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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSJ730
Library VS
(Link to library)
Clone ID VSJ730
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16504-1
Original site URL
Representative seq. ID VSJ730E
(Link to Original site)
Representative DNA sequence 
>VSJ730 (VSJ730Q) /CSM/VS/VSJ7-B/VSJ730Q.Seq.d/
AATTTTTTATAAAAAAAGAATGGAAGAAATAAAAGAAACTGTTGAAAAAAAAAATAAAGA
AAAAAAGTNTTTAAATGATTATAAAATAATTTCTGAAGGTAATAATATTAAAGTTTTACA
AAAAAAAGATTTCAAACCAAAATTTTCATTGGTATCATATAAAAATTATGAACAAAATTT
TGATTTTGAAAATGCTAAAATTGTTTCAACCAATTTTGCCATCGATCGTCATATAAAGAT
GAAGAATCCACCAGATCCATTTGTATTTATTAAAATGCCATCTTCAAAGCTTGTTCAAAG
TCAGTTAAAACTTTACTATGAAAATGAAGGTTATAAGGATATTCCAGTATGGATTACACC
ATGTTCAGCATTTATGAGATACATTAAATCGTATCCAATTGTTGGAACTGTAACAGAGAC
AATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTTATCAACTAGTGAAACCAAAGCATC
AGTTTCAGTCGGATTCTTTGGTTGTGAATCATCGATGGAAGTTACAAGTGGTTATGAATA
TGAAGACACTGTTACATCTGAAGAAACTAGAAGTTGGAGTCAAACTTTAAATGAAGGTTC
TTATATTGTTTATCAAAACGTTTTAGTTTATGCTTATATAATCTATGGTGGTAGTTATAA
ACCATACATTGATCAAATGAATAAATTTAATCCTGGATTAAATATCAAATTCTTTAGAGA
AGATAAATGTATCTTTTTTGTACCAATTAATCGTGATGATGCCTTCACTCTTCGTTATCA
AGATAATACATGGGATCCAGTTGAATATGATGTTTTAATTGATTACTTGGGTCAAAATCA
AGATAAATGGTTTTCTGGTGGTTTACCATGAAAATAATAATAATAATAATAATAATAATT
ATATAAATTACATAAAGGTTTCACTATTGGTGTTTTAATCCGGTATAAATAAAATTCTAA
AAATAAAAAATAAAA
sequence update 2001. 3.26
Translated Amino Acid sequence 
IFYKKRMEEIKETVEKKNKEKKXLNDYKIISEGNNIKVLQKKDFKPKFSLVSYKNYEQNF
DFENAKIVSTNFAIDRHIKMKNPPDPFVFIKMPSSKLVQSQLKLYYENEGYKDIPVWITP
CSAFMRYIKSYPIVGTVTETMEKKKGFTNRFLSTSETKASVSVGFFGCESSMEVTSGYEY
EDTVTSEETRSWSQTLNEGSYIVYQNVLVYAYIIYGGSYKPYIDQMNKFNPGLNIKFFRE
DKCIFFVPINRDDAFTLRYQDNTWDPVEYDVLIDYLGQNQDKWFSGGLP*k********l
yklhkgftigvliryk*nsknkk*
Translated Amino Acid sequence (All Frames) 
Frame A:
nfl*kkngrnkrnc*kkk*rkkvfk*l*nnf*r**y*sftkkrfqtkifigii*kl*tkf
*f*kc*ncfnqfchrssykdeestrsiciy*naifkacsksvktll*k*rl*gyssmdyt
mfsiyeih*ivsncwncnrdngkekrfhq*ilin**nqsisfsrilwl*iidgsykwl*i
*rhcyi*rn*klesnfk*rflyclskrfslclynlww*l*tih*sne*i*swikyqil*r
r*mylfctn*s**clhsslsr*ymgss*i*cfn*llgsksr*mvfwwftmkiiiiiiiii
i*it*rfhywcfnpv*ikf*k*kik


Frame B:
IFYKKRMEEIKETVEKKNKEKKXLNDYKIISEGNNIKVLQKKDFKPKFSLVSYKNYEQNF
DFENAKIVSTNFAIDRHIKMKNPPDPFVFIKMPSSKLVQSQLKLYYENEGYKDIPVWITP
CSAFMRYIKSYPIVGTVTETMEKKKGFTNRFLSTSETKASVSVGFFGCESSMEVTSGYEY
EDTVTSEETRSWSQTLNEGSYIVYQNVLVYAYIIYGGSYKPYIDQMNKFNPGLNIKFFRE
DKCIFFVPINRDDAFTLRYQDNTWDPVEYDVLIDYLGQNQDKWFSGGLP*k********l
yklhkgftigvliryk*nsknkk*


Frame C:
ffikkewkk*kkllkkkikkksx*miik*flkviilkfykkkisnqnfhwyhikimnkil
ilkmlklfqpilpsivi*r*rihqihlyllkchlqslfkvs*nftmkmkvirifqyglhh
vqhl*dtlnriqllel*qrqwkrkkvspidsyqlvkpkhqfqsdslvvnhrwklqvvmnm
ktllhlkklevgvkl*mkvlilfiktf*fmli*smvvvinhtlik*inlild*isnslek
invsflyqlivmmpslfvikiihgiqlnmmf*litwvkikingflvvyhennnnnnnnny
inyikvsllvf*sginkilkiknk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ730 (VSJ730Q) /CSM/VS/VSJ7-B/VSJ730Q.Seq.d/                       1544   0.0
