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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSJ108
Library VS
(Link to library)
Clone ID VSJ108
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID VSJ108P
(Link to Original site)
Representative DNA sequence 
>VSJ108 (VSJ108Q) /CSM/VS/VSJ1-A/VSJ108Q.Seq.d/
GACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTT
TAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAA
GAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAA
TTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAG
CCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTC
CAATCACTTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACA
TATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTT
GTCTCTCAAGAXXXXXXXXXXAGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTT
AGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTT
ATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAAGGTG
CCAACGGTGGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGGTGACAT
TACCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTAT
CGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTAC
ATCACTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGT
CATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGT
TACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGT
TACTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGG
AAATTTCTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGG
TAATAAACAAGTTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATA
TTTAAGNATAGAAATAAAACTAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
tyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwyhnpn
frf*RCSILWCHYHWYPRSSLQSSFRYWFIQLVDSIKEMSNHLLLHVIYITNITAVPQAH
MSPTELISPSNTVVVLCQVLSLK---

---GIAFDFAKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPRCQRWV
NSHSVQSITPNTLGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIA
GPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFG
KTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylxie
ikl
Translated Amino Acid sequence (All Frames) 
Frame A:
dlhkrk*nylf*lyf*lllf*lkl*qyh*tsiklqenleeefhkngqtdyllsmlvpqsq
fqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvqslvvacdlhnkynsgasst
yvangtdftiqygsgamsgfvsq---

---rycfrfrqirwyfrscfpihlc*fnstsllqhvitrfsfintllllviknskvptvg
elsfgsidntkytg*hylrpinqrnllgiryg*lcyrwsiswflwyylsrnlrfryithc
wsng*yycpq*kircchlkw*rcll*l*ryqhltkcyhhrcws*icfnskrirfrsy*vr
kd*mfewiygyrvkhgkfldpw*cfhlcllycirfw**tswfrnchsrlnffn*fifkxr
nkt

Frame B:
tyikeneityfnfifsyycfsssfnstiklpssfkri*kksstkmvkqiicsqcwyhnpn
frf*RCSILWCHYHWYPRSSLQSSFRYWFIQLVDSIKEMSNHLLLHVIYITNITAVPQAH
MSPTELISPSNTVVVLCQVLSLK---

---GIAFDFAKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFSFWLSRTPRCQRWV
NSHSVQSITPNTLGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTCHAICDSGTSLIA
GPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLTPKEYVLEVTEFG
KTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG*iflinlylxie
ikl

Frame C:
lt*kkmklliltlflativlaqaltvplnfhqasresrrrvpqkwsnrlsalnagttipi
sdfedaqyygaitigtpgqafkvvfdtgssnlwipskkcpitcccm*ft*qi*qrclkhi
crqrn*fhhpir*wcyvrfclsr---

---vllsispnsmvf*vllsnpslliqfhqsfttcyhkv*fhqhsspsgyqelqgangg*
tlirfnr*hqihwvtlptsh*ptkpignslwmtllsmvnqlvsvvllvtqfaiqvhhsll
vqwlillpsmkn*vlss*mvkvsslivalstpyqmlpspllvvnlf*lqkntf*kllsse
rlnv*vdlwvss*tweisgslvmfsslltilysilvinklvsqlpfkvkff*liyi*x*k
*n*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ108 (VSJ108Q) /CSM/VS/VSJ1-A/VSJ108Q.Seq.d/                       2198   0.0
VFH655 (VFH655Q) /CSM/VF/VFH6-C/VFH655Q.Seq.d/                       1354   0.0
VFI636 (VFI636Q) /CSM/VF/VFI6-B/VFI636Q.Seq.d/                       1350   0.0
AFA888 (AFA888Q) /CSM/AF/AFA8-D/AFA888Q.Seq.d/                       1346   0.0
VFL614 (VFL614Q) /CSM/VF/VFL6-A/VFL614Q.Seq.d/                       1342   0.0
VFN222 (VFN222Q) /CSM/VF/VFN2-A/VFN222Q.Seq.d/                       1336   0.0
VFM309 (VFM309Q) /CSM/VF/VFM3-A/VFM309Q.Seq.d/                       1336   0.0
VFL385 (VFL385Q) /CSM/VF/VFL3-D/VFL385Q.Seq.d/                       1336   0.0
VFK659 (VFK659Q) /CSM/VF/VFK6-C/VFK659Q.Seq.d/                       1336   0.0
VFG570 (VFG570Q) /CSM/VF/VFG5-C/VFG570Q.Seq.d/                       1336   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.   930  0.0  8
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.   930  0.0  9
AL765461|AL765461.1 Arabidopsis thaliana T-DNA flanking sequence GK-139E08-012875.    62  2e-05  1
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62  2e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62  2e-05  1
AF372974|AF372974.1 Arabidopsis thaliana AT4g04460/T26N6_7 mRNA, complete cds.    62  2e-05  1
BE525959|BE525959.1 M64C07STM Arabidopsis developing seed Arabidopsis thaliana cDNA clone 600034526R1 5', mRNA sequence.    62  2e-05  1
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62  2e-05  1
CB814835|CB814835.1 USDA-FP_100247 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-04_A10 5', mRNA sequence.    36  4e-05  3
AB078420|AB078420.1 Brugia malayi asp-2 mRNA for aspartic protease BmAsp-2, complete cds.    40  6e-05  4
dna update 2003. 7.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   207   1e-79
DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   201   2e-78
FJ168036_1(FJ168036|pid:none) Fasciola hepatica cathepsin D-like...   206   5e-78
EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   201   1e-77
EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   204   2e-77
EF000001_1(EF000001|pid:none) Fasciola hepatica cathepsin D-like...   200   9e-76
U90750_1(U90750|pid:none) Schistosoma japonicum aspartic proteas...   189   7e-72
L41346_1(L41346|pid:none) Schistosoma japonicum aspartic protein...   189   7e-72
AB218595_1(AB218595|pid:none) Haemaphysalis longicornis AP mRNA ...   185   7e-72
U60995_1(U60995|pid:none) Schistosoma mansoni aspartic proteinas...   189   2e-71
protein update 2009. 3.25
PSORT 

