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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
Contig-U04220-1
Contig ID Contig-U04220-1
Contig update 2001. 8.29
Contig sequence 
>Contig-U04220-1 (Contig-U04220-1Q) /CSM_Contig/Contig-U04220-1Q.Seq.d
CACATAATTAAAAATGGAGTTACATGTTCAATTAAAACATAGTACTGACT
GTTTCGTTTCACTTCCACCTAAAATAGTACATTCACTGTTATTATTATCA
GAAAAACAATCAAAATCATTAGGTACATTAGGATTAGAAATAACATGGTA
TGATAAAATTAATAAAAAAGAGAATAAAGGATATGTTGGATGGGCAGGTG
GATCAACAGATCCAAGATTCACAGATTCAATAGAGATGAGTCAAGAGATG
GCACAATGTTTAGGTGGTATAAAGAATGAGCAAAAATTAAAACTAAAAGC
ATTAAATAATATTGAATTAGCTCATTCAGTTCAAGTTGAACCATTAACAT
CAGATGATTGGGAAATTATGGAAGTTCATCAACAATACCTAGAAGAACAA
TTACTTAATCAAGTTAATATTTTATATAGTGGTCAAATCGTACCAATTTG
GATTCATCATAAAACTATAATTAAATTAAAAGTTACTGAAACTTTACCAA
CACCAGTAGTTAAATTATCTTCAAATTCTGAAATTATAGTTGCACCAAAA
CCTAGAAATTTACCAACAACAACAACATCATCACAACAACAACAAATTTC
AAAAGAAACTTTAAAACCAAGATTTTTACAAATTAAAGATTTTAAAATAG
ATTATAATAATAATAATACATTTATAAATGAAATTTATATAAATAAAGAG
TTATTAAACCAATTTCAATGGAATATTGGTGATATTATAGAAATTTCAAA
AGTTTCAAAAAATAATAATAAAAATAT
Gap no gap
Contig length 777
Chromosome number (1..6, M) 5
Chromosome length 5062330
Start point 3303783
End point 3304561
Strand (PLUS/MINUS) PLUS
Number of clones 1
Number of EST 1
Link to clone list U04220
List of clone(s) 

est1=SLI309E,1,778
Translated Amino Acid sequence 
t*LKMELHVQLKHSTDCFVSLPPKIVHSLLLLSEKQSKSLGTLGLEITWYDKINKKENKG
YVGWAGGSTDPRFTDSIEMSQEMAQCLGGIKNEQKLKLKALNNIELAHSVQVEPLTSDDW
EIMEVHQQYLEEQLLNQVNILYSGQIVPIWIHHKTIIKLKVTETLPTPVVKLSSNSEIIV
APKPRNLPTTTTSSQQQQISKETLKPRFLQIKDFKIDYNNNNTFINEIYINKELLNQFQW
NIGDIIEISKVSKNNNKN
Translated Amino Acid sequence (All Frames) 
Frame A:
hiikngvtcsikt*y*lfrftst*nstftviiirktikiiryirirnnmv**n**kre*r
icwmgrwinrskihrfnrdesrdgtmfrwyke*akiktksik*y*issfsss*tinir*l
gnygssstiprrtit*ss*yfi*wsnrtnldss*nyn*iksy*nftntss*iifkf*nys
ctkt*kftnnnniittttnfkrnfktkiftn*rf*nrl****yiyk*nlyk*rvikpism
eyw*yyrnfksfkk***ky


Frame B:
t*LKMELHVQLKHSTDCFVSLPPKIVHSLLLLSEKQSKSLGTLGLEITWYDKINKKENKG
YVGWAGGSTDPRFTDSIEMSQEMAQCLGGIKNEQKLKLKALNNIELAHSVQVEPLTSDDW
EIMEVHQQYLEEQLLNQVNILYSGQIVPIWIHHKTIIKLKVTETLPTPVVKLSSNSEIIV
APKPRNLPTTTTSSQQQQISKETLKPRFLQIKDFKIDYNNNNTFINEIYINKELLNQFQW
NIGDIIEISKVSKNNNKN


