>VSJ303 (VSJ303Q) /CSM/VS/VSJ3-A/VSJ303Q.Seq.d/
TACCGGGCCCCCCCTCGAGGGAAAAATAATAATAAAAATAATAATAATAATAATTTGAAA
ATGCAAGAAATAAAATTACCAGTACCACCATTTAATAATATTGAAGATGCAATTAAAAAA
GTTAGAATGGCAGAAGATGCATGGAATTCAAGGAATGCAGAAACTGTTTCACTTGCATAC
AGTTTGGATACAAAATGGAGAAATAGGAATGAATTTATTAATGGTAGAACACAAGTTGTT
GAATTTTTAAAAAAGAAATGGGAAAAAGAGGTTGAATATAGACTAATTAAAGAGTTGTGG
ACATTTCAAGCTGGTAGTAATAGAATTGCCGTCAGATTTGCATATGAATGGAAAGATTTA
AATGGTAATTGGTTTAGATCATATGGAAATGAAAATTGGGAATTCAATGAATTTGGTCTA
ATGGTTCAAAGACATGCATCAATAAATGATCTCTCAATTAGTGAATCACAAAGATTATTT
CATTGGCCATGCGGTAAAAGACCTGATGATCATCCAAGTTTATCAGATTTAGGTTTATAA
AAAAATAAAATAAAAAAATAAAAAAATAAAAGAATAAAAAAATAAAATATCXXXXXXXXX
XGAAAAATAATAATAAAAATAATAATAATAATAATTTGAAAATGCAAGAAATAAAATTAC
CAGTACCACCATTTAATAATATTGAAGATGCAATTAAAAAAGTTAGAATGGCAGAAGATG
CATGGAATTCAAGGAATGCAGAAACTGTTTCACTTGCATACAGTTTGGATACAAAATGGA
GAAATAGGAATGAATTTATTAATGGTAGAACACAAGTTGTTGAATTTTTAAAAAAGAAAT
GGGAAAAAGAGGTTGAATATAGACTAATTAAAGAGTTGTGGACATTTCAAGCTGGTAGTA
ATAGAATTGCCGTCAGATTTGCATATGAATGGAAAGATTTAAATGGTAATTGGTTTAGAT
CATATGGAAATGAAAATTGGGAATTCAATGAATTTGGTCTAATGGTTCAAAGACATGCAT
CAATAAATGATCTCTCAATTAGTGAATCACAAAGATTATTTCATTGGCCATGCGGTAAAA
GACCTGATGATCATCCAAGTTTATCAGATTTAGGTTTATAAAAAAATAAAATAAAAAAAT
AAAAAAATAAAAGAATAAAAAAATAAAATAT

pgppsrek***k*****fenarnkitstti**y*rcn*ks*ngrrcmefkecrncftciq
fgykmek*e*iy*w*ntsc*ifkkemgkrg*i*tn*rvvdissw***ncrqici*merfk
w*lv*iiwk*klgiq*iwsngsktcink*sln**itkiislamr*kt**sskfirfrfik
k*nkkikk*KNKKIKY---

---KNNNKNNNNNNLKMQEIKLPVPPFNNIEDAIKKVRMAEDAWNSRNAETVSLAYSLDT
KWRNRNEFINGRTQVVEFLKKKWEKEVEYRLIKELWTFQAGSNRIAVRFAYEWKDLNGNW
FRSYGNENWEFNEFGLMVQRHASINDLSISESQRLFHWPCGKRPDDHPSLSDLGL*knki
kk*knkrikk*n

Frame A:
yrapprgknnnknnnnnnlkmqeiklpvppfnniedaikkvrmaedawnsrnaetvslay
sldtkwrnrnefingrtqvveflkkkwekeveyrlikelwtfqagsnriavrfayewkdl
ngnwfrsygnenwefnefglmvqrhasindlsisesqrlfhwpcgkrpddhpslsdlgl*
knkikk*knkrikk*ni---

---ek***k*****fenarnkitstti**y*rcn*ks*ngrrcmefkecrncftciqfgy
kmek*e*iy*w*ntsc*ifkkemgkrg*i*tn*rvvdissw***ncrqici*merfkw*l
v*iiwk*klgiq*iwsngsktcink*sln**itkiislamr*kt**sskfirfrfikk*n
kkikk*knkkiky

Frame B:
tgpplegkiiikiiiiii*kckk*nyqyhhliilkmqlkklewqkmhgiqgmqklfhlht
vwiqngeigmnllmvehkllnf*krngkkrlnid*lkscghfklvvielpsdlhmngki*
mvigldhmemkignsmnlv*wfkdmhq*misqlvnhkdyfighavkdlmiiqvyqi*vyk
kik*knkkike*knki---

---KNNNKNNNNNNLKMQEIKLPVPPFNNIEDAIKKVRMAEDAWNSRNAETVSLAYSLDT
KWRNRNEFINGRTQVVEFLKKKWEKEVEYRLIKELWTFQAGSNRIAVRFAYEWKDLNGNW
FRSYGNENWEFNEFGLMVQRHASINDLSISESQRLFHWPCGKRPDDHPSLSDLGL*knki
kk*knkrikk*n

