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Dicty_cDB
Contig List | Clone List | cDNA Library List
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U04637-1
Contig ID Contig-U04637-1
Contig update 2001. 8.29
Contig sequence 
>Contig-U04637-1 (Contig-U04637-1Q) /CSM_Contig/Contig-U04637-1Q.Seq.d
CTTTTTTGTTATTAATTTTCATTTCCATATTAATTTTTTGAAAAAAAAAA
AAAAGTAAAAAATTGTTTAGAAAAAAAAAAAAATAAAATAAAAAGTTGTT
TAAAAAAAAAAAAAATGTTTAGATTTTTATTTGTAATCATTGTAATATTA
ATTGCAATTTCGGGTAATAGTATTGTTTCCTCAAATGAAGTTGCCCAATG
TGATGTTTGTAATTATTTAGTAACAATGGTAGAAGTTTTCGTTGAACAAA
ATAGAAGTGAAACTTATATTTCAAACTCTCTTGAAAAAGTTTGTGAAATC
ATTCCAAGAGAAGATTATAAATCAACTTGTAGATCAATTGTTTTAGCATA
TACAAAAGATATTATTCAATTAATTATTAATAGAGAACCATCAGAAAAAA
TTTGTCAAGAGATTAAAGCTTGTCCATTACCAACCCCTTCTCCATCGCCA
TCCAAACAAGTTAAACCAAAAAGAAATGAAGATGATGCTCTACAATGTAC
AATTTGTAAATTGGTTGCCACTAAATTAGAAGAGTACATTCAATCAAATA
AAACCATTGAAGAGATTGAAAAATGAATTGGATGATTTCTGTAAAATTGC
TTTTGAAAAAGATCCAACCCAATGCCAAGGTTTTGTACAACAATATGTTC
CAATGATTTTATCTTTTATCAAGAGTAAAAAGAGGATCCAACTCAAGCTT
GCATTAAATTAAAATTCTGTACATCTTCAAGAGTAACTAAAGCAATTCTT
AGTCATCATGTTTTGATTAATAAGAATTATTAAAACATTAATTAAAAAAA
TAAAAAAATAAAAAATAAAAAATAAATAATAAAGAAATAATAAAAATTTA
AATAAAATCTATCTATCCTATCCATGTATATACATAAATAAAAA
Gap no gap
Contig length 894
Chromosome number (1..6, M) 3
Chromosome length 6358359
Start point 2437919
End point 2437024
Strand (PLUS/MINUS) MINUS
Number of clones 8
Number of EST 8
Link to clone list U04637
List of clone(s) 

est1=SSB889E,1,853
est2=SSE704F,47,466
est3=SSJ869Z,229,818
est4=SSH403Z,330,895
est5=SSG441Z,372,869
est6=SSH302Z,387,834
est7=SSC244Z,399,864
est8=SSM477E,419,860
Translated Amino Acid sequence 
lfcy*fsfpy*FFEKKKKVKNCLEKKKNKIKSCLKKKKMFRFLFVIIVILIAISGNSIVS
SNEVAQCDVCNYLVTMVEVFVEQNRSETYISNSLEKVCEIIPREDYKSTCRSIVLAYTKD
IIQLIINREPSEKICQEIKACPLPTPSPSPSKQVKPKRNEDDALQCTICKLVATKLEEYI
QSNKTIEEIEK*ig*fl*ncf*krsnpmprfctticsndfifyqe*kedptqaciklkfc
tssrvtkailshhvlinkny*nin*knkkiknkk*iikk**kfk*nlsilsmyihk*k
Translated Amino Acid sequence (All Frames) 
Frame A:
lfcy*fsfpy*FFEKKKKVKNCLEKKKNKIKSCLKKKKMFRFLFVIIVILIAISGNSIVS
SNEVAQCDVCNYLVTMVEVFVEQNRSETYISNSLEKVCEIIPREDYKSTCRSIVLAYTKD
IIQLIINREPSEKICQEIKACPLPTPSPSPSKQVKPKRNEDDALQCTICKLVATKLEEYI
QSNKTIEEIEK*ig*fl*ncf*krsnpmprfctticsndfifyqe*kedptqaciklkfc
tssrvtkailshhvlinkny*nin*knkkiknkk*iikk**kfk*nlsilsmyihk*k


Frame B:
ffvinfhfhinflkkkkk*kiv*kkkkik*kvv*kkkkcldfyl*sl*y*lqfrvivlfp
qmklpnvmfvii**qw*kfslnkievklifqtllkkfvksfqekiinqlvdqlf*hiqki
lfn*llienhqkkfvkrlklvhyqpllhrhpnklnqkemkmmlynvqfvnwlpln*kstf
nqikplkrlknelddfckiafekdptqcqgfvqqyvpmilsfikskkriqlklaln*nsv
hlqe*lkqflvimf*liriiktlikkikk*kiknk**rnnknlnkiylsypciyink


