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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSK131
Library VS
(Link to library)
Clone ID VSK131
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U06362-1
Original site URL
Representative seq. ID VSK131E
(Link to Original site)
Representative DNA sequence 
>VSK131 (VSK131Q) /CSM/VS/VSK1-B/VSK131Q.Seq.d/
AACAACTGAAAAAAATGAATAAATTTTTAAAAGCATCAACAAGTATTACACGTTTAATAA
ATAGACAACAATCAACTATTGAAACATGCCAATATGCAAAAAGATTTCAAGAGATTGAAG
CAAGTGAATTAAAGAAAGGTATGTGGATTGAACATAAGTCCAAATTATTCCAAATTGAGA
AAGTTGTACATCAAAAGGTTGCAATGAGAGGTGGTTTCATCATTACCGATATGAAGAATG
TTATTGATGGTTCAAAAAGTAATATTAAATTTAGAAGTGCAGAGAATATAGAATTAGTTG
ATCTCTCAAGAAGTACTTATAATTTCGTTGAAACTATCAAAGATGGTAAAGCTTTTGTTT
TTAGAAGAATTGATAGTGAAGGTGATGAAGAAGATGATGAAGATTTTGTTTGTACAAATA
TTTCACAATTAGATGGTTATTCACCATATTTATCTTTATTCGGTGAAGATACTGAATTCC
ATTTTAGAACATATAATGGTAATGTAATTGATTTTAAAGGACCAACACAAATTGATCTTA
CTATTAGATCTGTATCAGATCTCCAAAACAATAATGTTCTTCATTTTGAAAATGGTCGTT
CTTGTAAAGGTCCAATTTATTTAAAACCTGGTGATAAAGTTCAAATTCGTTTACCAGAGG
AAGTTTTCATAACAAAAGTTTAAAAAAA
sequence update 2001. 3.26
Translated Amino Acid sequence 
QLKKMNKFLKASTSITRLINRQQSTIETCQYAKRFQEIEASELKKGMWIEHKSKLFQIEK
VVHQKVAMRGGFIITDMKNVIDGSKSNIKFRSAENIELVDLSRSTYNFVETIKDGKAFVF
RRIDSEGDEEDDEDFVCTNISQLDGYSPYLSLFGEDTEFHFRTYNGNVIDFKGPTQIDLT
IRSVSDLQNNNVLHFENGRSCKGPIYLKPGDKVQIRLPEEVFITKV*k
Translated Amino Acid sequence (All Frames) 
Frame A:
nn*kk*inf*khqqvlhv**idnnqllkhanmqkdfkrlkqvn*rkvcglnispnysklr
klyikrlq*evvsslpi*rmllmvqkvilnlevqri*n*lisqevliislklskmvkllf
leelivkvmkkmmkilfvqifhn*mvihhiylysvkilnsilehimvm*lilkdqhklil
lldlyqisktimffilkmvvlvkvqfi*nlvikfkfvyqrkfs*qkfkk


Frame B:
ttekne*ifksinkyytfnk*ttiny*nmpickkisrd*sk*ikeryvd*t*vqiipn*e
sctskgcnerwfhhyryeecy*wfkk*y*i*kcreyris*slkkyl*fr*nyqrw*sfcf
*kn***r**rr**rfclykyftirwlftififir*ry*ipf*ni*w*cn*f*rtntn*sy
y*icirspkq*cssf*kwsfl*rsnlfktw**ssnsftrgsfhnkslkk


Frame C:
QLKKMNKFLKASTSITRLINRQQSTIETCQYAKRFQEIEASELKKGMWIEHKSKLFQIEK
VVHQKVAMRGGFIITDMKNVIDGSKSNIKFRSAENIELVDLSRSTYNFVETIKDGKAFVF
RRIDSEGDEEDDEDFVCTNISQLDGYSPYLSLFGEDTEFHFRTYNGNVIDFKGPTQIDLT
IRSVSDLQNNNVLHFENGRSCKGPIYLKPGDKVQIRLPEEVFITKV*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK131 (VSK131Q) /CSM/VS/VSK1-B/VSK131Q.Seq.d/                       1320   0.0
VSB387 (VSB387Q) /CSM/VS/VSB3-D/VSB387Q.Seq.d/                        918   0.0
VSH431 (VSH431Q) /CSM/VS/VSH4-B/VSH431Q.Seq.d/                        109   6e-23
SLG783 (SLG783Q) /CSM/SL/SLG7-D/SLG783Q.Seq.d/                         44   0.003
VSG317 (VSG317Q) /CSM/VS/VSG3-A/VSG317Q.Seq.d/                         42   0.011
VSA875 (VSA875Q) /CSM/VS/VSA8-D/VSA875Q.Seq.d/                         42   0.011
VSA687 (VSA687Q) /CSM/VS/VSA6-D/VSA687Q.Seq.d/                         42   0.011
SSC788 (SSC788Q) /CSM/SS/SSC7-D/SSC788Q.Seq.d/                         42   0.011
SLH890 (SLH890Q) /CSM/SL/SLH8-D/SLH890Q.Seq.d/                         42   0.011
FC-AP23 (FC-AP23Q) /CSM/FC/FC-AP/FC-AP23Q.Seq.d/                       42   0.011
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.   898  0.0  2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    34  0.008  10
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38  0.009  13
CB514064|CB514064.1 ssalrgb548123 mixed_tissue Salmo salar cDNA, mRNA sequence.    40  0.040  2
