>VSK205 (VSK205Q) /CSM/VS/VSK2-A/VSK205Q.Seq.d/
XXXXXXXXXXTAATGGTNGGTTTANATNNTGNTGGTAAANNCACCATTTTATACAAANTT
AAATTAGGTNAAATTGTTANTACAATTNCAANCATTGGTTTCAATGTNGAAANTGTNGAA
TTCAAAAACATTAANTTCATTGTATGGGATGTTGGTGGTCAAGATANAATTNGTNCATTA
TGNAGATATTATTTCCNAAATACTNAAGGTNTTATNTTTGTTGTTGATTCAAACGATAGA
GAAAGAATTCAANAAGCTTGTGATGAACTCACTAAAATGNTCAATGAAGATNAATTACGT
GATGCTGTTTTATTAGTTTTCTGTAACAAACAAGATCTTCCAAATGCCATGAGTGTCGNT
GAAGTTACNGATAAATTAAATNTCCACTCTCTCCGTTCAAGAAAATGGTACATACAGTCA
ACTTGTGCCANCAGTGGTNATGGTTTATATGAAGGTTTAGACTGGCT

---MVGLXXXGKXTILYKXKLGXIVXTIXXIGFNVEXVEFKNIXFIVWDVGGQDXIXXLX
RYYFXNTXGXXFVVDSNDRERIQXACDELTKMXNEDXLRDAVLLVFCNKQDLPNAMSVXE
VTDKLNXHSLRSRKWYIQSTCAXSGXGLYEGLDW

Frame A:
---ngxfxxxw*xhhfiqx*irxncxynxxhwfqcxxcxiqkh*xhcmgcwwsrxnxxim
xilfpkyxrxyxcc*fkr*rknsxsl**th*nxq*rxit*ccfisfl*qtrsskchecr*
syx*ikxplspfkkmvhtvnlcxqwxwfi*rfrla


Frame B:
---MVGLXXXGKXTILYKXKLGXIVXTIXXIGFNVEXVEFKNIXFIVWDVGGQDXIXXLX
RYYFXNTXGXXFVVDSNDRERIQXACDELTKMXNEDXLRDAVLLVFCNKQDLPNAMSVXE
VTDKLNXHSLRSRKWYIQSTCAXSGXGLYEGLDW


Frame C:
---wxvxxxxvxxpfytxln*vkllxqxqxlvsmxkxxnsktlxslygmlvvkixxvhyx
diisxilkvlxllliqtiekefxklvmnslkxsmkxnyvmlfy*fsvtnkifqmp*vsxk
lxin*xstlsvqengtysqlvpxvvmvymkv*tg