Frame C:
flllifisilif*kkkkskklfrkkkk*nkklfkkkknv*ificnhcnincnfg**ycfl
k*scpm*cl*lfsnngrsfr*tk*k*nlyfkls*ksl*nhskrrl*inl*incfsiykry
ysiny**rtirknlsrd*slsitnpfsiaiqts*tkkk*r*cstmynl*igch*irrvhs
ik*nh*rd*kmnwmisvklllkkiqpnakvlynnmfq*fyllsrvkrgsnsslh*ikily
ifksn*sns*sscfd**ellkh*lkk*knkk*kinnkeiiki*iksiypihvyt*ik
own update 2004. 6.10
Homology vs CSM-cDNA 
Query= Contig-U04637-1 (Contig-U04637-1Q)
/CSM_Contig/Contig-U04637-1Q.Seq.d
         (894 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U04637-1 (Contig-U04637-1Q) /CSM_Contig/Conti...  1342  0.0
Contig-U00307-1 (Contig-U00307-1Q) /CSM_Contig/Conti...    38  0.016
Contig-U14495-1 (Contig-U14495-1Q) /CSM_Contig/Conti...    36  0.063
Contig-U11831-1 (Contig-U11831-1Q) /CSM_Contig/Conti...    36  0.063
Contig-U11448-1 (Contig-U11448-1Q) /CSM_Contig/Conti...    36  0.063
Contig-U09552-1 (Contig-U09552-1Q) /CSM_Contig/Conti...    36  0.063
Contig-U07096-1 (Contig-U07096-1Q) /CSM_Contig/Conti...    36  0.063
Contig-U04851-1 (Contig-U04851-1Q) /CSM_Contig/Conti...    36  0.063
Contig-U02102-1 (Contig-U02102-1Q) /CSM_Contig/Conti...    36  0.063
Contig-U14173-1 (Contig-U14173-1Q) /CSM_Contig/Conti...    34  0.25

>Contig-U04637-1 (Contig-U04637-1Q) /CSM_Contig/Contig-U04637-1Q.Seq.d
          Length = 894

 Score = 1342 bits (677), Expect = 0.0
 Identities = 677/677 (100%)
 Strand = Plus / Plus

                                                                       
Query: 116 tgtttagatttttatttgtaatcattgtaatattaattgcaatttcgggtaatagtattg 175
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 116 tgtttagatttttatttgtaatcattgtaatattaattgcaatttcgggtaatagtattg 175

                                                                       
Query: 176 tttcctcaaatgaagttgcccaatgtgatgtttgtaattatttagtaacaatggtagaag 235
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 176 tttcctcaaatgaagttgcccaatgtgatgtttgtaattatttagtaacaatggtagaag 235

                                                                       
Query: 236 ttttcgttgaacaaaatagaagtgaaacttatatttcaaactctcttgaaaaagtttgtg 295
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 236 ttttcgttgaacaaaatagaagtgaaacttatatttcaaactctcttgaaaaagtttgtg 295

                                                                       
Query: 296 aaatcattccaagagaagattataaatcaacttgtagatcaattgttttagcatatacaa 355
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 296 aaatcattccaagagaagattataaatcaacttgtagatcaattgttttagcatatacaa 355

                                                                       
Query: 356 aagatattattcaattaattattaatagagaaccatcagaaaaaatttgtcaagagatta 415
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 356 aagatattattcaattaattattaatagagaaccatcagaaaaaatttgtcaagagatta 415

                                                                       
Query: 416 aagcttgtccattaccaaccccttctccatcgccatccaaacaagttaaaccaaaaagaa 475
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 416 aagcttgtccattaccaaccccttctccatcgccatccaaacaagttaaaccaaaaagaa 475

                                                                       
Query: 476 atgaagatgatgctctacaatgtacaatttgtaaattggttgccactaaattagaagagt 535
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 476 atgaagatgatgctctacaatgtacaatttgtaaattggttgccactaaattagaagagt 535

                                                                       
Query: 536 acattcaatcaaataaaaccattgaagagattgaaaaatgaattggatgatttctgtaaa 595
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 536 acattcaatcaaataaaaccattgaagagattgaaaaatgaattggatgatttctgtaaa 595

                                                                       
Query: 596 attgcttttgaaaaagatccaacccaatgccaaggttttgtacaacaatatgttccaatg 655
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 596 attgcttttgaaaaagatccaacccaatgccaaggttttgtacaacaatatgttccaatg 655

                                                                       
Query: 656 attttatcttttatcaagagtaaaaagaggatccaactcaagcttgcattaaattaaaat 715
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 656 attttatcttttatcaagagtaaaaagaggatccaactcaagcttgcattaaattaaaat 715

                                                                       
Query: 716 tctgtacatcttcaagagtaactaaagcaattcttagtcatcatgttttgattaataaga 775
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 716 tctgtacatcttcaagagtaactaaagcaattcttagtcatcatgttttgattaataaga 775

                            
Query: 776 attattaaaacattaat 792
           |||||||||||||||||
Sbjct: 776 attattaaaacattaat 792


 Score = 79.8 bits (40), Expect = 5e-15
 Identities = 40/40 (100%)
 Strand = Plus / Plus

                                                  
Query: 1  cttttttgttattaattttcatttccatattaattttttg 40
          ||||||||||||||||||||||||||||||||||||||||
Sbjct: 1  cttttttgttattaattttcatttccatattaattttttg 40


 Score = 71.9 bits (36), Expect = 1e-12
 Identities = 36/36 (100%)
 Strand = Plus / Plus

                                               
Query: 859 ctatctatcctatccatgtatatacataaataaaaa 894
           ||||||||||||||||||||||||||||||||||||
Sbjct: 859 ctatctatcctatccatgtatatacataaataaaaa 894


>Contig-U00307-1 (Contig-U00307-1Q) /CSM_Contig/Contig-U00307-1Q.Seq.d
          Length = 1509

 Score = 38.2 bits (19), Expect = 0.016
 Identities = 22/23 (95%)
 Strand = Plus / Plus

