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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSK450
Library VS
(Link to library)
Clone ID VSK450
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U01944-1
Original site URL
Representative seq. ID VSK450P
(Link to Original site)
Representative DNA sequence 
>VSK450 (VSK450Q) /CSM/VS/VSK4-C/VSK450Q.Seq.d/
AAAATGGAATCTAAAAGTGAAATTAATTTTATCTTAAATAAAACCAAAGTTCCACCAGTC
ATTTTCGGAGGAATCTTTTCAACATTTGGAGTTGATAAAAATATGGATTATTATTATAAC
TATAGTGATTACCAAAATAAAAATAATAATAATAATGATAGAAACTATACAAAAAATAAT
AAAATAATCAATAGTTGTCAAACTGTTCAATGTAATAATTTATCATACTTATTACACTAA
AATTAAAAAAAAAAXXXXXXXXXXAAAATGGAATCTAAAAGTGAAATTAATTTTATCTTA
AATAAAACCAAAGTTCCACCAGTCATTTTCGGAGGAATCTTTTCAACATTTGGAGTTGAT
AAAAATATGGATTATTATTATAACTATAGTGATTACCAAAATAAAAATAATAATAATAAT
GATAGAAACTATACAAAAAATAATAAAATAATCAATAGTTGTCAAACTGTTCAATGTAAT
AATTTATCATACTTA
sequence update 2001. 3.22
Translated Amino Acid sequence 
kwnlkvklils*ikpkfhqsfseesfqhlelikiwiiiitivitkikiiiimietiqkii
k*SIVVKLFNVIIYHTYYTKIKKK---

---KMESKSEINFILNKTKVPPVIFGGIFSTFGVDKNMDYYYNYSDYQNKNNNNNDRNYT
KNNKIINSCQTVQCNNLSYL
Translated Amino Acid sequence (All Frames) 
Frame A:
kmeskseinfilnktkvppvifggifstfgvdknmdyyynysdyqnknnnnndrnytknn
kiinscqtvqcnnlsyllh*n*kk---

---KMESKSEINFILNKTKVPPVIFGGIFSTFGVDKNMDYYYNYSDYQNKNNNNNDRNYT
KNNKIINSCQTVQCNNLSYL

Frame B:
kwnlkvklils*ikpkfhqsfseesfqhlelikiwiiiitivitkikiiiimietiqkii
k*SIVVKLFNVIIYHTYYTKIKKK---

---kwnlkvklils*ikpkfhqsfseesfqhlelikiwiiiitivitkikiiiimietiq
kiik*sivvklfnviiyht

Frame C:
ngi*k*n*fylk*nqsstshfrrnlfniws**kyglll*l**lpk*k******klykk**
nnq*lsncsm**fiilitlklkkk---

---ngi*k*n*fylk*nqsstshfrrnlfniws**kyglll*l**lpk*k******klyk
k**nnq*lsncsm**fiil
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK450 (VSK450Q) /CSM/VS/VSK4-C/VSK450Q.Seq.d/                        264   1e-69
SSL422 (SSL422Q) /CSM/SS/SSL4-A/SSL422Q.Seq.d/                         80   4e-14
VHJ776 (VHJ776Q) /CSM/VH/VHJ7-D/VHJ776Q.Seq.d/                         36   0.48
VHH460 (VHH460Q) /CSM/VH/VHH4-C/VHH460Q.Seq.d/                         36   0.48
SSI722 (SSI722Q) /CSM/SS/SSI7-A/SSI722Q.Seq.d/                         36   0.48
SLK595 (SLK595Q) /CSM/SL/SLK5-D/SLK595Q.Seq.d/                         36   0.48
SHA826 (SHA826Q) /CSM/SH/SHA8-B/SHA826Q.Seq.d/                         36   0.48
CHL137 (CHL137Q) /CSM/CH/CHL1-B/CHL137Q.Seq.d/                         36   0.48
AHD567 (AHD567Q) /CSM/AH/AHD5-C/AHD567Q.Seq.d/                         36   0.48
VSF851 (VSF851Q) /CSM/VS/VSF8-C/VSF851Q.Seq.d/                         34   1.9
own update 2004. 8. 8
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

