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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI729
Library VS
(Link to library)
Clone ID VSI729
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16504-1
Original site URL
Representative seq. ID VSI729E
(Link to Original site)
Representative DNA sequence 
>VSI729 (VSI729Q) /CSM/VS/VSI7-B/VSI729Q.Seq.d/
GGATATTCCAGTATGGATTACACCATGTTCAGCATTTATGAGATACATTAAATCGTATCC
AATTGTTGGAACTGTAACAGAGACAATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTT
ATCAACTAGTGAAACCAAAGCATCAGTTTCAGTCGGATTCTTTGGTTGTGAATCATCGAT
GGAAGTTACAAGTGGTTATGAATATGAAGACACTGTTACATCTGAAGAAACTAGAAGTTG
GAGTCAAACTTTAAATGAAGGTTCTTATATTGTTTATCAAAACGTTTTAGTTTATGCTTA
TATAATCTATGGTGGTAGTTATAAACCATACATTGATCAAATGAATAAATTTAATCCTGG
ATTAAATATCAAATTCTTTAGAGAAGATAAATGTATCTTTTTTTGTACCAATTAATCGTG
ATGATGCCTTCACTTCTTCGTTATCAAGATAATACATGGGATCCAGTTGAATATGATGTT
TTAATTGATTACTTGGGTCAAAATCAAGATAAATGGTTTTCTGGTGGTTTACCATGAAAA
TAATAATAATAATAATAATAATAATTATATAAATTACATAAAGGTTTCACTATTGGTGTT
TTAATCCTGGTATAAATAAAATTCTAAAAATAAAAAATAAAAAACT
sequence update 2001. 3.26
Translated Amino Acid sequence 
DIPVWITPCSAFMRYIKSYPIVGTVTETMEKKKGFTNRFLSTSETKASVSVGFFGCESSM
EVTSGYEYEDTVTSEETRSWSQTLNEGSYIVYQNVLVYAYIIYGGSYKPYIDQMNKFNPG
LNIKFFREDKCIFFCTN*s**clhffvikiihgiqlnmmf*litwvkikingflvvyhen
nnnnnnnnyinyikvsllvf*swyk*nsknkk*kt
Translated Amino Acid sequence (All Frames) 
Frame A:
gyssmdytmfsiyeih*ivsncwncnrdngkekrfhq*ilin**nqsisfsrilwl*iid
gsykwl*i*rhcyi*rn*klesnfk*rflyclskrfslclynlww*l*tih*sne*i*sw
ikyqil*rr*mylflyqlivmmpsllryqdntwdpveydvlidylgqnqdkwfsgglp*k
********lyklhkgftigvlilv*ikf*k*kikn


Frame B:
DIPVWITPCSAFMRYIKSYPIVGTVTETMEKKKGFTNRFLSTSETKASVSVGFFGCESSM
EVTSGYEYEDTVTSEETRSWSQTLNEGSYIVYQNVLVYAYIIYGGSYKPYIDQMNKFNPG
LNIKFFREDKCIFFCTN*s**clhffvikiihgiqlnmmf*litwvkikingflvvyhen
nnnnnnnnyinyikvsllvf*swyk*nsknkk*kt


Frame C:
ifqyglhhvqhl*dtlnriqllel*qrqwkrkkvspidsyqlvkpkhqfqsdslvvnhrw
klqvvmnmktllhlkklevgvkl*mkvlilfiktf*fmli*smvvvinhtlik*inlild
*isnslekinvsffvpinrddaftsslsr*ymgss*i*cfn*llgsksr*mvfwwftmki
iiiiiiiii*it*rfhywcfnpginkilkiknkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI729 (VSI729Q) /CSM/VS/VSI7-B/VSI729Q.Seq.d/                       1025   0.0
VSJ730 (VSJ730Q) /CSM/VS/VSJ7-B/VSJ730Q.Seq.d/                        999   0.0
VSD543 (VSD543Q) /CSM/VS/VSD5-B/VSD543Q.Seq.d/                        999   0.0
VHJ463 (VHJ463Q) /CSM/VH/VHJ4-C/VHJ463Q.Seq.d/                        999   0.0
VHB403 (VHB403Q) /CSM/VH/VHB4-A/VHB403Q.Seq.d/                        999   0.0
VFI758 (VFI758Q) /CSM/VF/VFI7-C/VFI758Q.Seq.d/                        999   0.0
VFI142 (VFI142Q) /CSM/VF/VFI1-B/VFI142Q.Seq.d/                        999   0.0
VFC716 (VFC716Q) /CSM/VF/VFC7-A/VFC716Q.Seq.d/                        999   0.0