                                  
Query: 548 ataaaaccattgaagagattgaa 570
           ||||| |||||||||||||||||
Sbjct: 751 ataaatccattgaagagattgaa 773


>Contig-U14495-1 (Contig-U14495-1Q) /CSM_Contig/Contig-U14495-1Q.Seq.d
          Length = 483

 Score = 36.2 bits (18), Expect = 0.063
 Identities = 21/22 (95%)
 Strand = Plus / Plus

                                 
Query: 272 caaactctcttgaaaaagtttg 293
           |||| |||||||||||||||||
Sbjct: 129 caaaatctcttgaaaaagtttg 150


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 14,977
Number of Sequences: 6905
Number of extensions: 14977
Number of successful extensions: 1399
Number of sequences better than 10.0: 133
length of query: 894
length of database: 5,674,871
effective HSP length: 16
effective length of query: 878
effective length of database: 5,564,391
effective search space: 4885535298
effective search space used: 4885535298
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 6.27
Homology vs DNA 
Query= Contig-U04637-1 (Contig-U04637-1Q) /CSM_Contig/Contig-U04637-1Q.Seq.d
         (894 letters)

Database: ddbj_A
           102,105,510 sequences; 101,790,757,118 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(C90721) Dictyostelium discoideum slug cDNA, clone SSJ869.        618   0.0    3
(AU038197) Dictyostelium discoideum slug cDNA, clone SSH403.      466   0.0    3
(C94127) Dictyostelium discoideum slug cDNA, clone SSG441.        383   0.0    3
(AU038122) Dictyostelium discoideum slug cDNA, clone SSH302.      351   0.0    3
(C84016) Dictyostelium discoideum slug cDNA, clone SSC244.        321   0.0    3
(AU039174) Dictyostelium discoideum slug cDNA, clone SSM477.      289   0.0    3
(AU036970) Dictyostelium discoideum slug cDNA, clone SSB889.      278   e-132  2
(AU072492) Dictyostelium discoideum slug cDNA, clone SSE704.      339   1e-88  1
(AU071610) Dictyostelium discoideum slug cDNA, clone SSB889.      250   8e-62  1
(GC759133) Sequence 74378 from patent US 6812339.                  50   1e-04  2
(GC759132) Sequence 74377 from patent US 6812339.                  50   1e-04  2
(DJ441102) Breast Cancer Biomarkers.                               54   0.009  1
(DJ441101) Breast Cancer Biomarkers.                               54   0.009  1
(CS200153) Sequence 34 from Patent WO2005106044.                   54   0.009  1
(CS200152) Sequence 33 from Patent WO2005106044.                   54   0.009  1
(AL356095) Human DNA sequence from clone RP11-369J21 on chro...    54   0.009  1
(AC145402) Gorilla gorilla gorilla clone CH255-181N17, WORKI...    54   0.009  1
(AL357792) Human DNA sequence *** SEQUENCING CANCELLED *** f...    54   0.009  1
(AC012043) Homo sapiens chromosome 10 clone RP11-131C15, WOR...    54   0.009  1
(AC139722) Homo sapiens BAC clone RP11-100N20 from 4, comple...    50   0.080  4
(AC162397) Bos taurus clone CH240-120B13, WORKING DRAFT SEQU...    38   0.082  5
(GC698670) Sequence 13915 from patent US 6812339.                  50   0.087  4
(AC176352) Strongylocentrotus purpuratus clone R3-3053B20, W...    50   0.15   1
(AC015949) Homo sapiens chromosome 4 clone RP11-110P12 map 4...    50   0.15   1
(AL385453) Medicago truncatula EST MtBC28F08F1 : T3 end of c...    50   0.15   1
(AJ504348) Medicago truncatula EST, clone mtgmadc120046e02.        50   0.15   1
(AJ504225) Medicago truncatula EST, clone mtgmadc120045a08.        50   0.15   1
(AJ503988) Medicago truncatula EST, clone mtgmadc120040h06.        50   0.15   1
(AJ502580) Medicago truncatula EST, clone mtgmadc120022g02.        50   0.15   1
(AJ502273) Medicago truncatula EST, clone mtgmadc120018g06.        50   0.15   1
(AJ501069) Medicago truncatula EST, clone mtgmadc120002d09.        50   0.15   1
(BQ124772) EST610360 GLSD Medicago truncatula cDNA clone pGL...    50   0.15   1
(BI263676) NF088F11PL1F1095 Phosphate starved leaf Medicago ...    50   0.15   1
(BI262914) NF026C04PL1F1034 Phosphate starved leaf Medicago ...    50   0.15   1
(BG588555) EST490364 MHRP- Medicago truncatula cDNA clone pM...    50   0.15   1
(BF651234) NF108H09EC1F1078 Elicited cell culture Medicago t...    50   0.15   1
(BF648186) NF038E12EC1F1098 Elicited cell culture Medicago t...    50   0.15   1
(BF638975) NF063E10PL1F1081 Phosphate starved leaf Medicago ...    50   0.15   1
(BF638705) NF063H02PL1F1026 Phosphate starved leaf Medicago ...    50   0.15   1
(BF638451) NF057H07PL1F1062 Phosphate starved leaf Medicago ...    50   0.15   1
(BF638400) NF045G07PL1F1054 Phosphate starved leaf Medicago ...    50   0.15   1
(BE941652) EST421231 MGHG Medicago truncatula cDNA clone pMG...    50   0.15   1
(BE941331) EST420910 MGHG Medicago truncatula cDNA clone pMG...    50   0.15   1
(BE239608) EST403657 MHRP- Medicago truncatula cDNA clone pM...    50   0.15   1
(AW584656) N210758e MHAM Medicago truncatula/Glomus versifor...    50   0.15   1
(AW573786) EST316377 GVN Medicago truncatula cDNA clone pGVN...    50   0.15   1
(EY478749) METBH76TF JCVI-MT3 Medicago truncatula cDNA 5', m...    50   0.15   1
(AM454694) Vitis vinifera contig VV78X136186.17, whole genom...    44   0.24   4
(AZ929368) 479.dif25d11.s1 Saccharomyces kluyveri Lachancea ...    48   0.59   1
