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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSJ304
Library VS
(Link to library)
Clone ID VSJ304
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U02098-1
Original site URL
Representative seq. ID VSJ304P
(Link to Original site)
Representative DNA sequence 
>VSJ304 (VSJ304Q) /CSM/VS/VSJ3-A/VSJ304Q.Seq.d/
AAAAAACTAAACTAAATATTTGAAATGTCAACATCATCAGTCCCAAGTTTAACTTATTTC
CAAGGTCGTGGATTAGGTCAATTCTCTCGTGTTTTATTAAGTTATCTTGGAATTCCATAT
GAAAATATTACAGTTACTGAAATTTCAGATGCCCTTCGTGCAACTCTTCCATATGGTCAA
TTACCAATTTATCGTGATGGTGATTTTGTTCTTACACAATCATCTACCATTGCAAGATAC
ATTGCCAAAAAACACAACTTTATGGGTAAAAATCTTGAAGAAGAATTTTTAGTTGATCXX
XXXXXXXXCCACGCGTCCCGAAAAAACTAAANCTAAATATTTGAAATGTCAACATCATCA
GTCCCAAGTTTAACTTATTTCCAAGGTCGTGGATTAGGTCAATTCTCTCGTGTTTTATTA
AGTTATCTTGGAATTCCATATGAAAATATTACAGTTACTGAAATTTCAGATGCCCTTCGT
GCAACTCTTCCATATGGTCAATTACCAATTTATCGTGATGGTGATTTTGTTCTTACACAA
TCATCTACCATTGCAAGATACATTGCCAAAAAACACAACTTTATGGGTAAAAATCTTGAA
GAAGAATTTTTAGTTGATCAAATTGTTACTGCAATTCACGCTGATATTTTCCCAGCTTTT
AATAATCCAGTTCCAGAAAAACTCCAAAAACTTTATGAAAAATACTTTGGTTCATTTGAA
AAGAAATTACAAGAAACTGGATTCTTAGTTGGATCATCAGTTACATTAGCTGATTTATAT
GTTTATGTTGGTTTTGATTACATTAGATTCAGAGGTGAAGCAGCCTTATCCAGTGAATTA
TCAGATGAAAAGTATCCAAAAATCGCTGAATTAAAGAAATTTTTTGAATCAAATGAAGGT
GTAGCAAAATATATTAAAGAAAGACCAGAAACTAAATTTTAAACTGTTTTCATCACCATT
sequence update 2001. 3.22
Translated Amino Acid sequence 
kkln*IFEMSTSSVPSLTYFQGRGLGQFSRVLLSYLGIPYENITVTEISDALRATLPYGQ
LPIYRDGDFVLTQSSTIARYIAKKHNFMGKNLEEEFLVD---

---TRPEKTKXKYLKCQHHQSQV*liskvvd*vnslvfy*vilefhmkilqllkfqmpfv
qlfhmvnyqfivmvilflhnhlplqdtlpknttlwvkilkknf*liklllqftlifsqll
iiqfqknsknfmkntlvhlkrnykklds*ldhqlh*liymfmlvlitldsevkqpypvny
qmksiqksln*rnflnqmkv*qnilkkdqklnfklfssp
Translated Amino Acid sequence (All Frames) 
Frame A:
kkln*IFEMSTSSVPSLTYFQGRGLGQFSRVLLSYLGIPYENITVTEISDALRATLPYGQ
LPIYRDGDFVLTQSSTIARYIAKKHNFMGKNLEEEFLVD---

---prvpkklxlni*nvniispkfnlfprswirsilscfiklswnsi*kyysy*nfrcps
cnssiwsitnls*w*fcsytiiyhckihcqktqlyg*ks*rrifs*sncycnsr*yfpsf
**sssrktpktl*kilwfi*keitrnwilswiisyis*ficlcwf*lh*iqr*ssliq*i
ir*kvsknr*ikeif*ik*rcskiy*rktrn*ilncfhhh

Frame B:
kn*tkylkcqhhqsqv*liskvvd*vnslvfy*vilefhmkilqllkfqmpfvqlfhmvn
yqfivmvilflhnhlplqdtlpknttlwvkilkknf*li---

---hasrkn*x*ifemstssvpsltyfqgrglgqfsrvllsylgipyenitvteisdalr
atlpygqlpiyrdgdfvltqsstiaryiakkhnfmgknleeeflvdqivtaihadifpaf
nnpvpeklqklyekyfgsfekklqetgflvgssvtladlyvyvgfdyirfrgeaalssel
sdekypkiaelkkffesnegvakyikerpetkf*tvfiti

