>VSJ146 (VSJ146Q) /CSM/VS/VSJ1-B/VSJ146Q.Seq.d/
AAGAAGAGAAAGAAGAGAAAGTCTGACTACACCTCATTCTCAACCTACATCCACAAGTTA
TTGAAACAAATTACTCCACCAACCAATGCCAAGAGTAATGAAAAAGGTGATAGAAAATTC
ACTATCTCATCCAAAGCCATGTCTGTAATGAACTCCTTTGTCCACGATATCTTTGACAGA
ATCGCCACCGAAGCTTCTGGTTTAGCCAAAAAGAAGAAGAGACAAACTTTACACTCAAGA
GATATTCAAGTTGCCGTTCGTATCATTCTCACTGGTGAGTTAGCTAAACATGCCATCTTA
CAAGGTATGACTGCTGTCAACAAGTACAATCCAACTGAAAGCAAAAACTAAATTGGTTCC
AAATGAACTATATTCCTTAAAAAAATATAGTTTCCAATTTTCTTTTTTTTTTGNACTCTC
TCTATCATTCTTTTTTAAAAAAAAAAAANTCAAAAAAAAAAXXXXXXXXXX

KKRKKRKSDYTSFSTYIHKLLKQITPPTNAKSNEKGDRKFTISSKAMSVMNSFVHDIFDR
IATEASGLAKKKKRQTLHSRDIQVAVRIILTGELAKHAILQGMTAVNKYNPTESKN*igs
k*tiflkki*fpiffffxtlsiilf*kkkxqkk---

Frame A:
KKRKKRKSDYTSFSTYIHKLLKQITPPTNAKSNEKGDRKFTISSKAMSVMNSFVHDIFDR
IATEASGLAKKKKRQTLHSRDIQVAVRIILTGELAKHAILQGMTAVNKYNPTESKN*igs
k*tiflkki*fpiffffxtlsiilf*kkkxqkk---


Frame B:
rrerreslttphsqptstsy*nkllhqpmprvmkkvienslshpkpcl**tplstislte
sppkllv*pkrrrdklytqeifklpfvsfslvs*lnmpsykv*llststiqlkaktklvp
nelyslkkysfqfsfffxlslsfffkkkkxkkk---