(DE288676) Bombyx mori genomic DNA, BAC clone:BES_017_J20.         48   0.59   1
(CK558890) rswpb0_000790.y1 swp Bombyx mori cDNA, mRNA seque...    48   0.59   1
(AC187371) Pan troglodytes BAC clone CH251-521J10 from chrom...    38   0.65   6
(CP001283) Bacillus cereus AH820, complete genome.                 34   0.97   2
(AE017355) Bacillus thuringiensis serovar konkukian str. 97-...    34   0.97   2
(AE017225) Bacillus anthracis str. Sterne, complete genome.        34   0.97   2
(AE017334) Bacillus anthracis str. 'Ames Ancestor', complete...    34   0.97   2
(AE016879) Bacillus anthracis str. Ames, complete genome.          34   0.97   2
(EU093669) Synthetic construct Bacillus anthracis clone FLH2...    34   1.6    2
(EK465395) 1095469420483 Global-Ocean-Sampling_GS-32-01-01-1...    36   1.7    2
(EK464286) 1095469411939 Global-Ocean-Sampling_GS-32-01-01-1...    36   1.7    2
(CU760392) A BAC library has been constructed from PN40024 g...    34   1.7    3
(AL445563) Mycoplasma pulmonis (strain UAB CTIP) complete ge...    32   2.1    11
(AC158599) Mus musculus 10 BAC RP23-122O12 (Roswell Park Can...    38   2.3    6
(X96557) P.tetraurlia epsilon-51D gene.                            46   2.3    1
(AC148536) Macaca mulatta clone CH250-68D21, WORKING DRAFT S...    46   2.3    1
(AC139119) Didelphis virginiana clone LB3-157G8, WORKING DRA...    46   2.3    1
(BH429406) BOGLO12TR BOGL Brassica oleracea genomic clone BO...    46   2.3    1
(EL662891) AGENCOURT_103353065 NICHD_XGC_tropInt_60 Xenopus ...    46   2.3    1
(DV569274) Pf_IL_289G07_TriplEx-5LD Flounder IL Induced Live...    46   2.3    1
(BI221369) 602938921F1 NCI_CGAP_Li9 Mus musculus cDNA clone ...    46   2.3    1
(AC144478) Medicago truncatula clone mth2-20b20, complete se...    42   3.0    8
(AC209103) Populus trichocarpa clone POP056-L16, complete se...    34   4.1    5
(AL445564) Mycoplasma pulmonis (strain UAB CTIP) complete ge...    38   4.7    10
(AC130747) Rattus norvegicus clone CH230-40M2, *** SEQUENCIN...    38   6.3    6
(EJ848354) 1093017867971 Global-Ocean-Sampling_GS-30-02-01-1...    34   6.4    2
(AL954843) Zebrafish DNA sequence from clone DKEY-149K8 Cont...    38   8.7    6
(BX936460) Zebrafish DNA sequence from clone CH211-69N2 in l...    44   9.1    1
(BX649457) Zebrafish DNA sequence from clone DKEYP-87E7 in l...    44   9.1    1
(AC186833) Gallus gallus BAC clone CH261-100K21 from chromos...    44   9.1    1
(BV024473) S212P60365FE6.T0 CZECHII/Ei Mus musculus STS geno...    44   9.1    1
(BX000428) Mouse DNA sequence from clone RP23-474G7 on chrom...    44   9.1    1
(AL626783) Mouse DNA sequence from clone RP23-406B13 on chro...    44   9.1    1
(AC147232) Mus musculus BAC clone RP23-311C15 from 7, comple...    44   9.1    1
(AC138678) Mus musculus chromosome 7, clone RP23-18B20, comp...    44   9.1    1
(AC128738) Mus musculus BAC clone RP24-106M4 from chromosome...    44   9.1    1
(AM481842) Vitis vinifera contig VV78X184360.5, whole genome...    44   9.1    1
(AM462900) Vitis vinifera contig VV78X056435.6, whole genome...    44   9.1    1
(AL033503) C.albicans cosmid Ca49C4.                               44   9.1    1
(AF015306) Candida albicans small GTP-binding protein SEC4p ...    44   9.1    1
(AC232554) Brassica rapa subsp. pekinensis clone KBrH013I08,...    44   9.1    1
(X96400) P.tetraurelia alpha-51D gene.                             44   9.1    1
(X85135) P.tetraurelia alpha-51D-gene for immobilization ant...    44   9.1    1
(AC121743) Rattus norvegicus clone CH230-99O14, *** SEQUENCI...    44   9.1    1
(AC111858) Rattus norvegicus clone CH230-203J17, WORKING DRA...    44   9.1    1
(AC101569) Mus musculus clone RP23-196G11, LOW-PASS SEQUENCE...    44   9.1    1
(AC092514) Papio anubis clone RP41-56L12, WORKING DRAFT SEQU...    44   9.1    1
(CU463823) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   9.1    1
(CU062654) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    44   9.1    1
(BX572088) Mouse DNA sequence *** SEQUENCING IN PROGRESS ***...    44   9.1    1
(AC233993) Gallus gallus chromosome Z clone CH261-174G3, WOR...    44   9.1    1
(EK337800) 1095467049317 Global-Ocean-Sampling_GS-31-01-01-1...    44   9.1    1
(ED408267) AUAC-aai58e05.g1 Ascaris suum whole genome shotgu...    44   9.1    1
(DX205557) OR_ABa0076E05.f OR_ABa Oryza ridleyi genomic clon...    44   9.1    1
(CU210529) Equus caballus GSS, BAC clone CH241-329H11, SP6 e...    44   9.1    1
(DW266871) UI-S-GN1-abr-k-10-0-UI.s1 UI-S-GN1 Euprymna scolo...    44   9.1    1
(BM161332) EST563855 PyBS Plasmodium yoelii yoelii cDNA clon...    44   9.1    1
(BB978665) Plasmodium berghei str. ANKA cDNA clone: OK008713...    44   9.1    1
(FK082844) XABT137849.g1 Gateway compatible cien cDNA librar...    44   9.1    1
(CP001091) Actinobacillus pleuropneumoniae serovar 7 str. AP...    44   9.1    1
(CP000687) Actinobacillus pleuropneumoniae serovar 3 str. JL...    44   9.1    1
(AE017340) Idiomarina loihiensis L2TR, complete genome.            44   9.1    1
(ED561295) MUGQ_CH252P140J12Sp6_CN140_040 CHORI-252 Vervet M...    34   10.0   3