AP006060|AP006060.1 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 2, PAC clone:P0135D07, WORKING DRAFT SEQUENCE, 12 unordered pieces.    40  0.12  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    34  0.13  9
BQ625425|BQ625425.1 rd14f02.y1 Meloidogyne incognita egg SL1 TOPO v1 Meloidogyne incognita cDNA 5' similar to WP:CE16790 B0273.4A locus:unc-5 axon guidance protein ;, mRNA sequence.    48  0.14  1
BM882205|BM882205.1 rb27a07.y1 Meloidogyne incognita egg pAMP1 Bird Meloidogyne incognita cDNA 5' similar to SW:YP68_CAEEL Q09217 HYPOTHETICAL 37.0 KD PROTEIN B0495.8 IN CHROMOSOME II. [1] ;contains element MER31 repetitive element ;, mRNA sequence.    48  0.14  1
AW828823|AW828823.1 ra74e11.y1 Bird-Rao Meloidogyne incognita J2 Meloidogyne incognita cDNA 5' similar to WP:B0273.4A CE16790 ;, mRNA sequence.    48  0.14  1
AC115683|AC115683.2 Dictyostelium discoideum chromosome 2 map complement(6135149-6105997) strain AX4, complete sequence.    34  0.21  3
dna update 2003. 7.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q4FN36) Elongation factor P (EF-P).  &CP000084_578(CP000084|pid...    59   1e-07
(A7HUY3) RecName: Full=Elongation factor P;          Short=EF-P;...    58   3e-07
(Q2NAZ6) Elongation factor P (EF-P).  &CP000157_1053(CP000157|pi...    57   4e-07
(A1UR93) Elongation factor P (EF-P).  &CP000524_157(CP000524|pid...    56   8e-07
AM999887_779(AM999887|pid:none) Wolbachia endosymbiont of Culex ...    55   1e-06
(Q2GG58) Elongation factor P (EF-P).  &CP000236_740(CP000236|pid...    55   1e-06
(Q6FYN9) Elongation factor P (EF-P).  &BX897700_1145(BX897700|pi...    54   3e-06
(A4XJ18) Elongation factor P (EF-P).  &CP000679_1238(CP000679|pi...    54   5e-06
(P0C103) Elongation factor P (EF-P).  &(Q2YRC4) Elongation  &(Q8...    54   5e-06
CP000778_192(CP000778|pid:none) Leptospira biflexa serovar Patoc...    54   5e-06
protein update 2009. 3.25
PSORT 

 psg: 0.74  gvh: 0.29  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 28.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall

>> prediction for VSK131 is mit
5' end seq. ID VSK131F
5' end seq. 
>VSK131F.Seq
AACAACTGAAAAAAATGAATAAATTTTTAAAAGCATCAACAAGTATTACACGTTTAATAA
ATAGACAACAATCAACTATTGAAACATGCCAATATGCAAAAAGATTTCAAGAGATTGAAG
CAAGTGAATTAAAGAAAGGTATGTGGATTGAACATAAGTCCAAATTATTCCAAATTGAGA
AAGTTGTACATCAAAAGGTTGCAATGAGAGGTGGTTTCATCATTACCGATATGAAGAATG
TTATTGATGGTTCAAAAAGTAATATTAAATTTAGAAGTGCAGAGAATATAGAATTAGTTG
ATCTCTCAAGAAGTACTTATAATTTCGTTGAAACTATCAAAGATGGTAAAGCTTTTGTTT
TTAGAAGAATTGATAGTGAAGGTGATGAAGAAGATGATGAAGATTTTGTTTGTACAAATA
TTTCACAATTAGATGGTTATTCACCATATTTATCTTTATTCGGTGAAGATACTGAATTCC
ATTTTAGAACATATAATGGTAATGTAATTGATTTTAAA----------
Length of 5' end seq. 518
3' end seq. ID VSK131Z
3' end seq. 
>VSK131Z.Seq
----------AACAACTGAAAAAAATGAATAAATTTTTAAAAGCATCAACAAGTATTACA
CGTTTAATAAATAGACAACAATCAACTATTGAAACATGCCAATATGCAAAAAGATTTCAA
GAGATTGAAGCAAGTGAATTAAAGAAAGGTATGTGGATTGAACATAAGTCCAAATTATTC
CAAATTGAGAAAGTTGTACATCAAAAGGTTGCAATGAGAGGTGGTTTCATCATTACCGAT
ATGAAGAATGTTATTGATGGTTCAAAAAGTAATATTAAATTTAGAAGTGCAGAGAATATA
GAATTAGTTGATCTCTCAAGAAGTACTTATAATTTCGTTGAAACTATCAAAGATGGTAAA
GCTTTTGTTTTTAGAAGAATTGATAGTGAAGGTGATGAAGAAGATGATGAAGATTTTGTT
TGTACAAATATTTCACAATTAGATGGTTATTCACCATATTTATCTTTATTCGGTGAAGAT
ACTGAATTCCATTTTAGAACATATAATGGTAATGTAATTGATTTTAAAGGACCAACACAA
ATTGATCTTACTATTAGATCTGTATCAGATCTCCAAAACAATAATGTTCTTCATTTTGAA
AATGGTCGTTCTTGTAAAGGTCCAATTTATTTAAAACCTGGTGATAAAGTTCAAATTCGT
TTACCAGAGGAAGTTTTCATAACAAAAGTTTAAAAAAA
Length of 3' end seq. 688
Connected seq. ID VSK131P
Connected seq. 