Frame C:
pgppsrek***k*****fenarnkitstti**y*rcn*ks*ngrrcmefkecrncftciq
fgykmek*e*iy*w*ntsc*ifkkemgkrg*i*tn*rvvdissw***ncrqici*merfk
w*lv*iiwk*klgiq*iwsngsktcink*sln**itkiislamr*kt**sskfirfrfik
k*nkkikk*KNKKIKY---

---kiiikiiiiii*kckk*nyqyhhliilkmqlkklewqkmhgiqgmqklfhlhtvwiq
ngeigmnllmvehkllnf*krngkkrlnid*lkscghfklvvielpsdlhmngki*mvig
ldhmemkignsmnlv*wfkdmhq*misqlvnhkdyfighavkdlmiiqvyqi*vykkik*
knkkike*knki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ303 (VSJ303Q) /CSM/VS/VSJ3-A/VSJ303Q.Seq.d/                        957   0.0
VSI224 (VSI224Q) /CSM/VS/VSI2-A/VSI224Q.Seq.d/                        957   0.0
VSJ628 (VSJ628Q) /CSM/VS/VSJ6-B/VSJ628Q.Seq.d/                         40   0.075
SSJ540 (SSJ540Q) /CSM/SS/SSJ5-B/SSJ540Q.Seq.d/                         40   0.075
FC-AJ06 (FC-AJ06Q) /CSM/FC/FC-AJ/FC-AJ06Q.Seq.d/                       40   0.075
AFC859 (AFC859Q) /CSM/AF/AFC8-C/AFC859Q.Seq.d/                         40   0.075
SSM717 (SSM717Q) /CSM/SS/SSM7-A/SSM717Q.Seq.d/                         38   0.30
VSJ191 (VSJ191Q) /CSM/VS/VSJ1-D/VSJ191Q.Seq.d/                         36   1.2
VSF739 (VSF739Q) /CSM/VS/VSF7-B/VSF739Q.Seq.d/                         36   1.2
VSF405 (VSF405Q) /CSM/VS/VSF4-A/VSF405Q.Seq.d/                         36   1.2

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

CB671121|CB671121.1 OSJNEe04I14.r OSJNEe Oryza sativa (japonica cultivar-group) cDNA clone OSJNEe04I14 3', mRNA sequence.    40  0.035  2
AL589697|AL589697.1 Human DNA sequence *** SEQUENCING CANCELLED *** from clone  RP11-347H2.    34  0.063  7
AL162587|AL162587.20 Human DNA sequence from clone RP11-280I16 on chromosome 9.    38  0.082  7
U27538|U27538.1 Dictyostelium discoideum leukotriene A4 hydrolase mRNA, partial cds.    36  0.10  3
AC021532|AC021532.6 Homo sapiens chromosome 8 clone RP11-389A13 map 8, WORKING DRAFT SEQUENCE, 3 unordered pieces.    34  0.13  12
AZ523555|AZ523555.1 221PbA02 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    44  0.16  2
BZ561463|BZ561463.1 pacs2-164_3274.x4 pacs2-164 Pseudomonas aeruginosa genomic clone pacs2-164_3274, DNA sequence.    40  0.17  2
AL139379|AL139379.14 Human DNA sequence from clone RP11-358P11 on chromosome 13.    32  0.24  10
AC128842|AC128842.3 Rattus norvegicus clone CH230-463I22, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    48  0.25  3
AL592185|AL592185.35 Mouse DNA sequence from clone RP23-341J22 on chromosome 11.    44  0.33  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000647_3992(CP000647|pid:none) Klebsiella pneumoniae subsp. pn...   233   1e-59
CP000125_1514(CP000125|pid:none) Burkholderia pseudomallei 1710b...   229   2e-58
CP000571_11(CP000571|pid:none) Burkholderia pseudomallei 668 chr...   229   2e-58
CP000085_11(CP000085|pid:none) Burkholderia thailandensis E264 c...   228   2e-58
CP000442_376(CP000442|pid:none) Burkholderia ambifaria AMMD chro...   228   3e-58
CP000094_2876(CP000094|pid:none) Pseudomonas fluorescens Pf0-1, ...   228   4e-58
BX571867_51(BX571867|pid:none) Photorhabdus luminescens subsp. l...   226   9e-58
CP000388_1119(CP000388|pid:none) Pseudoalteromonas atlantica T6c...   225   2e-57
AM039952_4431(AM039952|pid:none) Xanthomonas campestris pv. vesi...   225   2e-57
CP000091_1044(CP000091|pid:none) Ralstonia eutropha JMP134 chrom...   225   2e-57

 psg: 0.31  gvh: 0.24  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VSJ303 is nuc