VSD543 (VSD543Q) /CSM/VS/VSD5-B/VSD543Q.Seq.d/                       1544   0.0
VFC716 (VFC716Q) /CSM/VF/VFC7-A/VFC716Q.Seq.d/                       1544   0.0
VFJ763 (VFJ763Q) /CSM/VF/VFJ7-C/VFJ763Q.Seq.d/                       1538   0.0
VFI758 (VFI758Q) /CSM/VF/VFI7-C/VFI758Q.Seq.d/                       1538   0.0
VSI168 (VSI168Q) /CSM/VS/VSI1-C/VSI168Q.Seq.d/                       1536   0.0
VHP648 (VHP648Q) /CSM/VH/VHP6-B/VHP648Q.Seq.d/                       1536   0.0
VSF255 (VSF255Q) /CSM/VS/VSF2-C/VSF255Q.Seq.d/                       1530   0.0
VSF487 (VSF487Q) /CSM/VS/VSF4-D/VSF487Q.Seq.d/                       1524   0.0
VHB403 (VHB403Q) /CSM/VH/VHB4-A/VHB403Q.Seq.d/                       1522   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U48706|U48706.1 Dictyostelium discoideum small aggregate formation (smlA) gene, complete cds.  1298  0.0  5
M19469|M19469.1 Dictyostelium discoideum 109 gene 3, complete cds.   327  e-107  2
X94341|X94341.1 D.discoideum mRNA for unknown protein with homology to spore germination protein.    86  8e-52  6
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          36  0.018  12
AC095627|AC095627.7 Rattus norvegicus clone CH230-8N23, WORKING DRAFT SEQUENCE, 2 unordered pieces.    40  0.29  2
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    34  0.46  12
CA930567|CA930567.1 MTU4CA.P24.B01 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    34  0.76  2
CA930485|CA930485.1 MTU4CA.P23.B04 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    34  0.76  2
CA930468|CA930468.1 MTU4CA.P22.H11 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    34  0.76  2
CA930577|CA930577.1 MTU4CA.P24.B11 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    34  0.76  2
dna update 2003. 7.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116330_42(AC116330|pid:none) Dictyostelium discoideum chromoso...    67   1e-09
AC116330_41(AC116330|pid:none) Dictyostelium discoideum chromoso...    50   1e-04
CP000712_1048(CP000712|pid:none) Pseudomonas putida F1, complete...    42   0.023
DQ790236_1(DQ790236|pid:none) Champereia manillana acetyl co-A c...    37   0.74
DQ790237_1(DQ790237|pid:none) Agonandra macrocarpa acetyl co-A c...    37   1.3
AE001419_5(AE001419|pid:none) Plasmodium falciparum 3D7 chromoso...    37   1.3
AY332625_22(AY332625|pid:none) Campylobacter jejuni serotype HS:...    35   2.8
CP001037_3035(CP001037|pid:none) Nostoc punctiforme PCC 73102, c...    35   4.8
BX545858_22(BX545858|pid:none) Campylobacter jejuni.                   35   4.8
AY934867_3(AY934867|pid:none) Campylobacter jejuni subsp. jejuni...    35   4.8
protein update 2009. 3.25
PSORT 

 psg: 0.30  gvh: 0.11  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.02
 nuc: 0.13  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VSJ730 is nuc
5' end seq. ID VSJ730F
5' end seq. 