Frame C:
hn*kwsymfn*nivltvsfhfhlk*yihcyyyqknnqnh*vh*d*k*hgmiklikkrikd
mldgqvdqqiqdsqiq*r*vkrwhnv*vv*rmskn*n*kh*iiln*liqfklnh*hqmig
klwkfinnt*knnyliklifyivvksyqfgfiikl*ln*kllklyqhq*lnylqilkl*l
hqnleiyqqqqhhhnnnkfqkkl*nqdfyklkilk*iiiiiihl*mkfi*iksy*tnfng
ilvil*kfqkfqkiiiki
own update 2004. 6. 9
Homology vs CSM-cDNA 
Query= Contig-U04220-1 (Contig-U04220-1Q)
/CSM_Contig/Contig-U04220-1Q.Seq.d
         (777 letters)

Database: CSM
           6905 sequences; 5,674,871 total letters


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

Contig-U04220-1 (Contig-U04220-1Q) /CSM_Contig/Conti...  1116  0.0
Contig-U12540-1 (Contig-U12540-1Q) /CSM_Contig/Conti...    38  0.014
Contig-U12916-1 (Contig-U12916-1Q) /CSM_Contig/Conti...    36  0.055
Contig-U10555-1 (Contig-U10555-1Q) /CSM_Contig/Conti...    36  0.055
Contig-U09824-1 (Contig-U09824-1Q) /CSM_Contig/Conti...    36  0.055
Contig-U01359-1 (Contig-U01359-1Q) /CSM_Contig/Conti...    36  0.055
Contig-U14414-1 (Contig-U14414-1Q) /CSM_Contig/Conti...    34  0.22
Contig-U12039-1 (Contig-U12039-1Q) /CSM_Contig/Conti...    34  0.22
Contig-U11850-1 (Contig-U11850-1Q) /CSM_Contig/Conti...    34  0.22
Contig-U10454-1 (Contig-U10454-1Q) /CSM_Contig/Conti...    34  0.22

>Contig-U04220-1 (Contig-U04220-1Q) /CSM_Contig/Contig-U04220-1Q.Seq.d
          Length = 777

 Score = 1116 bits (563), Expect = 0.0
 Identities = 563/563 (100%)
 Strand = Plus / Plus

                                                                       
Query: 1   cacataattaaaaatggagttacatgttcaattaaaacatagtactgactgtttcgtttc 60
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   cacataattaaaaatggagttacatgttcaattaaaacatagtactgactgtttcgtttc 60

                                                                       
Query: 61  acttccacctaaaatagtacattcactgttattattatcagaaaaacaatcaaaatcatt 120
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  acttccacctaaaatagtacattcactgttattattatcagaaaaacaatcaaaatcatt 120

                                                                       
Query: 121 aggtacattaggattagaaataacatggtatgataaaattaataaaaaagagaataaagg 180
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 aggtacattaggattagaaataacatggtatgataaaattaataaaaaagagaataaagg 180

                                                                       
Query: 181 atatgttggatgggcaggtggatcaacagatccaagattcacagattcaatagagatgag 240
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 atatgttggatgggcaggtggatcaacagatccaagattcacagattcaatagagatgag 240

                                                                       
Query: 241 tcaagagatggcacaatgtttaggtggtataaagaatgagcaaaaattaaaactaaaagc 300
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 tcaagagatggcacaatgtttaggtggtataaagaatgagcaaaaattaaaactaaaagc 300

                                                                       
Query: 301 attaaataatattgaattagctcattcagttcaagttgaaccattaacatcagatgattg 360
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 301 attaaataatattgaattagctcattcagttcaagttgaaccattaacatcagatgattg 360

                                                                       
Query: 361 ggaaattatggaagttcatcaacaatacctagaagaacaattacttaatcaagttaatat 420
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 361 ggaaattatggaagttcatcaacaatacctagaagaacaattacttaatcaagttaatat 420

                                                                       
Query: 421 tttatatagtggtcaaatcgtaccaatttggattcatcataaaactataattaaattaaa 480
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 421 tttatatagtggtcaaatcgtaccaatttggattcatcataaaactataattaaattaaa 480

                                                                       
Query: 481 agttactgaaactttaccaacaccagtagttaaattatcttcaaattctgaaattatagt 540
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 481 agttactgaaactttaccaacaccagtagttaaattatcttcaaattctgaaattatagt 540

                                  
Query: 541 tgcaccaaaacctagaaatttac 563
           |||||||||||||||||||||||
Sbjct: 541 tgcaccaaaacctagaaatttac 563


 Score =  119 bits (60), Expect = 5e-27
 Identities = 60/60 (100%)
 Strand = Plus / Plus

