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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSJ580
Library VS
(Link to library)
Clone ID VSJ580
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10366-1
Original site URL
Representative seq. ID VSJ580P
(Link to Original site)
Representative DNA sequence 
>VSJ580 (VSJ580Q) /CSM/VS/VSJ5-D/VSJ580Q.Seq.d/
AAATACATTTATTACTTTAATCTTTACAATTATTTCAATTTCAATTTGTTTTGGTAGTCA
ATTACCAACAAACTATTATTTCAAAGTTACCCATGTTCCAATTTATTGTATTACCACACC
GTGCAGTCAATACCGTGTCTATAAGGTAAATACTCAAGATCCAGAGATTGTTATTGAAAA
TTTCGTTTTCCCAAATGGAGTTGAACAAAACTATTTCTCACTTCAAGAAGCCAATACTGC
TATCCTTTATGGTACTATCATTGCAAACAGAAATGGTTACAATGTAAATGATTTTAAAGT
AAAAAGAGTTTATAAACAACTTCCACAAGGTAATGCTTTAACATCAACTGATAAATATTA
TATGTTTGCTTCATCAGGTATTGTTTGCATTACAACACCATGCCCATCAGTTTCAGCAAT
TTTATTAAATATTGATAATGCTGCAACCAATGTTAATGATGTTAAGCAACCATATXXXXX
XXXXXATAGCAATTGGTTATTCCAAAAGCAATTTGAGTCAGAATCTAACAGATTGATTGG
TGTTGGNACAATTAATAGTCAAGGTGAAGTGATTGTTTCTTCTTCTTTTGTAAGATTACC
AGATCCAATTTCAAATTGTCCAGCATTACCATTATTAAAATGTCAAGGTGGTCATGTTAC
AGTTTACGAAANAGATTCAAATCGTTGTTTAACAAATCCNCAATGTTCAAATGATAATGA
CACTCGNGCTTGTATCCTTTCAATTCCAACCTGCTCAACTGGTTANGATTTAGNATCTTA
NACTTCTATTTCAACTTGTCCAGCANACAACTGTGATGCTTATTTCTTAACAAAAACTTA
TTGAATTTAATTTAAATACATATATTTATAAATAAATAAAAAAAAATAG
sequence update 2001. 3.22
Translated Amino Acid sequence 
NTFITLIFTIISISICFGSQLPTNYYFKVTHVPIYCITTPCSQYRVYKVNTQDPEIVIEN
FVFPNGVEQNYFSLQEANTAILYGTIIANRNGYNVNDFKVKRVYKQLPQGNALTSTDKYY
MFASSGIVCITTPCPSVSAILLNIDNAATNVNDVKQPY---

---SNWLFQKQFESESNRLIGVGTINSQGEVIVSSSFVRLPDPISNCPALPLLKCQGGHV
TVYEXDSNRCLTNPQCSNDNDTRACILSIPTCSTGXDLXSXTSISTCPAXNCDAYFLTKT
Y*i*fkyiyl*inkkk*
Translated Amino Acid sequence (All Frames) 
Frame A:
kyiyyfnlynyfnfnlfw*sitnkllfqsypcsnllyyhtvqsipcl*gkysrsrdcy*k
frfpkws*tklfltsrsqycyplwyyhckqkwlqck*f*skksl*ttstr*cfnin**il
yvcfiryclhyntmpisfsnfiky**ccnqc**c*ati---

---iaigysksnlsqnltd*lvlxqlivkvk*lfllll*dyqiqfqivqhyhy*nvkvvm
lqftkxiqivv*qixnvqmimtlxlvsfqfqpaqlvxi*xlxllfqlvqxttvmlis*qk
liefnlntyiyk*ikkn

Frame B:
NTFITLIFTIISISICFGSQLPTNYYFKVTHVPIYCITTPCSQYRVYKVNTQDPEIVIEN
FVFPNGVEQNYFSLQEANTAILYGTIIANRNGYNVNDFKVKRVYKQLPQGNALTSTDKYY
MFASSGIVCITTPCPSVSAILLNIDNAATNVNDVKQPY---

---*qlvipkai*vri*qidwcwxn**sr*sdcffffckitrsnfklssitiikmsrwsc
yslrxrfkslfnksxmfk***hsxlypfnsnllnwlxfxilxfyfnlssxql*clflnkn
llnli*ihifink*kki

