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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSK254
Library VS
(Link to library)
Clone ID VSK254
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSK254P
(Link to Original site)
Representative DNA sequence 
>VSK254 (VSK254Q) /CSM/VS/VSK2-C/VSK254Q.Seq.d/
AATAAATTAAAATAAAATGACACTCGGAGGATTAAAACCAGAAGTACACGCCGCCAATGA
TGAAATTAGACAAGTCGTTGCCAAAGTTGCCGATGAACTCAAATCTAAATTAAACACCAC
TGAAGTCGAACCAGTTTCATACAAAACTCAACTCGTTGCTGGTACCAACTACTTTATCAA
AGTTAAGACCCCAGCTGGTTTTGCTCATGCTCGTGTCTACAAAGATTTACAACAAAACCA
TTCAGTCCACTCCGTCAAAGCTGATGGTATCACCGAAGAATCAGAAATTGTTTATTTCTA
AACTTTCAAGTGTATAATATATAAAACAATTGTTACTTTTATACTAAAATAAATAAAACA
CCTAAAAACAAAACATTACTTTATTTTTTTATTTAAAAAAAAAAXXXXXXXXXXAATAAA
TTAAAATAAAATGACACTCGGAGGATTAAAACCAGAAGTACACGCCGCCAATGATGAAAT
TAGACAAGTCGTTGCCAAAGTTGCCGATGAACTCAAATCTAAATTAAACACCACTGAAGT
CGAACCAGTTTCATACAAAACTCAACTCGTTGCTGGTACCAACTACTTTATCAAAGTTAA
GACCCCAGCTGGTTTTGCTCATGCTCGTGTCTACAAAGATTTACAACAAAACCATTCAGT
CCACTCCGTCAAAGCTGATGGTATCACCGAAGAATCAGAAATTGTTTATTTCTAAACTTT
CAAGTGTATAATATATAAAACAATTGTTACTTTTATACTAAAATAAATAAAACACCTAAA
AACAAAACATTA
sequence update 2001. 3.22
Translated Amino Acid sequence 
in*nkmtlgglkpevhaandeirqvvakvadelksklnttevepvsyktqlvagtnyfik
vktpagfaharvykdlqqnhsvhsvkadgiteeseivyf*tfkciiyktivtfilk*IKH
LKTKHYFIFLFKKK---

---NKLK*ndtrriktrstrrq**n*tsrcqscr*tqi*ikhh*srtsfiqnstrcwyql
lyqs*dpswfcscsclqrfttkpfsplrqs*wyhrrirnclflnfqvyni*nncyfytki
nktpknktl
Translated Amino Acid sequence (All Frames) 
Frame A:
nklk*ndtrriktrstrrq**n*tsrcqscr*tqi*ikhh*srtsfiqnstrcwyqllyq
s*dpswfcscsclqrfttkpfsplrqs*wyhrrirnclflnfqvyni*nncyfytkinkt
pknktllyffi*kk---

---NKLK*ndtrriktrstrrq**n*tsrcqscr*tqi*ikhh*srtsfiqnstrcwyql
lyqs*dpswfcscsclqrfttkpfsplrqs*wyhrrirnclflnfqvyni*nncyfytki
nktpknktl

Frame B:
in*nkmtlgglkpevhaandeirqvvakvadelksklnttevepvsyktqlvagtnyfik
vktpagfaharvykdlqqnhsvhsvkadgiteeseivyf*tfkciiyktivtfilk*IKH
LKTKHYFIFLFKKK---

---in*nkmtlgglkpevhaandeirqvvakvadelksklnttevepvsyktqlvagtny
fikvktpagfaharvykdlqqnhsvhsvkadgiteeseivyf*tfkciiyktivtfilk*
ikhlktkh

Frame C:
*ikik*hsed*nqkytppmmkldkslpklpmnsnln*tplksnqfhtklnsllvpttlsk
lrpqlvllmlvstkiynktiqstpsklmvspknqklfisklssv*yikqlllly*nk*nt
*kqnitlffylkkk---