>VSJ730F.Seq
AATTTTTTATAAAAAAAGAATGGAAGAAATAAAAGAAACTGTTGAAAAAAAAAATAAAGA
AAAAAAGTNTTTAAATGATTATAAAATAATTTCTGAAGGTAATAATATTAAAGTTTTACA
AAAAAAAGATTTCAAACCAAAATTTTCATTGGTATCATATAAAAATTATGAACAAAATTT
TGATTTTGAAAATGCTAAAATTGTTTCAACCAATTTTGCCATCGATCGTCATATAAAGAT
GAAGAATCCACCAGATCCATTTGTATTTATTAAAATGCCATCTTCAAAGCTTGTTCAAAG
TCAGTTAAAACTTTACTATGAAAATGAAGGTTATAAGGATATTCCAGTATGGATTACACC
ATGTTCAGCATTTATGAGATACATTAAATCGTATCCAATTGTTGGAACTGTAACAGAGAC
AATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTTATCAACTAGNGAAACCAAAGCATC
AGTTTCAGTCGGATTNT----------
Length of 5' end seq. 497
3' end seq. ID VSJ730Z
3' end seq. 
>VSJ730Z.Seq
----------TCAGCATTTATGAGATACATTAAATCGTATCCAATTGTTGGAACTGTAAC
AGAGACAATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTTATCAACTAGTGAAACCAA
AGCATCAGTTTCAGTCGGATTCTTTGGTTGTGAATCATCGATGGAAGTTACAAGTGGTTA
TGAATATGAAGACACTGTTACATCTGAAGAAACTAGAAGTTGGAGTCAAACTTTAAATGA
AGGTTCTTATATTGTTTATCAAAACGTTTTAGTTTATGCTTATATAATCTATGGTGGTAG
TTATAAACCATACATTGATCAAATGAATAAATTTAATCCTGGATTAAATATCAAATTCTT
TAGAGAAGATAAATGTATCTTTTTTGTACCAATTAATCGTGATGATGCCTTCACTCTTCG
TTATCAAGATAATACATGGGATCCAGTTGAATATGATGTTTTAATTGATTACTTGGGTCA
AAATCAAGATAAATGGTTTTCTGGTGGTTTACCATGAAAATAATAATAATAATAATAATA
ATAATTATATAAATTACATAAAGGTTTCACTATTGGTGTTTTAATCCGGTATAAATAAAA
TTCTAAAAATAAAAAATAAAA
Length of 3' end seq. 611
Connected seq. ID VSJ730P
Connected seq. 
