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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSK324
Library VS
(Link to library)
Clone ID VSK324
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U15575-1
Original site URL
Representative seq. ID VSK324F
(Link to Original site)
Representative DNA sequence 
>VSK324 (VSK324Q) /CSM/VS/VSK3-A/VSK324Q.Seq.d/
GTAAAATTAAAACAAGAAGGTTTTCAACAACAACAACAACAACAACAACAATCATCACAA
CAAAGATTAAATAATAGTAATAATAATAATAATAATAATAATAATAATAATAATAATAAT
AATAATAATAATAATAATAATAATAAGTCGTCTTCCAAACTTTAATTACTTTTCANATGG
AAAATCTGANNCTGATGAAGAAGATTTAGAANATGANCACAATANTCGNTTAAAGAATTT
TTCTTTNTCTAAACGTCAATTNGAAAGANATGAAGAACAAGAAGAGCAACAACAACAACA
NGATGATGATAATAAAAATAATAATAACGAGACCTTTAAANAACAACAACAACACCAACA
AAAAXXXXXXXXXX
sequence update 2001. 3.22
Translated Amino Acid sequence 
*n*nkkvfnnnnnnnnnhhnkd*IIVIIIIIIIIIIIIIIIIIIIIIISRLPNFNYFSXG
KSXXDEEDLEXXHNXRLKNFSXSKRQXERXEEQEEQQQQXDDDNKNNNNETFKXQQQHQQ
---
Translated Amino Acid sequence (All Frames) 
Frame A:
vklkqegfqqqqqqqqqssqqrlnnsnnnnnnnnnnnnnnnnnnnnnnkssskl*llfxw
ki*x**rrfrx*xqxsxkefffx*tsixkx*rtrrattttx****k***rdl*xttttpt
k---


Frame B:
*n*nkkvfnnnnnnnnnhhnkd*IIVIIIIIIIIIIIIIIIIIIIIIISRLPNFNYFSXG
KSXXDEEDLEXXHNXRLKNFSXSKRQXERXEEQEEQQQQXDDDNKNNNNETFKXQQQHQQ
---


Frame C:
kiktrrfsttttttttiittkik*************************vvfqtlitfxme
nlxlmkki*xmxtixx*riflxlnvnxkxmknkksnnnnxmmiikiiitrplxnnnntnk
---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK324 (VSK324Q) /CSM/VS/VSK3-A/VSK324Q.Seq.d/                        361   5e-99
VSB896 (VSB896Q) /CSM/VS/VSB8-D/VSB896Q.Seq.d/                         46   4e-04
AHD721 (AHD721Q) /CSM/AH/AHD7-A/AHD721Q.Seq.d/                         46   4e-04
VSH244 (VSH244Q) /CSM/VS/VSH2-B/VSH244Q.Seq.d/                         44   0.001
VSF660 (VSF660Q) /CSM/VS/VSF6-C/VSF660Q.Seq.d/                         44   0.001
VHH470 (VHH470Q) /CSM/VH/VHH4-C/VHH470Q.Seq.d/                         44   0.001
VHG434 (VHG434Q) /CSM/VH/VHG4-B/VHG434Q.Seq.d/                         44   0.001
VHB308 (VHB308Q) /CSM/VH/VHB3-A/VHB308Q.Seq.d/                         44   0.001
VFM532 (VFM532Q) /CSM/VF/VFM5-B/VFM532Q.Seq.d/                         44   0.001
VFM148 (VFM148Q) /CSM/VF/VFM1-B/VFM148Q.Seq.d/                         44   0.001
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.   351  7e-98  3
BX231032|BX231032.1 Danio rerio genomic clone DKEY-254O18, genomic survey sequence.    44  2e-09  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    42  2e-07  4
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    44  1e-06  3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    42  2e-06  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.    40  7e-06  4
BM425114|BM425114.1 IpSpn01137 Spleen cDNA library Ictalurus punctatus cDNA 5', mRNA sequence.    44  9e-06  2
AC116921|AC116921.2 Dictyostelium discoideum chromosome 2 map 4624505-4657775 strain AX4, complete sequence.    44  2e-05  4
Z37981|Z37981.1 D.discoideum gene for protein kinase.             40  2e-05  3
AC115685|AC115685.1 Dictyostelium discoideum chromosome 2 map 4718821-4752388 strain AX4, complete sequence.    38  3e-05  5
dna update 2003. 7.28
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 112,894,237
protein update 2009. 3.26
PSORT 

 psg: 1.01  gvh: 0.38  alm: -0.10  top: 0.60  tms: 0.07  mit: 0.29  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VSK324 is nuc
5' end seq. ID VSK324F
5' end seq. 
>VSK324F.Seq
GTAAAATTAAAACAAGAAGGTTTTCAACAACAACAACAACAACAACAACAATCATCACAA
CAAAGATTAAATAATAGTAATAATAATAATAATAATAATAATAATAATAATAATAATAAT
AATAATAATAATAATAATAATAATAAGTCGTCTTCCAAACTTTAATTACTTTTCANATGG
AAAATCTGANNCTGATGAAGAAGATTTAGAANATGANCACAATANTCGNTTAAAGAATTT
TTCTTTNTCTAAACGTCAATTNGAAAGANATGAAGAACAAGAAGAGCAACAACAACAACA
NGATGATGATAATAAAAATAATAATAACGAGACCTTTAAANAACAACAACAACACCAACA
AAAA----------
Length of 5' end seq. 364
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -