>VSJ323 (VSJ323Q) /CSM/VS/VSJ3-A/VSJ323Q.Seq.d/
CTGGGTACCCGGGCCCCCCCTCGNGGTCGACCCACGCCGTCCGCAAGATATTTTACACAA
ATAAAATATTTAAACATATTAAAACAAACAAATAAATAAAATAAAATAATTAATTAAATA
TATATATATCAGTTTTTTTTTTTCATTACAATATATTAAAAAAAAAAXXXXXXXXXXGTT
TACAATTNCCACCACCACCAAAGACTATTGCTGATGGTGTTAAAAGAGATTACGTCTTAG
TTGGTGGTTTGGCTGCCACTATTGTTGCCTTAACAGCCGGTATTTATAACCATTCAAAAG
GTGGTAGTCAACTCACTACAAATCGTTTATTAAAAGGAAGAATTTGGGCTCAAGCATTCA
CCGTTGTTGCTATGGGTTGGTATGCATTCGCTTATCAAAGAGATTTGGATGAAGCTGAAG
CATCCAAACATAGAAAGGTTATCTTAACATAAACTTTTTAGAAAATGGATATCTTTACGA
AAATATATAAATACTTTTTCTTTATATTTTTCCTTTTAATAACATATGCAATCATTTATT
CTTTAATCTCCTCTCGTTCTTGAAAATCCTTTTTTGTTATAATTCCTCTTCCTTCCACCT
TCCCTTCTTATAATACTTCCTTCCACCTTCCCTTCCCTTAAAAAAATAATGGTATGGTGN
AAANAAAACAACCGCCATTAANAAAATAA

wvpgpplxvdprrpqdilhk*ni*ty*nkqinkik*LIKYIYISFFFSLQYIKKK---

---LQXPPPPKTIADGVKRDYVLVGGLAATIVALTAGIYNHSKGGSQLTTNRLLKGRIWA
QAFTVVAMGWYAFAYQRDLDEAEASKHRKVILT*tf*kmdiftkiykyfffiffllitya
iiyslissrs*ksffviiplpstfpsyntsfhlpfp*knngmvxxkqpplxk*

Frame A:
lgtrapprgrptpsaryftqikylnilkqtnk*nkiin*iyiyqffffitiy*kk---

---vynxhhhqrlllmvlkeits*lvvwlplllp*qpvfitiqkvvvnslqivy*keefg
lkhsplllwvgmhslikeiwmklkhpnierls*hklfrkwislrkyintfslyfsf**hm
qsfil*splvlenpfll*flflppslliilpstfpslkkimvwxkxnnrh*xn

Frame B:
wvpgpplxvdprrpqdilhk*ni*ty*nkqinkik*LIKYIYISFFFSLQYIKKK---

---ftixtttkdyc*wc*krlrlswwfgchycclnsryl*pfkrw*sthyksfikrknlg
ssihrccyglvcirlskrfg*s*siqt*kgylninflengylyeni*ilflyifpfnnic
nhlffnllsflkilfcynsssfhlpfl*yflppslplkk*wygxxkttaixki

Frame C:
gypgppsxsthavrkifytnkifkhiktnk*ik*nn*lniyisvfffhynilkkk---

---LQXPPPPKTIADGVKRDYVLVGGLAATIVALTAGIYNHSKGGSQLTTNRLLKGRIWA
QAFTVVAMGWYAFAYQRDLDEAEASKHRKVILT*tf*kmdiftkiykyfffiffllitya
iiyslissrs*ksffviiplpstfpsyntsfhlpfp*knngmvxxkqpplxk*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ323 (VSJ323Q) /CSM/VS/VSJ3-A/VSJ323Q.Seq.d/                        543   e-153
VSK172 (VSK172Q) /CSM/VS/VSK1-C/VSK172Q.Seq.d/                        533   e-150
VSI555 (VSI555Q) /CSM/VS/VSI5-C/VSI555Q.Seq.d/                        529   e-149
VSB336 (VSB336Q) /CSM/VS/VSB3-B/VSB336Q.Seq.d/                        523   e-147
VSF346 (VSF346Q) /CSM/VS/VSF3-B/VSF346Q.Seq.d/                        519   e-146
VSF834 (VSF834Q) /CSM/VS/VSF8-B/VSF834Q.Seq.d/                        515   e-145
VSC657 (VSC657Q) /CSM/VS/VSC6-C/VSC657Q.Seq.d/                        494   e-138
VSJ865 (VSJ865Q) /CSM/VS/VSJ8-C/VSJ865Q.Seq.d/                        260   3e-68
VSE415 (VSE415Q) /CSM/VS/VSE4-A/VSE415Q.Seq.d/                        260   3e-68
VSA740 (VSA740Q) /CSM/VS/VSA7-B/VSA740Q.Seq.d/                        216   3e-55

