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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSK182
Library VS
(Link to library)
Clone ID VSK182
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12594-1
Original site URL
Representative seq. ID VSK182P
(Link to Original site)
Representative DNA sequence 
>VSK182 (VSK182Q) /CSM/VS/VSK1-D/VSK182Q.Seq.d/
GCAACAATCCCATCACCTCAATCTCAACTATTTCCATCAAGTTTATTTTTATCATCACCA
AATTCTGAAGAAACCATTACAATCTTTTTAATGATGTGTGTAAATAGTTTTAAAGCAAAT
AGTAATATATTAGATTTCATTTACACTGTAGAGAAATATCTTCGTCCAAACAAACCAATA
ATTAAAAGTGGTATGTTGGATTTGGATGAATTCAAAGAAAGAGAAGAAGCTTTTGAAAGC
CAATATGTTAAACAAAAAGAAAGAGATGATTTAAAGAGAATGGCTGAAAAGAAGAAACAA
GAAGAAACCAAAAAAGCTGGCTCACAACCAACTCCAAATGCATCATCATCAGCCAACAAT
TCCAAAGCTGCCAAGATTAAGGAAATTGAAGATAAAATTAGCAAATTACAAAAAGAACTT
GCCGATTTAAAAAAATAAATCAAAATCCCTGTGTTTTTATAAATAAATAAATAAATAAAT
ATATTAATATATAAATATATAAATAAAAAATAAAAAAAAAAXXXXXXXXXXCTGAAAAGA
AGAAACAAGAAGAAACCAAAAAAGCTGGCTCACAACCAACTCCAAATGCATCATCATCAG
CCAACAATTCCAAAGCTGCCAAGATTAAGGAAATTGAAGATAAAATTAGCAAATTACAAA
AAGAACTTGCCGATTTAAAAAAATAAATCAAAATCCCTGTGTTTTTATAAATAAATAAAT
AAATAAATATATTAATATATAAATATAAANAAAAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
qqshhlnlnyfhqvyfyhhqilkkplqsf**cv*ivlkqiviy*isftl*rnifvqtnq*
lkvvcwiwmnskkekkllkanmlnkkkemi*rewlkrrnkkkpkklahnqlqmhhhqpti
pklprlrklkiklanykknlpi*knkskslcfyk*ink*iy*yini*IKNKKK---

---EKKKQEETKKAGSQPTPNASSSANNSKAAKIKEIEDKISKLQKELADLKK*ikipvf
l*ink*iniliykykxk
Translated Amino Acid sequence (All Frames) 
Frame A:
atipspqsqlfpsslflsspnseetitiflmmcvnsfkansnildfiytvekylrpnkpi
iksgmldldefkereeafesqyvkqkerddlkrmaekkkqeetkkagsqptpnasssann
skaakikeiedkisklqkeladlkk*ikipvfl*ink*iniliykyinkk*kk---

---lkrrnkkkpkklahnqlqmhhhqptipklprlrklkiklanykknlpi*knkskslc
fyk*ink*iy*yinixkk

Frame B:
qqshhlnlnyfhqvyfyhhqilkkplqsf**cv*ivlkqiviy*isftl*rnifvqtnq*
lkvvcwiwmnskkekkllkanmlnkkkemi*rewlkrrnkkkpkklahnqlqmhhhqpti
pklprlrklkiklanykknlpi*knkskslcfyk*ink*iy*yini*IKNKKK---

---*keetrrnqkswlttnskciiisqqfqscqd*gn*r*n*qitkrtcrfkkinqnpcv
fink*inkyini*i*xk

Frame C:
nnpitsistisikfifiitkf*rnhynlfndvck*f*sk**yirfhlhcreissskqtnn
*kwyvgfg*iqrkrrsf*kpic*tkrkr*fkeng*keetrrnqkswlttnskciiisqqf
qscqd*gn*r*n*qitkrtcrfkkinqnpcvfink*inkyini*iyk*kikkk---