>(C90721) Dictyostelium discoideum slug cDNA, clone SSJ869.
          Length = 587

 Score =  618 bits (312), Expect(3) = 0.0
 Identities = 336/344 (97%)
 Strand = Plus / Plus


Query: 229 gtagaagttttcgttgaacaaaatagaagtgaaacttatatttcaaactctcttgaaaaa 288
           |||||||||||||||||||||||| || ||||| ||||||||||||||||||| ||||||
Sbjct: 1   gtagaagttttcgttgaacaaaatggaggtgaaccttatatttcaaactctctggaaaaa 60


Query: 289 gtttgtgaaatcattccaagagaagattataaatcaacttgtagatcaattgttttagca 348
           |||||||||||||||||| ||||||||| |||||||||||||||||||||||||||||||
Sbjct: 61  gtttgtgaaatcattccaggagaagattttaaatcaacttgtagatcaattgttttagca 120


Query: 349 tatacaaaagatattattcaattaattattaatagagaaccatcagaaaaaatttgtcaa 408
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 tatacaaaagatattattcaattaattattaatagagaaccatcagaaaaaatttgtcaa 180


Query: 409 gagattaaagcttgtccattaccaaccccttctccatcgccatccaaacaagttaaacca 468
           |||||||||||||||||| |||||||||| ||||||||||||||||||||||||||||||
Sbjct: 181 gagattaaagcttgtccaataccaaccccatctccatcgccatccaaacaagttaaacca 240


Query: 469 aaaagaaatgaagatgatgctctacaatgtacaatttgtaaattggttgccactaaatta 528
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 aaaagaaatgaagatgatgctctacaatgtacaatttgtaaattggttgccactaaatta 300


Query: 529 gaagagtacattcaatcaaataaaaccattgaagagattgaaaa 572
           ||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 gaagagtacattcaatcaaataaaaccattgaagagattgaaaa 344

 Score =  226 bits (114), Expect(3) = 0.0
 Identities = 114/114 (100%)
 Strand = Plus / Plus


Query: 679 aaagaggatccaactcaagcttgcattaaattaaaattctgtacatcttcaagagtaact 738
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 448 aaagaggatccaactcaagcttgcattaaattaaaattctgtacatcttcaagagtaact 507


Query: 739 aaagcaattcttagtcatcatgttttgattaataagaattattaaaacattaat 792
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 508 aaagcaattcttagtcatcatgttttgattaataagaattattaaaacattaat 561

 Score =  218 bits (110), Expect(3) = 0.0
 Identities = 110/110 (100%)
 Strand = Plus / Plus


Query: 570 aaaatgaattggatgatttctgtaaaattgcttttgaaaaagatccaacccaatgccaag 629
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 341 aaaatgaattggatgatttctgtaaaattgcttttgaaaaagatccaacccaatgccaag 400


Query: 630 gttttgtacaacaatatgttccaatgattttatcttttatcaagagtaaa 679
           ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 401 gttttgtacaacaatatgttccaatgattttatcttttatcaagagtaaa 450

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 102105510
Number of Hits to DB: 1,038,080,000
Number of extensions: 64898972
Number of successful extensions: 5482471
Number of sequences better than 10.0: 112
Length of query: 894
Length of database: 101,790,757,118
Length adjustment: 24
Effective length of query: 870
Effective length of database: 99,340,224,878
Effective search space: 86425995643860
Effective search space used: 86425995643860
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.30
Homology vs Protein 
Query= Contig-U04637-1 (Contig-U04637-1Q) /CSM_Contig/Contig-U04637-1Q.Seq.d
         (894 letters)