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BQ430719|BQ430719.1 AGENCOURT_7766516 NIH_MGC_68 Homo sapiens cDNA clone IMAGE:6022053 5', mRNA sequence.    42  0.22  2
AQ299266|AQ299266.1 HS_2223_B2_F02_MR CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2223 Col=4 Row=L, DNA sequence.    44  2.2  1
BI422362|BI422362.1 EST533028 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC70C24 5' end, mRNA sequence.    42  8.8  1
AC111606|AC111606.4 Rattus norvegicus clone CH230-199L6, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    42  8.8  1
AQ769833|AQ769833.1 HS_3170_B2_F10_T7C CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3170 Col=20 Row=L, genomic survey sequence.    42  8.8  1
AL806521|AL806521.9 Mouse DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP23-385N7.    42  8.8  1
BG780646|BG780646.1 SEAUMC000591 Sea urchin primary mesenchyme cell cDNA library Strongylocentrotus purpuratus cDNA clone 91222952_E16_054_PC_0024_B1_MR_C08 5', mRNA sequence.    42  8.8  1
AQ395691|AQ395691.1 CITBI-E1-2552I9.TR CITBI-E1 Homo sapiens genomic clone 2552I9, genomic survey sequence.    42  8.8  1
AW929334|AW929334.1 EST338122 tomato flower buds 8 mm to pre-anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOC7G22 5', mRNA sequence.    42  8.8  1
AC083869|AC083869.2 Homo sapiens chromosome 7 clone RP11-733N14, complete sequence.    42     43  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM920428_774(AM920428|pid:none) Penicillium chrysogenum Wisconsi...    38   0.31
AL591688_2650(AL591688|pid:none) Sinorhizobium meliloti 1021 com...    35   1.5
(Q9BW72) RecName: Full=HIG1 domain family member 2A;  &AX888103_...    35   2.0
AP007157_670(AP007157|pid:none) Aspergillus oryzae RIB40 genomic...    35   2.6
EF710650_10(EF710650|pid:none) Glyptapanteles flavicoxis clone B...    33   5.8
EF710656_15(EF710656|pid:none) Glyptapanteles indiensis clone BA...    33   5.8
AL627362_63(AL627362|pid:none) DNA centromeric region sequence f...    33   7.6
AE005673_727(AE005673|pid:none) Caulobacter crescentus CB15, com...    33   9.9

 psg: 0.94  gvh: 0.56  alm: 0.26  top: 0.70  tms: 0.07  mit: 0.32  mip: 0.00
 nuc: 0.02  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.07  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VSJ323 is cyt

>VSJ323F.Seq
CTGGGTACCCGGGCCCCCCCTCGNGGTCGACCCACGCCGTCCGCAAGATATTTTACACAA
ATAAAATATTTAAACATATTAAAACAAACAAATAAATAAAATAAAATAATTAATTAAATA
TATATATATCAGTTTTTTTTTTTCATTACAATATATTAAAAAAAAAA----------

>VSJ323Z.Seq
----------GTTTACAATTNCCACCACCACCAAAGACTATTGCTGATGGTGTTAAAAGA
GATTACGTCTTAGTTGGTGGTTTGGCTGCCACTATTGTTGCCTTAACAGCCGGTATTTAT
AACCATTCAAAAGGTGGTAGTCAACTCACTACAAATCGTTTATTAAAAGGAAGAATTTGG
GCTCAAGCATTCACCGTTGTTGCTATGGGTTGGTATGCATTCGCTTATCAAAGAGATTTG
GATGAAGCTGAAGCATCCAAACATAGAAAGGTTATCTTAACATAAACTTTTTAGAAAATG
GATATCTTTACGAAAATATATAAATACTTTTTCTTTATATTTTTCCTTTTAATAACATAT
GCAATCATTTATTCTTTAATCTCCTCTCGTTCTTGAAAATCCTTTTTTGTTATAATTCCT
CTTCCTTCCACCTTCCCTTCTTATAATACTTCCTTCCACCTTCCCTTCCCTTAAAAAAAT
AATGGTATGGTGNAAANAAAACAACCGCCATTAANAAAATAA

>VSJ323P.Seq
CTGGGTACCCGGGCCCCCCCTCGNGGTCGACCCACGCCGTCCGCAAGATATTTTACACAA
ATAAAATATTTAAACATATTAAAACAAACAAATAAATAAAATAAAATAATTAATTAAATA
TATATATATCAGTTTTTTTTTTTCATTACAATATATTAAAAAAAAAA----------GTT
TACAATTNCCACCACCACCAAAGACTATTGCTGATGGTGTTAAAAGAGATTACGTCTTAG
TTGGTGGTTTGGCTGCCACTATTGTTGCCTTAACAGCCGGTATTTATAACCATTCAAAAG
GTGGTAGTCAACTCACTACAAATCGTTTATTAAAAGGAAGAATTTGGGCTCAAGCATTCA
CCGTTGTTGCTATGGGTTGGTATGCATTCGCTTATCAAAGAGATTTGGATGAAGCTGAAG
CATCCAAACATAGAAAGGTTATCTTAACATAAACTTTTTAGAAAATGGATATCTTTACGA
AAATATATAAATACTTTTTCTTTATATTTTTCCTTTTAATAACATATGCAATCATTTATT
CTTTAATCTCCTCTCGTTCTTGAAAATCCTTTTTTGTTATAATTCCTCTTCCTTCCACCT
TCCCTTCTTATAATACTTCCTTCCACCTTCCCTTCCCTTAAAAAAATAATGGTATGGTGN
AAANAAAACAACCGCCATTAANAAAATAA