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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSK138
Library VS
(Link to library)
Clone ID VSK138
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11694-1
Original site URL
Representative seq. ID VSK138E
(Link to Original site)
Representative DNA sequence 
>VSK138 (VSK138Q) /CSM/VS/VSK1-B/VSK138Q.Seq.d/
ACCGGGTCCCCCCCTCGAGGTCGACCCCGCGTCCGGTTGCTGCTCGTGCTGGTAAGGATT
TAGTATCAAGTCTTGTTTCAGGTTTATTAACAATTGGACCACGTTTTGGTGGTGCCATCG
ATGATAGTGCTCGTGTATTCCAAGATGCAGTCGATAACAATTTACAACCATCACAATTTT
GTTGAAGGTATGAAATCAAAGGGTAAAAGAATTCCAGGTATTGGTCATTTAATTAAATCT
GCCGATGAAATCGATAAAAGAGTAGTATTATTAAAAGATTATGCTTTCACTCATTTCTCA
TCAACAAAATATTTAGAATACGCATTGGAAGTTGAAATATACCCTTCAAAAAGCAAATAA
TTTAATTTTAAATGTTGATGGTTGTATTGGTGTATTATTTTTAGATTTATTACATTCATC
CGGTTTATTCACTCAACATGAAATTAAAGAAATTATAGATGTTGGTTATTTAAATGGTTT
CTTTATTGTTGGTAGAAGTGTTGGTTTAATCGGTCACGCATTAGATCAACGTAGAAATAA
ACAAGGACTTTATAGACACCAAGCTGATGATGTTCATTATGCCCTTTAAAATTTTAATAA
ATATAAATATAAAAATAAAAATAAAATAAAATAAAATAAAACC
sequence update 2001. 3.26
Translated Amino Acid sequence 
pgpplevdpasgccscw*gfsikscfrfinnwttfwwchr**csciprcsr*qfttitil
lkv*nqrvkefqvlvi*lnlpmksike*yy*kimlslishqqni*NTHWKLKYTLQKANN
LILNVDGCIGVLFLDLLHSSGLFTQHEIKEIIDVGYLNGFFIVGRSVGLIGHALDQRRNK
QGLYRHQADDVHYAL*nfnkykyknknkik*nkt
Translated Amino Acid sequence (All Frames) 
Frame A:
tgspprgrprvrlllvlvri*yqvlfqvy*qldhvlvvpsmivlvyskmqsitiynhhnf
vegmkskgkripgighliksadeidkrvvllkdyafthfsstkyleyaleveiypsksk*
fnfkc*wlywciifrfitfirfihst*n*rnyrcwlfkwflycw*kcwfnrsrirst*k*
trtl*tps**cslcplkf**i*i*k*k*nkik*n


Frame B:
pgpplevdpasgccscw*gfsikscfrfinnwttfwwchr**csciprcsr*qfttitil
lkv*nqrvkefqvlvi*lnlpmksike*yy*kimlslishqqni*NTHWKLKYTLQKANN
LILNVDGCIGVLFLDLLHSSGLFTQHEIKEIIDVGYLNGFFIVGRSVGLIGHALDQRRNK
QGLYRHQADDVHYAL*nfnkykyknknkik*nkt


Frame C:
rvppsrstprpvaaragkdlvsslvsglltigprfggaiddsarvfqdavdnnlqpsqfc
*ryeikg*knsrywsfn*icr*nr*kssiikrlcfhsflinkifririgs*nipfkkqii
*f*mlmvvlvyyf*iyyihpvyslnmklkkl*mlvi*mvslllvevlv*svth*invein
kdfidtklmmfimpfkilininikikik*nkik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK138 (VSK138Q) /CSM/VS/VSK1-B/VSK138Q.Seq.d/                       1134   0.0
VHI728 (VHI728Q) /CSM/VH/VHI7-B/VHI728Q.Seq.d/                       1059   0.0
VHG448 (VHG448Q) /CSM/VH/VHG4-B/VHG448Q.Seq.d/                       1059   0.0
VHC389 (VHC389Q) /CSM/VH/VHC3-D/VHC389Q.Seq.d/                       1059   0.0
VHB747 (VHB747Q) /CSM/VH/VHB7-B/VHB747Q.Seq.d/                       1059   0.0
VHA674 (VHA674Q) /CSM/VH/VHA6-D/VHA674Q.Seq.d/                       1059   0.0
VHA368 (VHA368Q) /CSM/VH/VHA3-C/VHA368Q.Seq.d/                       1059   0.0
VFL190 (VFL190Q) /CSM/VF/VFL1-D/VFL190Q.Seq.d/                       1059   0.0
CHL239 (CHL239Q) /CSM/CH/CHL2-B/CHL239Q.Seq.d/                       1059   0.0
CHE602 (CHE602Q) /CSM/CH/CHE6-A/CHE602Q.Seq.d/                       1059   0.0
own update 2003. 1.10
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BE608288|BE608288.1 sq27g07.y1 Gm-c1046 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1046-2101 5' similar to WP:D1005.1 CE06997 ATP CITRATE LYASE ;, mRNA sequence.    56  4e-08  2
BU025332|BU025332.1 QHF8P02.yg.ab1 QH_EFGHJ sunflower RHA280 Helianthus annuus cDNA clone QHF8P02, mRNA sequence.    64  5e-08  2
