Contig-U04076-1
Contig ID Contig-U04076-1
Contig update 2001. 8.29
Contig sequence
>Contig-U04076-1 (Contig-U04076-1Q) /CSM_Contig/Contig-U04076-1Q.Seq.d
AATAATAATAATAACAACAATAGTAGTAATAATAATAATAATAACTCTTC
AAATATGTCAGAACCATCACCAATTTCAGCATTTTTTCATCAACCATCCA
CTCCATCAAAAACATTTAGAATGTTTGATAAAACACCATCAAGTCCATCA
ACACCATTAGGTTTCGATAAATTAACTCTTCCATCGATTTATTCCTCTTT
AGAAAATATTTCTCTTAACCCTTCAAATATTATGAATCATACTTCAACTT
CACTTTCAATCTCTAAACAACAAAAATTAAAAGAATCTGATTCTGATAAA
AAAGAAAAGAATGATGAATCTTCTTCAACCGGAGTTCCAATTGTTAAACA
AGAAGTCACTGAAGAAAAACAACAAGAATTAAAACAACAAGAATTAAAAC
AACAAGAATTAAAACAACAAGAATTAAAACAACAACAACAAGAGAAAGAA
GAAAAAGA

Gap no gap
Contig length 458
Chromosome number (1..6, M) 1
Chromosome length 4919822
Start point 3698083
End point 3697625
Strand (PLUS/MINUS) MINUS
Number of clones 1
Number of EST 1
Link to clone list U04076
List of clone(s)

est1=SLF241Z,1,459
Translated Amino Acid sequence
NNNNNNNSSNNNNNNSSNMSEPSPISAFFHQPSTPSKTFRMFDKTPSSPSTPLGFDKLTL
PSIYSSLENISLNPSNIMNHTSTSLSISKQQKLKESDSDKKEKNDESSSTGVPIVKQEVT
EEKQQELKQQELKQQELKQQELKQQQQEKEEK


Translated Amino Acid sequence (All Frames)
Frame A:
NNNNNNNSSNNNNNNSSNMSEPSPISAFFHQPSTPSKTFRMFDKTPSSPSTPLGFDKLTL
PSIYSSLENISLNPSNIMNHTSTSLSISKQQKLKESDSDKKEKNDESSSTGVPIVKQEVT
EEKQQELKQQELKQQELKQQELKQQQQEKEEK


Frame B:
iiiittivviiiiitlqicqnhhqfqhffinhplhqkhleclikhhqvhqhh*vsin*lf
hrfipl*kiflltlqil*iilqlhfqslnnkn*knlilikkkrmmnllqpefqllnkksl
kknnkn*nnkn*nnkn*nnkn*nnnnkrkkkk


Frame C:
****qq********lfkyvrtitnfsifsstihsikni*nv**ntiksintirfr*inss
idlflfrkyfs*pfkyyesyfnftfnl*ttkikri*f**krke**iffnrssnc*trsh*
rkttrikttrikttrikttrikttttrerrkr


own update 2004. 6. 9
Homology vs CSM-cDNA
Query= Contig-U04076-1 (Contig-U04076-1Q)
/CSM_Contig/Contig-U04076-1Q.Seq.d
(458 letters)

Database: CSM
6905 sequences; 5,674,871 total letters


Score E
Sequences producing significant alignments: (bits) Value

Contig-U04076-1 (Contig-U04076-1Q) /CSM_Contig/Conti... 622 e-179
Contig-U11307-1 (Contig-U11307-1Q) /CSM_Contig/Conti... 44 1e-04
Contig-U13239-1 (Contig-U13239-1Q) /CSM_Contig/Conti... 36 0.032
Contig-U12160-1 (Contig-U12160-1Q) /CSM_Contig/Conti... 36 0.032
Contig-U10804-1 (Contig-U10804-1Q) /CSM_Contig/Conti... 36 0.032
Contig-U01063-1 (Contig-U01063-1Q) /CSM_Contig/Conti... 36 0.032
Contig-U14340-1 (Contig-U14340-1Q) /CSM_Contig/Conti... 34 0.13
Contig-U13885-1 (Contig-U13885-1Q) /CSM_Contig/Conti... 34 0.13
Contig-U13101-1 (Contig-U13101-1Q) /CSM_Contig/Conti... 34 0.13
Contig-U12236-1 (Contig-U12236-1Q) /CSM_Contig/Conti... 34 0.13

