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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSK250
Library VS
(Link to library)
Clone ID VSK250
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13129-1
Original site URL
Representative seq. ID VSK250P
(Link to Original site)
Representative DNA sequence 
>VSK250 (VSK250Q) /CSM/VS/VSK2-C/VSK250Q.Seq.d/
ATCGGATGGGTAATGGGACGAATTTTATGGAGATTTTCTTATAAATGGAGTTATCCTACA
ATAGGAGATATTGAAATATTTAAAAATTTTACAGCATATAATCAAATAATACATGGAACA
GTTATTGAAATCGTTTGGACATTAATTCCAACAGTAATATTATATTTAATAGCAATTCCA
TCCTTTACATTATTATATGCGATGGATGAAATTATTAATCCAACAGTAACAATCAAAATT
ATAGGTCATCAATGGTATTGGAGTTATGAATATGGAGATAATGCAAGTAATTTAATAGAA
TTTGATAGTTATATGGTTTATGAACGAGATTTAGCAGAAGGACAACTAAGATTATTAGAA
GTAGATAATGCAATGGTAGTTCCAGTAAAAACACATATTAGATTAATAATAACATCAGGA
GATGTTTTACATAGTTGGGCGATTCCATCATTTGGAATAAAAGTAGATGCCGTACXXXXX
XXXXXTAAGCAATGGGCAGCAGGTAACGGCATTAAAGGCTTTACTGAATAACATAATAGA
ATACAGAGACACTCATGCAATATCCGAATTGGAAGGGAAAAAGCTACAACAAGTAGCGGA
TGAACTAAGACGGTTATATGAGAGGGAAGATCAAATCGAAAAGGAAATAAATAAAATCTT
AAGTAAGGCCTCTAAAAGAGCAGGGGGAGGTTGGATAAAAGAAGAATAATGAAACAAAAT
TTAAAAAAAACGGTTTTAAAAGCAGGATGGATACCAGGAACGGAAACAGCTATTGCAAAA
ATGGTTATAAGTATTTGTGTTATCTTTGCAGGTTATTATACATTATACTCTGCCTACGCA
GAGTATAACCAAATTGTAGAACAAAATGCACAAGCTGTTCTAAGCGAAGTAACAAGTAGT
AATATGCCCGTACCGCCATTAGAGAGTTTAACTGATATTTTAGTAGCATCTGAAAAATTA
TCTCCGCAAAGTAAAGTAAATTTTTTATTAGAGCATCAAACAAAGATAATCTCCTTACTA
AAGGGCTGTTGGGGGATACCTTATCTCCCGATCAAAAAGAGAATTTGAAGGAAATTGTAA
ATTTTTTAACAAAAAGAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
IGWVMGRILWRFSYKWSYPTIGDIEIFKNFTAYNQIIHGTVIEIVWTLIPTVILYLIAIP
SFTLLYAMDEIINPTVTIKIIGHQWYWSYEYGDNASNLIEFDSYMVYERDLAEGQLRLLE
VDNAMVVPVKTHIRLIITSGDVLHSWAIPSFGIKVDAV---

---SNGQQVTALKALLNNIIEYRDTHAISELEGKKLQQVADELRRLYEREDQIEKEINKI
LSKASKRAGGGWIKEE**nki*kkrf*kqdgyqerkqllqkwl*vfvlslqviihytlpt
qsitkl*nkmhklf*ak*qvvicpyrh*rv*lif**hlknylrkvk*ify*sikqr*spy
*ravggylisrskrefegnckffnkk
Translated Amino Acid sequence (All Frames) 
Frame A:
IGWVMGRILWRFSYKWSYPTIGDIEIFKNFTAYNQIIHGTVIEIVWTLIPTVILYLIAIP
SFTLLYAMDEIINPTVTIKIIGHQWYWSYEYGDNASNLIEFDSYMVYERDLAEGQLRLLE
VDNAMVVPVKTHIRLIITSGDVLHSWAIPSFGIKVDAV---

