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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSJ387
Library VS
(Link to library)
Clone ID VSJ387
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14130-1
Original site URL
Representative seq. ID VSJ387P
(Link to Original site)
Representative DNA sequence 
>VSJ387 (VSJ387Q) /CSM/VS/VSJ3-D/VSJ387Q.Seq.d/
GACTTTTTATATTTATTACTATAAATTATATATTTCACAAAAAAGTAACAGTTTGAACAA
ATATGGCTTTAAAGAGAATTAATAAAGAATTATCAGATATTGCTAAAGATCCACCATCCA
GCTGCAGTGCTGGTCCAGATACAGATGATATTTTCCATTGGACAGCTACTATTATGGGTC
CAAAAGACTCACCATACGAAGGAGGTGTTTTCTTTTTAAATATTAATTTCCCAACAGATT
ATCCATTTAAACCACCAAAGATTAGTTTTGTTACCAAAGTTTACCATCCAAATATTAATG
CCAGCGGAAGTATTTGTTTAGATATTTTAAAGGAACAATGGAGTCCAGCCTTAACTATTC
CAAAAGTTTTACTTTCAATTAGTGCCCTTTTATCTGACCCAAATCCAGATGATCCTCTTG
TACCAGATATTGCCAATTTATTTAAAACAGACAAAAATAGATTCGAATCTAATGCCCGGT
GAGTGGACAAGAAAATATGCACAATAATTTTTCACGAATAGATAAGGCAACCCAAAAAAA
AAAXXXXXXXXXXGTCCGGACTTTTTATATTTATTACTATAAATTATATATTTCACAAAA
AAGTAACAGTTTGAACAAATATGGCTTTAAAGAGAATTAATAAAGAATTATCAGATATTG
CTAAAGATCCACCATCCAGCTGCAGTGCTGGTCCANANACAGATGATATTTTCCATTGGA
CAGCTACTATTATGGGTCCAAAAGACTCNCCATACGAAGGAGGTGTTTTCTTTTTAAATA
TTAATTTCCCAACAGATTATCCATTTAAACCNCCAAAGATTAGTTTTGTTACCAAAGTTT
ACCATCCAAATATTAANGCCAGCGGAAGNATTTGTTTAGATATTTTAAAGGAACAATGGA
GNCCAGCCTTAACTATTCCAAAAGTTTTACTTTCAATTAGNGCCCTTTTATCTGACCCAA
ATCCAGATGATCCTCNTGNACCAGATATTGCCNATTTATTTAAAACAGACAAAAATAGAT
TCGAATCTAANGCCCGTGAGTGGACAAGAAAANANGCNCAATAATTTTTCACGAATAGAT
AAGGCAACCCAAAAAAAAAAAATTTTTTTTGGTNAACGACTAAACTTTTAAAATAAACAA
TAGAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
tfyiyyyklyisqksnslnkygfken**riiryc*rstiqlqcwsryr*yfpldsyyygs
krltirrrcflfky*fpnrlsi*ttkd*fcyqslpsky*cqrkylfryfkgtmesslnys
ksftfn*cpfi*pksr*ssctrycqfi*nrqk*iri*CPVSGQENMHNNFSRIDKATQKK
---

---VRTFYIYYYKLYISQKSNSLNKYGFKEN**riiryc*rstiqlqcwsxxr*yfplds
yyygskrlxirrrcflfky*fpnrlsi*txkd*fcyqslpsky*xqrkxlfryfkgtmex
slnysksftfn*xpfi*pksr*ssxtrycxfi*nrqk*iri*xp*vdkkxxxiifhe*ir
qpkkkkfflvnd*tfkinnr
Translated Amino Acid sequence (All Frames) 
Frame A:
dflylll*iiyftkk*qfeqiwl*reliknyqillkihhpaavlvqiqmifsigqlllwv
qkthhtkevfsf*ilisqqiihlnhqrlvllpkftiqilmpaevfv*if*rnngvqp*lf
qkfyfqlvpfyltqiqmillyqilpiylkqtkidsnlmpgewtrkyaq*fftnr*gnpkk
k---