>Contig-U04076-1 (Contig-U04076-1Q) /CSM_Contig/Contig-U04076-1Q.Seq.d
Length = 458

Score = 622 bits (314), Expect = e-179
Identities = 314/314 (100%)
Strand = Plus / Plus


Query: 45 ctcttcaaatatgtcagaaccatcaccaatttcagcattttttcatcaaccatccactcc 104
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 45 ctcttcaaatatgtcagaaccatcaccaatttcagcattttttcatcaaccatccactcc 104


Query: 105 atcaaaaacatttagaatgtttgataaaacaccatcaagtccatcaacaccattaggttt 164
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 105 atcaaaaacatttagaatgtttgataaaacaccatcaagtccatcaacaccattaggttt 164


Query: 165 cgataaattaactcttccatcgatttattcctctttagaaaatatttctcttaacccttc 224
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 165 cgataaattaactcttccatcgatttattcctctttagaaaatatttctcttaacccttc 224


Query: 225 aaatattatgaatcatacttcaacttcactttcaatctctaaacaacaaaaattaaaaga 284
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 225 aaatattatgaatcatacttcaacttcactttcaatctctaaacaacaaaaattaaaaga 284


Query: 285 atctgattctgataaaaaagaaaagaatgatgaatcttcttcaaccggagttccaattgt 344
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 285 atctgattctgataaaaaagaaaagaatgatgaatcttcttcaaccggagttccaattgt 344


Query: 345 taaacaagaagtca 358
||||||||||||||
Sbjct: 345 taaacaagaagtca 358


Score = 34.2 bits (17), Expect = 0.13
Identities = 17/17 (100%)
Strand = Plus / Plus


Query: 442 gagaaagaagaaaaaga 458
|||||||||||||||||
Sbjct: 442 gagaaagaagaaaaaga 458


>Contig-U11307-1 (Contig-U11307-1Q) /CSM_Contig/Contig-U11307-1Q.Seq.d
Length = 1908

Score = 44.1 bits (22), Expect = 1e-04
Identities = 22/22 (100%)
Strand = Plus / Plus


Query: 266 aacaacaaaaattaaaagaatc 287
||||||||||||||||||||||
Sbjct: 1501 aacaacaaaaattaaaagaatc 1522


Score = 34.2 bits (17), Expect = 0.13
Identities = 17/17 (100%)
Strand = Plus / Minus


Query: 311 atgatgaatcttcttca 327
|||||||||||||||||
Sbjct: 1385 atgatgaatcttcttca 1369


Score = 28.2 bits (14), Expect = 7.7
Identities = 14/14 (100%)
Strand = Plus / Plus


Query: 272 aaaaattaaaagaa 285
||||||||||||||
Sbjct: 1347 aaaaattaaaagaa 1360


>Contig-U13239-1 (Contig-U13239-1Q) /CSM_Contig/Contig-U13239-1Q.Seq.d
Length = 236

Score = 36.2 bits (18), Expect = 0.032
Identities = 18/18 (100%)
Strand = Plus / Plus


Query: 265 aaacaacaaaaattaaaa 282
||||||||||||||||||
Sbjct: 5 aaacaacaaaaattaaaa 22


Database: CSM
Posted date: Jun 9, 2004 7:35 PM
Number of letters in database: 5,674,871
Number of sequences in database: 6905