Frame C:
ihlll*slqlfqfqfvlvvnyqqtiisklpmfqfivlphravntvsir*ilkiqrlllki
sfsqmelnktishfkkpillsfmvlslqtemvtm*milk*kefinnfhkvml*hqlinii
cllhqvlfalqhhahqfqqfy*ilimlqpmlmmlsnh---

---SNWLFQKQFESESNRLIGVGTINSQGEVIVSSSFVRLPDPISNCPALPLLKCQGGHV
TVYEXDSNRCLTNPQCSNDNDTRACILSIPTCSTGXDLXSXTSISTCPAXNCDAYFLTKT
Y*i*fkyiyl*inkkk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ580 (VSJ580Q) /CSM/VS/VSJ5-D/VSJ580Q.Seq.d/                       1631   0.0
VSF679 (VSF679Q) /CSM/VS/VSF6-D/VSF679Q.Seq.d/                        942   0.0
VSI658 (VSI658Q) /CSM/VS/VSI6-C/VSI658Q.Seq.d/                        729   0.0
VSK263 (VSK263Q) /CSM/VS/VSK2-C/VSK263Q.Seq.d/                        724   0.0
VFG311 (VFG311Q) /CSM/VF/VFG3-A/VFG311Q.Seq.d/                        710   0.0
VFN148 (VFN148Q) /CSM/VF/VFN1-B/VFN148Q.Seq.d/                        700   0.0
VSE458 (VSE458Q) /CSM/VS/VSE4-C/VSE458Q.Seq.d/                        694   0.0
VFB209 (VFB209Q) /CSM/VF/VFB2-A/VFB209Q.Seq.d/                        646   0.0
VSI514 (VSI514Q) /CSM/VS/VSI5-A/VSI514Q.Seq.d/                        628   e-179
VFH204 (VFH204Q) /CSM/VF/VFH2-A/VFH204Q.Seq.d/                        488   e-137
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   753  0.0  6
BM399823|BM399823.1 5009-0-62-B02.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    52  0.012  1
AC140660|AC140660.2 Pan troglodytes clone CH251-145M14, WORKING DRAFT SEQUENCE, 7 ordered pieces.    42  0.013  6
AC069127|AC069127.1 Homo sapiens chromosome 8 clone RP11-200A13, WORKING DRAFT SEQUENCE, 15 unordered pieces.    36  0.075  7
AJ010770|AJ010770.1 Homo sapiens hyperion gene, exons 1-50.       44  0.11  5
AC013329|AC013329.3 Homo sapiens chromosome 2 clone RP11-422B1 map 2, WORKING DRAFT SEQUENCE, 8 unordered pieces.    36  0.15  6
AL086049|AL086049.1 Arabidopsis thaliana genome survey sequence T7 end of BAC F11N22 of  IGF library from strain Columbia of Arabidopsis thaliana.    40  0.17  2
AC091047|AC091047.10 Homo sapiens chromosome 8, clone RP11-102F4, complete sequence.    42  0.20  3
AJ517168|AJ517168.1 Arabidopsis thaliana GSS, clone 22B/240.      40  0.28  2
AC004013|AC004013.1 Human BAC clone GS1-541B18 from 7q21, complete sequence.    44  0.28  4
dna update 2003. 7.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116305_112(AC116305|pid:none) Dictyostelium discoideum chromos...   514   e-144
AC116305_115(AC116305|pid:none) Dictyostelium discoideum chromos...   208   2e-52
AC116305_114(AC116305|pid:none) Dictyostelium discoideum chromos...   202   9e-51
AC116921_3(AC116921|pid:none) Dictyostelium discoideum chromosom...    56   2e-06
CP000934_2920(CP000934|pid:none) Cellvibrio japonicus Ueda107, c...    42   0.020
AP009380_919(AP009380|pid:none) Porphyromonas gingivalis ATCC 33...    39   0.22
EF990709_1(EF990709|pid:none) Rotavirus A strain B3458 NSP1 gene...    39   0.29
CT573326_4309(CT573326|pid:none) Pseudomonas entomophila str. L4...    36   1.9
EF560708_1(EF560708|pid:none) Rotavirus A Hu/Dhaka6/BGD/2001/G11...    35   2.4
EU200799_1(EU200799|pid:none) Human rotavirus A isolate N155 NSP...    35   3.2
protein update 2009. 3.25
PSORT 

 psg: 0.98  gvh: 0.65  alm: 0.33  top: 0.57  tms: 0.00  mit: 0.44  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 12.0 %: extracellular, including cell wall
	 12.0 %: nuclear
	 12.0 %: vacuolar
	  8.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: endoplasmic reticulum