                                                                       
Query: 697 agagttattaaaccaatttcaatggaatattggtgatattatagaaatttcaaaagtttc 756
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 697 agagttattaaaccaatttcaatggaatattggtgatattatagaaatttcaaaagtttc 756


 Score = 89.7 bits (45), Expect = 4e-18
 Identities = 45/45 (100%)
 Strand = Plus / Plus

                                                        
Query: 596 atttcaaaagaaactttaaaaccaagatttttacaaattaaagat 640
           |||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 596 atttcaaaagaaactttaaaaccaagatttttacaaattaaagat 640


>Contig-U12540-1 (Contig-U12540-1Q) /CSM_Contig/Contig-U12540-1Q.Seq.d
          Length = 1281

 Score = 38.2 bits (19), Expect = 0.014
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 596 atttcaaaagaaactttaa 614
           |||||||||||||||||||
Sbjct: 872 atttcaaaagaaactttaa 890


>Contig-U12916-1 (Contig-U12916-1Q) /CSM_Contig/Contig-U12916-1Q.Seq.d
          Length = 2025

 Score = 36.2 bits (18), Expect = 0.055
 Identities = 18/18 (100%)
 Strand = Plus / Plus

                              
Query: 342  cattaacatcagatgatt 359
            ||||||||||||||||||
Sbjct: 1532 cattaacatcagatgatt 1549


  Database: CSM
    Posted date:  Jun 9, 2004  7:35 PM
  Number of letters in database: 5,674,871
  Number of sequences in database:  6905
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 15,373
Number of Sequences: 6905
Number of extensions: 15373
Number of successful extensions: 1758
Number of sequences better than 10.0: 200
length of query: 777
length of database: 5,674,871
effective HSP length: 16
effective length of query: 761
effective length of database: 5,564,391
effective search space: 4234501551
effective search space used: 4234501551
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 15 (30.2 bits)
dna update 2009. 6.14
Homology vs DNA 
Query= Contig-U04220-1 (Contig-U04220-1Q) /CSM_Contig/Contig-U04220-1Q.Seq.d
         (777 letters)

