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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSJ148
Library VS
(Link to library)
Clone ID VSJ148
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSJ148P
(Link to Original site)
Representative DNA sequence 
>VSJ148 (VSJ148Q) /CSM/VS/VSJ1-B/VSJ148Q.Seq.d/
GATNAACCAACAACGCGTAACTATAAAATGCCTTCAGTACAAAAAACCGTCGTAAAGAAA
TCACATAAATTCGTCATTGATTGTACCGCCCCAGCCGGTAAAATCGTCGATGTTGCTGCT
TTCGAAAAGTACTTACACGATAGAATCAAAGTTGACAACAAAGTTGGTAACCTCGGTTCA
ACTGTTGTTATCTCAAAAGATAAATCAAAGATCATCATCAACACCACCATCCCATTCTCA
AAGAGATACCTCAAATATTTAACCAAGAAATTCTTAAAATTCAAACAAATCAGAGATTTC
TTACGTGTTGTTGCCACCACCAAAAACACCTACGAACTCAGATACTTCAACATTGGTGAC
TCTGAATCACAAGAATAAACACTTTGGTCCCAACCTTTGATCAAAATGGTAAACGAATAA
ATCTGGTTTAACATTTAAAAAAAAAAXXXXXXXXXXGATCAAACCAACAACGCGTAACTA
TAAAATGCCTTCAGTACAAAAAACCGTCGTAAAGAAATCACATAAATTCGTCATTGATTG
TACCGCCCCAGCCGGTAAAATCGTCGATGTTGCTGCTTTCGAAAAGTACTTACACGATAG
AATCAAAGTTGACAACAAAGTTGGTAACCTCGGTTCAACTGTTGTTATCTCAAAAGATAA
ATCAAAGATCATCATCAACACCACCATCCCATTCTCAAAGAGATACCTCAAATATTTAAC
CAAGAAATTCTTAAAATTCAAACAAATCAGAGATTTCTTACGTGTTGTTGCCACCACCAA
AAACACCTACGAACTCAGATACTTCAACATTGGTGACTCTGAATCACAAGAATAAACACT
TTGGTCCCAACCTTNATCAAAANGTAAACGAATAAATCGGGTTTAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
xnqqrvtikclqykkps*rnhinsslivppqpvkssmlllskstytiesklttklvtsvq
lllsqkinqrssstppshsqrdtsni*prns*nsnkseisyvllpppktptnsdtstlvt
lnhknkhfgpnl*skw*TNKSGLTFKKK---

---IKPTTRNYKMPSVQKTVVKKSHKFVIDCTAPAGKIVDVAAFEKYLHDRIKVDNKVGN
LGSTVVISKDKSKIIINTTIPFSKRYLKYLTKKFLKFKQIRDFLRVVATTKNTYELRYFN
IGDSESQE*tlwsqpxskxkrinrv*
Translated Amino Acid sequence (All Frames) 
Frame A:
dxpttrnykmpsvqktvvkkshkfvidctapagkivdvaafekylhdrikvdnkvgnlgs
tvviskdkskiiinttipfskrylkyltkkflkfkqirdflrvvattkntyelryfnigd
sesqe*tlwsqplikmvne*iwfni*kk---

---dqtnna*l*nafstknrrkeit*irh*lyrpsr*nrrcccfrkvltr*nqs*qqsw*
prfnccylkr*ikdhhqhhhpilkeipqifnqeilkiqtnqrfltccchhqkhlrtqilq
hw*l*itrintlvptxikx*tnksgl

Frame B:
xnqqrvtikclqykkps*rnhinsslivppqpvkssmlllskstytiesklttklvtsvq
lllsqkinqrssstppshsqrdtsni*prns*nsnkseisyvllpppktptnsdtstlvt
lnhknkhfgpnl*skw*TNKSGLTFKKK---

---IKPTTRNYKMPSVQKTVVKKSHKFVIDCTAPAGKIVDVAAFEKYLHDRIKVDNKVGN
LGSTVVISKDKSKIIINTTIPFSKRYLKYLTKKFLKFKQIRDFLRVVATTKNTYELRYFN
IGDSESQE*tlwsqpxskxkrinrv*