Database: nrp_A
           3,268,448 sequences; 1,061,185,681 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC080997_1(BC080997|pid:none) Xenopus laevis MGC80725 protein, m...    64   9e-09
BC054988_1(BC054988|pid:none) Xenopus laevis hypothetical protei...    62   3e-08
AF175768_1(AF175768|pid:none) Dictyostelium discoideum SAPLIP C ...    61   6e-08
AF196308_1(AF196308|pid:none) Naegleria fowleri naegleriapore A ...    60   1e-07
U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...    60   1e-07
AF196309_1(AF196309|pid:none) Naegleria fowleri naegleriapore B ...    60   1e-07
AC116956_84(AC116956|pid:none) Dictyostelium discoideum chromoso...    57   8e-07
DQ363441_1(DQ363441|pid:none) Ictalurus punctatus clone C111BF07...    57   1e-06
BC119540_1(BC119540|pid:none) Mus musculus prosaposin-like 1, mR...    56   1e-06
(Q8C1C1) RecName: Full=Proactivator polypeptide-like 1; Contains...    56   1e-06
CR855698_1(CR855698|pid:none) Xenopus tropicalis finished cDNA, ...    56   2e-06
BT045905_1(BT045905|pid:none) Salmo salar clone ssal-rgf-536-147...    54   3e-06
AF108656_1(AF108656|pid:none) Gallus gallus prosaposin mRNA, com...    52   3e-06
(O13035) RecName: Full=Proactivator polypeptide; Contains:   Rec...    51   3e-06
BC105409_1(BC105409|pid:none) Bos taurus prosaposin, mRNA (cDNA ...    52   4e-06
CT033825_6(CT033825|pid:none) Zebrafish DNA sequence from clone ...    54   5e-06
AB170870_1(AB170870|pid:none) Macaca fascicularis brain cDNA clo...    52   7e-06
(P20097) RecName: Full=Saposin-C; AltName: Full=Co-beta-glucosid...    54   7e-06
BC155325_1(BC155325|pid:none) Danio rerio cDNA clone IMAGE:70680...    54   7e-06
AB169527_1(AB169527|pid:none) Macaca fascicularis brain cDNA, cl...    52   7e-06
AB209776_1(AB209776|pid:none) Homo sapiens mRNA for prosaposin v...    51   2e-05
AK299184_1(AK299184|pid:none) Homo sapiens cDNA FLJ54136 complet...    51   2e-05
AK223290_1(AK223290|pid:none) Homo sapiens mRNA for prosaposin (...    51   2e-05
CR861144_1(CR861144|pid:none) Pongo abelii mRNA; cDNA DKFZp459J1...    51   2e-05
M60258_1(M60258|pid:none) Human mutant cerebroside sulfate activ...    51   2e-05
(Q6IBQ6) RecName: Full=Proactivator polypeptide; Contains:   Rec...    51   2e-05
M60257_1(M60257|pid:none) Human mutant cerebroside sulfate activ...    51   2e-05
AY892719_1(AY892719|pid:none) Synthetic construct Homo sapiens c...    51   2e-05
AX704807_1(AX704807|pid:none) Sequence 39 from Patent WO03014151.      51   2e-05
CR861454_1(CR861454|pid:none) Pongo abelii mRNA; cDNA DKFZp459G1...    51   2e-05
CR388420_1(CR388420|pid:none) Zebrafish DNA sequence from clone ...    53   2e-05
BC056717_1(BC056717|pid:none) Danio rerio prosaposin, mRNA (cDNA...    53   2e-05
AF276996_1(AF276996|pid:none) Danio rerio lysosomal cofactor/neu...    53   2e-05
X57108_1(X57108|pid:none) Human gene for cerebroside sulfate act...    51   2e-05
AJ719872_1(AJ719872|pid:none) Gallus gallus mRNA for hypothetica...    52   3e-05
CR456746_1(CR456746|pid:none) Homo sapiens full open reading fra...    49   6e-05
CR859906_1(CR859906|pid:none) Pongo abelii mRNA; cDNA DKFZp468M2...    51   6e-05
BT045327_1(BT045327|pid:none) Salmo salar clone ssal-rgf-518-210...    49   7e-05
BT058972_1(BT058972|pid:none) Salmo salar clone ssal-rgf-517-167...    49   7e-05
(Q6NUJ1) RecName: Full=Proactivator polypeptide-like 1; Contains...    50   1e-04
BC068579_1(BC068579|pid:none) Homo sapiens prosaposin-like 1, mR...    50   1e-04
S21770(S21770)saposin-C - bovine                                       50   1e-04
AY815893_1(AY815893|pid:none) Schistosoma japonicum SJCHGC01869 ...    49   2e-04
BC149798_1(BC149798|pid:none) Bos taurus prosaposin-like 1, mRNA...    49   2e-04
AY811214_1(AY811214|pid:none) Schistosoma japonicum clone SJCHGC...    48   5e-04
AC115608_3(AC115608|pid:none) Dictyostelium discoideum chromosom...    48   5e-04
FN357292_36(FN357292|pid:none) Schistosoma mansoni genome sequen...    47   7e-04
AF162129_1(AF162129|pid:none) Drosophila melanogaster ecdysone d...    47   7e-04
DQ062781_1(DQ062781|pid:none) Drosophila simulans saposin-relate...    47   7e-04
AB170090_1(AB170090|pid:none) Macaca fascicularis brain cDNA clo...    47   9e-04
BC091483_1(BC091483|pid:none) Homo sapiens prosaposin, mRNA (cDN...    47   9e-04
FN357316_22(FN357316|pid:none) Schistosoma mansoni genome sequen...    47   9e-04
DQ071890_1(DQ071890|pid:none) Trichomonas vaginalis saposin-like...    47   0.001
(P10960) RecName: Full=Sulfated glycoprotein 1;          Short=S...    47   0.001
BC061759_1(BC061759|pid:none) Rattus norvegicus prosaposin, mRNA...    47   0.001
AB008449_1(AB008449|pid:none) Bombyx mori mRNA for BmP109, compl...    47   0.001
BT075327_1(BT075327|pid:none) Osmerus mordax clone omor-eva-511-...    46   0.001
AK167719_1(AK167719|pid:none) Mus musculus 15 days pregnant adul...    46   0.002
(Q61207) RecName: Full=Sulfated glycoprotein 1;          Short=S...    46   0.002
AK167073_1(AK167073|pid:none) Mus musculus blastocyst blastocyst...    46   0.002
AK159722_1(AK159722|pid:none) Mus musculus osteoclast-like cell ...    46   0.002
AK153404_1(AK153404|pid:none) Mus musculus bone marrow macrophag...    46   0.002
AK088369_1(AK088369|pid:none) Mus musculus 2 days neonate thymus...    46   0.002