Database: ddbj_A
           102,105,510 sequences; 101,790,757,118 total letters

Searching..................................................done

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU053281) Dictyostelium discoideum slug cDNA, clone SLI309.      672   0.0    2
(AU062201) Dictyostelium discoideum slug cDNA, clone SLI309.      654   0.0    1
(AM464956) Vitis vinifera contig VV78X222996.6, whole genome...    42   0.001  3
(CN577812) rf47g07.x1 Meloidogyne hapla J2 pAMP1 v1 Meloidog...    54   0.008  1
(EB901157) 10077492 ZF28 Danio rerio cDNA clone 3317317, mRN...    40   0.027  2
(EK428550) 1095516061455 Global-Ocean-Sampling_GS-31-01-01-1...    34   0.099  2
(AC215450) Solanum lycopersicum chromosome 2 clone C02SLe002...    40   0.12   3
(FI115246) MUGQ_CH252P486D05T7_CH0181_029 CHORI-252 Vervet M...    50   0.13   1
(CG389063) ZMMBBc0570H17r ZMMBBc Zea mays subsp. mays genomi...    46   0.32   2
(AC116977) Dictyostelium discoideum chromosome 2 map 5515173...    38   0.37   11
(AC118653) Homo sapiens chromosome 17, clone CTD-2319F6, com...    48   0.44   3
(AC021513) Homo sapiens chromosome 17, clone RP11-281G10, co...    48   0.44   4
(AM285318) Spiroplasma citri GII3-3X chromosome, contig Cont...    34   0.46   6
(AC006079) Homo sapiens chromosome 17, clone hRPK.855_D_21, ...    48   0.46   4
(AE014828) Plasmodium falciparum 3D7 chromosome 14 section 1...    48   0.51   1
(AC006448) Homo sapiens chromosome 17, clone RP11-423M11, co...    48   0.51   1
(AC133046) Rattus norvegicus clone CH230-127N11, *** SEQUENC...    48   0.51   1
(AC098498) Rattus norvegicus clone CH230-34J21, WORKING DRAF...    48   0.51   1
(AC084836) Homo sapiens clone RP11-58N12, WORKING DRAFT SEQU...    48   0.51   1
(ER372168) 1093025094113 Global-Ocean-Sampling_GS-34-01-01-1...    48   0.51   1
(EW996378) LJGUS_P01_F05 Adult female Lutzomyia longipalpis ...    48   0.51   1
(AC146863) Medicago truncatula chromosome 6 clone mth2-175h2...    42   0.54   3
(CR932985) Zebrafish DNA sequence from clone DKEY-169L5 in l...    38   0.75   5
(AC079163) Homo sapiens BAC clone RP11-707K3 from 2, complet...    34   0.83   9
(CS468878) Sequence 69 from Patent EP1748080.                      36   0.83   9
(CS468877) Sequence 68 from Patent EP1748080.                      36   0.83   9
(CS419355) Sequence 69 from Patent WO2006094836.                   36   0.83   9
(CS419354) Sequence 68 from Patent WO2006094836.                   36   0.83   9
(AX458518) Sequence 64 from Patent WO0246454.                      34   0.86   6
(AL929359) Plasmodium falciparum strain 3D7, chromosome 9; s...    34   1.6    8
(AX344556) Sequence 7 from Patent WO0200932.                       34   1.7    8
(AC026699) Homo sapiens chromosome 5 clone CTC-489F24, WORKI...    36   1.9    6
(BC144788) Mus musculus cDNA clone IMAGE:9053336.                  46   2.0    1
(BC137621) Mus musculus RIKEN cDNA 6030446N20 gene, mRNA (cD...    46   2.0    1
(BC094382) Mus musculus RIKEN cDNA 6030446N20 gene, mRNA (cD...    46   2.0    1
(AC132941) Mus musculus chromosome 18, clone RP23-163E22, co...    46   2.0    1
(AC150412) Branchiostoma floridae clone CH302-60C21, complet...    46   2.0    1
(AC122698) Homo sapiens chromosome 5 clone CTD-2313P7, compl...    46   2.0    1
(AC118863) Rattus norvegicus clone CH230-451A21, *** SEQUENC...    46   2.0    1
(AC102265) Mus musculus chromosome 18 clone RP24-244L12 map ...    46   2.0    1
(CU571112) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    46   2.0    1
(AC189205) Brassica rapa subsp. pekinensis clone KBrB006O19,...    46   2.0    1
(AK038472) Mus musculus adult male hypothalamus cDNA, RIKEN ...    46   2.0    1
(AK031517) Mus musculus 13 days embryo male testis cDNA, RIK...    46   2.0    1
(FI075257) CHO_OF7352xe21f1.ab1 CHO_OF7 Nicotiana tabacum ge...    46   2.0    1