Lambda K H
1.37 0.711 1.31

Gapped
Lambda K H
1.37 0.711 1.31


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 8010
Number of Sequences: 6905
Number of extensions: 8010
Number of successful extensions: 948
Number of sequences better than 10.0: 348
length of query: 458
length of database: 5,674,871
effective HSP length: 16
effective length of query: 442
effective length of database: 5,564,391
effective search space: 2459460822
effective search space used: 2459460822
T: 0
A: 40
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 14 (28.2 bits)
dna update 2009. 6.13
Homology vs DNA
Query= Contig-U04076-1 (Contig-U04076-1Q) /CSM_Contig/Contig-U04076-1Q.Seq.d
(458 letters)

Database: ddbj_A
102,105,510 sequences; 101,790,757,118 total letters

Searching..................................................done

Score E
Sequences producing significant alignments: (bits) Value N

(AU052872) Dictyostelium discoideum slug cDNA, clone SLF241. 597 e-166 1
(CU928909) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f... 44 0.019 2
(CS543183) Sequence 32 from Patent WO2007014951. 48 0.29 1
(AR349007) Sequence 3618 from patent US 6583275. 48 0.29 1
(EK282373) 1095462300209 Global-Ocean-Sampling_GS-31-01-01-1... 48 0.29 1
(EK212715) 1095460104637 Global-Ocean-Sampling_GS-31-01-01-1... 48 0.29 1
(AC205780) Pongo abelii BAC clone CH276-455B23 from chromoso... 38 0.41 6
(AL929357) Plasmodium falciparum strain 3D7, chromosome 9; s... 40 0.43 8
(AC130772) Rattus norvegicus clone CH230-202M3, *** SEQUENCI... 36 0.62 2
(AC157002) Bos taurus clone CH240-69N13, WORKING DRAFT SEQUE... 36 0.62 2
(AC168014) Bos taurus clone CH240-159O9, WORKING DRAFT SEQUE... 36 0.62 2
(AC171333) Mus musculus chromosome 16 clone RP24-80G14, WORK... 34 0.62 2
(AC154631) Mus musculus BAC clone RP23-264F8 from 16, comple... 34 0.63 2
(AC140319) Mus musculus BAC clone RP23-413L6 from chromosome... 34 0.63 2
(BH158426) ENTSL91TF Entamoeba histolytica Sheared DNA Entam... 36 0.82 2
(CR788294) Zebrafish DNA sequence from clone DKEY-68A6 in li... 46 1.2 1
(CR383677) Zebrafish DNA sequence from clone CH211-157P16 in... 46 1.2 1
(BX004803) Zebrafish DNA sequence from clone CH211-119C20 in... 46 1.2 1
(AC110303) Rattus norvegicus clone CH230-223E3, *** SEQUENCI... 46 1.2 1
(FP236232) Zebrafish DNA sequence *** SEQUENCING IN PROGRESS... 46 1.2 1
(EK331347) 1095467024736 Global-Ocean-Sampling_GS-31-01-01-1... 46 1.2 1
(AJ563070) Cryptosporidium parvum GSS, PAC clone pica_0013_e... 46 1.2 1
(AJ563055) Cryptosporidium parvum GSS, PAC clone pica_0013_d... 46 1.2 1
(BM170661) EST573184 PyBS Plasmodium yoelii yoelii cDNA clon... 46 1.2 1
(BM169723) EST572246 PyBS Plasmodium yoelii yoelii cDNA clon... 46 1.2 1
(BM166807) EST569330 PyBS Plasmodium yoelii yoelii cDNA clon... 46 1.2 1
(BM166300) EST568823 PyBS Plasmodium yoelii yoelii cDNA clon... 46 1.2 1
(BM166131) EST568654 PyBS Plasmodium yoelii yoelii cDNA clon... 46 1.2 1
(BM165128) EST567651 PyBS Plasmodium yoelii yoelii cDNA clon... 46 1.2 1
(BM163791) EST566314 PyBS Plasmodium yoelii yoelii cDNA clon... 46 1.2 1
(BM162409) EST564932 PyBS Plasmodium yoelii yoelii cDNA clon... 46 1.2 1
(BM161197) EST563720 PyBS Plasmodium yoelii yoelii cDNA clon... 46 1.2 1
(BF298021) 059PbC09 Pb cDNA #17, Tommaso Pace, Marta Ponzi, ... 46 1.2 1
(BB980447) Plasmodium berghei str. ANKA cDNA clone: OK010619... 46 1.2 1
(BB979553) Plasmodium berghei str. ANKA cDNA clone: OK009648... 46 1.2 1
(GH609265) G1297P561FG10.T0 C. parvum KSU-1 normalized sporo... 46 1.2 1
(GH608011) G1297P558FL5.T0 C. parvum KSU-1 normalized sporoz... 46 1.2 1
(GH605247) G1297P554FB21.T0 C. parvum KSU-1 normalized sporo... 46 1.2 1