---*amgsr*rh*rly*it**ntetlmqypnwkgksynk*rmn*dgymrgkikskrk*ik
s*vrplkeqgevg*kknnetkfkkngfksrmdtrngnsyckngykylcylcrllyiilcl
rrv*pncrtkctscskrsnk**yartairefn*yfssi*kiisak*skffirasnkdnll
tkgllgdtlspdqkenlkeivnfltkr

Frame B:
sdg*wdefygdflingvilq*eilkylkilqhiik*ymeqllksfgh*fqq*yyi**qfh
plhyymrwmklliqq*qskl*vingigvmnmeimqvi**nliviwfmnei*qkdn*dy*k
*imqw*fq*khild***hqemfyivgrfhhle*k*mpy---

---kqwaagngikgfte*hnriqrhscnirigrekattssg*tktvi*egrsnrkgnk*n
lk*gl*ksrgrldkrrimkqnlkktvlkagwipgtetaiakmvisicvifagyytlysay
aeynqiveqnaqavlsevtssnmpvpplesltdilvaseklspqskvnfllehqtkiisl
lkgcwgipylpikkri*rkl*if*qke

Frame C:
rmgngtnfmeifl*melsynrry*ni*kfysi*snntwnsy*nrldinsnsniifnsnsi
lyiiicdg*ny*snsnnqnyrssmvlel*iwr*ck*fnri**lygl*trfsrrttkiirs
r*cngsssknty*innnirrcft*lgdsiiwnksrcr---

