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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSJ209
Library VS
(Link to library)
Clone ID VSJ209
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VSJ209E
(Link to Original site)
Representative DNA sequence 
>VSJ209 (VSJ209Q) /CSM/VS/VSJ2-A/VSJ209Q.Seq.d/
GAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGT
ACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTC
AAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGC
TCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGA
TACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATG
TGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGA
TTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCAAGATTCCGTCAC
TGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTAT
TGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTNTNN
NNAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACAC
TCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTT
CAATCGATAACACCAAANACACTGGTGACAATTACCTACGTCCCATTAACCAACGAAACC
TATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGT
ACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATAGCTGAT
ATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCT
GATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTT
GTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTG
AGTGGATTTATGGGTATCGAGTTAAACATGGNNAATTTCTGGATCCTTGGTGATGTTTTC
ATCTCTGCANTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGC
CATTCAAGGTTAAATTTTTAGT
sequence update 2001. 3.26
Translated Amino Acid sequence 
KLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTIPISDFEDA
QYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGASSTYVANGTD
FTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGILGLAFQSIXX
XFNSTSLLQHVITRFSFINTLLLLVIKNSRCQRW*tlirfnr*hqxhw*qlptsh*ptkp
ignslwmtllsmvnqlvsvvllvtqfaiqvhhsllvq*lillpsmkn*vlss*mvkvssl
ivalstpyqmlpspllvvnlf*lqkntf*kllsserlnv*vdlwvss*twxisgslvmfs
slxyytvfdfgnkqvgfataiqg*ifs
Translated Amino Acid sequence (All Frames) 
Frame A:
eityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwyhnpnfrf*rc
silwchyhwyprsslqssfrywfiqlvdsikemsnhcccm*ft*qi*qrclkhicrqrn*
fhhpir*wcyvrfclsrfrhcwfinc*rsiir*shcrtrycfrfrqirwyfrscfpihlx
xiqfhqsfttcyhkv*fhqhsspsgyqelqvptvvnshsvqsitpxtlvtityvpltnet
ywefvmddfaidgqsagfcgttchaicdsgtsliagpiaditalneklgavilngegvfs
dcsvintlpnvtitvagrefvltpkeyvlevtefgkteclsgfmgielnmxnfwilgdvf
isaxlycirfw**tswfrnchsrlnf*


Frame B:
KLLILTLFLATIVLAQALTVPLNFHQASRESRRRVPQKWSNRLSALNAGTTIPISDFEDA
QYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCPITVVACDLHNKYNSGASSTYVANGTD
FTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAEATAEPGIAFDFAKFDGILGLAFQSIXX
XFNSTSLLQHVITRFSFINTLLLLVIKNSRCQRW*tlirfnr*hqxhw*qlptsh*ptkp
ignslwmtllsmvnqlvsvvllvtqfaiqvhhsllvq*lillpsmkn*vlss*mvkvssl
ivalstpyqmlpspllvvnlf*lqkntf*kllsserlnv*vdlwvss*twxisgslvmfs
slxyytvfdfgnkqvgfataiqg*ifs