---*ikik*hsed*nqkytppmmkldkslpklpmnsnln*tplksnqfhtklnsllvptt
lsklrpqlvllmlvstkiynktiqstpsklmvspknqklfisklssv*yikqlllly*nk
*nt*kqni
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK254 (VSK254Q) /CSM/VS/VSK2-C/VSK254Q.Seq.d/                        682   0.0
VSF637 (VSF637Q) /CSM/VS/VSF6-B/VSF637Q.Seq.d/                        682   0.0
VSF605 (VSF605Q) /CSM/VS/VSF6-A/VSF605Q.Seq.d/                        682   0.0
VSF202 (VSF202Q) /CSM/VS/VSF2-A/VSF202Q.Seq.d/                        682   0.0
VSE231 (VSE231Q) /CSM/VS/VSE2-B/VSE231Q.Seq.d/                        682   0.0
VSB135 (VSB135Q) /CSM/VS/VSB1-B/VSB135Q.Seq.d/                        682   0.0
SSK228 (SSK228Q) /CSM/SS/SSK2-B/SSK228Q.Seq.d/                        682   0.0
SSI815 (SSI815Q) /CSM/SS/SSI8-A/SSI815Q.Seq.d/                        682   0.0
VSH506 (VSH506Q) /CSM/VS/VSH5-A/VSH506Q.Seq.d/                        674   0.0
VHI872 (VHI872Q) /CSM/VH/VHI8-C/VHI872Q.Seq.d/                        674   0.0
own update 2003. 1.10
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M27786|M27786.1 Synthetic human inhibitorily active MS-2 pol-stefin B fusion protein gene, complete cds.    52  0.011  1
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    36  0.019  8
BQ633139|BQ633139.1 PfESToab38c04.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5', mRNA sequence.    36  0.024  2
BU496524|BU496524.1 PfESToab51c12.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5', mRNA sequence.    36  0.026  2
BU496772|BU496772.1 PfESToab58f06.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5', mRNA sequence.    36  0.027  2
AX348891|AX348891.1 Sequence 349 from Patent WO0202807.           34  0.081  4
AX252126|AX252126.1 Sequence 387 from Patent WO0168911.           34  0.081  4
AC097480|AC097480.3 Homo sapiens chromosome 4 clone RP11-123O22, complete sequence.    34  0.12  6
AX458577|AX458577.1 Sequence 123 from Patent WO0246454.           36  0.13  4
AX344551|AX344551.1 Sequence 2 from Patent WO0200932.             32  0.19  10
dna update 2003. 7.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q65YR8) RecName: Full=Cystatin-A1;  &AB189918_1(AB189918|pid:no...   187   2e-46
(Q65YR7) RecName: Full=Cystatin-A2;  &AB189919_1(AB189919|pid:no...    73   1e-11
(Q5R1U3) RecName: Full=Cystatin-A3; AltName: Full=VSA745;  &AB18...    64   5e-09
AC116987_9(AC116987|pid:none) Dictyostelium discoideum chromosom...    60   7e-08
EU979494_1(EU979494|pid:none) Ornithoctonus huwena clone HWEc85 ...    57   5e-07
(P01040) RecName: Full=Cystatin-A; AltName: Full=Cystatin-AS; Al...    57   5e-07
DQ646915_1(DQ646915|pid:none) Boophilus microplus putative intra...    55   2e-06
BC146444_1(BC146444|pid:none) Synthetic construct Mus musculus c...    54   7e-06
BC166959_1(BC166959|pid:none) Xenopus tropicalis cDNA clone MGC:...    53   1e-05
(P80416) RecName: Full=Cystatin-A; AltName: Full=Cystatin-AS; Al...    52   2e-05
protein update 2009. 3.26
PSORT 