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK205 (VSK205Q) /CSM/VS/VSK2-A/VSK205Q.Seq.d/                        716   0.0
SSL154 (SSL154Q) /CSM/SS/SSL1-C/SSL154Q.Seq.d/                        700   0.0
SSL113 (SSL113Q) /CSM/SS/SSL1-A/SSL113Q.Seq.d/                        700   0.0
SSK773 (SSK773Q) /CSM/SS/SSK7-D/SSK773Q.Seq.d/                        700   0.0
SSK216 (SSK216Q) /CSM/SS/SSK2-A/SSK216Q.Seq.d/                        700   0.0
SSJ475 (SSJ475Q) /CSM/SS/SSJ4-D/SSJ475Q.Seq.d/                        700   0.0
SSI674 (SSI674Q) /CSM/SS/SSI6-D/SSI674Q.Seq.d/                        700   0.0
SSI502 (SSI502Q) /CSM/SS/SSI5-A/SSI502Q.Seq.d/                        700   0.0
SSI437 (SSI437Q) /CSM/SS/SSI4-B/SSI437Q.Seq.d/                        700   0.0
SSG749 (SSG749Q) /CSM/SS/SSG7-C/SSG749Q.Seq.d/                        700   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AJ000063|AJ000063.1 Dictyostelium discoideum mRNA for ADP-ribosylation factor 1.   700  0.0  1
BG225964|BG225964.1 kp44b08.y1 TBN95TM-SSFH Strongyloides stercoralis cDNA 5' similar to gb:M36341 ADP-RIBOSYLATION FACTOR 4 (HUMAN);, mRNA sequence.    48  2e-16  4
AB001051|AB001051.1 Dugesia japonica mRNA for ADP-ribosylation factor, complete cds.    92  9e-16  2
AI187662|AI187662.1 EST485 Manduca sexta male antennae Uni-ZAP XR library Manduca sexta cDNA clone pMsmaD225 5' similar to ADP-ribosylation factor 1, mRNA sequence.    88  1e-13  1
CA898547|CA898547.1 PCEP02999 Scarlet Runner Bean Embryo-Proper Region Phaseolus coccineus cDNA 5' similar to ADP-ribosylation factor, mRNA sequence.    60  1e-13  2
CA907616|CA907616.1 PCSC17404 Scarlet Runner Bean Suspensor Region TriplEx2 Phaseolus coccineus cDNA 5' similar to ADP-ribosylation factor, mRNA sequence.    60  1e-13  2
CA907618|CA907618.1 PCSC19544 Scarlet Runner Bean Suspensor Region TriplEx2 Phaseolus coccineus cDNA 5' similar to ADP-ribosylation factor, mRNA sequence.    60  1e-13  2
BE920667|BE920667.1 EST424436 potato leaves and petioles Solanum tuberosum cDNA clone cSTB10K15 5' sequence, mRNA sequence.    60  5e-13  2
BG097224|BG097224.1 EST461743 potato leaves and petioles Solanum tuberosum cDNA clone cSTB46O3 5' sequence, mRNA sequence.    60  5e-13  2
BG136653|BG136653.1 EST477095 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP3C22 5' sequence, mRNA sequence.    60  5e-13  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O00909) RecName: Full=ADP-ribosylation factor 1;  &AJ000063_1(A...   244   5e-64
AP003570_13(AP003570|pid:none) Oryza sativa Japonica Group genom...   219   2e-56
(Q06396) RecName: Full=ADP-ribosylation factor 1; AltName: Full=...   219   2e-56
(P51821) RecName: Full=ADP-ribosylation factor 1;  &S66337(S6633...   219   2e-56
AM454724_7(AM454724|pid:none) Vitis vinifera contig VV78X229598....   219   3e-56
EF677861_1(EF677861|pid:none) Picea sitchensis clone WS02776_J02...   218   4e-56
BT052802_1(BT052802|pid:none) Medicago truncatula clone MTYFH_FI...   218   5e-56
AC135465_4(AC135465|pid:none) Medicago truncatula clone mth2-24h...   218   5e-56
AF108891_1(AF108891|pid:none) Capsicum annuum ADP-ribosylation f...   218   5e-56
AC009398_7(AC009398|pid:none) Genomic sequence for Arabidopsis t...   218   5e-56

 psg: 0.64  gvh: 0.40  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal

>> prediction for VSK205 is cyt

>VSK205Z.Seq
----------TAATGGTNGGTTTANATNNTGNTGGTAAANNCACCATTTTATACAAANTT
AAATTAGGTNAAATTGTTANTACAATTNCAANCATTGGTTTCAATGTNGAAANTGTNGAA
TTCAAAAACATTAANTTCATTGTATGGGATGTTGGTGGTCAAGATANAATTNGTNCATTA
TGNAGATATTATTTCCNAAATACTNAAGGTNTTATNTTTGTTGTTGATTCAAACGATAGA
GAAAGAATTCAANAAGCTTGTGATGAACTCACTAAAATGNTCAATGAAGATNAATTACGT
GATGCTGTTTTATTAGTTTTCTGTAACAAACAAGATCTTCCAAATGCCATGAGTGTCGNT
GAAGTTACNGATAAATTAAATNTCCACTCTCTCCGTTCAAGAAAATGGTACATACAGTCA
ACTTGTGCCANCAGTGGTNATGGTTTATATGAAGGTTTAGACTGGCT