---SNGQQVTALKALLNNIIEYRDTHAISELEGKKLQQVADELRRLYEREDQIEKEINKI
LSKASKRAGGGWIKEE**nki*kkrf*kqdgyqerkqllqkwl*vfvlslqviihytlpt
qsitkl*nkmhklf*ak*qvvicpyrh*rv*lif**hlknylrkvk*ify*sikqr*spy
*ravggylisrskrefegnckffnkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK250 (VSK250Q) /CSM/VS/VSK2-C/VSK250Q.Seq.d/                       2070   0.0
MIT-GENOME (MIT-GENOMEQ) /CSM/OTHERS/MIT-GENOMEQ.Seq.d/              1023   0.0
VFN289 (VFN289Q) /CSM/VF/VFN2-D/VFN289Q.Seq.d/                       1021   0.0
VHP858 (VHP858Q) /CSM/VH/VHP8-C/VHP858Q.Seq.d/                        731   0.0
VHN576 (VHN576Q) /CSM/VH/VHN5-D/VHN576Q.Seq.d/                        684   0.0
VHB602 (VHB602Q) /CSM/VH/VHB6-A/VHB602Q.Seq.d/                        684   0.0
VHJ852 (VHJ852Q) /CSM/VH/VHJ8-C/VHJ852Q.Seq.d/                        662   0.0
VHI649 (VHI649Q) /CSM/VH/VHI6-C/VHI649Q.Seq.d/                        615   e-175
VSE881 (VSE881Q) /CSM/VS/VSE8-D/VSE881Q.Seq.d/                        543   e-153
SHH491 (SHH491Q) /CSM/SH/SHH4-D/SHH491Q.Seq.d/                        529   e-149
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AB000109|AB000109.1 Dictyostelium discoideum mitochondrial DNA, complete sequence.   488  0.0  11
X81884|X81884.1 D.discoideum mitochondrial cox1 gene and cox2 gene.   488  0.0  2
D50297|D50297.1 Dictyostelium discoideum mitochondrial DNA for cytochrome oxidase subunit 1 and subunit 2, ai4, ai3, ai2b, ai2a,complete cds.   488  0.0  2
AF086692|AF086692.1 Aphanomyces euteiches cytochrome c oxidase subunit II (cox2) gene, partial cds; mitochondrial gene for mitochondrial product.    52  5e-22  4
AY129209|AY129209.1 Phytophthora megakarya isolate 328 cytochrome c oxidase subunit II (coxII) gene, partial cds; mitochondrial gene for mitochondrial product.    52  1e-21  5
AY129196|AY129196.1 Phytophthora fragariae var. rubi isolate 397 cytochrome c oxidase subunit II (coxII) gene, partial cds; mitochondrial gene for mitochondrial product.    52  7e-20  5
AY129177|AY129177.1 Phytophthora boehmeriae isolate 325 cytochrome c oxidase subunit II (coxII) gene, partial cds; mitochondrial gene for mitochondrial product.    50  2e-19  5
AF086698|AF086698.1 Peronophythora litchii cytochrome c oxidase subunit II (cox2) gene, partial cds; mitochondrial gene for mitochondrial product.    52  3e-19  5
AY129184|AY129184.1 Phytophthora citricola isolate SB2084 cytochrome c oxidase subunit II (coxII) gene, partial cds; mitochondrial gene for mitochondrial product.    46  9e-19  5
AY129183|AY129183.1 Phytophthora citricola isolate Cr-4 cytochrome c oxidase subunit II (coxII) gene, partial cds; mitochondrial gene for mitochondrial product.    46  9e-19  5
dna update 2003. 7.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X81884_2(X81884|pid:none) D.discoideum mitochondrial cox1 gene a...   327   3e-88
(O21042) RecName: Full=Cytochrome c oxidase subunit 1+2;        ...   327   3e-88
DQ336395_6(DQ336395|pid:none) Dictyostelium citrinum mitochondri...   320   4e-86
EU275727_7(EU275727|pid:none) Dictyostelium fasciculatum strain ...   270   6e-71
AY700145_35(AY700145|pid:none) Polysphondylium pallidum mitochon...   268   2e-70
EU275726_6(EU275726|pid:none) Polysphondylium pallidum strain PN...   268   3e-70
AY267353_29(AY267353|pid:none) Chara vulgaris mitochondrion, com...   192   2e-47
AF114794_21(AF114794|pid:none) Porphyra purpurea mitochondrion, ...   190   6e-47
AF494279_24(AF494279|pid:none) Chaetosphaeridium globosum mitoch...   190   8e-47
AF116778_1(AF116778|pid:none) Tetraselmis aff. maculata cytochro...   189   2e-46
protein update 2009. 3.26
PSORT 