Frame C:
ktklni*nvniispkfnlfprswirsilscfiklswnsi*kyysy*nfrcpscnssiwsi
tnls*w*fcsytiiyhckihcqktqlyg*ks*rrifs*---

---TRPEKTKXKYLKCQHHQSQV*liskvvd*vnslvfy*vilefhmkilqllkfqmpfv
qlfhmvnyqfivmvilflhnhlplqdtlpknttlwvkilkknf*liklllqftlifsqll
iiqfqknsknfmkntlvhlkrnykklds*ldhqlh*liymfmlvlitldsevkqpypvny
qmksiqksln*rnflnqmkv*qnilkkdqklnfklfssp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ304 (VSJ304Q) /CSM/VS/VSJ3-A/VSJ304Q.Seq.d/                       1838   0.0
SSG319 (SSG319Q) /CSM/SS/SSG3-A/SSG319Q.Seq.d/                       1247   0.0
VSB769 (VSB769Q) /CSM/VS/VSB7-C/VSB769Q.Seq.d/                       1241   0.0
VSD725 (VSD725Q) /CSM/VS/VSD7-B/VSD725Q.Seq.d/                       1235   0.0
VSD893 (VSD893Q) /CSM/VS/VSD8-D/VSD893Q.Seq.d/                       1231   0.0
VSJ861 (VSJ861Q) /CSM/VS/VSJ8-C/VSJ861Q.Seq.d/                       1229   0.0
VSI418 (VSI418Q) /CSM/VS/VSI4-A/VSI418Q.Seq.d/                       1221   0.0
VSI291 (VSI291Q) /CSM/VS/VSI2-D/VSI291Q.Seq.d/                       1221   0.0
VSI568 (VSI568Q) /CSM/VS/VSI5-C/VSI568Q.Seq.d/                       1199   0.0
VSD210 (VSD210Q) /CSM/VS/VSD2-A/VSD210Q.Seq.d/                       1178   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   569  0.0  11
Z54216|Z54216.1 Caenorhabditis elegans cosmid T24H10.             36  0.091  5
AQ081636|AQ081636.1 RPCI11-54N17.TJ RPCI-11 Homo sapiens genomic clone RPCI-11-54N17, genomic survey sequence.    48  0.20  1
AQ082813|AQ082813.1 RPCI11-54N18.TJ RPCI-11 Homo sapiens genomic clone RPCI-11-54N18, genomic survey sequence.    48  0.20  1
AC006651|AC006651.2 Caenorhabditis elegans cosmid H06I04, complete sequence.    42  0.35  3
AX344568|AX344568.1 Sequence 19 from Patent WO0200932.            34  0.51  7
AB011476|AB011476.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MDA7.    40  0.80  4
AC006791|AC006791.1 Caenorhabditis elegans clone Y4C6A, complete sequence.    38  1.1  4
AC106150|AC106150.4 Rattus norvegicus clone CH230-18J14, WORKING DRAFT SEQUENCE, 2 unordered pieces.    44  1.4  3
AC142096|AC142096.9 Medicago truncatula clone mth2-6m16, WORKING DRAFT SEQUENCE, 9 unordered pieces.    40  1.8  3
dna update 2003. 7.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q556G3) RecName: Full=Putative glutathione S-transferase alpha-...   393   e-108
(Q54VI4) RecName: Full=Putative glutathione S-transferase alpha-...   147   4e-34
(Q55FF3) RecName: Full=Putative glutathione S-transferase alpha-...   142   2e-32
(Q86AU1) RecName: Full=Putative glutathione S-transferase alpha-...   137   7e-31
(Q54QV7) RecName: Full=Putative glutathione S-transferase alpha-...   113   1e-23
T21898(T21898) hypothetical protein F37B1.3 - Caenorhabditis ele...    93   1e-17
BC077016_1(BC077016|pid:none) Xenopus tropicalis MGC89746 protei...    90   1e-16
BC060462_1(BC060462|pid:none) Xenopus laevis hypothetical protei...    86   2e-15
AF525020_1(AF525020|pid:none) Xenopus laevis glutathione s-trans...    84   9e-15
BC053774_1(BC053774|pid:none) Xenopus laevis glutathione s-trans...    84   9e-15
protein update 2009. 3.25
PSORT 