(DX573624) Phaseolus-Bat-7_01_C13.F Phaseolus-Bat-7 Phaseolu...    46   2.0    1
(DY448380) HSAA-aaa103l09.g2 UCI-11 Hydra vulgaris cDNA 5', ...    46   2.0    1
(CN461090) UI-M-HB0-cpa-b-14-0-UI.r1 NIH_BMAP_HB0 Mus muscul...    46   2.0    1
(CK352362) AGENCOURT_17057883 NIH_ZGC_9 Danio rerio cDNA clo...    46   2.0    1
(BI788704) ie42g06.x1 Kaestner ngn3 wt Mus musculus cDNA 3',...    46   2.0    1
(BI080602) 602877092F1 NCI_CGAP_Mam2 Mus musculus cDNA clone...    46   2.0    1
(DQ302101) Primeuchroeus sp. M48 12S ribosomal RNA, tRNA-Val...    38   2.0    3
(AG279927) Mus musculus molossinus DNA, clone:MSMg01-051A12....    40   2.7    2
(AY376688) Crinipellis perniciosa mitochondrion, complete ge...    36   2.8    5
(AP003515) Clostridium perfringens str. 13 plasmid pCP13 DNA...    38   2.8    4
(EJ344394) 1092963537426 Global-Ocean-Sampling_GS-28-01-01-1...    34   2.9    3
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...    34   3.0    10
(CP000123) Mycoplasma capricolum subsp. capricolum ATCC 2734...    34   3.2    15
(AC225315) Nomascus leucogenys chromosome UNK clone CH271-59...    42   3.8    6
(AP001910) Homo sapiens genomic DNA, chromosome 18q21 clone:...    40   4.2    5
(EH216154) USDA-FP_178958 WHMH (pink hibiscus mealybug) Maco...    32   4.4    3
(ER300827) 1092343681248 Global-Ocean-Sampling_GS-34-01-01-1...    36   4.6    2
(AE017332) Mycoplasma hyopneumoniae 232, complete genome.          36   4.8    11
(EK255020) 1095462006566 Global-Ocean-Sampling_GS-31-01-01-1...    34   4.9    2
(DE144747) Bombyx mori genomic DNA, Fosmid clone:RO0283-B06_R.     34   4.9    3
(EH212794) USDA-FP_175579 WHMH (pink hibiscus mealybug) Maco...    32   5.1    3
(CT333686) Sus scrofa genomic clone CH242-389N18, genomic su...    34   5.1    2
(BZ506145) BOOAZ83TF BO_1.6_2_KB_tot Brassica oleracea genom...    34   5.3    2
(BD061520) Genome DNA of symbiotic bacteria of aphid.              32   5.3    13
(AR409405) Sequence 1 from patent US 6632935.                      32   5.3    13
(BA000003) Buchnera aphidicola str. APS (Acyrthosiphon pisum...    32   5.3    13
(CP001158) Buchnera aphidicola str. Tuc7 (Acyrthosiphon pisu...    32   5.4    13
(CP001161) Buchnera aphidicola str. 5A (Acyrthosiphon pisum)...    32   5.4    13
(AL929357) Plasmodium falciparum strain 3D7, chromosome 9; s...    36   5.8    9
(AY700145) Polysphondylium pallidum mitochondrion DNA, compl...    34   6.7    4
(AC025473) Homo sapiens chromosome 5 clone RP11-183N2, WORKI...    36   7.0    4
(AM285307) Spiroplasma citri GII3-3X chromosome, contig Cont...    32   7.4    6
(AE014842) Plasmodium falciparum 3D7 chromosome 11 section 7...    38   7.6    8
(CU464122) Zebrafish DNA sequence from clone CH73-54C11 in l...    44   7.9    1
(CT573440) Zebrafish DNA sequence from clone CH211-246E12 in...    44   7.9    1
(BV857638) pRRL2:Newb:20080411:232205 USMARC Pig Breed SNP D...    44   7.9    1
(AC184055) Pan troglodytes BAC clone CH251-635D2 from chromo...    44   7.9    1
(AL132956) Arabidopsis thaliana DNA chromosome 3, BAC clone ...    44   7.9    1
(AC012329) Arabidopsis thaliana chromosome 1 BAC T1G12 genom...    44   7.9    1
(EA102648) Sequence 31 from patent US 7195870.                     44   7.9    1
(CQ749738) Sequence 35672 from Patent WO02068579.                  44   7.9    1
(AX345536) Sequence 607 from Patent WO0200928.                     44   7.9    1
(AX277868) Sequence 31 from Patent WO0177375.                      44   7.9    1
(EU427336) Geocoris pallidipennis mitochondrion, complete ge...    44   7.9    1
(AP009033) Bombyx mori genomic DNA, chromosome 15, BAC clone...    44   7.9    1
(AE014297) Drosophila melanogaster chromosome 3R, complete s...    44   7.9    1
(AC009457) Drosophila melanogaster, chromosome 3R, region 88...    44   7.9    1
