none) Podospora anserina genomic DNA c...    34   3.4
CP000962_3111(CP000962|pid:none) Clostridium botulinum A3 str. L...    34   4.5
AF326566_1(AF326566|pid:none) Streptococcus pyogenes M18 protein...    34   4.5
AP002527_83(AP002527|pid:none) Escherichia coli plasmid R721 gen...    34   4.5
(Q96AJ9) RecName: Full=Vesicle transport through interaction wit...    34   4.5
CR380956_330(CR380956|pid:none) Candida glabrata strain CBS138 c...    33   5.8
(P83351) RecName: Full=Uncharacterized protein K02D10.5;  &L1471...    33   7.6
AC002304_7(AC002304|pid:none) Genomic sequence for Arabidopsis t...    33   7.6
S44837(S44837)K02D10.1 protein - Caenorhabditis elegans                33   7.6
EU957686_1(EU957686|pid:none) Zea mays clone 1604248 unknown mRNA.     33   7.6
CP000679_2222(CP000679|pid:none) Caldicellulosiruptor saccharoly...    33   9.9
AM910986_143(AM910986|pid:none) Plasmodium knowlesi strain H chr...    33   9.9

>CR382136_498(CR382136|pid:none) Debaryomyces hansenii strain CBS767
           chromosome A complete sequence.
          Length = 220

 Score = 59.7 bits (143), Expect = 8e-08
 Identities = 34/111 (30%), Positives = 59/111 (53%)
 Frame = +3

Query: 39  YQQFGDKYQDEKNYADLIPSQYHDFNTTGSQNQRDRVVRGNKTLENGIQMIRMANDQVRE 218
           Y+ FG++Y D+ ++ D+     HD       +QR +++  N +LE   + +R +     E
Sbjct: 99  YELFGNRYTDDDSHDDI-----HD-------SQRKQLLNNNSSLERTSERLRDSQRIALE 146

Query: 219 DKKTAESTLEQLATQKERLIGFEKKFDSIDGYLDKAKKTIGQMSRRAMANK 371
            +    + L  L +Q+E++ G      + DGY+DK+ KT+  MSRR  ANK
Sbjct: 147 TENIGGNILNDLRSQREQITGSRNTLMTADGYVDKSIKTLKSMSRRLTANK 197

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 770,541,008
Number of extensions: 12630139
Number of successful extensions: 31887
Number of sequences better than 10.0: 41
Number of HSP's gapped: 31865
Number of HSP's successfully gapped: 41
Length of query: 222
Length of database: 1,061,185,681
Length adjustment: 124
Effective length of query: 98
Effective length of database: 655,898,129
Effective search space: 64278016642
Effective search space used: 64278016642
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 31 (16.5 bits)
PSORT 

 psg: 0.54  gvh: 0.25  alm: 0.10  top: 0.27  tms: 0.07  mit: 0.29  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: nuclear
	 20.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 20.0 %: endoplasmic reticulum
	  8.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for Contig-U05022-1 is nuc
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 3
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0