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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFC104
Library AF
(Link to library)
Clone ID AFC104
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16509-1
Original site URL
Representative seq. ID AFC104P
(Link to Original site)
Representative DNA sequence 
>AFC104 (AFC104Q) /CSM/AF/AFC1-A/AFC104Q.Seq.d/
AAAAATGTCAAATAAATAAAAAAAAAAAAAAAAAAATTNAAAAAAATTTTANTNGGTTTN
AAAAGGGNTTNGTTTTAATAAAAAAAAAAAAAANCCCCNTTTTTTTNGGGGNAAAAAAAA
ANTAAAAATTAAAAAAAAAAXXXXXXXXXXTNNTTTTNAAAAAAAAAAAAAAAANTNAAA
AAATTTTTTTTAGTTTTTAAAANGGNAANTTTTTNAAAAAAAAAAAAAAAAACCCCTTTT
NTTTNTNGNTAAAAAAAAATTAAAAATTAAAAAAAAAAAAAATNAAAAAAAATNATNATT
AGCTATTCCATGGGNCTTANAGAAAATNAAAAAGTTTNAAAAAAAAATNAAAAATAATNA
AAAATAAAAAAAAAATAAAAAAAAAAACTTTTAAAACTCTCCTTCTACTTTTTGGTGCTT
CTGTTAAGCTAATGCTAAGGTGTCTCTCTCCCCCTTTGCTTTGCATTACTCTCCTCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
kmsnk*KKKKKNXKKFXXVXKGXXFNKKKKXPXFFXGKKKXKIKKK---

---XFXKKKKKXKKFFLVFKXXXFXKKKKKTPFXXX*kkikn*kkkkxkkxxisysmglx
enxkvxkknxk*xkikkk*kkkllklsfyflvllls*c*gvslplcfallss
Translated Amino Acid sequence (All Frames) 
Frame A:
knvk*ikkkkkkxkkilxgxkrxxf**kkkkxpffxgxkkxkn*kk---

---XFXKKKKKXKKFFLVFKXXXFXKKKKKTPFXXX*kkikn*kkkkxkkxxisysmglx
enxkvxkknxk*xkikkk*kkkllklsfyflvllls*c*gvslplcfallss

Frame B:
kmsnk*KKKKKNXKKFXXVXKGXXFNKKKKXPXFFXGKKKXKIKKK---

---xfxkkkkxxknff*flkxxxfxkkkkkplxfxxkkklkikkkknxkkxxlaipwxlx
kxkkfxkkxknnxk*kknkkknf*nspstfwcfc*anakvslspfalhysp

Frame C:
kcqinkkkkkkixknfxxfxkgxvlikkkkxpxfxgxkkx*klkkk---

---xxkkkkkxxkiffsf*xgxfxkkkkknpfxxxxkkn*klkkkkxkknxx*lfhgxxr
kxksxkkkxkixknkkkikkktfktllllfgasvklmlrclsppllcitll
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSC737 (VSC737Q) /CSM/VS/VSC7-B/VSC737Q.Seq.d/                        178   5e-44
VSA585 (VSA585Q) /CSM/VS/VSA5-D/VSA585Q.Seq.d/                        178   5e-44
SSM803 (SSM803Q) /CSM/SS/SSM8-A/SSM803Q.Seq.d/                        178   5e-44
SSM728 (SSM728Q) /CSM/SS/SSM7-B/SSM728Q.Seq.d/                        178   5e-44
SSM719 (SSM719Q) /CSM/SS/SSM7-A/SSM719Q.Seq.d/                        178   5e-44
SSM276 (SSM276Q) /CSM/SS/SSM2-D/SSM276Q.Seq.d/                        178   5e-44
SSM269 (SSM269Q) /CSM/SS/SSM2-C/SSM269Q.Seq.d/                        178   5e-44
SSM229 (SSM229Q) /CSM/SS/SSM2-B/SSM229Q.Seq.d/                        178   5e-44
SSM165 (SSM165Q) /CSM/SS/SSM1-C/SSM165Q.Seq.d/                        178   5e-44
SSL845 (SSL845Q) /CSM/SS/SSL8-B/SSL845Q.Seq.d/                        178   5e-44
own update 2004. 8.10
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D16417|D16417.1 Dictyostelium discoideum mRNA.                   178  7e-44  2
BZ463651|BZ463651.1 BONRK12TR BO_1.6_2_KB_tot Brassica oleracea genomic clone BONRK12, DNA sequence.    50  0.025  1
AC113975|AC113975.4 Mus musculus clone RP23-140P24, WORKING DRAFT SEQUENCE, 5 ordered pieces.    42  6.0  1
AC093987|AC093987.6 Rattus norvegicus clone CH230-36D18, WORKING DRAFT SEQUENCE, 5 unordered pieces.    42  6.0  1
BX092198|BX092198.1 RZPD Homo sapiens cDNA clone IMAGp998E11743 = IMAGE:327538 5' EST.    38  8.7  2
dna update 2003. 7.24
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 242,962,956
protein update 2009. 3.27
PSORT 