 psg: 0.75  gvh: 0.39  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for VSJ304 is cyt
5' end seq. ID VSJ304F
5' end seq. 
>VSJ304F.Seq
AAAAAACTAAACTAAATATTTGAAATGTCAACATCATCAGTCCCAAGTTTAACTTATTTC
CAAGGTCGTGGATTAGGTCAATTCTCTCGTGTTTTATTAAGTTATCTTGGAATTCCATAT
GAAAATATTACAGTTACTGAAATTTCAGATGCCCTTCGTGCAACTCTTCCATATGGTCAA
TTACCAATTTATCGTGATGGTGATTTTGTTCTTACACAATCATCTACCATTGCAAGATAC
ATTGCCAAAAAACACAACTTTATGGGTAAAAATCTTGAAGAAGAATTTTTAGTTGATC--
--------
Length of 5' end seq. 298
3' end seq. ID VSJ304Z
3' end seq. 
>VSJ304Z.Seq
----------CCACGCGTCCCGAAAAAACTAAANCTAAATATTTGAAATGTCAACATCAT
CAGTCCCAAGTTTAACTTATTTCCAAGGTCGTGGATTAGGTCAATTCTCTCGTGTTTTAT
TAAGTTATCTTGGAATTCCATATGAAAATATTACAGTTACTGAAATTTCAGATGCCCTTC
GTGCAACTCTTCCATATGGTCAATTACCAATTTATCGTGATGGTGATTTTGTTCTTACAC
AATCATCTACCATTGCAAGATACATTGCCAAAAAACACAACTTTATGGGTAAAAATCTTG
AAGAAGAATTTTTAGTTGATCAAATTGTTACTGCAATTCACGCTGATATTTTCCCAGCTT
TTAATAATCCAGTTCCAGAAAAACTCCAAAAACTTTATGAAAAATACTTTGGTTCATTTG
AAAAGAAATTACAAGAAACTGGATTCTTAGTTGGATCATCAGTTACATTAGCTGATTTAT
ATGTTTATGTTGGTTTTGATTACATTAGATTCAGAGGTGAAGCAGCCTTATCCAGTGAAT
TATCAGATGAAAAGTATCCAAAAATCGCTGAATTAAAGAAATTTTTTGAATCAAATGAAG
GTGTAGCAAAATATATTAAAGAAAGACCAGAAACTAAATTTTAAACTGTTTTCATCACCA
TT
Length of 3' end seq. 652
Connected seq. ID VSJ304P
Connected seq. 
>VSJ304P.Seq
AAAAAACTAAACTAAATATTTGAAATGTCAACATCATCAGTCCCAAGTTTAACTTATTTC
CAAGGTCGTGGATTAGGTCAATTCTCTCGTGTTTTATTAAGTTATCTTGGAATTCCATAT
GAAAATATTACAGTTACTGAAATTTCAGATGCCCTTCGTGCAACTCTTCCATATGGTCAA
TTACCAATTTATCGTGATGGTGATTTTGTTCTTACACAATCATCTACCATTGCAAGATAC
ATTGCCAAAAAACACAACTTTATGGGTAAAAATCTTGAAGAAGAATTTTTAGTTGATC--
--------CCACGCGTCCCGAAAAAACTAAANCTAAATATTTGAAATGTCAACATCATCA
GTCCCAAGTTTAACTTATTTCCAAGGTCGTGGATTAGGTCAATTCTCTCGTGTTTTATTA
AGTTATCTTGGAATTCCATATGAAAATATTACAGTTACTGAAATTTCAGATGCCCTTCGT
GCAACTCTTCCATATGGTCAATTACCAATTTATCGTGATGGTGATTTTGTTCTTACACAA
TCATCTACCATTGCAAGATACATTGCCAAAAAACACAACTTTATGGGTAAAAATCTTGAA
GAAGAATTTTTAGTTGATCAAATTGTTACTGCAATTCACGCTGATATTTTCCCAGCTTTT
AATAATCCAGTTCCAGAAAAACTCCAAAAACTTTATGAAAAATACTTTGGTTCATTTGAA
AAGAAATTACAAGAAACTGGATTCTTAGTTGGATCATCAGTTACATTAGCTGATTTATAT
GTTTATGTTGGTTTTGATTACATTAGATTCAGAGGTGAAGCAGCCTTATCCAGTGAATTA
TCAGATGAAAAGTATCCAAAAATCGCTGAATTAAAGAAATTTTTTGAATCAAATGAAGGT
GTAGCAAAATATATTAAAGAAAGACCAGAAACTAAATTTTAAACTGTTTTCATCACCATT
Length of connected seq. 950
Full length Seq ID -
Full length Seq. -
Length of full length seq. -