(GH604232) G1297P546FN24.T0 C. parvum KSU-1 normalized sporo... 46 1.2 1
(GH604103) G1297P546FG13.T0 C. parvum KSU-1 normalized sporo... 46 1.2 1
(GH601396) G1297P539FF17.T0 C. parvum KSU-1 normalized sporo... 46 1.2 1
(GH595033) G1297P523FC10.T0 C. parvum KSU-1 normalized sporo... 46 1.2 1
(GH592070) G1297P522FM11.T0 C. parvum KSU-1 normalized sporo... 46 1.2 1
(GH587985) G1297P516FO2.T0 C. parvum KSU-1 normalized sporoz... 46 1.2 1
(GH585310) G1297P54FA22.T0 C. parvum KSU-1 normalized sporoz... 46 1.2 1
(ET805110) CHO_OF700xb06f1.ab1 CHO_OF Nicotiana tabacum geno... 38 1.8 2
(AC184616) Strongylocentrotus purpuratus clone R3-3093F10, W... 34 2.3 4
(CU075420) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f... 34 2.3 2
(AC211069) Solanum lycopersicum chromosome 6 clone C06HBa028... 32 2.7 6
(AZ540483) ENTED68TF Entamoeba histolytica Sheared DNA Entam... 34 3.0 2
(AZ545300) ENTEV96TF Entamoeba histolytica Sheared DNA Entam... 34 3.0 2
(AC188199) Gossypium hirsutum chromosome UNKNOWN clone ZMMBB... 34 3.2 3
(AC189391) Brassica rapa subsp. pekinensis clone KBrB054G07,... 40 3.5 3
(AC020579) Arabidopsis thaliana chromosome 1 BAC F1O17 genom... 34 3.6 3
(AC180545) Strongylocentrotus purpuratus clone R3-3096C15, W... 34 3.7 5
(AC174334) Medicago truncatula clone mth2-154n22, WORKING DR... 40 4.4 2
(AC108784) Mus musculus chromosome 8, clone RP24-181O5, comp... 44 4.6 1
(AM462589) Vitis vinifera contig VV78X100780.3, whole genome... 44 4.6 1
(AC165519) Bos taurus clone CH240-171J18, WORKING DRAFT SEQU... 44 4.6 1
(FH380803) CHO_OF4740xo23f1.ab1 CHO_OF4 Nicotiana tabacum ge... 44 4.6 1
(CC560054) CH240_469C12.TARBAC13P2 CHORI-240 Bos taurus geno... 44 4.6 1
(DV537045) ZM_BFb0229M21.f ZM_BFb Zea mays cDNA 3', mRNA seq... 44 4.6 1
(DV196046) 5858 leuk Oncorhynchus mykiss cDNA clone 06RT_05M... 44 4.6 1
(AU270674) Dictyostelium discoideum vegetative cDNA clone:VS... 44 4.6 1
(DC199950) Plasmodium berghei cDNA clone:LV011393, liver sta... 44 4.6 1
(AU037199) Dictyostelium discoideum slug cDNA, clone SSB602. 44 4.6 1
(CK308989) SB02048A1H03.f1 normalized Keck-Tagu Library SB02... 44 4.6 1
(BJ345265) Dictyostelium discoideum cDNA clone:dda23l06, 3' ... 44 4.6 1
(FE147793) LV_HP_RA53N22f Litopenaeus vannamei hepatopancrea... 44 4.6 1
(AC181138) Strongylocentrotus purpuratus clone R3-16G10, WOR... 34 5.3 5
(CT311520) Sus scrofa genomic clone CH242-62J8, genomic surv... 30 5.9 3
(AL662822) Human DNA sequence from clone XXbac-17K10 on chro... 40 6.3 4
(CS741953) Sequence 9949 from Patent WO2005083127. 38 6.4 2
(CP000885) Clostridium phytofermentans ISDg, complete genome. 32 6.5 2
(AC026962) Homo sapiens chromosome 3 clone RP11-264B9 map 3,... 34 6.7 4
(AC023126) Homo sapiens chromosome 8 clone RP11-333H13, WORK... 36 6.7 7
(CU856245) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f... 34 7.0 5
(EK033520) 1092957894731 Global-Ocean-Sampling_GS-31-01-01-1... 40 7.3 2
(AC164771) Bos taurus clone CH240-144C13, *** SEQUENCING IN ... 30 8.0 2
(AC230285) Bos taurus clone CH240-513P5, WORKING DRAFT SEQUE... 32 8.3 2
(AC199303) Spermophilus tridecemlineatus clone VMRC20-685N13... 30 8.3 2
(DD334048) A method of testing a sensitivity of tumor cells ... 32 8.3 2
(AL162742) Human DNA sequence from clone RP11-127L20 on chro... 32 8.3 2
(AC172961) Bos taurus clone CH240-310H11, WORKING DRAFT SEQU... 32 8.3 2
(AC104656) Homo sapiens BAC clone RP11-547J14 from 4, comple... 40 8.5 3
(AC231246) Choloepus hoffmanni clone CH281-217H18, WORKING D... 36 8.5 4
(AC199688) Spermophilus tridecemlineatus clone VMRC20-171D23... 30 8.5 2
(CU468254) Zebrafish DNA sequence from clone CH73-374J3 in l... 32 8.6 2
(AB014078) Homo sapiens genomic DNA, chromosome 6p21.3, HLA ... 40 9.7 2