 psg: 0.80  gvh: 0.08  alm: 0.66  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.24  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VSK254 is nuc
5' end seq. ID VSK254F
5' end seq. 
>VSK254F.Seq
AATAAATTAAAATAAAATGACACTCGGAGGATTAAAACCAGAAGTACACGCCGCCAATGA
TGAAATTAGACAAGTCGTTGCCAAAGTTGCCGATGAACTCAAATCTAAATTAAACACCAC
TGAAGTCGAACCAGTTTCATACAAAACTCAACTCGTTGCTGGTACCAACTACTTTATCAA
AGTTAAGACCCCAGCTGGTTTTGCTCATGCTCGTGTCTACAAAGATTTACAACAAAACCA
TTCAGTCCACTCCGTCAAAGCTGATGGTATCACCGAAGAATCAGAAATTGTTTATTTCTA
AACTTTCAAGTGTATAATATATAAAACAATTGTTACTTTTATACTAAAATAAATAAAACA
CCTAAAAACAAAACATTACTTTATTTTTTTATTTAAAAAAAAAA----------
Length of 5' end seq. 404
3' end seq. ID VSK254Z
3' end seq. 
>VSK254Z.Seq
----------AATAAATTAAAATAAAATGACACTCGGAGGATTAAAACCAGAAGTACACG
CCGCCAATGATGAAATTAGACAAGTCGTTGCCAAAGTTGCCGATGAACTCAAATCTAAAT
TAAACACCACTGAAGTCGAACCAGTTTCATACAAAACTCAACTCGTTGCTGGTACCAACT
ACTTTATCAAAGTTAAGACCCCAGCTGGTTTTGCTCATGCTCGTGTCTACAAAGATTTAC
AACAAAACCATTCAGTCCACTCCGTCAAAGCTGATGGTATCACCGAAGAATCAGAAATTG
TTTATTTCTAAACTTTCAAGTGTATAATATATAAAACAATTGTTACTTTTATACTAAAAT
AAATAAAACACCTAAAAACAAAACATTA
Length of 3' end seq. 378
Connected seq. ID VSK254P
Connected seq. 
>VSK254P.Seq
AATAAATTAAAATAAAATGACACTCGGAGGATTAAAACCAGAAGTACACGCCGCCAATGA
TGAAATTAGACAAGTCGTTGCCAAAGTTGCCGATGAACTCAAATCTAAATTAAACACCAC
TGAAGTCGAACCAGTTTCATACAAAACTCAACTCGTTGCTGGTACCAACTACTTTATCAA
AGTTAAGACCCCAGCTGGTTTTGCTCATGCTCGTGTCTACAAAGATTTACAACAAAACCA
TTCAGTCCACTCCGTCAAAGCTGATGGTATCACCGAAGAATCAGAAATTGTTTATTTCTA
AACTTTCAAGTGTATAATATATAAAACAATTGTTACTTTTATACTAAAATAAATAAAACA
CCTAAAAACAAAACATTACTTTATTTTTTTATTTAAAAAAAAAA----------AATAAA
TTAAAATAAAATGACACTCGGAGGATTAAAACCAGAAGTACACGCCGCCAATGATGAAAT
TAGACAAGTCGTTGCCAAAGTTGCCGATGAACTCAAATCTAAATTAAACACCACTGAAGT
CGAACCAGTTTCATACAAAACTCAACTCGTTGCTGGTACCAACTACTTTATCAAAGTTAA
GACCCCAGCTGGTTTTGCTCATGCTCGTGTCTACAAAGATTTACAACAAAACCATTCAGT
CCACTCCGTCAAAGCTGATGGTATCACCGAAGAATCAGAAATTGTTTATTTCTAAACTTT
CAAGTGTATAATATATAAAACAATTGTTACTTTTATACTAAAATAAATAAAACACCTAAA
AACAAAACATTA
Length of connected seq. 782
Full length Seq ID -
Full length Seq. -
Length of full length seq. -