N52908|HUMALNE461 Human carcinoma cell-derived Alu RNA transcript, (rRNA); gb:J04970 CARBOXYPEPTIDASE M PRECURSOR (HUMAN);, mRNA sequence.    38  0.019  2
AI499298|AI499298.1 to09g09.x1 NCI_CGAP_Ut2 Homo sapiens cDNA clone IMAGE:2178592 3' similar to contains Alu repetitive element;contains element MER6 repetitive element ;, mRNA sequence.    38  0.024  2
AK094942|AK094942.1 Homo sapiens cDNA FLJ37623 fis, clone BRCOC2014013.    38  0.055  2
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    36  0.22  10
BM416365|BM416365.1 OP21456 Mixed Stage EST's from Globodera pallida, the potato cyst nematode Globodera pallida cDNA similar to Homology:(blast score <=3D 1e-5) Caenorhabditis elegans cosmid F56H6, mRNA sequence.    38  0.37  2
AE014850|AE014850.1 Plasmodium falciparum 3D7 chromosome 12, section 7 of 9 of the complete sequence.    32  0.43  9
BM416271|BM416271.1 OP21360 Mixed Stage EST's from Globodera pallida, the potato cyst nematode Globodera pallida cDNA similar to Homology:(blast score <=3D 1e-5) Caenorhabditis elegans cosmid F56H6, mRNA sequence.    38  0.52  2
Z98551|Z98551.1 Plasmodium falciparum MAL3P6.                     30  0.53  8
BX108299|BX108299.1 RZPD Homo sapiens cDNA clone IMAGp998G234212 = IMAGE:1659694 5' EST.    34  0.68  2
BX511212|BX511212.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-101B18.    34  0.79  8
dna update 2003. 7.30
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 429,105,359
protein update 2009. 3.26
PSORT 

 psg: 0.82  gvh: 0.38  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VSK450 is cyt
5' end seq. ID VSK450F
5' end seq. 
>VSK450F.Seq
AAAATGGAATCTAAAAGTGAAATTAATTTTATCTTAAATAAAACCAAAGTTCCACCAGTC
ATTTTCGGAGGAATCTTTTCAACATTTGGAGTTGATAAAAATATGGATTATTATTATAAC
TATAGTGATTACCAAAATAAAAATAATAATAATAATGATAGAAACTATACAAAAAATAAT
AAAATAATCAATAGTTGTCAAACTGTTCAATGTAATAATTTATCATACTTATTACACTAA
AATTAAAAAAAAAA----------
Length of 5' end seq. 254
3' end seq. ID VSK450Z
3' end seq. 
>VSK450Z.Seq
----------AAAATGGAATCTAAAAGTGAAATTAATTTTATCTTAAATAAAACCAAAGT
TCCACCAGTCATTTTCGGAGGAATCTTTTCAACATTTGGAGTTGATAAAAATATGGATTA
TTATTATAACTATAGTGATTACCAAAATAAAAATAATAATAATAATGATAGAAACTATAC
AAAAAATAATAAAATAATCAATAGTTGTCAAACTGTTCAATGTAATAATTTATCATACTT
A
Length of 3' end seq. 231
Connected seq. ID VSK450P
Connected seq. 
>VSK450P.Seq
AAAATGGAATCTAAAAGTGAAATTAATTTTATCTTAAATAAAACCAAAGTTCCACCAGTC
ATTTTCGGAGGAATCTTTTCAACATTTGGAGTTGATAAAAATATGGATTATTATTATAAC
TATAGTGATTACCAAAATAAAAATAATAATAATAATGATAGAAACTATACAAAAAATAAT
AAAATAATCAATAGTTGTCAAACTGTTCAATGTAATAATTTATCATACTTATTACACTAA
AATTAAAAAAAAAA----------AAAATGGAATCTAAAAGTGAAATTAATTTTATCTTA
AATAAAACCAAAGTTCCACCAGTCATTTTCGGAGGAATCTTTTCAACATTTGGAGTTGAT
AAAAATATGGATTATTATTATAACTATAGTGATTACCAAAATAAAAATAATAATAATAAT
GATAGAAACTATACAAAAAATAATAAAATAATCAATAGTTGTCAAACTGTTCAATGTAAT
AATTTATCATACTTA
Length of connected seq. 485
Full length Seq ID -
Full length Seq. -
Length of full length seq. -