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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSJ243
Library VS
(Link to library)
Clone ID VSJ243
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14349-1
Original site URL
Representative seq. ID VSJ243P
(Link to Original site)
Representative DNA sequence 
>VSJ243 (VSJ243Q) /CSM/VS/VSJ2-B/VSJ243Q.Seq.d/
AAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAAC
CTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGA
TTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCAC
CAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGA
AGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAA
AAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCA
AGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATG
TACCAAAATCTAAATAATTTGAAAAAATTTAAAAAAAAAAXXXXXXXXXXAAAAAACTAT
ATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAACCTTCAACGAT
TTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGATTCAAAAGAA
ATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCACCAAATGTCTC
CCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGAAGGTGCCCAA
AAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAAAAAGAAAATG
ATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATC
CAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATAAAAATGTACCAAAATCT
AAATAATT
sequence update 2001. 3.22
Translated Amino Acid sequence 
kklyiknvfrycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlfir*ih
qmsprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*slcwyp
s*npryrcl*sqrfmfl*KMYQNLNNLKKFKKK---

---KKLYIKNVFRYCFSSKLRFNLQRFKVRS*iwwyhlqnl*rfkrnhc*lhltswlfir
*ihqmsprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*slc
wyps*npryrcl*sqrfmfl*kctki*ii
Translated Amino Acid sequence (All Frames) 
Frame A:
kklyiknvfrycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlfir*ih
qmsprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*slcwyp
s*npryrcl*sqrfmfl*KMYQNLNNLKKFKKK---

---KKLYIKNVFRYCFSSKLRFNLQRFKVRS*iwwyhlqnl*rfkrnhc*lhltswlfir
*ihqmsprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*slc
wyps*npryrcl*sqrfmfl*kctki*ii

Frame B:
knyi*kmssgialapncvstfndlklgrkyggiiyrisddskeiivdstlpagcsfdeft
kclpenecryvvldyqykeegaqkskicfvawcpdtanikkkmmatsskdslrkacvgiq
veiqgtdasevkdscfyekctki*ii*knlkkk---

---knyi*kmssgialapncvstfndlklgrkyggiiyrisddskeiivdstlpagcsfd
eftkclpenecryvvldyqykeegaqkskicfvawcpdtanikkkmmatsskdslrkacv
giqveiqgtdasevkdscfyknvpksk*

Frame C:
ktiykkclqvll*lqtafqpsti*s*vvnmvvsstesltiqkkslltppyqlvvhsmnsp
nvsqktnvdtlsstintkkkvpkraksvllpgaqipptlkrk*wpphqktlyvkpvlvsk
lkskvpmplkskihvsmknvpksk*feki*kk---