>VSJ730P.Seq
AATTTTTTATAAAAAAAGAATGGAAGAAATAAAAGAAACTGTTGAAAAAAAAAATAAAGA
AAAAAAGTNTTTAAATGATTATAAAATAATTTCTGAAGGTAATAATATTAAAGTTTTACA
AAAAAAAGATTTCAAACCAAAATTTTCATTGGTATCATATAAAAATTATGAACAAAATTT
TGATTTTGAAAATGCTAAAATTGTTTCAACCAATTTTGCCATCGATCGTCATATAAAGAT
GAAGAATCCACCAGATCCATTTGTATTTATTAAAATGCCATCTTCAAAGCTTGTTCAAAG
TCAGTTAAAACTTTACTATGAAAATGAAGGTTATAAGGATATTCCAGTATGGATTACACC
ATGTTCAGCATTTATGAGATACATTAAATCGTATCCAATTGTTGGAACTGTAACAGAGAC
AATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTTATCAACTAGNGAAACCAAAGCATC
AGTTTCAGTCGGATTNT----------TCAGCATTTATGAGATACATTAAATCGTATCCA
ATTGTTGGAACTGTAACAGAGACAATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTTA
TCAACTAGTGAAACCAAAGCATCAGTTTCAGTCGGATTCTTTGGTTGTGAATCATCGATG
GAAGTTACAAGTGGTTATGAATATGAAGACACTGTTACATCTGAAGAAACTAGAAGTTGG
AGTCAAACTTTAAATGAAGGTTCTTATATTGTTTATCAAAACGTTTTAGTTTATGCTTAT
ATAATCTATGGTGGTAGTTATAAACCATACATTGATCAAATGAATAAATTTAATCCTGGA
TTAAATATCAAATTCTTTAGAGAAGATAAATGTATCTTTTTTGTACCAATTAATCGTGAT
GATGCCTTCACTCTTCGTTATCAAGATAATACATGGGATCCAGTTGAATATGATGTTTTA
ATTGATTACTTGGGTCAAAATCAAGATAAATGGTTTTCTGGTGGTTTACCATGAAAATAA
TAATAATAATAATAATAATAATTATATAAATTACATAAAGGTTTCACTATTGGTGTTTTA
ATCCGGTATAAATAAAATTCTAAAAATAAAAAATAAAA
Length of connected seq. 1108
Full length Seq ID VSJ730E
Full length Seq. 
>VSJ730E.Seq
AATTTTTTATAAAAAAAGAATGGAAGAAATAAAAGAAACTGTTGAAAAAAAAAATAAAGA
AAAAAAGTNTTTAAATGATTATAAAATAATTTCTGAAGGTAATAATATTAAAGTTTTACA
AAAAAAAGATTTCAAACCAAAATTTTCATTGGTATCATATAAAAATTATGAACAAAATTT
TGATTTTGAAAATGCTAAAATTGTTTCAACCAATTTTGCCATCGATCGTCATATAAAGAT
GAAGAATCCACCAGATCCATTTGTATTTATTAAAATGCCATCTTCAAAGCTTGTTCAAAG
TCAGTTAAAACTTTACTATGAAAATGAAGGTTATAAGGATATTCCAGTATGGATTACACC
ATGTTCAGCATTTATGAGATACATTAAATCGTATCCAATTGTTGGAACTGTAACAGAGAC
AATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTTATCAACTAGTGAAACCAAAGCATC
AGTTTCAGTCGGATTCTTTGGTTGTGAATCATCGATGGAAGTTACAAGTGGTTATGAATA
TGAAGACACTGTTACATCTGAAGAAACTAGAAGTTGGAGTCAAACTTTAAATGAAGGTTC
TTATATTGTTTATCAAAACGTTTTAGTTTATGCTTATATAATCTATGGTGGTAGTTATAA
ACCATACATTGATCAAATGAATAAATTTAATCCTGGATTAAATATCAAATTCTTTAGAGA
AGATAAATGTATCTTTTTTGTACCAATTAATCGTGATGATGCCTTCACTCTTCGTTATCA
AGATAATACATGGGATCCAGTTGAATATGATGTTTTAATTGATTACTTGGGTCAAAATCA
AGATAAATGGTTTTCTGGTGGTTTACCATGAAAATAATAATAATAATAATAATAATAATT
ATATAAATTACATAAAGGTTTCACTATTGGTGTTTTAATCCGGTATAAATAAAATTCTAA
AAATAAAAAATAAAA
Length of full length seq. 975