>VSK190 (VSK190Q) /CSM/VS/VSK1-D/VSK190Q.Seq.d/
GTTTAAAAAGAATAATATTGTATATTTATTAAAATTGTACATTATTATTATTATAGAAAG
AGAAAAAAGCAATAAATAACTTTAATTCATACAAACAAAAACAACAACATATATATATAA
ACATTAAAAGATGGGTTTATTTGGTGGTAGAAAACATGGTGGTTTATTCACTTTCAATAA
AGATGATGGTTATTTAGAAGCTATCTTAAGAGGTTTCAAAAAAGGTATCCTCTCAAGAGC
CGATTACAATAATTTATGTCAATGCGATAATTTAGAAGATATGAAAATGCATTTCATTTC
AACAGATTATGGTGATTTCCCTCAGGTGAACCATCACCAATTCATACAACCACCATTGCA
GAGAAAGCAACAGGTAAATTAGTAAGTGAATTCAACCACATTAGAAATCAAGCAGTCGAA
CCACTTTCAACTTTTATGGATTTCATTTCATATGGTTATATGATTGATAACCGTCGTTTT
ATTAATCACTGGTACTCTCCACGAAAGAGATATCTCTGAATTGGTCGATAAATGTCACCC
ATTAXXXXXXXXXX

fkknnivyllklyiiiiiereksnk*l*fiqtktttyiykh*KMGLFGGRKHGGLFTFNK
DDGYLEAILRGFKKGILSRADYNNLCQCDNLEDMKMHFISTDYGDFPQVNHHQFIQPPLQ
RKQQVN**vnsttleikqsnhfqllwisfhmvi*litvvllitgtlherdiselvdkchp
---

Frame A:
v*ke*ycifikivhyyyyrkrkkq*itlihtnknnniyi*tlkdgfiww*ktwwfihfq*
r*wlfrsylkrfqkryplksrlq*fmsmr*frryenafhfnrlw*fpsgepspihtttia
ekatgklvsefnhirnqaveplstfmdfisygymidnrrfinhwysprkryl*igr*msp
i---


Frame B:
fkknnivyllklyiiiiiereksnk*l*fiqtktttyiykh*KMGLFGGRKHGGLFTFNK
DDGYLEAILRGFKKGILSRADYNNLCQCDNLEDMKMHFISTDYGDFPQVNHHQFIQPPLQ
RKQQVN**vnsttleikqsnhfqllwisfhmvi*litvvllitgtlherdiselvdkchp
---