Frame C:
xtnna*l*nafstknrrkeit*irh*lyrpsr*nrrcccfrkvltr*nqs*qqsw*prfn
ccylkr*ikdhhqhhhpilkeipqifnqeilkiqtnqrfltccchhqkhlrtqilqhw*l
*itrintlvptfdqngkrinlv*hlkkk---

---snqqrvtikclqykkps*rnhinsslivppqpvkssmlllskstytiesklttklvt
svqlllsqkinqrssstppshsqrdtsni*prns*nsnkseisyvllpppktptnsdtst
lvtlnhknkhfgpnlxqxvne*igf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ148 (VSJ148Q) /CSM/VS/VSJ1-B/VSJ148Q.Seq.d/                        858   0.0
VSI831 (VSI831Q) /CSM/VS/VSI8-B/VSI831Q.Seq.d/                        856   0.0
FC-219 (FC-219Q) /CSM/CG/CG-FC/FC-219Q.Seq.d/                         856   0.0
FC-AM01 (FC-AM01Q) /CSM/FC/FC-AM/FC-AM01Q.Seq.d/                      846   0.0
VSF652 (VSF652Q) /CSM/VS/VSF6-C/VSF652Q.Seq.d/                        844   0.0
VHI259 (VHI259Q) /CSM/VH/VHI2-C/VHI259Q.Seq.d/                        842   0.0
FC-AT11 (FC-AT11Q) /CSM/FC/FC-AT/FC-AT11Q.Seq.d/                      837   0.0
VSF864 (VSF864Q) /CSM/VS/VSF8-C/VSF864Q.Seq.d/                        833   0.0
FC-BM18 (FC-BM18Q) /CSM/FC/FC-BM/FC-BM18Q.Seq.d/                      825   0.0
VSG473 (VSG473Q) /CSM/VS/VSG4-D/VSG473Q.Seq.d/                        803   0.0
own update 2003. 1.10
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AU114209|AU114209.1 Caenorhabditis elegans cDNA clone:yk716d8 : 3' end, single read.    42  1e-05  2
AV187840|AV187840.1 Caenorhabditis elegans cDNA clone:yk516e11 : 5' end, single read.    42  1e-05  2
AV201830|AV201830.1 Caenorhabditis elegans cDNA clone:yk542b5 : 5' end, single read.    42  4e-04  2
AV187261|AV187261.1 Caenorhabditis elegans cDNA clone:yk509f2 : 5' end, single read.    42  6e-04  2
C68387|C68387.1 C.elegans cDNA clone yk302a9 : 5' end, single read.    42  6e-04  2
C71228|C71228.1 C.elegans cDNA clone yk442e8 : 5' end, single read.    42  7e-04  2
AV191587|AV191587.1 Caenorhabditis elegans cDNA clone:yk595f3 : 5' end, single read.    42  7e-04  2
AV197144|AV197144.1 Caenorhabditis elegans cDNA clone:yk662f8 : 5' end, single read.    42  7e-04  2
AV193941|AV193941.1 Caenorhabditis elegans cDNA clone:yk623d2 : 5' end, single read.    42  7e-04  2
AV202078|AV202078.1 Caenorhabditis elegans cDNA clone:yk545d8 : 5' end, single read.    42  7e-04  2
dna update 2003. 7.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54JE3) RecName: Full=60S ribosomal protein L22 1;                   156   9e-37
(Q54GK6) RecName: Full=60S ribosomal protein L22 2;                   152   1e-35
AF542529_8(AF542529|pid:none) Cryptococcus neoformans var. grubi...    84   5e-15
AF542530_16(AF542530|pid:none) Cryptococcus neoformans var. neof...    84   5e-15
AY710429_17(AY710429|pid:none) Cryptococcus gattii strain E566 M...    83   1e-14
AY710430_6(AY710430|pid:none) Cryptococcus gattii strain WM276 M...    81   4e-14
(P50886) RecName: Full=60S ribosomal protein L22;  &(Q28IL6) Rec...    79   1e-13
AF548320_1(AF548320|pid:none) Branchiostoma belcheri ribosomal p...    78   4e-13
AM270020_3(AM270020|pid:none) Aspergillus niger contig An02c0250...    77   6e-13
EF085347_1(EF085347|pid:none) Picea sitchensis clone WS02713_C14...    77   9e-13
protein update 2009. 3.25
PSORT 

 psg: 0.53  gvh: 0.25  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.40  mip: 0.04
 nuc: 0.12  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal

>> prediction for VSJ148 is nuc
5' end seq. ID VSJ148F
5' end seq. 
>VSJ148F.Seq
GATNAACCAACAACGCGTAACTATAAAATGCCTTCAGTACAAAAAACCGTCGTAAAGAAA
TCACATAAATTCGTCATTGATTGTACCGCCCCAGCCGGTAAAATCGTCGATGTTGCTGCT
TTCGAAAAGTACTTACACGATAGAATCAAAGTTGACAACAAAGTTGGTAACCTCGGTTCA
ACTGTTGTTATCTCAAAAGATAAATCAAAGATCATCATCAACACCACCATCCCATTCTCA
AAGAGATACCTCAAATATTTAACCAAGAAATTCTTAAAATTCAAACAAATCAGAGATTTC
TTACGTGTTGTTGCCACCACCAAAAACACCTACGAACTCAGATACTTCAACATTGGTGAC
TCTGAATCACAAGAATAAACACTTTGGTCCCAACCTTTGATCAAAATGGTAAACGAATAA
ATCTGGTTTAACATTTAAAAAAAAAA----------
Length of 5' end seq. 446
3' end seq. ID VSJ148Z
3' end seq. 
>VSJ148Z.Seq
----------GATCAAACCAACAACGCGTAACTATAAAATGCCTTCAGTACAAAAAACCG
TCGTAAAGAAATCACATAAATTCGTCATTGATTGTACCGCCCCAGCCGGTAAAATCGTCG
ATGTTGCTGCTTTCGAAAAGTACTTACACGATAGAATCAAAGTTGACAACAAAGTTGGTA
ACCTCGGTTCAACTGTTGTTATCTCAAAAGATAAATCAAAGATCATCATCAACACCACCA
TCCCATTCTCAAAGAGATACCTCAAATATTTAACCAAGAAATTCTTAAAATTCAAACAAA
TCAGAGATTTCTTACGTGTTGTTGCCACCACCAAAAACACCTACGAACTCAGATACTTCA
ACATTGGTGACTCTGAATCACAAGAATAAACACTTTGGTCCCAACCTTNATCAAAANGTA
AACGAATAAATCGGGTTTAA
Length of 3' end seq. 430
Connected seq. ID VSJ148P
Connected seq. 
>VSJ148P.Seq
GATNAACCAACAACGCGTAACTATAAAATGCCTTCAGTACAAAAAACCGTCGTAAAGAAA
TCACATAAATTCGTCATTGATTGTACCGCCCCAGCCGGTAAAATCGTCGATGTTGCTGCT
TTCGAAAAGTACTTACACGATAGAATCAAAGTTGACAACAAAGTTGGTAACCTCGGTTCA
ACTGTTGTTATCTCAAAAGATAAATCAAAGATCATCATCAACACCACCATCCCATTCTCA
AAGAGATACCTCAAATATTTAACCAAGAAATTCTTAAAATTCAAACAAATCAGAGATTTC
TTACGTGTTGTTGCCACCACCAAAAACACCTACGAACTCAGATACTTCAACATTGGTGAC
TCTGAATCACAAGAATAAACACTTTGGTCCCAACCTTTGATCAAAATGGTAAACGAATAA
ATCTGGTTTAACATTTAAAAAAAAAA----------GATCAAACCAACAACGCGTAACTA
TAAAATGCCTTCAGTACAAAAAACCGTCGTAAAGAAATCACATAAATTCGTCATTGATTG
TACCGCCCCAGCCGGTAAAATCGTCGATGTTGCTGCTTTCGAAAAGTACTTACACGATAG
AATCAAAGTTGACAACAAAGTTGGTAACCTCGGTTCAACTGTTGTTATCTCAAAAGATAA
ATCAAAGATCATCATCAACACCACCATCCCATTCTCAAAGAGATACCTCAAATATTTAAC
CAAGAAATTCTTAAAATTCAAACAAATCAGAGATTTCTTACGTGTTGTTGCCACCACCAA
AAACACCTACGAACTCAGATACTTCAACATTGGTGACTCTGAATCACAAGAATAAACACT
TTGGTCCCAACCTTNATCAAAANGTAAACGAATAAATCGGGTTTAA
Length of connected seq. 876
Full length Seq ID -
Full length Seq. -
Length of full length seq. -