>(AU052872) Dictyostelium discoideum slug cDNA, clone SLF241.
Length = 459

Score = 597 bits (301), Expect = e-166
Identities = 301/301 (100%)
Strand = Plus / Plus


Query: 45 ctcttcaaatatgtcagaaccatcaccaatttcagcattttttcatcaaccatccactcc 104
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 45 ctcttcaaatatgtcagaaccatcaccaatttcagcattttttcatcaaccatccactcc 104


Query: 105 atcaaaaacatttagaatgtttgataaaacaccatcaagtccatcaacaccattaggttt 164
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 105 atcaaaaacatttagaatgtttgataaaacaccatcaagtccatcaacaccattaggttt 164


Query: 165 cgataaattaactcttccatcgatttattcctctttagaaaatatttctcttaacccttc 224
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 165 cgataaattaactcttccatcgatttattcctctttagaaaatatttctcttaacccttc 224


Query: 225 aaatattatgaatcatacttcaacttcactttcaatctctaaacaacaaaaattaaaaga 284
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 225 aaatattatgaatcatacttcaacttcactttcaatctctaaacaacaaaaattaaaaga 284


Query: 285 atctgattctgataaaaaagaaaagaatgatgaatcttcttcaaccggagttccaattgt 344
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 285 atctgattctgataaaaaagaaaagaatgatgaatcttcttcaaccggagttccaattgt 344


Query: 345 t 345
|
Sbjct: 345 t 345

Lambda K H
1.37 0.711 1.31

Matrix: blastn matrix:1 -3
Number of Sequences: 102105510
Number of Hits to DB: 499,301,465
Number of extensions: 39139893
Number of successful extensions: 3762673
Number of sequences better than 10.0: 91
Length of query: 458
Length of database: 101,790,757,118
Length adjustment: 23
Effective length of query: 435
Effective length of database: 99,442,330,388
Effective search space: 43257413718780
Effective search space used: 43257413718780
X1: 11 (21.8 bits)
S2: 21 (42.1 bits)

protein update 2009. 7.29
Homology vs Protein
Query= Contig-U04076-1 (Contig-U04076-1Q) /CSM_Contig/Contig-U04076-1Q.Seq.d
(458 letters)