Frame C:
lkriilyiy*nctllll*kekkainnfnsykqkqqhiyinikrwvylvvenmvvyslsik
mmvi*kls*evskkvssqepitiiyvnaii*ki*kcisfqqimvislr*titnsynhhcr
esnr*isk*iqph*ksssrttfnfygfhfiwlyd**psfy*slvlstkeislnwsinvth
---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK190 (VSK190Q) /CSM/VS/VSK1-D/VSK190Q.Seq.d/                       1078   0.0
VSJ112 (VSJ112Q) /CSM/VS/VSJ1-A/VSJ112Q.Seq.d/                       1043   0.0
VSF718 (VSF718Q) /CSM/VS/VSF7-A/VSF718Q.Seq.d/                       1041   0.0
VHK694 (VHK694Q) /CSM/VH/VHK6-D/VHK694Q.Seq.d/                       1041   0.0
VFN487 (VFN487Q) /CSM/VF/VFN4-D/VFN487Q.Seq.d/                       1041   0.0
VFL485 (VFL485Q) /CSM/VF/VFL4-D/VFL485Q.Seq.d/                       1041   0.0
VFK347 (VFK347Q) /CSM/VF/VFK3-B/VFK347Q.Seq.d/                       1041   0.0
VFG596 (VFG596Q) /CSM/VF/VFG5-D/VFG596Q.Seq.d/                       1041   0.0
VFE465 (VFE465Q) /CSM/VF/VFE4-C/VFE465Q.Seq.d/                       1041   0.0
VFC648 (VFC648Q) /CSM/VF/VFC6-B/VFC648Q.Seq.d/                       1041   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U13150|U13150.1 Dictyostelium discoideum vacuolar ATPase subunit DVA41 mRNA, complete cds.   634  0.0  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   551  0.0  5
AC131729|AC131729.3 Mus musculus chromosome UNK clone RP23-125B15, WORKING DRAFT SEQUENCE, 1 unordered piece.    38  2e-04  7
AL929352|AL929352.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 2/4.    38  3e-04  5
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.          42  3e-04  6
AC025001|AC025001.3 Homo sapiens chromosome 1 clone RP11-196P5 map 1, WORKING DRAFT SEQUENCE, 18 unordered pieces.    52  0.001  4
AE001406|AE001406.2 Plasmodium falciparum 3D7 chromosome 2 section 43 of 73 of the complete sequence.    36  0.001  5
AL021155|AL021155.1 Homo sapiens DNA sequence from PAC 934G17 on chromosome 1p36.21.  Contains the alternatively spliced CLCN6 gene for chloride chanel  proteins CLC-6A (KIAA0046) -B, -C and -D, the alternatively spliced  NPPA gene coding for Atrial Natriuretic Factor ANF precursor (Atrial Natriuretic peptide ANP, Prepronatriodilatin), the NPPB gene for  Brain Natriuretic Protein BNP, and a pseudogene similar to SBF1 (and other Myotubularin-related protein genes). Contains ESTs, STSs and  the genomic marker D1S2740.    52  0.002  3
AC107860|AC107860.10 Mus musculus clone RP23-21P17, *** SEQUENCING IN PROGRESS ***, 2 ordered pieces.    40  0.004  6
BX249015|BX249015.1 Pinus pinaster xylem EST, clone PP010D09.     42  0.005  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A55016(A55016) lysosomal membrane protein DVA41 - slime mold (Di...   150   2e-35
(P54641) RecName: Full=V-type proton ATPase subunit d;          ...   150   2e-35
BC153472_1(BC153472|pid:none) Danio rerio ATPase, H+ transportin...    75   3e-21
AK300570_1(AK300570|pid:none) Homo sapiens cDNA FLJ54755 complet...    74   6e-20
FM992688_1201(FM992688|pid:none) Candida dubliniensis CD36 chrom...    68   1e-18
AM910994_408(AM910994|pid:none) Plasmodium knowlesi strain H chr...    61   3e-18
AX886058_1(AX886058|pid:none) Sequence 1921 from Patent EP1033401.     73   1e-17
AK089507_1(AK089507|pid:none) Mus musculus B6-derived CD11 +ve d...    64   2e-17
(Q80SY3) RecName: Full=V-type proton ATPase subunit d 2;        ...    64   2e-17
AB088358_1(AB088358|pid:none) Mus musculus Atp6Vod2 mRNA for pro...    64   2e-17

 psg: 0.81  gvh: 0.40  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VSK190 is nuc

>VSK190F.Seq
GTTTAAAAAGAATAATATTGTATATTTATTAAAATTGTACATTATTATTATTATAGAAAG
AGAAAAAAGCAATAAATAACTTTAATTCATACAAACAAAAACAACAACATATATATATAA
ACATTAAAAGATGGGTTTATTTGGTGGTAGAAAACATGGTGGTTTATTCACTTTCAATAA
AGATGATGGTTATTTAGAAGCTATCTTAAGAGGTTTCAAAAAAGGTATCCTCTCAAGAGC
CGATTACAATAATTTATGTCAATGCGATAATTTAGAAGATATGAAAATGCATTTCATTTC
AACAGATTATGGTGATTTCCCTCAGGTGAACCATCACCAATTCATACAACCACCATTGCA
GAGAAAGCAACAGGTAAATTAGTAAGTGAATTCAACCACATTAGAAATCAAGCAGTCGAA
CCACTTTCAACTTTTATGGATTTCATTTCATATGGTTATATGATTGATAACCGTCGTTTT
ATTAATCACTGGTACTCTCCACGAAAGAGATATCTCTGAATTGGTCGATAAATGTCACCC
ATTA----------