VFJ763 (VFJ763Q) /CSM/VF/VFJ7-C/VFJ763Q.Seq.d/                        993   0.0
VSI168 (VSI168Q) /CSM/VS/VSI1-C/VSI168Q.Seq.d/                        991   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U48706|U48706.1 Dictyostelium discoideum small aggregate formation (smlA) gene, complete cds.   787  0.0  4
X94341|X94341.1 D.discoideum mRNA for unknown protein with homology to spore germination protein.    52  1e-34  6
AC116158|AC116158.5 Homo sapiens chromosome , clone RP11-102L12, complete sequence.    44  0.30  4
CA930567|CA930567.1 MTU4CA.P24.B01 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    34  0.34  2
CA930485|CA930485.1 MTU4CA.P23.B04 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    34  0.34  2
CA930468|CA930468.1 MTU4CA.P22.H11 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    34  0.34  2
CA930577|CA930577.1 MTU4CA.P24.B11 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    34  0.34  2
AC123872|AC123872.4 Mus musculus chromosome 2 clone RP23-254P8, complete sequence.    46  0.53  1
AC134622|AC134622.1 Mus musculus chromosome UNK clone RP23-13I7, WORKING DRAFT SEQUENCE, 8 unordered pieces.    38  0.72  5
AC110380|AC110380.4 Mus musculus clone RP24-194P24, complete sequence.    38  1.1  3
dna update 2003. 7.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000712_1048(CP000712|pid:none) Pseudomonas putida F1, complete...    41   0.032
AC116330_42(AC116330|pid:none) Dictyostelium discoideum chromoso...    38   0.21
AC116330_41(AC116330|pid:none) Dictyostelium discoideum chromoso...    32   1.5
CP000932_787(CP000932|pid:none) Campylobacter lari RM2100, compl...    35   2.3
(Q32LI3) RecName: Full=Uncharacterized protein C12orf71 homolog;       33   5.1
BC109559_1(BC109559|pid:none) Bos taurus hypothetical protein LO...    33   5.1
CP000939_2199(CP000939|pid:none) Clostridium botulinum B1 str. O...    33   8.7
AM412317_2201(AM412317|pid:none) Clostridium botulinum A str. AT...    33   8.7
protein update 2009. 3.24
PSORT 

 psg: 0.75  gvh: 0.44  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VSI729 is cyt
5' end seq. ID VSI729F
5' end seq. 
>VSI729F.Seq
GGATATTCCAGTATGGATTACACCATGTTCAGCATTTATGAGATACATTAAATCGTATCC
AATTGTTGGAACTGTAACAGAGACAATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTT
ATCAACTAGTGAAACCAAAGCATCAGTTTCAGTCGGATTCTTTGGTTGTGAATCATCGAT
GGAAGTTACAAGTGGTTATGAATATGAAGACACTGTTACATCTGAAGAAACTAGAAGTTG
GAGTCAAACTTTAAATGAAGGTTCTTATATTGTTTATCAAAACGTTTTAGTTTATGCTTA
TATAATCTATGGTGGTAGTTATAAACCATACATTGATCAAATGAATAAATTTAATCCTGG
ATTAAATATCAAATTCTTTAGAGAAGATAAATGTATCTTTTTTTGTACCAATTAATCGTG
ATGATGCCTTCACTTCTTCGTTATCAAGATAATACATGGGATCCAGTTGAATATGATGTT
TTAATTGATTACTTGGGTCAAAATCAAGAT----------
Length of 5' end seq. 510
3' end seq. ID VSI729Z
3' end seq. 