---VRTFYIYYYKLYISQKSNSLNKYGFKEN**riiryc*rstiqlqcwsxxr*yfplds
yyygskrlxirrrcflfky*fpnrlsi*txkd*fcyqslpsky*xqrkxlfryfkgtmex
slnysksftfn*xpfi*pksr*ssxtrycxfi*nrqk*iri*xp*vdkkxxxiifhe*ir
qpkkkkfflvnd*tfkinnr

Frame B:
tfyiyyyklyisqksnslnkygfken**riiryc*rstiqlqcwsryr*yfpldsyyygs
krltirrrcflfky*fpnrlsi*ttkd*fcyqslpsky*cqrkylfryfkgtmesslnys
ksftfn*cpfi*pksr*ssctrycqfi*nrqk*iri*CPVSGQENMHNNFSRIDKATQKK
---

---sglfifitinyifhkkvtv*tnmalkrinkelsdiakdppsscsagpxtddifhwta
timgpkdspyeggvfflninfptdypfkppkisfvtkvyhpnixasgxicldilkeqwxp
altipkvllsixallsdpnpddpxxpdiaxlfktdknrfesxarewtrkxaq*fftnr*g
npkkknffwxttkllk*tie

Frame C:
lfifitinyifhkkvtv*tnmalkrinkelsdiakdppsscsagpdtddifhwtatimgp
kdspyeggvfflninfptdypfkppkisfvtkvyhpninasgsicldilkeqwspaltip
kvllsisallsdpnpddplvpdianlfktdknrfesnar*vdkkictiifhe*irqpkkk
---