 psg: 0.74  gvh: 0.24  alm: 0.66  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 1.20  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 84.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: cytoplasmic

>> prediction for AFC104 is nuc
5' end seq. ID AFC104F
5' end seq. 
>AFC104F.Seq
AAAAATGTCAAATAAATAAAAAAAAAAAAAAAAAAATTNAAAAAAATTTTANTNGGTTTN
AAAAGGGNTTNGTTTTAATAAAAAAAAAAAAAANCCCCNTTTTTTTNGGGGNAAAAAAAA
ANTAAAAATTAAAAAAAAAA----------
Length of 5' end seq. 140
3' end seq. ID AFC104Z
3' end seq. 
>AFC104Z.Seq
----------TNNTTTTNAAAAAAAAAAAAAAAANTNAAAAAATTTTTTTTAGTTTTTAA
AANGGNAANTTTTTNAAAAAAAAAAAAAAAAACCCCTTTTNTTTNTNGNTAAAAAAAAAT
TAAAAATTAAAAAAAAAAAAAATNAAAAAAAATNATNATTAGCTATTCCATGGGNCTTAN
AGAAAATNAAAAAGTTTNAAAAAAAAATNAAAAATAATNAAAAATAAAAAAAAAATAAAA
AAAAAAACTTTTAAAACTCTCCTTCTACTTTTTGGTGCTTCTGTTAAGCTAATGCTAAGG
TGTCTCTCTCCCCCTTTGCTTTGCATTACTCTCCTCA
Length of 3' end seq. 327
Connected seq. ID AFC104P
Connected seq. 
>AFC104P.Seq
AAAAATGTCAAATAAATAAAAAAAAAAAAAAAAAAATTNAAAAAAATTTTANTNGGTTTN
AAAAGGGNTTNGTTTTAATAAAAAAAAAAAAAANCCCCNTTTTTTTNGGGGNAAAAAAAA
ANTAAAAATTAAAAAAAAAA----------TNNTTTTNAAAAAAAAAAAAAAAANTNAAA
AAATTTTTTTTAGTTTTTAAAANGGNAANTTTTTNAAAAAAAAAAAAAAAAACCCCTTTT
NTTTNTNGNTAAAAAAAAATTAAAAATTAAAAAAAAAAAAAATNAAAAAAAATNATNATT
AGCTATTCCATGGGNCTTANAGAAAATNAAAAAGTTTNAAAAAAAAATNAAAAATAATNA
AAAATAAAAAAAAAATAAAAAAAAAAACTTTTAAAACTCTCCTTCTACTTTTTGGTGCTT
CTGTTAAGCTAATGCTAAGGTGTCTCTCTCCCCCTTTGCTTTGCATTACTCTCCTCA
Length of connected seq. 467
Full length Seq ID -
Full length Seq. -
Length of full length seq. -