(AL160257) Human DNA sequence from clone RP11-77O2 on chromo...    44   7.9    1
(AL117376) Human DNA sequence from clone RP5-971B4 on chromo...    44   7.9    1
(AL031771) Human DNA sequence from clone RP1-217O16 on chrom...    44   7.9    1
(AF490843) Homo sapiens clone PAC 70L19, HOXC gene cluster.        44   7.9    1
(AC023794) Homo sapiens 12 BAC RP11-834C11 (Roswell Park Can...    44   7.9    1
(AC132966) Rattus norvegicus clone CH230-210B24, WORKING DRA...    44   7.9    1
(AC129745) Rattus norvegicus clone CH230-175A22, WORKING DRA...    44   7.9    1
(AC128348) Rattus norvegicus clone CH230-129P3, WORKING DRAF...    44   7.9    1
(AC103263) Rattus norvegicus clone CH230-169O3, *** SEQUENCI...    44   7.9    1
(AC216654) Solanum lycopersicum chromosome 6 clone C06HBa022...    44   7.9    1
(AC211411) Zea mays chromosome 5 clone CH201-339E7; ZMMBBc03...    44   7.9    1
(AC209616) Spermophilus tridecemlineatus clone VMRC20-211C4,...    44   7.9    1
(AC017290) Drosophila melanogaster, *** SEQUENCING IN PROGRE...    44   7.9    1
(ER773706) TAACSP3AShA_0065E07R TAACSP3AShA Triticum aestivu...    44   7.9    1
(ER393790) 1095339007768 Global-Ocean-Sampling_GS-34-01-01-1...    44   7.9    1
(EK183796) 1095458135020 Global-Ocean-Sampling_GS-31-01-01-1...    44   7.9    1
(EJ368620) 1092963715047 Global-Ocean-Sampling_GS-28-01-01-1...    44   7.9    1
(ED454216) AUAC-aar88h10.b1 Ascaris suum whole genome shotgu...    44   7.9    1
(DU693716) Bst026246 lambda03 Boechera stricta genomic clone...    44   7.9    1
(DU690103) Bst022633 lambda03 Boechera stricta genomic clone...    44   7.9    1
(DE442725) Bombyx mori genomic DNA, Fosmid clone:GBMFno32_b0...    44   7.9    1
(DE442719) Bombyx mori genomic DNA, Fosmid clone:GBMFno32_b0...    44   7.9    1
(CE495561) tigr-gss-dog-17000310274352 Dog Library Canis lup...    44   7.9    1
(BZ215606) CH230-416P18.TV CHORI-230 Segment 2 Rattus norveg...    44   7.9    1
(CT811045) Paramecium tetraurelia 5-PRIME EST from clone LK0...    44   7.9    1
(CT808332) Paramecium tetraurelia 5-PRIME EST from clone LK0...    44   7.9    1
(CT775962) Paramecium tetraurelia 5-PRIME EST from clone LK0...    44   7.9    1
(CT758968) Paramecium tetraurelia 5-PRIME EST from clone LK0...    44   7.9    1
(CT756424) Paramecium tetraurelia 5-PRIME EST from clone LK0...    44   7.9    1
(CT743532) Paramecium tetraurelia 5-PRIME EST from clone LK0...    44   7.9    1
(CT743207) Paramecium tetraurelia 5-PRIME EST from clone LK0...    44   7.9    1
(CD309062) StrPu691.011429 Sea urchin larva cDNA library MPM...    44   7.9    1
(BF879723) RC2-ET0188-271100-012-f04 ET0188 Homo sapiens cDN...    44   7.9    1
(BE033585) MF04B03 MF Mesembryanthemum crystallinum cDNA 5' ...    44   7.9    1
(AW827299) xn11g08.y1 NCI_CGAP_Li5 Homo sapiens cDNA clone I...    44   7.9    1
(EV837981) FMMA083TF Tetrahymena thermophila SB210 cDNA libr...    44   7.9    1
(EV598103) Dmel_neb_014899_0827_2082 D. melanogaster - 454 s...    44   7.9    1
(CP000361) Arcobacter butzleri RM4018, complete genome.            44   7.9    1
(AC116956) Dictyostelium discoideum chromosome 2 map 1418423...    34   8.3    11
(AM441922) Vitis vinifera, whole genome shotgun sequence, co...    40   8.3    3
(CU984549) Brassica rapa subsp. pekinensis clone KBrB040I06,...    34   8.3    5
(AC091067) Homo sapiens chromosome 18, clone RP11-702M18, co...    38   8.7    7
(CP001056) Clostridium botulinum B str. Eklund 17B, complete...    34   8.7    17
(AP010940) Solanum lycopersicum DNA, chromosome 8, clone: C0...    38   8.9    4
(FH510702) CHO_OF4704xh08f1.ab1 CHO_OF4 Nicotiana tabacum ge...    40   9.2    2
(AC098595) Homo sapiens BAC clone RP11-495L18 from 4, comple...    40   9.3    5
(AB232978) Mecysmoderes nigrinus mitochondrial gene for 12S ...    34   9.4    2