---ktiykkclqvll*lqtafqpsti*s*vvnmvvsstesltiqkkslltppyqlvvhsm
nspnvsqktnvdtlsstintkkkvpkraksvllpgaqipptlkrk*wpphqktlyvkpvl
vsklkskvpmplkskihvsikmyqnlnn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ890 (VSJ890Q) /CSM/VS/VSJ8-D/VSJ890Q.Seq.d/                        874   0.0
VSJ584 (VSJ584Q) /CSM/VS/VSJ5-D/VSJ584Q.Seq.d/                        874   0.0
VSJ550 (VSJ550Q) /CSM/VS/VSJ5-C/VSJ550Q.Seq.d/                        874   0.0
VSJ437 (VSJ437Q) /CSM/VS/VSJ4-B/VSJ437Q.Seq.d/                        874   0.0
VSJ243 (VSJ243Q) /CSM/VS/VSJ2-B/VSJ243Q.Seq.d/                        874   0.0
VSG665 (VSG665Q) /CSM/VS/VSG6-C/VSG665Q.Seq.d/                        874   0.0
VSD383 (VSD383Q) /CSM/VS/VSD3-D/VSD383Q.Seq.d/                        874   0.0
VFL333 (VFL333Q) /CSM/VF/VFL3-B/VFL333Q.Seq.d/                        874   0.0
VFE845 (VFE845Q) /CSM/VF/VFE8-B/VFE845Q.Seq.d/                        874   0.0
VFA592 (VFA592Q) /CSM/VF/VFA5-D/VFA592Q.Seq.d/                        874   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D37981|D37981.1 Dictyostelium discoidium DDCOF2 gene for cofilin, complete cds.   874  0.0  2
D37980|D37980.1 Dictyostelium discoidium DDCOF1 gene for cofilin, complete cds (exon1-2).   833  0.0  3
X04004|X04004.1 Dictyostelium discoideum mRNA for contact site A (csA) protein.   168  8e-38  1
BM416516|BM416516.1 OP21612 Mixed Stage EST's from Globodera pallida, the potato cyst nematode Globodera pallida cDNA, mRNA sequence.    40  5e-04  2
AC069574|AC069574.1 Homo sapiens chromosome 4 clone RP11-267H10 map 4, LOW-PASS SEQUENCE SAMPLING.    40  0.026  2
AC143994|AC143994.1 Macaca mulatta clone CH250-272N23, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    38  0.070  3
AQ866926|AQ866926.1 nbeb0029D05f CUGI Rice BAC Library (EcoRI) Oryza sativa genomic clone nbeb0029D05f, genomic survey sequence.    40  0.47  2
AQ860777|AQ860777.1 nbeb0015B18r CUGI Rice BAC Library (EcoRI) Oryza sativa genomic clone nbeb0015B18r, genomic survey sequence.    36  0.62  2
AQ861500|AQ861500.1 nbeb0017A09r CUGI Rice BAC Library (EcoRI) Oryza sativa genomic clone nbeb0017A09r, genomic survey sequence.    34  0.63  2
AJ511931|AJ511931.1 Apodemus sylvaticus mitochondrial partial cytb gene for cytochrome  b, tissue library JRM-417.    46  0.75  1
dna update 2003. 7.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54706) RecName: Full=Cofilin-1;  &D37980_1(D37980|pid:none) &D...   280   3e-74
(P37167) RecName: Full=Actophorin;  &M93361_1(M93361|pid:none)        130   6e-29
FM992692_38(FM992692|pid:none) Candida dubliniensis CD36 chromos...   125   2e-27
(Q96VU9) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   125   2e-27
AY428595_1(AY428595|pid:none) Helianthus annuus clone HA10-7 put...   123   7e-27
(Q9HF97) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   121   3e-26
CP000501_299(CP000501|pid:none) Pichia stipitis CBS 6054 chromos...   120   8e-26
(Q6CQ22) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   119   2e-25
(Q03048) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   116   9e-25
EU961226_1(EU961226|pid:none) Zea mays clone 233661 actin-depoly...   116   9e-25
protein update 2009. 3.25
PSORT 

 psg: 0.36  gvh: 0.34  alm: 0.58  top: 0.53  tms: 0.00  mit: 0.56  mip: 0.00
 nuc: 0.32  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 20.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: endoplasmic reticulum

>> prediction for VSJ243 is nuc
5' end seq. ID VSJ243F
5' end seq. 
>VSJ243F.Seq
AAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAAC
CTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGA
TTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCAC
CAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGA
AGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAA
AAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCA
AGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATG
TACCAAAATCTAAATAATTTGAAAAAATTTAAAAAAAAAA----------
Length of 5' end seq. 460
3' end seq. ID VSJ243Z
3' end seq. 
>VSJ243Z.Seq
----------AAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACT
GCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAA
TCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCG
ATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAAT
ACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCG
CCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTG
TTGGTATCCAAGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCT
ATAAAAATGTACCAAAATCTAAATAATT
Length of 3' end seq. 438
Connected seq. ID VSJ243P
Connected seq. 
>VSJ243P.Seq
AAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAAC
CTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGA
TTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCAC
CAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGA
AGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAA
AAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCA
AGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATG
TACCAAAATCTAAATAATTTGAAAAAATTTAAAAAAAAAA----------AAAAAACTAT
ATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAACCTTCAACGAT
TTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGATTCAAAAGAA
ATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCACCAAATGTCTC
CCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGAAGGTGCCCAA
AAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAAAAAGAAAATG
ATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATC
CAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATAAAAATGTACCAAAATCT
AAATAATT
Length of connected seq. 898
Full length Seq ID -
Full length Seq. -
Length of full length seq. -