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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSJ753
Library VS
(Link to library)
Clone ID VSJ753
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14971-1
Original site URL
Representative seq. ID VSJ753P
(Link to Original site)
Representative DNA sequence 
>VSJ753 (VSJ753Q) /CSM/VS/VSJ7-C/VSJ753Q.Seq.d/
ATAAATTATTATTACATGCATTAAAGAAAGGAAGTACCGATAACATTAGTATTATCGTTG
TTATTTTGTAAGAAAAGAAAACTAAAACCTCAACCATAACATCACACATATCACAACAAA
TCATATCAACAATAAACAACCAAAAACAACACAACAATTCAATAACAAAAGCAACACAAC
TCAATAGGTATAATAGAAAATGTTCTAAAGTACAAAAGAAAAAAAAGAAAAAGAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAANGNAAAAAAAAAXXXXXXXXXXCCANGNGTCCGATAA
ATTATTATTACATGCATTAAAGAAAGGAAGTACCGATAACATTAGTATTATCGTTGTTAT
TTTGTAAGAAAAAGAAAACTAAAACCTCTAACCATAACA
sequence update 2001. 3.22
Translated Amino Acid sequence 
klllhalkkgstdnisiivvil*EKKTKTSTITSHISQQIISTINNQKQHNNSITKATQL
NRYNRKCSKVQKKKKKKKKKKKKKKKKXKKK---

---XXSDKLLLHALKKGSTDNISIIVVIL*eken*nl*p*
Translated Amino Acid sequence (All Frames) 
Frame A:
inyyymh*rkevpitlvlsllfckkrklkpqp*hhtyhnksyqq*ttknnttiq*qkqhn
sigiienvlkykrkkrkrkkkkkkkkkxxkk---

---pxvr*iiitcikerkyr*h*yyrcyfvrkrklkpltit

Frame B:
*iiitcikerkyr*h*yyrcyfvrken*nlnhnithittnhinnkqpkttqqfnnksntt
q*v**kmf*stkekkekekkkkkkkkkxxkk---

---XXSDKLLLHALKKGSTDNISIIVVIL*eken*nl*p*

Frame C:
klllhalkkgstdnisiivvil*EKKTKTSTITSHISQQIISTINNQKQHNNSITKATQL
NRYNRKCSKVQKKKKKKKKKKKKKKKKXKKK---