>VSJ303F.Seq
TACCGGGCCCCCCCTCGAGGGAAAAATAATAATAAAAATAATAATAATAATAATTTGAAA
ATGCAAGAAATAAAATTACCAGTACCACCATTTAATAATATTGAAGATGCAATTAAAAAA
GTTAGAATGGCAGAAGATGCATGGAATTCAAGGAATGCAGAAACTGTTTCACTTGCATAC
AGTTTGGATACAAAATGGAGAAATAGGAATGAATTTATTAATGGTAGAACACAAGTTGTT
GAATTTTTAAAAAAGAAATGGGAAAAAGAGGTTGAATATAGACTAATTAAAGAGTTGTGG
ACATTTCAAGCTGGTAGTAATAGAATTGCCGTCAGATTTGCATATGAATGGAAAGATTTA
AATGGTAATTGGTTTAGATCATATGGAAATGAAAATTGGGAATTCAATGAATTTGGTCTA
ATGGTTCAAAGACATGCATCAATAAATGATCTCTCAATTAGTGAATCACAAAGATTATTT
CATTGGCCATGCGGTAAAAGACCTGATGATCATCCAAGTTTATCAGATTTAGGTTTATAA
AAAAATAAAATAAAAAAATAAAAAAATAAAAGAATAAAAAAATAAAATATC---------
-

>VSJ303Z.Seq
----------GAAAAATAATAATAAAAATAATAATAATAATAATTTGAAAATGCAAGAAA
TAAAATTACCAGTACCACCATTTAATAATATTGAAGATGCAATTAAAAAAGTTAGAATGG
CAGAAGATGCATGGAATTCAAGGAATGCAGAAACTGTTTCACTTGCATACAGTTTGGATA
CAAAATGGAGAAATAGGAATGAATTTATTAATGGTAGAACACAAGTTGTTGAATTTTTAA
AAAAGAAATGGGAAAAAGAGGTTGAATATAGACTAATTAAAGAGTTGTGGACATTTCAAG
CTGGTAGTAATAGAATTGCCGTCAGATTTGCATATGAATGGAAAGATTTAAATGGTAATT
GGTTTAGATCATATGGAAATGAAAATTGGGAATTCAATGAATTTGGTCTAATGGTTCAAA
GACATGCATCAATAAATGATCTCTCAATTAGTGAATCACAAAGATTATTTCATTGGCCAT
GCGGTAAAAGACCTGATGATCATCCAAGTTTATCAGATTTAGGTTTATAAAAAAATAAAA
TAAAAAAATAAAAAAATAAAAGAATAAAAAAATAAAATAT

>VSJ303P.Seq
TACCGGGCCCCCCCTCGAGGGAAAAATAATAATAAAAATAATAATAATAATAATTTGAAA
ATGCAAGAAATAAAATTACCAGTACCACCATTTAATAATATTGAAGATGCAATTAAAAAA
GTTAGAATGGCAGAAGATGCATGGAATTCAAGGAATGCAGAAACTGTTTCACTTGCATAC
AGTTTGGATACAAAATGGAGAAATAGGAATGAATTTATTAATGGTAGAACACAAGTTGTT
GAATTTTTAAAAAAGAAATGGGAAAAAGAGGTTGAATATAGACTAATTAAAGAGTTGTGG
ACATTTCAAGCTGGTAGTAATAGAATTGCCGTCAGATTTGCATATGAATGGAAAGATTTA
AATGGTAATTGGTTTAGATCATATGGAAATGAAAATTGGGAATTCAATGAATTTGGTCTA
ATGGTTCAAAGACATGCATCAATAAATGATCTCTCAATTAGTGAATCACAAAGATTATTT
CATTGGCCATGCGGTAAAAGACCTGATGATCATCCAAGTTTATCAGATTTAGGTTTATAA
AAAAATAAAATAAAAAAATAAAAAAATAAAAGAATAAAAAAATAAAATATC---------
-GAAAAATAATAATAAAAATAATAATAATAATAATTTGAAAATGCAAGAAATAAAATTAC
CAGTACCACCATTTAATAATATTGAAGATGCAATTAAAAAAGTTAGAATGGCAGAAGATG
CATGGAATTCAAGGAATGCAGAAACTGTTTCACTTGCATACAGTTTGGATACAAAATGGA
GAAATAGGAATGAATTTATTAATGGTAGAACACAAGTTGTTGAATTTTTAAAAAAGAAAT
GGGAAAAAGAGGTTGAATATAGACTAATTAAAGAGTTGTGGACATTTCAAGCTGGTAGTA
ATAGAATTGCCGTCAGATTTGCATATGAATGGAAAGATTTAAATGGTAATTGGTTTAGAT
CATATGGAAATGAAAATTGGGAATTCAATGAATTTGGTCTAATGGTTCAAAGACATGCAT
CAATAAATGATCTCTCAATTAGTGAATCACAAAGATTATTTCATTGGCCATGCGGTAAAA
GACCTGATGATCATCCAAGTTTATCAGATTTAGGTTTATAAAAAAATAAAATAAAAAAAT
AAAAAAATAAAAGAATAAAAAAATAAAATAT