---pdflylll*iiyftkk*qfeqiwl*reliknyqillkihhpaavlvxxqmifsigql
llwvqktxhtkevfsf*ilisqqiihlnxqrlvllpkftiqilxpaexfv*if*rnngxq
p*lfqkfyfqlxpfyltqiqmilxxqilpiylkqtkidsnlxpvsgqexxxnnfsridka
tqkkkiffgxrlnf*nkq*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ387 (VSJ387Q) /CSM/VS/VSJ3-D/VSJ387Q.Seq.d/                       1057   0.0
AHN338 (AHN338Q) /CSM/AH/AHN3-B/AHN338Q.Seq.d/                       1041   0.0
VSG818 (VSG818Q) /CSM/VS/VSG8-A/VSG818Q.Seq.d/                       1025   0.0
VSF889 (VSF889Q) /CSM/VS/VSF8-D/VSF889Q.Seq.d/                       1013   0.0
SSF567 (SSF567Q) /CSM/SS/SSF5-C/SSF567Q.Seq.d/                        987   0.0
VSC717 (VSC717Q) /CSM/VS/VSC7-A/VSC717Q.Seq.d/                        870   0.0
SSC652 (SSC652Q) /CSM/SS/SSC6-C/SSC652Q.Seq.d/                        850   0.0
SSJ182 (SSJ182Q) /CSM/SS/SSJ1-D/SSJ182Q.Seq.d/                        587   e-166
SSL483 (SSL483Q) /CSM/SS/SSL4-D/SSL483Q.Seq.d/                        585   e-166
VSJ287 (VSJ287Q) /CSM/VS/VSJ2-D/VSJ287Q.Seq.d/                        543   e-153
own update 2003. 1.10
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AQ842837|AQ842837.1 CpG1269B CpIOWAgDNA1 Cryptosporidium parvum genomic similar to ubiquitin-conjugating enzyme E2-16 KD, DNA sequence.    64  3e-26  6
BI506579|BI506579.1 BB170023A20G10 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170023A20G10 5', mRNA sequence.    54  1e-18  5
BI507129|BI507129.1 BB170024A20H01 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170024A20H01 5', mRNA sequence.    54  2e-18  5
BI505480|BI505480.1 BB170031A10H04 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170031A10H04 5', mRNA sequence.    54  2e-18  5
BI509967|BI509967.1 BB170026A10F07 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170026A10F07 5', mRNA sequence.    54  2e-18  5
BI505398|BI505398.1 BB170020A10G06 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170020A10G06 5', mRNA sequence.    54  1e-15  4
AA695372|AA695372.1 GM02710.5prime GM Drosophila melanogaster ovary BlueScript Drosophila melanogaster cDNA clone GM02710 5prime similar to X62575: eff FBgn0011217 PID:g8783 SWISS-PROT:P25867, mRNA sequence.    52  3e-14  3
AI061813|AI061813.1 LD34950.5prime LD Drosophila melanogaster embryo pOT2 Drosophila melanogaster cDNA clone LD34950 5prime similar to X62575: eff FBgn0011217 PID:g8783 SWISS-PROT:P25867, mRNA sequence.    52  6e-14  3
AA263956|AA263956.1 LD06596.5prime LD Drosophila melanogaster embryo BlueScript Drosophila melanogaster cDNA clone LD06596 5prime, mRNA sequence.    52  6e-14  3
AI512403|AI512403.1 LD44271.5prime LD Drosophila melanogaster embryo pOT2 Drosophila melanogaster cDNA clone LD44271 5prime similar to X62575: eff FBgn0011217 PID:g8783 SWISS-PROT:P25867, mRNA sequence.    52  6e-14  3
dna update 2003. 7.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P46595) RecName: Full=Ubiquitin-conjugating enzyme E2 4;       ...   237   5e-61
AP007167_66(AP007167|pid:none) Aspergillus oryzae RIB40 genomic ...   235   2e-60
AM920435_793(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   235   2e-60
AE017346_284(AE017346|pid:none) Cryptococcus neoformans var. neo...   234   4e-60
CR382130_616(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   233   9e-60
AK009276_1(AK009276|pid:none) Mus musculus adult male tongue cDN...   233   1e-59
AK095822_1(AK095822|pid:none) Homo sapiens cDNA FLJ38503 fis, cl...   232   2e-59
EU244369_1(EU244369|pid:none) Haliotis diversicolor clone HDr4CJ...   232   2e-59
(P61077) RecName: Full=Ubiquitin-conjugating enzyme E2 D3;      ...   231   3e-59
(O74196) RecName: Full=Ubiquitin-conjugating enzyme E2-16 kDa;  ...   230   6e-59
protein update 2009. 3.25
PSORT 

 psg: 0.75  gvh: 0.24  alm: 0.64  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial

>> prediction for VSJ387 is cyt
5' end seq. ID VSJ387F
5' end seq. 
>VSJ387F.Seq
GACTTTTTATATTTATTACTATAAATTATATATTTCACAAAAAAGTAACAGTTTGAACAA
ATATGGCTTTAAAGAGAATTAATAAAGAATTATCAGATATTGCTAAAGATCCACCATCCA
GCTGCAGTGCTGGTCCAGATACAGATGATATTTTCCATTGGACAGCTACTATTATGGGTC
CAAAAGACTCACCATACGAAGGAGGTGTTTTCTTTTTAAATATTAATTTCCCAACAGATT
ATCCATTTAAACCACCAAAGATTAGTTTTGTTACCAAAGTTTACCATCCAAATATTAATG
CCAGCGGAAGTATTTGTTTAGATATTTTAAAGGAACAATGGAGTCCAGCCTTAACTATTC
CAAAAGTTTTACTTTCAATTAGTGCCCTTTTATCTGACCCAAATCCAGATGATCCTCTTG
TACCAGATATTGCCAATTTATTTAAAACAGACAAAAATAGATTCGAATCTAATGCCCGGT
GAGTGGACAAGAAAATATGCACAATAATTTTTCACGAATAGATAAGGCAACCCAAAAAAA
AAA----------
Length of 5' end seq. 543
3' end seq. ID VSJ387Z
3' end seq. 
>VSJ387Z.Seq
----------GTCCGGACTTTTTATATTTATTACTATAAATTATATATTTCACAAAAAAG
TAACAGTTTGAACAAATATGGCTTTAAAGAGAATTAATAAAGAATTATCAGATATTGCTA
AAGATCCACCATCCAGCTGCAGTGCTGGTCCANANACAGATGATATTTTCCATTGGACAG
CTACTATTATGGGTCCAAAAGACTCNCCATACGAAGGAGGTGTTTTCTTTTTAAATATTA
ATTTCCCAACAGATTATCCATTTAAACCNCCAAAGATTAGTTTTGTTACCAAAGTTTACC
ATCCAAATATTAANGCCAGCGGAAGNATTTGTTTAGATATTTTAAAGGAACAATGGAGNC
CAGCCTTAACTATTCCAAAAGTTTTACTTTCAATTAGNGCCCTTTTATCTGACCCAAATC
CAGATGATCCTCNTGNACCAGATATTGCCNATTTATTTAAAACAGACAAAAATAGATTCG
AATCTAANGCCCGTGAGTGGACAAGAAAANANGCNCAATAATTTTTCACGAATAGATAAG
GCAACCCAAAAAAAAAAAATTTTTTTTGGTNAACGACTAAACTTTTAAAATAAACAATAG
AA
Length of 3' end seq. 592
Connected seq. ID VSJ387P
Connected seq. 
>VSJ387P.Seq
GACTTTTTATATTTATTACTATAAATTATATATTTCACAAAAAAGTAACAGTTTGAACAA
ATATGGCTTTAAAGAGAATTAATAAAGAATTATCAGATATTGCTAAAGATCCACCATCCA
GCTGCAGTGCTGGTCCAGATACAGATGATATTTTCCATTGGACAGCTACTATTATGGGTC
CAAAAGACTCACCATACGAAGGAGGTGTTTTCTTTTTAAATATTAATTTCCCAACAGATT
ATCCATTTAAACCACCAAAGATTAGTTTTGTTACCAAAGTTTACCATCCAAATATTAATG
CCAGCGGAAGTATTTGTTTAGATATTTTAAAGGAACAATGGAGTCCAGCCTTAACTATTC
CAAAAGTTTTACTTTCAATTAGTGCCCTTTTATCTGACCCAAATCCAGATGATCCTCTTG
TACCAGATATTGCCAATTTATTTAAAACAGACAAAAATAGATTCGAATCTAATGCCCGGT
GAGTGGACAAGAAAATATGCACAATAATTTTTCACGAATAGATAAGGCAACCCAAAAAAA
AAA----------GTCCGGACTTTTTATATTTATTACTATAAATTATATATTTCACAAAA
AAGTAACAGTTTGAACAAATATGGCTTTAAAGAGAATTAATAAAGAATTATCAGATATTG
CTAAAGATCCACCATCCAGCTGCAGTGCTGGTCCANANACAGATGATATTTTCCATTGGA
CAGCTACTATTATGGGTCCAAAAGACTCNCCATACGAAGGAGGTGTTTTCTTTTTAAATA
TTAATTTCCCAACAGATTATCCATTTAAACCNCCAAAGATTAGTTTTGTTACCAAAGTTT
ACCATCCAAATATTAANGCCAGCGGAAGNATTTGTTTAGATATTTTAAAGGAACAATGGA
GNCCAGCCTTAACTATTCCAAAAGTTTTACTTTCAATTAGNGCCCTTTTATCTGACCCAA
ATCCAGATGATCCTCNTGNACCAGATATTGCCNATTTATTTAAAACAGACAAAAATAGAT
TCGAATCTAANGCCCGTGAGTGGACAAGAAAANANGCNCAATAATTTTTCACGAATAGAT
AAGGCAACCCAAAAAAAAAAAATTTTTTTTGGTNAACGACTAAACTTTTAAAATAAACAA
TAGAA
Length of connected seq. 1135
Full length Seq ID -
Full length Seq. -
Length of full length seq. -