---EKKKQEETKKAGSQPTPNASSSANNSKAAKIKEIEDKISKLQKELADLKK*ikipvf
l*ink*iniliykykxk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK182 (VSK182Q) /CSM/VS/VSK1-D/VSK182Q.Seq.d/                        507   e-143
VSK372 (VSK372Q) /CSM/VS/VSK3-C/VSK372Q.Seq.d/                        303   2e-81
VSJ436 (VSJ436Q) /CSM/VS/VSJ4-B/VSJ436Q.Seq.d/                        303   2e-81
VSJ213 (VSJ213Q) /CSM/VS/VSJ2-A/VSJ213Q.Seq.d/                        303   2e-81
VSI712 (VSI712Q) /CSM/VS/VSI7-A/VSI712Q.Seq.d/                        303   2e-81
VSF706 (VSF706Q) /CSM/VS/VSF7-A/VSF706Q.Seq.d/                        303   2e-81
VSE206 (VSE206Q) /CSM/VS/VSE2-A/VSE206Q.Seq.d/                        303   2e-81
VSD852 (VSD852Q) /CSM/VS/VSD8-C/VSD852Q.Seq.d/                        303   2e-81
VSD770 (VSD770Q) /CSM/VS/VSD7-C/VSD770Q.Seq.d/                        303   2e-81
VSD470 (VSD470Q) /CSM/VS/VSD4-C/VSD470Q.Seq.d/                        303   2e-81
own update 2001.11.13
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF311910|AF311910.1 Dictyostelium discoideum F1F0-ATPase putative regulatory protein IF1 (if1) mRNA, complete cds; nuclear gene for mitochondrial product.   287  2e-97  2
AZ530018|AZ530018.1 ENTBT73TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36  0.026  3
BF015082|BF015082.1 kq64a10.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to contains element MER33 repetitive element ;, mRNA sequence.    36  0.032  2
BE579269|BE579269.1 kq25a04.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to contains element L1 repetitive element ;, mRNA sequence.    36  0.035  2
AZ681842|AZ681842.1 ENTIA03TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36  0.056  2
AZ550219|AZ550219.1 ENTEU85TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36  0.057  2
AZ673200|AZ673200.1 ENTIA73TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36  0.057  2
BH154707|BH154707.1 ENTRL79TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36  0.060  2
AZ546799|AZ546799.1 ENTEL88TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36  0.061  2
AZ541947|AZ541947.1 ENTDS75TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36  0.062  2
dna update 2003. 7.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB259764_1(AB259764|pid:none) Dictyostelium discoideum macA gene...    88   3e-16
AM180355_3692(AM180355|pid:none) Clostridium difficile 630 compl...    33   7.0
protein update 2009. 3.26
PSORT 

 psg: 0.40  gvh: 0.23  alm: 0.72  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.24  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  8.0 %: cytoplasmic
	  4.0 %: plasma membrane

>> prediction for VSK182 is nuc
5' end seq. ID VSK182F
5' end seq. 
>VSK182F.Seq
GCAACAATCCCATCACCTCAATCTCAACTATTTCCATCAAGTTTATTTTTATCATCACCA
AATTCTGAAGAAACCATTACAATCTTTTTAATGATGTGTGTAAATAGTTTTAAAGCAAAT
AGTAATATATTAGATTTCATTTACACTGTAGAGAAATATCTTCGTCCAAACAAACCAATA
ATTAAAAGTGGTATGTTGGATTTGGATGAATTCAAAGAAAGAGAAGAAGCTTTTGAAAGC
CAATATGTTAAACAAAAAGAAAGAGATGATTTAAAGAGAATGGCTGAAAAGAAGAAACAA
GAAGAAACCAAAAAAGCTGGCTCACAACCAACTCCAAATGCATCATCATCAGCCAACAAT
TCCAAAGCTGCCAAGATTAAGGAAATTGAAGATAAAATTAGCAAATTACAAAAAGAACTT
GCCGATTTAAAAAAATAAATCAAAATCCCTGTGTTTTTATAAATAAATAAATAAATAAAT
ATATTAATATATAAATATATAAATAAAAAATAAAAAAAAAA----------
Length of 5' end seq. 521
3' end seq. ID VSK182Z
3' end seq. 
>VSK182Z.Seq
----------CTGAAAAGAAGAAACAAGAAGAAACCAAAAAAGCTGGCTCACAACCAACT
CCAAATGCATCATCATCAGCCAACAATTCCAAAGCTGCCAAGATTAAGGAAATTGAAGAT
AAAATTAGCAAATTACAAAAAGAACTTGCCGATTTAAAAAAATAAATCAAAATCCCTGTG
TTTTTATAAATAAATAAATAAATAAATATATTAATATATAAATATAAANAAAAAA
Length of 3' end seq. 225
Connected seq. ID VSK182P
Connected seq. 
>VSK182P.Seq
GCAACAATCCCATCACCTCAATCTCAACTATTTCCATCAAGTTTATTTTTATCATCACCA
AATTCTGAAGAAACCATTACAATCTTTTTAATGATGTGTGTAAATAGTTTTAAAGCAAAT
AGTAATATATTAGATTTCATTTACACTGTAGAGAAATATCTTCGTCCAAACAAACCAATA
ATTAAAAGTGGTATGTTGGATTTGGATGAATTCAAAGAAAGAGAAGAAGCTTTTGAAAGC
CAATATGTTAAACAAAAAGAAAGAGATGATTTAAAGAGAATGGCTGAAAAGAAGAAACAA
GAAGAAACCAAAAAAGCTGGCTCACAACCAACTCCAAATGCATCATCATCAGCCAACAAT
TCCAAAGCTGCCAAGATTAAGGAAATTGAAGATAAAATTAGCAAATTACAAAAAGAACTT
GCCGATTTAAAAAAATAAATCAAAATCCCTGTGTTTTTATAAATAAATAAATAAATAAAT
ATATTAATATATAAATATATAAATAAAAAATAAAAAAAAAA----------CTGAAAAGA
AGAAACAAGAAGAAACCAAAAAAGCTGGCTCACAACCAACTCCAAATGCATCATCATCAG
CCAACAATTCCAAAGCTGCCAAGATTAAGGAAATTGAAGATAAAATTAGCAAATTACAAA
AAGAACTTGCCGATTTAAAAAAATAAATCAAAATCCCTGTGTTTTTATAAATAAATAAAT
AAATAAATATATTAATATATAAATATAAANAAAAAA
Length of connected seq. 746
Full length Seq ID -
Full length Seq. -
Length of full length seq. -