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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AHB208
Library AH
(Link to library)
Clone ID AHB208
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16429-1
Original site URL
Representative seq. ID AHB208P
(Link to Original site)
Representative DNA sequence 
>AHB208 (AHB208Q) /CSM/AH/AHB2-A/AHB208Q.Seq.d/
ATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGA
TGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCC
AAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTG
TGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTXXXXXXXXXXGATAGTATT
ACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTAT
TTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAAT
GATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCA
GCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCT
GCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGC
TCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTC
ATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCA
TTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTC
AATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTACC
ACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAA
TATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTT
TTTTTAATATAAAATAAAAAAAAATAATATTATTTAAAACAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
YKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESAVRNC
DNFEVHEKDVENI---

---DSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRI
VNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYL
QGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTG
QTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylkq
k
Translated Amino Acid sequence (All Frames) 
Frame A:
i*itlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgisss*l
**l*strkrc*ky---

---DSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRI
VNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYL
QGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTG
QTVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylkq
k

Frame B:
YKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLESAVRNC
DNFEVHEKDVENI---

---ivlpliifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpilvl
linwlqplvhkqpmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkvhtf
kelsvslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpslklv
kp*rlplivvnllklsfvsilqsklnigqimvsylmf*enfyinfff*ykikknnii*nk

Frame C:
inylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwnqqfviv
itlkytkkmlkis---

---*yyh*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqysyc
**igssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrsips
rn*vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**iph*nws
nrkgyh*lw*if*nypsfrysnrs*ilgk*wylilcfkktftlisffnik*kkiilfktk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ461 (VHQ461Q) /CSM/VH/VHQ4-C/VHQ461Q.Seq.d/                       1356   0.0
VHQ432 (VHQ432Q) /CSM/VH/VHQ4-B/VHQ432Q.Seq.d/                       1356   0.0
VHQ267 (VHQ267Q) /CSM/VH/VHQ2-C/VHQ267Q.Seq.d/                       1356   0.0
VHO844 (VHO844Q) /CSM/VH/VHO8-B/VHO844Q.Seq.d/                       1356   0.0
VHO678 (VHO678Q) /CSM/VH/VHO6-D/VHO678Q.Seq.d/                       1356   0.0
VHK111 (VHK111Q) /CSM/VH/VHK1-A/VHK111Q.Seq.d/                       1356   0.0
VHD137 (VHD137Q) /CSM/VH/VHD1-B/VHD137Q.Seq.d/                       1356   0.0
VHA714 (VHA714Q) /CSM/VH/VHA7-A/VHA714Q.Seq.d/                       1356   0.0
VHA224 (VHA224Q) /CSM/VH/VHA2-A/VHA224Q.Seq.d/                       1356   0.0
SHL826 (SHL826Q) /CSM/SH/SHL8-B/SHL826Q.Seq.d/                       1356   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    56   6e-21  4
BQ511380|BQ511380.2 EST618795 Generation of a set of potato cDNA clones for microarray analyses mixed potato tissues Solanum tuberosum cDNA clone STMHR19 5' end, mRNA sequence.    56   2e-13  3
BI927352|BI927352.1 EST547241 tomato flower, 3 - 8 mm buds Lycopersicon esculentum cDNA clone cTOB22E7 5' end, mRNA sequence.    56   3e-12  3
AW622039|AW622039.1 EST312837 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX14G21 5', mRNA sequence.    56   4e-12  3
CK719688|CK719688.1 19969 Swollen Stolon Solanum tuberosum cDNA, mRNA sequence.    56   4e-12  3
BG889764|BG889764.1 EST515615 cSTD Solanum tuberosum cDNA clone cSTD15K20 5' sequence, mRNA sequence.    56   5e-12  3
BU032661|BU032661.1 QHJ21N12.yg.ab1 QH_EFGHJ sunflower RHA280 Helianthus annuus cDNA clone QHJ21N12, mRNA sequence.    54   2e-11  3
AY250116|AY250116.1 Lycopersicon pennellii aconitase mRNA, partial cds.    58   6e-11  3
AI775579|AI775579.1 EST256679 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER15P4, mRNA sequence.    56   7e-11  2
AW455290|AW455290.1 EST311828 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX10C9 5', mRNA sequence.    52   1e-09  2
dna update 2004. 2.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   443   e-123
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   292   1e-77
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   290   4e-77
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   288   1e-76
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   288   2e-76
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   288   2e-76
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   285   1e-75
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   285   2e-75
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   283   4e-75
AY050485_1(AY050485|pid:none) Prunus avium putative aconitase mR...   282   1e-74
protein update 2009. 4.27
PSORT 

 psg: 0.66  gvh: 0.31  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for AHB208 is cyt
5' end seq. ID AHB208F
5' end seq. 
>AHB208F.Seq
ATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGA
TGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCC
AAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTG
TGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTNNNNNNNNNN
Length of 5' end seq. 231
3' end seq. ID AHB208Z
3' end seq. 
>AHB208Z.Seq
NNNNNNNNNNGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAA
ATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTA
TGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTAT
TGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTT
AATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTT
GGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCT
TCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGT
TGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCT
CACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGG
TCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGA
TACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAA
ACTTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAATATTATTTAAAACA
AAAA
Length of 3' end seq. 724
Connected seq. ID AHB208P
Connected seq. 
>AHB208P.Seq
ATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCATTTGATAAAGTAAAGGA
TGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCC
AAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTG
TGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCT----------GATAGTATT
ACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAATCATCAGCCGCTCGTTAT
TTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATGGATCTCGTAGAGGTAAT
GATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCA
GCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCT
GCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGC
TCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTTCAAGGAATTAAGTGTGTC
ATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCA
TTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTCACTGGTAAAGAACAATTC
AATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGTCAAACCGTAAAGGTTACC
ACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGATACTCCAATCGAAGTTGAA
TATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTT
TTTTTAATATAAAATAAAAAAAAATAATATTATTTAAAACAAAAA
Length of connected seq. 935
Full length Seq ID -
Full length Seq. -
Length of full length seq. -