Frame C:
eekeekv*lhlilnlhpqvietnystnqcqe**kr**kihyliqshvcnellcpryl*qn
rhrsfwfsqkeeetnftlkrysscrsyhshw*vs*tchltrydccqqvqsn*kqklnwfq
mnyip*knivsnflfflxslyhsflkkkxskk---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ146 (VSJ146Q) /CSM/VS/VSJ1-B/VSJ146Q.Seq.d/                        799   0.0
VSE878 (VSE878Q) /CSM/VS/VSE8-D/VSE878Q.Seq.d/                        799   0.0
VSE742 (VSE742Q) /CSM/VS/VSE7-B/VSE742Q.Seq.d/                        799   0.0
VSB637 (VSB637Q) /CSM/VS/VSB6-B/VSB637Q.Seq.d/                        799   0.0
VSB542 (VSB542Q) /CSM/VS/VSB5-B/VSB542Q.Seq.d/                        799   0.0
VSB150 (VSB150Q) /CSM/VS/VSB1-C/VSB150Q.Seq.d/                        799   0.0
VSA778 (VSA778Q) /CSM/VS/VSA7-D/VSA778Q.Seq.d/                        799   0.0
VSA488 (VSA488Q) /CSM/VS/VSA4-D/VSA488Q.Seq.d/                        799   0.0
SSK162 (SSK162Q) /CSM/SS/SSK1-C/SSK162Q.Seq.d/                        799   0.0
SSK152 (SSK152Q) /CSM/SS/SSK1-C/SSK152Q.Seq.d/                        799   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL395901|AL395901.1 T3 end of clone AR0AA026H03 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    60  3e-05  1
AJ494852|AJ494852.1 Oikopleura dioica partial H2A.4 gene for histone H2A.4.    54  0.002  1
AL418813|AL418813.1 T7 end of clone AX0AA034F12 of library AX0AA from strain CBS 7064 of Pichia farinosa.    54  0.002  1
AL440801|AL440801.1 T7 end of clone BD0AA014H06 of library BD0AA from strain CBS 94 of  Candida tropicalis.    52  0.006  1
CC163947|CC163947.1 ii66b10.g1 WGS-ZmaysF (DH5a methyl filtered) Zea mays genomic clone ii66b10, DNA sequence.    50  0.024  1
CC163946|CC163946.1 ii66b10.b1 WGS-ZmaysF (DH5a methyl filtered) Zea mays genomic clone ii66b10, DNA sequence.    50  0.024  1
BM111490|BM111490.1 EST559026 potato roots Solanum tuberosum cDNA clone cPRO11D7 5' end , mRNA sequence.    38  0.049  2
BG592519|BG592519.1 EST491197 cSTS Solanum tuberosum cDNA clone cSTS1H11 5' sequence, mRNA sequence.    38  0.059  2
BG599151|BG599151.1 EST504051 cSTS Solanum tuberosum cDNA clone cSTS23B13 5' sequence, mRNA sequence.    38  0.062  2
BI920756|BI920756.1 EST540679 potato microtubers, in vitro-grown Solanum tuberosum cDNA clone cSTE26I24 5' end, mRNA sequence.    38  0.062  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q27442) RecName: Full=Histone H2B;  &FB334594_1(FB334594|pid:no...   111   9e-24
(P02284) RecName: Full=Histone H2B, gonadal;                          110   2e-23
AJ238321_1(AJ238321|pid:none) Asellus aquaticus 5S rRNA gene, hi...   109   3e-23
EZ000286_1(EZ000286|pid:none) TSA: Amblyomma americanum Contig39...   109   3e-23
(P30757) RecName: Full=Histone H2B;  &S16084(S16084;A57915)           109   3e-23
AB435638_1(AB435638|pid:none) Oryzias latipes H2B mRNA for histo...   109   3e-23
(P19374) RecName: Full=Histone H2B;  &S11313(S11313) &X53330_2(X...   108   4e-23
(Q8I1N0) RecName: Full=Histone H2B;  &AB073635_3(AB073635|pid:no...   108   7e-23
AF378198_5(AF378198|pid:none) Rhynchosciara americana histone H1...   108   7e-23
(P02283) RecName: Full=Histone H2B;  &(P59781) RecName: Fu &(P59...   108   7e-23

 psg: 0.85  gvh: 0.47  alm: 0.40  top: 0.10  tms: 0.00  mit: 0.39  mip: 0.02
 nuc: 0.36  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: nuclear
	 24.0 %: cytoplasmic
	 24.0 %: extracellular, including cell wall
	 12.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VSJ146 is nuc

>VSJ146F.Seq
AAGAAGAGAAAGAAGAGAAAGTCTGACTACACCTCATTCTCAACCTACATCCACAAGTTA
TTGAAACAAATTACTCCACCAACCAATGCCAAGAGTAATGAAAAAGGTGATAGAAAATTC
ACTATCTCATCCAAAGCCATGTCTGTAATGAACTCCTTTGTCCACGATATCTTTGACAGA
ATCGCCACCGAAGCTTCTGGTTTAGCCAAAAAGAAGAAGAGACAAACTTTACACTCAAGA
GATATTCAAGTTGCCGTTCGTATCATTCTCACTGGTGAGTTAGCTAAACATGCCATCTTA
CAAGGTATGACTGCTGTCAACAAGTACAATCCAACTGAAAGCAAAAACTAAATTGGTTCC
AAATGAACTATATTCCTTAAAAAAATATAGTTTCCAATTTTCTTTTTTTTTTGNACTCTC
TCTATCATTCTTTTTTAAAAAAAAAAAANTCAAAAAAAAAA----------