>VSK255 (VSK255Q) /CSM/VS/VSK2-C/VSK255Q.Seq.d/
CTGATAAATNAAAATTATCAAAACGTCAAGGTGATGTTTCAGTTGATTCCTACATCTCAA
AAGGTTATTTACCAGAAGCATTAATTAATTTCGTTTCATTTTTAGGTTGGTCACCATCAA
CATCAACAACATCATCAACAACAACAACAACAACAACAACAACTGATCAAGAAACAAGTT
CAAAAGAAATATTTACACTTAAAGAATTGGTTGAATCATTTTCATTAGATGGTATTAATA
AGAGTGGTAGTGTTGTTAATATGGAGCGTTTAGATTGGTTAAATGTTAATCATATTAGAT
TACAAATTGATGATCCATTAAAACAAAATGAAATTTTATTAAAAATTAAAAATCAATTAT
TAGAATATTTTAATAATAATAATAATATAGATTTTAAAAAAGAAATTGATACAGAATATT
TATTAAAATCAATTAACTGTGTTAAAGAAAGAGTTCATTTAATTGAAGATTTTAATAACT
TATTAAAACCATTTTTTATTGATCAAATCGATTATACAACTGAAGAAGCAATTAAAATGA
AACAAAAAGTTTGGAAXXXXXXXXXX

DKXKLSKRQGDVSVDSYISKGYLPEALINFVSFLGWSPSTSTTSSTTTTTTTTTDQETSS
KEIFTLKELVESFSLDGINKSGSVVNMERLDWLNVNHIRLQIDDPLKQNEILLKIKNQLL
EYFNNNNNIDFKKEIDTEYLLKSINCVKERVHLIEDFNNLLKPFFIDQIDYTTEEAIKMK
QKVW---

Frame A:
linxnyqnvkvmfqliptsqkviyqkh*lisfhf*vghhqhqqhhqqqqqqqqqlikkqv
qkkylhlknwlnhfh*mvlirvvvlliwsv*ig*mliildyklmih*nkmkfy*klkiny
*niliiiii*ilkkkliqniy*nqltvlkkefi*lkility*nhflliksiiqlkkqlk*
nkkfg---


Frame B:
**xkiiktsr*cfs*flhlkrlftrsin*frfifrlvtininniinnnnnnnnn*srnkf
krniyt*rig*iifirwy**ew*cc*ygafrlvkc*sy*itn**siktk*nfikn*ksii
rif*****yrf*krn*yrifikin*lc*rkssfn*rf**liktify*snrlyn*rsn*ne
tksl---


Frame C:
DKXKLSKRQGDVSVDSYISKGYLPEALINFVSFLGWSPSTSTTSSTTTTTTTTTDQETSS
KEIFTLKELVESFSLDGINKSGSVVNMERLDWLNVNHIRLQIDDPLKQNEILLKIKNQLL
EYFNNNNNIDFKKEIDTEYLLKSINCVKERVHLIEDFNNLLKPFFIDQIDYTTEEAIKMK
QKVW---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK255 (VSK255Q) /CSM/VS/VSK2-C/VSK255Q.Seq.d/                        335   4e-91
VSG370 (VSG370Q) /CSM/VS/VSG3-C/VSG370Q.Seq.d/                         40   0.036
CHJ648 (CHJ648Q) /CSM/CH/CHJ6-B/CHJ648Q.Seq.d/                         40   0.036
VSJ174 (VSJ174Q) /CSM/VS/VSJ1-D/VSJ174Q.Seq.d/                         38   0.14
VSI640 (VSI640Q) /CSM/VS/VSI6-B/VSI640Q.Seq.d/                         38   0.14
VHM257 (VHM257Q) /CSM/VH/VHM2-C/VHM257Q.Seq.d/                         38   0.14
VHK159 (VHK159Q) /CSM/VH/VHK1-C/VHK159Q.Seq.d/                         38   0.14
VHG472 (VHG472Q) /CSM/VH/VHG4-C/VHG472Q.Seq.d/                         38   0.14
VHE665 (VHE665Q) /CSM/VH/VHE6-C/VHE665Q.Seq.d/                         38   0.14
FCL-AC15 (FCL-AC15Q) /CSM/FCL/FCL-AC/FCL-AC15Q.Seq.d/                  38   0.14