---xxpinyyymh*rkevpitlvlsllfckkkktktsnhn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ753 (VSJ753Q) /CSM/VS/VSJ7-C/VSJ753Q.Seq.d/                        214   8e-55
VFI716 (VFI716Q) /CSM/VF/VFI7-A/VFI716Q.Seq.d/                        192   3e-48
SLH325 (SLH325Q) /CSM/SL/SLH3-B/SLH325Q.Seq.d/                        192   3e-48
SLE469 (SLE469Q) /CSM/SL/SLE4-C/SLE469Q.Seq.d/                        192   3e-48
SLC729 (SLC729Q) /CSM/SL/SLC7-B/SLC729Q.Seq.d/                        192   3e-48
SLC184 (SLC184Q) /CSM/SL/SLC1-D/SLC184Q.Seq.d/                        192   3e-48
SLC171 (SLC171Q) /CSM/SL/SLC1-C/SLC171Q.Seq.d/                        192   3e-48
SFK807 (SFK807Q) /CSM/SF/SFK8-A/SFK807Q.Seq.d/                        192   3e-48
SFF243 (SFF243Q) /CSM/SF/SFF2-B/SFF243Q.Seq.d/                        192   3e-48
SFE382 (SFE382Q) /CSM/SF/SFE3-D/SFE382Q.Seq.d/                        192   3e-48
own update 2001.11.29
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF121254|AF121254.1 Enterococcus faecium cysteine aminopeptidase (pepC) gene, partial cds; phospho-beta-glucosidase BglB (bglB), beta-glucoside specific transport protein (bglS), transcription antiterminator (bglR), enterocin B precursor (entB), enterocin B immunity protein (eniB), and induction factor-like protein genes, complete cds; and unknown genes.    42  0.004  2
AZ185961|AZ185961.1 SP_1006_A1_F01_T7A Strongylocentrotus purpuratus, purple sea urchin , sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=1006 Col=1 Row=K, DNA sequence.    42  0.21  2
AB062089|AB062089.1 Arabidopsis thaliana DNA, chromosome 1 centromere region, BAC clone:F5E12.    42  0.42  2
AI045771|AI045771.1 UI-R-C1-kl-e-05-0-UI.s1 UI-R-C1 Rattus norvegicus cDNA clone UI-R-C1-kl-e-05-0-UI 3', mRNA sequence.    34  0.42  2
AZ548738|AZ548738.1 ENTGJ32TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  0.87  2
AX344568|AX344568.1 Sequence 19 from Patent WO0200932.            54  1.2  4
BZ513752|BZ513752.1 BOMQY15TF BO_2_3_KB Brassica oleracea genomic clone BOMQY15, DNA sequence.    30  1.2  3
AC009729|AC009729.14 Homo sapiens 12 BAC RP11-1K3 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    44  1.3  1
AC122956|AC122956.4 Rattus norvegicus clone CH230-17E8, WORKING DRAFT SEQUENCE, 1 ordered piece.    44  1.3  1
AX323592|AX323592.1 Sequence 80 from Patent WO0192565.            44  1.3  1
dna update 2003. 7.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000764_1849(CP000764|pid:none) Bacillus cereus subsp. cytotoxi...    32   6.4
DQ374391_1(DQ374391|pid:none) Branchiostoma floridae amiloride-s...    32   8.4
AP009552_5618(AP009552|pid:none) Microcystis aeruginosa NIES-843...    32   8.4
protein update 2009. 3.25
PSORT 

 psg: 0.84  gvh: 0.36  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.45  mip: 0.00
 nuc: 1.34  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: cytoplasmic

>> prediction for VSJ753 is nuc
5' end seq. ID VSJ753F
5' end seq. 
>VSJ753F.Seq
ATAAATTATTATTACATGCATTAAAGAAAGGAAGTACCGATAACATTAGTATTATCGTTG
TTATTTTGTAAGAAAAGAAAACTAAAACCTCAACCATAACATCACACATATCACAACAAA
TCATATCAACAATAAACAACCAAAAACAACACAACAATTCAATAACAAAAGCAACACAAC
TCAATAGGTATAATAGAAAATGTTCTAAAGTACAAAAGAAAAAAAAGAAAAAGAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAANGNAAAAAAAAA----------
Length of 5' end seq. 275
3' end seq. ID VSJ753Z
3' end seq. 
>VSJ753Z.Seq
----------CCANGNGTCCGATAAATTATTATTACATGCATTAAAGAAAGGAAGTACCG
ATAACATTAGTATTATCGTTGTTATTTTGTAAGAAAAAGAAAACTAAAACCTCTAACCAT
AACA
Length of 3' end seq. 114
Connected seq. ID VSJ753P
Connected seq. 
>VSJ753P.Seq
ATAAATTATTATTACATGCATTAAAGAAAGGAAGTACCGATAACATTAGTATTATCGTTG
TTATTTTGTAAGAAAAGAAAACTAAAACCTCAACCATAACATCACACATATCACAACAAA
TCATATCAACAATAAACAACCAAAAACAACACAACAATTCAATAACAAAAGCAACACAAC
TCAATAGGTATAATAGAAAATGTTCTAAAGTACAAAAGAAAAAAAAGAAAAAGAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAANGNAAAAAAAAA----------CCANGNGTCCGATAA
ATTATTATTACATGCATTAAAGAAAGGAAGTACCGATAACATTAGTATTATCGTTGTTAT
TTTGTAAGAAAAAGAAAACTAAAACCTCTAACCATAACA
Length of connected seq. 389
Full length Seq ID -
Full length Seq. -
Length of full length seq. -