>VSK131P.Seq
AACAACTGAAAAAAATGAATAAATTTTTAAAAGCATCAACAAGTATTACACGTTTAATAA
ATAGACAACAATCAACTATTGAAACATGCCAATATGCAAAAAGATTTCAAGAGATTGAAG
CAAGTGAATTAAAGAAAGGTATGTGGATTGAACATAAGTCCAAATTATTCCAAATTGAGA
AAGTTGTACATCAAAAGGTTGCAATGAGAGGTGGTTTCATCATTACCGATATGAAGAATG
TTATTGATGGTTCAAAAAGTAATATTAAATTTAGAAGTGCAGAGAATATAGAATTAGTTG
ATCTCTCAAGAAGTACTTATAATTTCGTTGAAACTATCAAAGATGGTAAAGCTTTTGTTT
TTAGAAGAATTGATAGTGAAGGTGATGAAGAAGATGATGAAGATTTTGTTTGTACAAATA
TTTCACAATTAGATGGTTATTCACCATATTTATCTTTATTCGGTGAAGATACTGAATTCC
ATTTTAGAACATATAATGGTAATGTAATTGATTTTAAA----------AACAACTGAAAA
AAATGAATAAATTTTTAAAAGCATCAACAAGTATTACACGTTTAATAAATAGACAACAAT
CAACTATTGAAACATGCCAATATGCAAAAAGATTTCAAGAGATTGAAGCAAGTGAATTAA
AGAAAGGTATGTGGATTGAACATAAGTCCAAATTATTCCAAATTGAGAAAGTTGTACATC
AAAAGGTTGCAATGAGAGGTGGTTTCATCATTACCGATATGAAGAATGTTATTGATGGTT
CAAAAAGTAATATTAAATTTAGAAGTGCAGAGAATATAGAATTAGTTGATCTCTCAAGAA
GTACTTATAATTTCGTTGAAACTATCAAAGATGGTAAAGCTTTTGTTTTTAGAAGAATTG
ATAGTGAAGGTGATGAAGAAGATGATGAAGATTTTGTTTGTACAAATATTTCACAATTAG
ATGGTTATTCACCATATTTATCTTTATTCGGTGAAGATACTGAATTCCATTTTAGAACAT
ATAATGGTAATGTAATTGATTTTAAAGGACCAACACAAATTGATCTTACTATTAGATCTG
TATCAGATCTCCAAAACAATAATGTTCTTCATTTTGAAAATGGTCGTTCTTGTAAAGGTC
CAATTTATTTAAAACCTGGTGATAAAGTTCAAATTCGTTTACCAGAGGAAGTTTTCATAA
CAAAAGTTTAAAAAAA
Length of connected seq. 1206
Full length Seq ID VSK131E
Full length Seq. 
>VSK131E.Seq
AACAACTGAAAAAAATGAATAAATTTTTAAAAGCATCAACAAGTATTACACGTTTAATAA
ATAGACAACAATCAACTATTGAAACATGCCAATATGCAAAAAGATTTCAAGAGATTGAAG
CAAGTGAATTAAAGAAAGGTATGTGGATTGAACATAAGTCCAAATTATTCCAAATTGAGA
AAGTTGTACATCAAAAGGTTGCAATGAGAGGTGGTTTCATCATTACCGATATGAAGAATG
TTATTGATGGTTCAAAAAGTAATATTAAATTTAGAAGTGCAGAGAATATAGAATTAGTTG
ATCTCTCAAGAAGTACTTATAATTTCGTTGAAACTATCAAAGATGGTAAAGCTTTTGTTT
TTAGAAGAATTGATAGTGAAGGTGATGAAGAAGATGATGAAGATTTTGTTTGTACAAATA
TTTCACAATTAGATGGTTATTCACCATATTTATCTTTATTCGGTGAAGATACTGAATTCC
ATTTTAGAACATATAATGGTAATGTAATTGATTTTAAAGGACCAACACAAATTGATCTTA
CTATTAGATCTGTATCAGATCTCCAAAACAATAATGTTCTTCATTTTGAAAATGGTCGTT
CTTGTAAAGGTCCAATTTATTTAAAACCTGGTGATAAAGTTCAAATTCGTTTACCAGAGG
AAGTTTTCATAACAAAAGTTTAAAAAAA
Length of full length seq. 688