BU024390|BU024390.1 QHF2h04.yg.ab1 QH_EFGHJ sunflower RHA280 Helianthus annuus cDNA clone QHF2h04, mRNA sequence.    64  6e-08  2
BG652208|BG652208.1 sad75f02.y1 Gm-c1051 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1051-6028 5' similar to TR:Q9XFY7 Q9XFY7 PUTATIVE ATP-DEPENDENT CITRATE LYASE ;, mRNA sequence.    50  2e-06  2
AI960989|AI960989.1 sc93f08.y1 Gm-c1019 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1019-928 5' similar to WP:D1005.1 CE06997 ATP CITRATE LYASE ;, mRNA sequence.    50  2e-06  2
BE020610|BE020610.1 sm51c09.y1 Gm-c1028 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1028-7433 5' similar to SW:ACLY_RAT P16638 ATP-CITRATE ;, mRNA sequence.    50  2e-06  2
AW704694|AW704694.1 sk39d02.y1 Gm-c1028 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1028-4540 5' similar to WP:D1005.1 CE06997 ATP CITRATE LYASE ;, mRNA sequence.    50  3e-06  2
AW317457|AW317457.1 sg50a11.y1 Gm-c1025 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1025-1749 5' similar to WP:D1005.1 CE06997 ATP CITRATE LYASE ;, mRNA sequence.    50  3e-06  2
AV442244|AV442244.1 Arabidopsis thaliana cDNA clone:APZ18g12_r, 5' end.    62  9e-06  1
AY035067|AY035067.1 Arabidopsis thaliana putative ATP citrate lyase (K7J8.14/AT5g49460) mRNA, complete cds.    62  9e-06  1
dna update 2003. 7.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54YA0) RecName: Full=Probable ATP-citrate synthase;          E...   173   2e-88
AJ243483_1(AJ243483|pid:none) Cyanophora paradoxa mRNA for ATP c...   113   1e-49
AK295675_1(AK295675|pid:none) Homo sapiens cDNA FLJ56442 complet...   106   4e-45
(P53396) RecName: Full=ATP-citrate synthase;          EC=2.3.3.8...   106   4e-45
AK304802_1(AK304802|pid:none) Homo sapiens cDNA FLJ55447 complet...   106   4e-45
AK095084_1(AK095084|pid:none) Homo sapiens cDNA FLJ37765 fis, cl...   106   4e-45
AJ851548_1(AJ851548|pid:none) Gallus gallus mRNA for hypothetica...   105   6e-45
(Q32PF2) RecName: Full=ATP-citrate synthase;          EC=2.3.3.8...   105   6e-45
BC080908_1(BC080908|pid:none) Xenopus tropicalis acly protein, m...   107   1e-44
(Q91V92) RecName: Full=ATP-citrate synthase;          EC=2.3.3.8...   105   2e-44
protein update 2009. 3.25
PSORT 

 psg: 0.57  gvh: 0.68  alm: 0.29  top: 0.47  tms: 0.07  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.31  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: Golgi
	 16.0 %: nuclear
	 16.0 %: mitochondrial
	 12.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VSK138 is cyt
5' end seq. ID VSK138F
5' end seq. 
>VSK138F.Seq
ACCGGGTCCCCCCCTCGAGGTCGACCCCGCGTCCGGTTGCTGCTCGTGCTGGTAAGGATT
TAGTATCAAGTCTTGTTTCAGGTTTATTAACAATTGGACCACGTTTTGGTGGTGCCATCG
ATGATAGTGCTCGTGTATTCCAAGATGCAGTCGATAACAATTTACAACCATCACAATTTG
TTGAAGGTATGAAATCAAAGGGTAAAAGAATTCCAGGTATTGGTCATTTAATTAAATCTG
CCGATGAAATCGATAAAAGAGTAGTATTATTAAAAGATTATGCTTTCACTCATTTCTCAT
CAACAAAATATTTAGAATACGCATTGGAAGTTGAAATATACCCTTCAAAAAGCAAATAAT
TTAATTTTAAATGTTGATGGTTGTATTGGTGTATTATTTTTAGATTTATTACATTCATCC
GGTTTATTCACTCAACATGAAATTAAAGAAATTATAGATGTTGGTTATTTAAATGGTTTC
TTTATTGTTGGTAGAAGTGTTGGTTTAATCGGTCACGCATTAGATCAACGTAGAAATAAA
CAAGGACTTTATAGACACCAAGCTGATGATGTTCATTATGCCCTTTAAAATTTTAATAAA
TA----------
Length of 5' end seq. 602
3' end seq. ID VSK138Z
3' end seq. 