 psg: 0.72  gvh: 0.51  alm: 0.26  top: 0.53  tms: 0.07  mit: 0.36  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.37  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 16.0 %: Golgi
	 12.0 %: nuclear
	 12.0 %: cytoplasmic
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VSK250 is end
5' end seq. ID VSK250F
5' end seq. 
>VSK250F.Seq
ATCGGATGGGTAATGGGACGAATTTTATGGAGATTTTCTTATAAATGGAGTTATCCTACA
ATAGGAGATATTGAAATATTTAAAAATTTTACAGCATATAATCAAATAATACATGGAACA
GTTATTGAAATCGTTTGGACATTAATTCCAACAGTAATATTATATTTAATAGCAATTCCA
TCCTTTACATTATTATATGCGATGGATGAAATTATTAATCCAACAGTAACAATCAAAATT
ATAGGTCATCAATGGTATTGGAGTTATGAATATGGAGATAATGCAAGTAATTTAATAGAA
TTTGATAGTTATATGGTTTATGAACGAGATTTAGCAGAAGGACAACTAAGATTATTAGAA
GTAGATAATGCAATGGTAGTTCCAGTAAAAACACATATTAGATTAATAATAACATCAGGA
GATGTTTTACATAGTTGGGCGATTCCATCATTTGGAATAAAAGTAGATGCCGTAC-----
-----
Length of 5' end seq. 475
3' end seq. ID VSK250Z
3' end seq. 
>VSK250Z.Seq
----------TAAGCAATGGGCAGCAGGTAACGGCATTAAAGGCTTTACTGAATAACATA
ATAGAATACAGAGACACTCATGCAATATCCGAATTGGAAGGGAAAAAGCTACAACAAGTA
GCGGATGAACTAAGACGGTTATATGAGAGGGAAGATCAAATCGAAAAGGAAATAAATAAA
ATCTTAAGTAAGGCCTCTAAAAGAGCAGGGGGAGGTTGGATAAAAGAAGAATAATGAAAC
AAAATTTAAAAAAAACGGTTTTAAAAGCAGGATGGATACCAGGAACGGAAACAGCTATTG
CAAAAATGGTTATAAGTATTTGTGTTATCTTTGCAGGTTATTATACATTATACTCTGCCT
ACGCAGAGTATAACCAAATTGTAGAACAAAATGCACAAGCTGTTCTAAGCGAAGTAACAA
GTAGTAATATGCCCGTACCGCCATTAGAGAGTTTAACTGATATTTTAGTAGCATCTGAAA
AATTATCTCCGCAAAGTAAAGTAAATTTTTTATTAGAGCATCAAACAAAGATAATCTCCT
TACTAAAGGGCTGTTGGGGGATACCTTATCTCCCGATCAAAAAGAGAATTTGAAGGAAAT
TGTAAATTTTTTAACAAAAAGAA
Length of 3' end seq. 613
Connected seq. ID VSK250P
Connected seq. 
>VSK250P.Seq
ATCGGATGGGTAATGGGACGAATTTTATGGAGATTTTCTTATAAATGGAGTTATCCTACA
ATAGGAGATATTGAAATATTTAAAAATTTTACAGCATATAATCAAATAATACATGGAACA
GTTATTGAAATCGTTTGGACATTAATTCCAACAGTAATATTATATTTAATAGCAATTCCA
TCCTTTACATTATTATATGCGATGGATGAAATTATTAATCCAACAGTAACAATCAAAATT
ATAGGTCATCAATGGTATTGGAGTTATGAATATGGAGATAATGCAAGTAATTTAATAGAA
TTTGATAGTTATATGGTTTATGAACGAGATTTAGCAGAAGGACAACTAAGATTATTAGAA
GTAGATAATGCAATGGTAGTTCCAGTAAAAACACATATTAGATTAATAATAACATCAGGA
GATGTTTTACATAGTTGGGCGATTCCATCATTTGGAATAAAAGTAGATGCCGTAC-----
-----TAAGCAATGGGCAGCAGGTAACGGCATTAAAGGCTTTACTGAATAACATAATAGA
ATACAGAGACACTCATGCAATATCCGAATTGGAAGGGAAAAAGCTACAACAAGTAGCGGA
TGAACTAAGACGGTTATATGAGAGGGAAGATCAAATCGAAAAGGAAATAAATAAAATCTT
AAGTAAGGCCTCTAAAAGAGCAGGGGGAGGTTGGATAAAAGAAGAATAATGAAACAAAAT
TTAAAAAAAACGGTTTTAAAAGCAGGATGGATACCAGGAACGGAAACAGCTATTGCAAAA
ATGGTTATAAGTATTTGTGTTATCTTTGCAGGTTATTATACATTATACTCTGCCTACGCA
GAGTATAACCAAATTGTAGAACAAAATGCACAAGCTGTTCTAAGCGAAGTAACAAGTAGT
AATATGCCCGTACCGCCATTAGAGAGTTTAACTGATATTTTAGTAGCATCTGAAAAATTA
TCTCCGCAAAGTAAAGTAAATTTTTTATTAGAGCATCAAACAAAGATAATCTCCTTACTA
AAGGGCTGTTGGGGGATACCTTATCTCCCGATCAAAAAGAGAATTTGAAGGAAATTGTAA
ATTTTTTAACAAAAAGAA
Length of connected seq. 1088
Full length Seq ID -
Full length Seq. -
Length of full length seq. -