AK149785_1(AK149785|pid:none) Mus musculus bone marrow macrophag...    46   0.002
AK152280_1(AK152280|pid:none) Mus musculus bone marrow macrophag...    46   0.002
AK167902_1(AK167902|pid:none) Mus musculus CRL-1722 L5178Y-R cDN...    46   0.002
AK159164_1(AK159164|pid:none) Mus musculus osteoclast-like cell ...    46   0.002
AK159634_1(AK159634|pid:none) Mus musculus osteoclast-like cell ...    46   0.002
DQ666082_1(DQ666082|pid:none) Artemia franciscana clone 13RP1.05...    46   0.002
EU441799_1(EU441799|pid:none) Xenopus borealis clone Psap.a3 pro...    45   0.003
AY811101_1(AY811101|pid:none) Schistosoma japonicum SJCHGC00199 ...    44   0.006
AB090278_1(AB090278|pid:none) Dugesia ryukyuensis Dryg mRNA for ...    44   0.007
S71616_1(S71616|pid:none) prosaposin [mice, mRNA, 2593 nt].            44   0.007
FN315321_1(FN315321|pid:none) Schistosoma japonicum isolate Anhu...    44   0.010
FN315317_1(FN315317|pid:none) Schistosoma japonicum isolate Anhu...    44   0.010
FN315319_1(FN315319|pid:none) Schistosoma japonicum isolate Anhu...    44   0.010
FN315316_1(FN315316|pid:none) Schistosoma japonicum isolate Anhu...    44   0.010
BT003256_1(BT003256|pid:none) Drosophila melanogaster LD25144 fu...    43   0.013
FN315781_1(FN315781|pid:none) Schistosoma japonicum isolate Anhu...    43   0.013
BX537304_6(BX537304|pid:none) Zebrafish DNA sequence from clone ...    43   0.013
U57999_1(U57999|pid:none) Mus musculus prosaposin (psap\SGP-1) g...    43   0.013
AY223355_1(AY223355|pid:none) Schistosoma japonicum SJCHGC01899 ...    43   0.013
FN315770_1(FN315770|pid:none) Schistosoma japonicum isolate Anhu...    43   0.013
X57107_1(X57107|pid:none) Human gene for cerebroside sulfate act...    43   0.016
FN315783_1(FN315783|pid:none) Schistosoma japonicum isolate Anhu...    43   0.016
FN315780_1(FN315780|pid:none) Schistosoma japonicum isolate Anhu...    43   0.016
FN315782_1(FN315782|pid:none) Schistosoma japonicum isolate Anhu...    42   0.037
FN315771_1(FN315771|pid:none) Schistosoma japonicum isolate Anhu...    42   0.037
EZ000561_1(EZ000561|pid:none) TSA: Culex tarsalis Ctar-515 sapos...    41   0.063
AY222887_1(AY222887|pid:none) Schistosoma japonicum clone ZZZ290...    40   0.14
FN315614_1(FN315614|pid:none) Schistosoma japonicum isolate Anhu...    40   0.14
FN315627_1(FN315627|pid:none) Schistosoma japonicum isolate Anhu...    40   0.14
AY222889_1(AY222889|pid:none) Schistosoma japonicum clone ZZZ315...    40   0.14
S47096(S47096)cynarase (EC 3.4.23.-) - cardoon                         39   0.18
T12049(T12049) cyprosin (EC 3.4.23.-) - cardoon (fragment)  &X69...    39   0.18
AY813311_1(AY813311|pid:none) Schistosoma japonicum SJCHGC02291 ...    39   0.18
AY811092_1(AY811092|pid:none) Schistosoma japonicum clone SJCHGC...    39   0.24
AY811061_1(AY811061|pid:none) Schistosoma japonicum clone SJCHGC...    39   0.24
AY811148_1(AY811148|pid:none) Schistosoma japonicum clone SJCHGC...    39   0.24
AY811208_1(AY811208|pid:none) Schistosoma japonicum clone SJCHGC...    38   0.41
(P22355) RecName: Full=Pulmonary surfactant-associated protein B...    38   0.41
BC072466_1(BC072466|pid:none) Rattus norvegicus surfactant prote...    38   0.41
FN315623_1(FN315623|pid:none) Schistosoma japonicum isolate Anhu...    37   0.69
FN315620_1(FN315620|pid:none) Schistosoma japonicum isolate Anhu...    37   0.69
FN315625_1(FN315625|pid:none) Schistosoma japonicum isolate Anhu...    37   0.69
FN315621_1(FN315621|pid:none) Schistosoma japonicum isolate Anhu...    37   0.69
AJ237674_1(AJ237674|pid:none) Cynara cardunculus mRNA for prepro...    37   0.69
FN315624_1(FN315624|pid:none) Schistosoma japonicum isolate Anhu...    37   0.91
A27562(A27562) T-cell activation protein, splice form 519 - huma...    37   1.2
AC117075_38(AC117075|pid:none) Dictyostelium discoideum chromoso...    37   1.2
A00142_1(A00142|pid:none) H.sapiens LAG-2 gene encoding lymphoki...    37   1.2
(P40782) RecName: Full=Cyprosin;          EC=3.4.23.-; Flags: Pr...    36   1.5
D12777_1(D12777|pid:none) Oryza sativa Japonica Group mRNA for a...    36   2.0
(P42211) RecName: Full=Aspartic proteinase;          EC=3.4.23.-...    36   2.0
(Q29075) RecName: Full=Antimicrobial peptide NK-lysin;          ...    35   3.4
(O18823) RecName: Full=Acyloxyacyl hydrolase;          EC=3.1.1....    35   3.4
AB045892_1(AB045892|pid:none) Nepenthes alata NaAP2 mRNA for asp...    35   3.4
(P22749) RecName: Full=Granulysin; AltName: Full=Protein NKG5; A...    35   4.5
AJ132884_1(AJ132884|pid:none) Cynara cardunculus mRNA for prepro...    35   4.5
AK304728_1(AK304728|pid:none) Homo sapiens cDNA FLJ51776 complet...    35   4.5
T27869(T27869)sphingomyelin phosphodiesterase (EC 3.1.4.12), aci...    34   5.9
(Q23498) RecName: Full=Sphingomyelin phosphodiesterase 2;       ...    34   5.9
AY815320_1(AY815320|pid:none) Schistosoma japonicum clone SJCHGC...    34   5.9
(Q91G49) RecName: Full=Uncharacterized protein 050L;  &AF303741_...    34   7.6
FN315905_1(FN315905|pid:none) Schistosoma japonicum isolate Anhu...    34   7.7
Y09123_1(Y09123|pid:none) C.calcitrapa mRNA for aspartic protein...    34   7.7
FN315903_1(FN315903|pid:none) Schistosoma japonicum isolate Anhu...    34   7.7