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116925|AC116925.2 Dictyostelium discoideum chromosome 2 map 4189423-4228998 strain AX4, complete sequence.   335  0.0  3
AE014824|AE014824.1 Plasmodium falciparum 3D7 chromosome 14 section 9 of 13 of the complete sequence.    38  0.049  7
AE014827|AE014827.1 Plasmodium falciparum 3D7 chromosome 14 section 12 of 13 of the complete sequence.    36  0.11  6
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    34  0.24  7
AL954657|AL954657.8 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-223E21.    42  0.25  5
CA899772|CA899772.1 PCEP01646 Scarlet Runner Bean Embryo-Proper Region Phaseolus coccineus cDNA 5' similar to No significant hits, mRNA sequence.    42  0.27  2
AX413861|AX413861.1 Sequence 852 from Patent WO0228891.           44  0.27  2
AX415776|AX415776.1 Sequence 2767 from Patent WO0228891.          44  0.28  2
AL928880|AL928880.5 Zebrafish DNA sequence from clone CH211-69P21 in linkage group 7.    40  0.28  3
U41540|U41540.1 Caenorhabditis elegans cosmid F35H12, complete sequence.    38  0.36  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116925_17(AC116925|pid:none) Dictyostelium discoideum chromoso...   317   2e-85
CP001108_1730(CP001108|pid:none) Prosthecochloris aestuarii DSM ...   103   2e-21
CP000771_660(CP000771|pid:none) Fervidobacterium nodosum Rt17-B1...   100   4e-20
CP001110_431(CP001110|pid:none) Pelodictyon phaeoclathratiforme ...    96   8e-19
(A1BDK8) Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA...    96   8e-19
(A6TWK7) RecName: Full=Glutamyl-tRNA synthetase;          EC=6.1...    94   2e-18
CP001034_168(CP001034|pid:none) Natranaerobius thermophilus JW/N...    94   2e-18
CP001251_1210(CP001251|pid:none) Dictyoglomus turgidum DSM 6724,...    94   3e-18
CP000607_1485(CP000607|pid:none) Chlorobium phaeovibrioides DSM ...    92   9e-18
CP001146_1116(CP001146|pid:none) Dictyoglomus thermophilum H-6-1...    92   1e-17

 psg: 0.76  gvh: 0.65  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VSK255 is nuc

>VSK255F.Seq
CTGATAAATNAAAATTATCAAAACGTCAAGGTGATGTTTCAGTTGATTCCTACATCTCAA
AAGGTTATTTACCAGAAGCATTAATTAATTTCGTTTCATTTTTAGGTTGGTCACCATCAA
CATCAACAACATCATCAACAACAACAACAACAACAACAACAACTGATCAAGAAACAAGTT
CAAAAGAAATATTTACACTTAAAGAATTGGTTGAATCATTTTCATTAGATGGTATTAATA
AGAGTGGTAGTGTTGTTAATATGGAGCGTTTAGATTGGTTAAATGTTAATCATATTAGAT
TACAAATTGATGATCCATTAAAACAAAATGAAATTTTATTAAAAATTAAAAATCAATTAT
TAGAATATTTTAATAATAATAATAATATAGATTTTAAAAAAGAAATTGATACAGAATATT
TATTAAAATCAATTAACTGTGTTAAAGAAAGAGTTCATTTAATTGAAGATTTTAATAACT
TATTAAAACCATTTTTTATTGATCAAATCGATTATACAACTGAAGAAGCAATTAAAATGA
AACAAAAAGTTTGGAA----------