Frame C:
nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyllsmlvpqsqfqilkml
ntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslllhviyitnitavpqahmspteli
spsntvvvlcqvlslkipsllvh*llkinyslkplpnqvllsispnsmvf*vllsnpsxx
nsippvfynmlsqglvsstlfsfwlsrtpganggelsfgsidntkxtgdnylrpinqrnl
lgiryg*lcyrwsiswflwyylsrnlrfryithcwsns*yycpq*kircchlkw*rcll*
l*ryqhltkcyhhrcws*icfnskrirfrsy*vrkd*mfewiygyrvkhgxfldpw*cfh
lcxtilysilvinklvsqlpfkvkfl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ209 (VSJ209Q) /CSM/VS/VSJ2-A/VSJ209Q.Seq.d/                       2250   0.0
VFN380 (VFN380Q) /CSM/VF/VFN3-D/VFN380Q.Seq.d/                       2194   0.0
VFN222 (VFN222Q) /CSM/VF/VFN2-A/VFN222Q.Seq.d/                       2194   0.0
VFM881 (VFM881Q) /CSM/VF/VFM8-D/VFM881Q.Seq.d/                       2194   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       2194   0.0
VFM234 (VFM234Q) /CSM/VF/VFM2-B/VFM234Q.Seq.d/                       2194   0.0
VFL410 (VFL410Q) /CSM/VF/VFL4-A/VFL410Q.Seq.d/                       2194   0.0
VFL385 (VFL385Q) /CSM/VF/VFL3-D/VFL385Q.Seq.d/                       2194   0.0
VFL274 (VFL274Q) /CSM/VF/VFL2-D/VFL274Q.Seq.d/                       2194   0.0
VFL252 (VFL252Q) /CSM/VF/VFL2-C/VFL252Q.Seq.d/                       2194   0.0
own update 2004. 8. 9
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.  1063  0.0  4
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.   950  0.0  5
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62  2e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62  2e-05  1
AF372974|AF372974.1 Arabidopsis thaliana AT4g04460/T26N6_7 mRNA, complete cds.    62  2e-05  1
BE525959|BE525959.1 M64C07STM Arabidopsis developing seed Arabidopsis thaliana cDNA clone 600034526R1 5', mRNA sequence.    62  2e-05  1
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62  2e-05  1
AL765461|AL765461.1 Arabidopsis thaliana T-DNA flanking sequence GK-139E08-012875.    62  2e-05  1
E33916|E33916.1 Candida boidinii strain with lowered protease activity and  utilization thereof as host for producing foreign protein.    36  4e-05  4
CB814835|CB814835.1 USDA-FP_100247 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-04_A10 5', mRNA sequence.    36  4e-05  3
dna update 2003. 7.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   179   7e-87
EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   183   4e-85
DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   184   1e-84
EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   178   9e-84
FJ168036_1(FJ168036|pid:none) Fasciola hepatica cathepsin D-like...   172   1e-81
DQ909010_1(DQ909010|pid:none) Clonorchis sinensis aspartic prote...   170   2e-81
U90750_1(U90750|pid:none) Schistosoma japonicum aspartic proteas...   172   7e-80
L41346_1(L41346|pid:none) Schistosoma japonicum aspartic protein...   172   7e-80
EF000001_1(EF000001|pid:none) Fasciola hepatica cathepsin D-like...   172   1e-79
U60995_1(U60995|pid:none) Schistosoma mansoni aspartic proteinas...   172   6e-79
protein update 2009. 3.25
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.33  top: 0.70  tms: 0.00  mit: 0.37  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 24.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: nuclear
	  4.0 %: endoplasmic reticulum

>> prediction for VSJ209 is exc
5' end seq. ID VSJ209F
5' end seq. 
>VSJ209F.Seq
GAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGT
ACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTC
AAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGC
TCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGA
TACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATG
TGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGA
TTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCAAGATTCCGTCAC
TGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTAT
TGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTCTGT
TA----------
Length of 5' end seq. 542
3' end seq. ID VSJ209Z
3' end seq. 
>VSJ209Z.Seq
----------TATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCAAA
TTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTATTGTTAATTCAATTCCACCAGT
CTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATC
AAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAANA
CACTGGTGACAATTACCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGG
ATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTT
GCGATTCAGGTACATCACTCATTGCTGGTCCAATAGCTGATATTACTGCCCTCAATGAAA
AATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACA
CCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAAT
ACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCG
AGTTAAACATGGNNAATTTCTGGATCCTTGGTGATGTTTTCATCTCTGCANTACTATACT
GTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTT
AGT
Length of 3' end seq. 713
Connected seq. ID VSJ209P
Connected seq. 
>VSJ209P.Seq
GAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGT
ACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTC
AAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGC
TCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGA
TACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATG
TGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGA
TTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCAAGATTCCGTCAC
TGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTAT
TGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTCTGT
TA----------TATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTTTCGATTTCGCCA
AATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTATTGTTAATTCAATTCCACCA
GTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTA
TCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAATCGATAACACCAAA
NACACTGGTGACAATTACCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTAT
GGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAAT
TTGCGATTCAGGTACATCACTCATTGCTGGTCCAATAGCTGATATTACTGCCCTCAATGA
AAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAA
CACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGA
ATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTAT
CGAGTTAAACATGGNNAATTTCTGGATCCTTGGTGATGTTTTCATCTCTGCANTACTATA
CTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTCAAGGTTAAATTT
TTAGT
Length of connected seq. 1255
Full length Seq ID VSJ209E
Full length Seq. 
>VSJ209E.Seq
GAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTTTAGCTCAAGCTTTAACAGT
ACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAGAGTTCCACAAAAATGGTC
AAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAATTTCAGATTTTGAAGATGC
TCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAGCCTTCAAAGTAGTTTTCGA
TACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTCCAATCACTGTTGTTGCATG
TGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACATATGTCGCCAACGGAACTGA
TTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTTGTCTCTCAAGATTCCGTCAC
TGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTAT
TGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTNTNN
NNAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACAC
TCTTCTCCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTT
CAATCGATAACACCAAANACACTGGTGACAATTACCTACGTCCCATTAACCAACGAAACC
TATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGT
ACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATAGCTGAT
ATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCT
GATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTT
GTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTG
AGTGGATTTATGGGTATCGAGTTAAACATGGNNAATTTCTGGATCCTTGGTGATGTTTTC
ATCTCTGCANTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGC
CATTCAAGGTTAAATTTTTAGT
Length of full length seq. 1162