>(AU053281) Dictyostelium discoideum slug cDNA, clone SLI309.
          Length = 554

 Score =  672 bits (339), Expect(2) = 0.0
 Identities = 339/339 (100%)
 Strand = Plus / Plus


Query: 225 attcaatagagatgagtcaagagatggcacaatgtttaggtggtataaagaatgagcaaa 284
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1   attcaatagagatgagtcaagagatggcacaatgtttaggtggtataaagaatgagcaaa 60


Query: 285 aattaaaactaaaagcattaaataatattgaattagctcattcagttcaagttgaaccat 344
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61  aattaaaactaaaagcattaaataatattgaattagctcattcagttcaagttgaaccat 120


Query: 345 taacatcagatgattgggaaattatggaagttcatcaacaatacctagaagaacaattac 404
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 121 taacatcagatgattgggaaattatggaagttcatcaacaatacctagaagaacaattac 180


Query: 405 ttaatcaagttaatattttatatagtggtcaaatcgtaccaatttggattcatcataaaa 464
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 ttaatcaagttaatattttatatagtggtcaaatcgtaccaatttggattcatcataaaa 240


Query: 465 ctataattaaattaaaagttactgaaactttaccaacaccagtagttaaattatcttcaa 524
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 ctataattaaattaaaagttactgaaactttaccaacaccagtagttaaattatcttcaa 300


Query: 525 attctgaaattatagttgcaccaaaacctagaaatttac 563
           |||||||||||||||||||||||||||||||||||||||
Sbjct: 301 attctgaaattatagttgcaccaaaacctagaaatttac 339

 Score = 95.6 bits (48), Expect(2) = 0.0
 Identities = 48/48 (100%)
 Strand = Plus / Plus


Query: 709 ccaatttcaatggaatattggtgatattatagaaatttcaaaagtttc 756
           ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 485 ccaatttcaatggaatattggtgatattatagaaatttcaaaagtttc 532

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 102105510
Number of Hits to DB: 881,722,564
Number of extensions: 59288155
Number of successful extensions: 5487130
Number of sequences better than 10.0: 143
Length of query: 777
Length of database: 101,790,757,118
Length adjustment: 24
Effective length of query: 753
Effective length of database: 99,340,224,878
Effective search space: 74803189333134
Effective search space used: 74803189333134
X1: 11 (21.8 bits)
S2: 22 (44.1 bits)
protein update 2009. 7.29
Homology vs Protein 
Query= Contig-U04220-1 (Contig-U04220-1Q) /CSM_Contig/Contig-U04220-1Q.Seq.d
         (777 letters)

Database: nrp_A
           3,268,448 sequences; 1,061,185,681 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54GX5) RecName: Full=Peroxisome biogenesis factor 1; AltName: ...   462   e-129
AF275382_1(AF275382|pid:none) Arabidopsis thaliana peroxisome bi...    92   2e-17
AB006697_3(AB006697|pid:none) Arabidopsis thaliana genomic DNA, ...    92   2e-17
(Q5BL07) RecName: Full=Peroxisome biogenesis factor 1; AltName: ...    86   2e-15
BC118794_1(BC118794|pid:none) Xenopus tropicalis hypothetical pr...    85   3e-15
AE017344_617(AE017344|pid:none) Cryptococcus neoformans var. neo...    83   9e-15
(O43933) RecName: Full=Peroxisome biogenesis factor 1; AltName: ...    83   9e-15
AP003928_11(AP003928|pid:none) Oryza sativa Japonica Group genom...    81   4e-14
AP008214_1726(AP008214|pid:none) Oryza sativa (japonica cultivar...    81   4e-14
BC072751_1(BC072751|pid:none) Xenopus laevis MGC79116 protein, m...    79   1e-13
CP000499_650(CP000499|pid:none) Pichia stipitis CBS 6054 chromos...    74   5e-12
CR382138_940(CR382138|pid:none) Debaryomyces hansenii strain CBS...    73   1e-11
FN392321_1074(FN392321|pid:none) Pichia pastoris GS115 chromosom...    72   2e-11
AP006852_232(AP006852|pid:none) Candida albicans genomic DNA, ch...    71   4e-11
AF208231_1(AF208231|pid:none) Yarrowia lipolytica peroxisome ass...    70   6e-11
CR382129_577(CR382129|pid:none) Yarrowia lipolytica strain CLIB1...    70   6e-11
AF129873_1(AF129873|pid:none) Pichia angusta peroxin-1 (PEX1) ge...    66   1e-09
AP007171_529(AP007171|pid:none) Aspergillus oryzae RIB40 genomic...    61   5e-08
AM270369_32(AM270369|pid:none) Aspergillus niger contig An16c017...    60   1e-07
CU633438_729(CU633438|pid:none) Podospora anserina genomic DNA c...    56   1e-06
AJ243811_1(AJ243811|pid:none) Drosophila melanogaster mRNA for l...    54   4e-06
CU928167_107(CU928167|pid:none) Kluyveromyces thermotolerans str...    54   4e-06
CU928179_178(CU928179|pid:none) Zygosaccharomyces rouxii strain ...    50   6e-05
CR954202_592(CR954202|pid:none) Ostreococcus tauri strain OTTH05...    50   6e-05
(P24004) RecName: Full=Peroxisomal ATPase PEX1; AltName: Full=Pe...    45   0.003
CR382122_264(CR382122|pid:none) Kluyveromyces lactis strain NRRL...    42   0.022
CR380954_397(CR380954|pid:none) Candida glabrata strain CBS138 c...    41   0.038
CT005271_367(CT005271|pid:none) Leishmania major strain Friedlin...    40   0.084
T19208(T19208)hypothetical protein C11H1.4 - Caenorhabditis eleg...    40   0.11
AP009608_223(AP009608|pid:none) Mycoplasma fermentans PG18 DNA, ...    37   0.93
CP000111_1197(CP000111|pid:none) Prochlorococcus marinus str. MI...    35   3.5
CP000552_1705(CP000552|pid:none) Prochlorococcus marinus str. MI...    34   4.6
AC116957_99(AC116957|pid:none) Dictyostelium discoideum chromoso...    34   6.0
CP000698_4138(CP000698|pid:none) Geobacter uraniireducens Rf4, c...    34   6.0
CP000395_366(CP000395|pid:none) Borrelia afzelii PKo, complete g...    34   6.0
CP000263_248(CP000263|pid:none) Buchnera aphidicola str. Cc (Cin...    33   7.8