Database: nrp_A
3,268,448 sequences; 1,061,185,681 total letters

Searching..................................................done

Score E
Sequences producing significant alignments: (bits) Value

BC166388_1(BC166388|pid:none) Xenopus tropicalis neurofilament 3... 34 1.8
AP003706_5(AP003706|pid:none) Oryza sativa Japonica Group genomi... 34 1.8
CU633873_40(CU633873|pid:none) Podospora anserina genomic DNA ch... 33 3.1
AM270295_28(AM270295|pid:none) Aspergillus niger contig An13c001... 33 3.1
AP004852_1(AP004852|pid:none) Oryza sativa Japonica Group genomi... 33 3.1
BC047248_1(BC047248|pid:none) Xenopus laevis middle molecular we... 33 4.1
U85969_1(U85969|pid:none) Xenopus laevis middle molecular weight... 33 4.1
BC074454_1(BC074454|pid:none) Xenopus laevis middle molecular we... 33 4.1
CP000361_725(CP000361|pid:none) Arcobacter butzleri RM4018, comp... 32 5.3
AC006618_1(AC006618|pid:none) Caenorhabditis elegans cosmid C45B... 32 9.1

>BC166388_1(BC166388|pid:none) Xenopus tropicalis neurofilament 3,
medium, mRNA (cDNA clone IMAGE:7603009), partial cds.
Length = 604

Score = 33.9 bits (76), Expect = 1.8
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 3/57 (5%)
Frame = +1

Query: 76 SAFFHQPSTPSKTFRMFDKTPSSPSTPLGFDK---LTLPSIYSSLENISLNPSNIMN 237
+++ STPS FR + SSPST + + L P +YSS +++ L+ S+ N
Sbjct: 21 TSYSRTSSTPSSGFRSQSWSRSSPSTISSYKRTPNLAAPRVYSSTDSLDLSQSSAFN 77

Lambda K H
0.310 0.125 0.353

Gapped
Lambda K H
0.267 0.0410 0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 414,077,818
Number of extensions: 6274863
Number of successful extensions: 14098
Number of sequences better than 10.0: 10
Number of HSP's gapped: 14062
Number of HSP's successfully gapped: 10
Length of query: 152
Length of database: 1,061,185,681
Length adjustment: 114
Effective length of query: 38
Effective length of database: 688,582,609
Effective search space: 26166139142
Effective search space used: 26166139142
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.4 bits)
S2: 29 (15.8 bits)

PSORT

psg: 0.61 gvh: 0.39 alm: 0.54 top: 0.53 tms: 0.00 mit: 0.31 mip: 0.08
nuc: 0.00 erl: 0.00 erm: 0.20 pox: 0.00 px2: 0.00 vac: 0.00 rnp: 0.00
act: 0.00 caa: 0.00 yqr: 0.00 tyr: 0.00 leu: 0.00 gpi: 0.00 myr: 0.00
dna: 0.00 rib: 0.00 bac: 0.00 m1a: 0.00 m1b: 0.00 m2 : 0.00 mNt: 0.00
m3a: 0.00 m3b: 0.00 m_ : 1.00

32.0 %: mitochondrial
32.0 %: nuclear
24.0 %: cytoplasmic
8.0 %: cytoskeletal
4.0 %: peroxisomal

>> prediction for Contig-U04076-1 is mit

VS (DIR, S) 0
VH (FL, L) 0
VF (FL, S) 0
AH (FL, L) 0
AF (FL, S) 0
SL (DIR, L) 1
SS (DIR, S) 0
SH (FL, L) 0
SF (FL, S) 0
CH (FL, L) 0
CF (FL, S) 0
FCL (DIR, L) 0
FC (DIR, S) 0
FC-IC (SUB) 0