>> prediction for VSJ580 is mit
5' end seq. ID VSJ580F
5' end seq. 
>VSJ580F.Seq
AAATACATTTATTACTTTAATCTTTACAATTATTTCAATTTCAATTTGTTTTGGTAGTCA
ATTACCAACAAACTATTATTTCAAAGTTACCCATGTTCCAATTTATTGTATTACCACACC
GTGCAGTCAATACCGTGTCTATAAGGTAAATACTCAAGATCCAGAGATTGTTATTGAAAA
TTTCGTTTTCCCAAATGGAGTTGAACAAAACTATTTCTCACTTCAAGAAGCCAATACTGC
TATCCTTTATGGTACTATCATTGCAAACAGAAATGGTTACAATGTAAATGATTTTAAAGT
AAAAAGAGTTTATAAACAACTTCCACAAGGTAATGCTTTAACATCAACTGATAAATATTA
TATGTTTGCTTCATCAGGTATTGTTTGCATTACAACACCATGCCCATCAGTTTCAGCAAT
TTTATTAAATATTGATAATGCTGCAACCAATGTTAATGATGTTAAGCAACCATAT-----
-----
Length of 5' end seq. 475
3' end seq. ID VSJ580Z
3' end seq. 
>VSJ580Z.Seq
----------ATAGCAATTGGTTATTCCAAAAGCAATTTGAGTCAGAATCTAACAGATTG
ATTGGTGTTGGNACAATTAATAGTCAAGGTGAAGTGATTGTTTCTTCTTCTTTTGTAAGA
TTACCAGATCCAATTTCAAATTGTCCAGCATTACCATTATTAAAATGTCAAGGTGGTCAT
GTTACAGTTTACGAAANAGATTCAAATCGTTGTTTAACAAATCCNCAATGTTCAAATGAT
AATGACACTCGNGCTTGTATCCTTTCAATTCCAACCTGCTCAACTGGTTANGATTTAGNA
TCTTANACTTCTATTTCAACTTGTCCAGCANACAACTGTGATGCTTATTTCTTAACAAAA
ACTTATTGAATTTAATTTAAATACATATATTTATAAATAAATAAAAAAAAATAG
Length of 3' end seq. 404
Connected seq. ID VSJ580P
Connected seq. 
>VSJ580P.Seq
AAATACATTTATTACTTTAATCTTTACAATTATTTCAATTTCAATTTGTTTTGGTAGTCA
ATTACCAACAAACTATTATTTCAAAGTTACCCATGTTCCAATTTATTGTATTACCACACC
GTGCAGTCAATACCGTGTCTATAAGGTAAATACTCAAGATCCAGAGATTGTTATTGAAAA
TTTCGTTTTCCCAAATGGAGTTGAACAAAACTATTTCTCACTTCAAGAAGCCAATACTGC
TATCCTTTATGGTACTATCATTGCAAACAGAAATGGTTACAATGTAAATGATTTTAAAGT
AAAAAGAGTTTATAAACAACTTCCACAAGGTAATGCTTTAACATCAACTGATAAATATTA
TATGTTTGCTTCATCAGGTATTGTTTGCATTACAACACCATGCCCATCAGTTTCAGCAAT
TTTATTAAATATTGATAATGCTGCAACCAATGTTAATGATGTTAAGCAACCATAT-----
-----ATAGCAATTGGTTATTCCAAAAGCAATTTGAGTCAGAATCTAACAGATTGATTGG
TGTTGGNACAATTAATAGTCAAGGTGAAGTGATTGTTTCTTCTTCTTTTGTAAGATTACC
AGATCCAATTTCAAATTGTCCAGCATTACCATTATTAAAATGTCAAGGTGGTCATGTTAC
AGTTTACGAAANAGATTCAAATCGTTGTTTAACAAATCCNCAATGTTCAAATGATAATGA
CACTCGNGCTTGTATCCTTTCAATTCCAACCTGCTCAACTGGTTANGATTTAGNATCTTA
NACTTCTATTTCAACTTGTCCAGCANACAACTGTGATGCTTATTTCTTAACAAAAACTTA
TTGAATTTAATTTAAATACATATATTTATAAATAAATAAAAAAAAATAG
Length of connected seq. 879
Full length Seq ID -
Full length Seq. -
Length of full length seq. -