>VSK138Z.Seq
----------GGTGGTGCCATCGATGATAGTGCTCGTGTATTCCAAGATGCAGTCGATAA
CAATTTACAACCATCACAATTTTGTTGAAGGTATGAAATCAAAGGGTAAAAGAATTCCAG
GTATTGGTCATTTAATTAAATCTGCCGATGAAATCGATAAAAGAGTAGTATTATTAAAAG
ATTATGCTTTCACTCATTTCTCATCAACAAAATATTTAGAATACGCATTGGAAGTTGAAA
TATACCCTTCAAAAAGCAAATAATTTAATTTTAAATGTTGATGGTTGTATTGGTGTATTA
TTTTTAGATTTATTACATTCATCCGGTTTATTCACTCAACATGAAATTAAAGAAATTATA
GATGTTGGTTATTTAAATGGTTTCTTTATTGTTGGTAGAAGTGTTGGTTTAATCGGTCAC
GCATTAGATCAACGTAGAAATAAACAAGGACTTTATAGACACCAAGCTGATGATGTTCAT
TATGCCCTTTAAAATTTTAATAAATATAAATATAAAAATAAAAATAAAATAAAATAAAAT
AAAACC
Length of 3' end seq. 536
Connected seq. ID VSK138P
Connected seq. 
>VSK138P.Seq
ACCGGGTCCCCCCCTCGAGGTCGACCCCGCGTCCGGTTGCTGCTCGTGCTGGTAAGGATT
TAGTATCAAGTCTTGTTTCAGGTTTATTAACAATTGGACCACGTTTTGGTGGTGCCATCG
ATGATAGTGCTCGTGTATTCCAAGATGCAGTCGATAACAATTTACAACCATCACAATTTG
TTGAAGGTATGAAATCAAAGGGTAAAAGAATTCCAGGTATTGGTCATTTAATTAAATCTG
CCGATGAAATCGATAAAAGAGTAGTATTATTAAAAGATTATGCTTTCACTCATTTCTCAT
CAACAAAATATTTAGAATACGCATTGGAAGTTGAAATATACCCTTCAAAAAGCAAATAAT
TTAATTTTAAATGTTGATGGTTGTATTGGTGTATTATTTTTAGATTTATTACATTCATCC
GGTTTATTCACTCAACATGAAATTAAAGAAATTATAGATGTTGGTTATTTAAATGGTTTC
TTTATTGTTGGTAGAAGTGTTGGTTTAATCGGTCACGCATTAGATCAACGTAGAAATAAA
CAAGGACTTTATAGACACCAAGCTGATGATGTTCATTATGCCCTTTAAAATTTTAATAAA
TA----------GGTGGTGCCATCGATGATAGTGCTCGTGTATTCCAAGATGCAGTCGAT
AACAATTTACAACCATCACAATTTTGTTGAAGGTATGAAATCAAAGGGTAAAAGAATTCC
AGGTATTGGTCATTTAATTAAATCTGCCGATGAAATCGATAAAAGAGTAGTATTATTAAA
AGATTATGCTTTCACTCATTTCTCATCAACAAAATATTTAGAATACGCATTGGAAGTTGA
AATATACCCTTCAAAAAGCAAATAATTTAATTTTAAATGTTGATGGTTGTATTGGTGTAT
TATTTTTAGATTTATTACATTCATCCGGTTTATTCACTCAACATGAAATTAAAGAAATTA
TAGATGTTGGTTATTTAAATGGTTTCTTTATTGTTGGTAGAAGTGTTGGTTTAATCGGTC
ACGCATTAGATCAACGTAGAAATAAACAAGGACTTTATAGACACCAAGCTGATGATGTTC
ATTATGCCCTTTAAAATTTTAATAAATATAAATATAAAAATAAAAATAAAATAAAATAAA
ATAAAACC
Length of connected seq. 1138
Full length Seq ID VSK138E
Full length Seq. 
>VSK138E.Seq
ACCGGGTCCCCCCCTCGAGGTCGACCCCGCGTCCGGTTGCTGCTCGTGCTGGTAAGGATT
TAGTATCAAGTCTTGTTTCAGGTTTATTAACAATTGGACCACGTTTTGGTGGTGCCATCG
ATGATAGTGCTCGTGTATTCCAAGATGCAGTCGATAACAATTTACAACCATCACAATTTT
GTTGAAGGTATGAAATCAAAGGGTAAAAGAATTCCAGGTATTGGTCATTTAATTAAATCT
GCCGATGAAATCGATAAAAGAGTAGTATTATTAAAAGATTATGCTTTCACTCATTTCTCA
TCAACAAAATATTTAGAATACGCATTGGAAGTTGAAATATACCCTTCAAAAAGCAAATAA
TTTAATTTTAAATGTTGATGGTTGTATTGGTGTATTATTTTTAGATTTATTACATTCATC
CGGTTTATTCACTCAACATGAAATTAAAGAAATTATAGATGTTGGTTATTTAAATGGTTT
CTTTATTGTTGGTAGAAGTGTTGGTTTAATCGGTCACGCATTAGATCAACGTAGAAATAA
ACAAGGACTTTATAGACACCAAGCTGATGATGTTCATTATGCCCTTTAAAATTTTAATAA
ATATAAATATAAAAATAAAAATAAAATAAAATAAAATAAAACC
Length of full length seq. 643