>BC080997_1(BC080997|pid:none) Xenopus laevis MGC80725 protein, mRNA
           (cDNA clone MGC:80725 IMAGE:5511990), complete cds.
          Length = 518

 Score = 63.5 bits (153), Expect = 9e-09
 Identities = 48/200 (24%), Positives = 88/200 (44%), Gaps = 7/200 (3%)
 Frame = +1

Query: 157 ISGNSIVSSNEVAQCDVCNYLVTMVEVFVEQNRSETYISNSLEKVCEIIPREDYKSTCRS 336
           I+ N +  +N +  C+VC  +V+ +E  ++ NR+   I + LEKVC+++P + Y   C
Sbjct: 297 ITKNPLPGNNVL--CEVCELMVSQLEKLLDNNRTRENIKHGLEKVCKLLPSQ-YTQKCED 353

Query: 337 IVLAYTKDIIQLIINREPSEKICQEIKACPLXXXXXXXXXXXXXXRNEDDALQCTICKLV 516
           ++  Y+  +I+L+      + IC  +  C                   D    C +CK++
Sbjct: 354 MIEEYSDALIELLEQEANPQAICTALGYCSGSKNLKIVKISAEKAAAGD---YCAVCKML 410

Query: 517 ATKLEEYIQSNKTXXXXXXXXG*FL*NCF*KRSNPMPRFCTTICS---NDF--IFYQE*K 681
              ++E ++ N T        G        +  N +P      CS   N++  +F Q
Sbjct: 411 MRYVDELLEKNATEIRIKAFLG--------RICNFLPDSMQNECSALVNEYEPLFIQLLL 462

Query: 682 E--DPTQACIKLKFCTSSRV 735
           E  DP+  CIK+  C + +V
Sbjct: 463 EALDPSFICIKVNLCQNKKV 482

 Score = 41.6 bits (96), Expect = 0.037
 Identities = 19/59 (32%), Positives = 32/59 (54%)
 Frame = +1

Query: 199 CDVCNYLVTMVEVFVEQNRSETYISNSLEKVCEIIPREDYKSTCRSIVLAYTKDIIQLI 375
           CD C  +VT++  +++ N ++  I   L KVC+ IP     STC+  V  Y   ++ L+
Sbjct: 63  CDFCKEVVTVLGNYLKDNITQDEIKQYLNKVCDFIPDPGLASTCKQEVSDYFTIVLNLL 121

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 1,027,321,772
Number of extensions: 17694021
Number of successful extensions: 43534
Number of sequences better than 10.0: 127
Number of HSP's gapped: 43427
Number of HSP's successfully gapped: 233
Length of query: 298
Length of database: 1,061,185,681
Length adjustment: 128
Effective length of query: 170
Effective length of database: 642,824,337
Effective search space: 109280137290
Effective search space used: 109280137290
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 32 (16.9 bits)
PSORT 

 psg: 0.35  gvh: 0.61  alm: 0.18  top: -0.13  tms: 0.07  mit: 0.17  mip: 0.00
 nuc: 0.24  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 20.0 %: nuclear
	 12.0 %: Golgi
	 12.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for Contig-U04637-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 0
SS (DIR, S) 8
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0