 psg: 0.84  gvh: 0.64  alm: 0.32  top: 0.60  tms: 0.00  mit: 0.46  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 36.0 %: nuclear
	 16.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VSJ108 is mit
5' end seq. ID VSJ108F
5' end seq. 
>VSJ108F.Seq
GACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTT
TAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAA
GAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAA
TTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAG
CCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTC
CAATCACTTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACA
TATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTT
GTCTCTCAAGA----------
Length of 5' end seq. 431
3' end seq. ID VSJ108Z
3' end seq. 
>VSJ108Z.Seq
----------AGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTT
TCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTT
TAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAAGGTGCCAACGGTGGG
TGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGGTGACATTACCTACGTCC
CATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTATCGATGGTCAAT
CAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTACATCACTCATTG
CTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATG
GTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGTTACCATCACCG
TTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCG
GAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGGAAATTTCTGGA
TCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGGTAATAAACAAG
TTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATATTTAAGNATAG
AAATAAAACTAA
Length of 3' end seq. 722
Connected seq. ID VSJ108P
Connected seq. 
>VSJ108P.Seq
GACTTACATAAAAGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTT
TAGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAA
GAGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAA
TTTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAG
CCTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTC
CAATCACTTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACA
TATGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTT
GTCTCTCAAGA----------AGGTATTGCTTTCGATTTCGCCAAATTCGATGGTATTTT
AGGTCTTGCTTTCCAATCCATCTCTGTTAATTCAATTCCACCAGTCTTTTACAACATGTT
ATCACAAGGTTTAGTTTCATCAACACTCTTCTCCTTCTGGTTATCAAGAACTCCAAGGTG
CCAACGGTGGGTGAACTCTCATTCGGTTCAATCGATAACACCAAATACACTGGGTGACAT
TACCTACGTCCCATTAACCAACGAAACCTATTGGGAATTCGTTATGGATGACTTTGCTAT
CGATGGTCAATCAGCTGGTTTCTGTGGTACTACTTGTCACGCAATTTGCGATTCAGGTAC
ATCACTCATTGCTGGTCCAATGGCTGATATTACTGCCCTCAATGAAAAATTAGGTGCTGT
CATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTAGCGTTATCAACACCTTACCAAATGT
TACCATCACCGTTGCTGGTCGTGAATTTGTTTTAACTCCAAAAGAATACGTTTTAGAAGT
TACTGAGTTCGGAAAGACTGAATGTTTGAGTGGATTTATGGGTATCGAGTTAAACATGGG
AAATTTCTGGATCCTTGGTGATGTTTTCATCTCTGCTTACTATACTGTATTCGATTTTGG
TAATAAACAAGTTGGTTTCGCAACTGCCATTCAAGGTTAAATTTTTTTAATTAATTTATA
TTTAAGNATAGAAATAAAACTAA
Length of connected seq. 1153
Full length Seq ID -
Full length Seq. -
Length of full length seq. -