>(Q54GX5) RecName: Full=Peroxisome biogenesis factor 1; AltName:
           Full=Peroxin-1;
          Length = 1227

 Score =  462 bits (1190), Expect = e-129
 Identities = 231/254 (90%), Positives = 231/254 (90%)
 Frame = +2

Query: 14  MELHVQLKHSTDCFVSLPPKIVHXXXXXXXXXXXXXGTLGLEITWYDKINKKENKGYVGW 193
           MELHVQLKHSTDCFVSLPPKIVH             GTLGLEITWYDKINKKENKGYVGW
Sbjct: 1   MELHVQLKHSTDCFVSLPPKIVHSLLLLSEKQSKSLGTLGLEITWYDKINKKENKGYVGW 60

Query: 194 AGGSTDPRFTDSIEMSQEMAQCLGGIKNEQKLKLKALNNIELAHSVQVEPLTSDDWEIME 373
           AGGSTDPRFTDSIEMSQEMAQCLGGIKNEQKLKLKALNNIELAHSVQVEPLTSDDWEIME
Sbjct: 61  AGGSTDPRFTDSIEMSQEMAQCLGGIKNEQKLKLKALNNIELAHSVQVEPLTSDDWEIME 120

Query: 374 VHQQYLEEQLLNQVNILYSGQIVPIWIHHKTIIKLKVTETLPTPVVKLSSNSEIIVAPKP 553
           VHQQYLEEQLLNQVNILYSGQIVPIWIHHKTIIKLKVTETLPTPVVKLSSNSEIIVAPKP
Sbjct: 121 VHQQYLEEQLLNQVNILYSGQIVPIWIHHKTIIKLKVTETLPTPVVKLSSNSEIIVAPKP 180

Query: 554 RNLPXXXXXXXXXXISKETLKPRFLQIKDFKIDYNNNNTFINEIYINKELLNQFQWNIGD 733
           RNLP          ISKETLKPRFLQIKDFKIDYNNNNTFINEIYINKELLNQFQWNIGD
Sbjct: 181 RNLPTTTTSSQQQQISKETLKPRFLQIKDFKIDYNNNNTFINEIYINKELLNQFQWNIGD 240

Query: 734 IIEISKVSKNNNKN 775
           IIEISKVSKNNNKN
Sbjct: 241 IIEISKVSKNNNKN 254

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 837,546,975
Number of extensions: 13291140
Number of successful extensions: 39912
Number of sequences better than 10.0: 36
Number of HSP's gapped: 39831
Number of HSP's successfully gapped: 36
Length of query: 259
Length of database: 1,061,185,681
Length adjustment: 126
Effective length of query: 133
Effective length of database: 649,361,233
Effective search space: 86365043989
Effective search space used: 86365043989
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 31 (16.5 bits)
PSORT 

 psg: 0.74  gvh: 0.55  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: peroxisomal

>> prediction for Contig-U04220-1 is cyt
VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 1
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0