>VSI729Z.Seq
----------TATTCCAGTATGGATTACACCATGTTCAGCATTTATGAGATACATTAAAT
CGTATCCAATTGTTGGAACTGTAACAGAGACAATGGAAAAGAAAAAAGGTTTCACCAATA
GATTCTTATCAACTAGTGAAACCAAAGCATCAGTTTCAGTCGGATTCTTTGGTTGTGAAT
CATCGATGGAAGTTACAAGTGGTTATGAATATGAAGACACTGTTACATNTGAAGAAACTA
GAAGTTGGAGTCAAACTTTAAATGAAGGTTCTTATATTGTTTATCAAAACGTTTTAGTTT
ATGCTTATATAATCTATGGTGGTAGTTATAAACCATACATTGATCAAATGAATAAATTTA
ATCCTGGATTAAATATCAAATTCTTTAGAGAAGATAAATGTATCTTTTTTGTACCAATTA
ATCGTGATGATGCCTTCACTCTTCGTTATCAAGATAATACATGGGATCCAGTTGAATATG
ATGTTTTAATTGATTACTTGGGTCAAAATCAAGATAAATGGTTTTCTGGTGGTTTACCAT
GAAAATAATAATAATAATAATAATAATAATTATATAAATTACATAAAGGTTTCACTATTG
GTGTTTTAATCCTGGTATAAATAAAATTCTAAAAATAAAAAATAAAAAACT
Length of 3' end seq. 641
Connected seq. ID VSI729P
Connected seq. 
>VSI729P.Seq
GGATATTCCAGTATGGATTACACCATGTTCAGCATTTATGAGATACATTAAATCGTATCC
AATTGTTGGAACTGTAACAGAGACAATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTT
ATCAACTAGTGAAACCAAAGCATCAGTTTCAGTCGGATTCTTTGGTTGTGAATCATCGAT
GGAAGTTACAAGTGGTTATGAATATGAAGACACTGTTACATCTGAAGAAACTAGAAGTTG
GAGTCAAACTTTAAATGAAGGTTCTTATATTGTTTATCAAAACGTTTTAGTTTATGCTTA
TATAATCTATGGTGGTAGTTATAAACCATACATTGATCAAATGAATAAATTTAATCCTGG
ATTAAATATCAAATTCTTTAGAGAAGATAAATGTATCTTTTTTTGTACCAATTAATCGTG
ATGATGCCTTCACTTCTTCGTTATCAAGATAATACATGGGATCCAGTTGAATATGATGTT
TTAATTGATTACTTGGGTCAAAATCAAGAT----------TATTCCAGTATGGATTACAC
CATGTTCAGCATTTATGAGATACATTAAATCGTATCCAATTGTTGGAACTGTAACAGAGA
CAATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTTATCAACTAGTGAAACCAAAGCAT
CAGTTTCAGTCGGATTCTTTGGTTGTGAATCATCGATGGAAGTTACAAGTGGTTATGAAT
ATGAAGACACTGTTACATNTGAAGAAACTAGAAGTTGGAGTCAAACTTTAAATGAAGGTT
CTTATATTGTTTATCAAAACGTTTTAGTTTATGCTTATATAATCTATGGTGGTAGTTATA
AACCATACATTGATCAAATGAATAAATTTAATCCTGGATTAAATATCAAATTCTTTAGAG
AAGATAAATGTATCTTTTTTGTACCAATTAATCGTGATGATGCCTTCACTCTTCGTTATC
AAGATAATACATGGGATCCAGTTGAATATGATGTTTTAATTGATTACTTGGGTCAAAATC
AAGATAAATGGTTTTCTGGTGGTTTACCATGAAAATAATAATAATAATAATAATAATAAT
TATATAAATTACATAAAGGTTTCACTATTGGTGTTTTAATCCTGGTATAAATAAAATTCT
AAAAATAAAAAATAAAAAACT
Length of connected seq. 1151
Full length Seq ID VSI729E
Full length Seq. 
>VSI729E.Seq
GGATATTCCAGTATGGATTACACCATGTTCAGCATTTATGAGATACATTAAATCGTATCC
AATTGTTGGAACTGTAACAGAGACAATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTT
ATCAACTAGTGAAACCAAAGCATCAGTTTCAGTCGGATTCTTTGGTTGTGAATCATCGAT
GGAAGTTACAAGTGGTTATGAATATGAAGACACTGTTACATCTGAAGAAACTAGAAGTTG
GAGTCAAACTTTAAATGAAGGTTCTTATATTGTTTATCAAAACGTTTTAGTTTATGCTTA
TATAATCTATGGTGGTAGTTATAAACCATACATTGATCAAATGAATAAATTTAATCCTGG
ATTAAATATCAAATTCTTTAGAGAAGATAAATGTATCTTTTTTTGTACCAATTAATCGTG
ATGATGCCTTCACTTCTTCGTTATCAAGATAATACATGGGATCCAGTTGAATATGATGTT
TTAATTGATTACTTGGGTCAAAATCAAGATAAATGGTTTTCTGGTGGTTTACCATGAAAA
TAATAATAATAATAATAATAATAATTATATAAATTACATAAAGGTTTCACTATTGGTGTT
TTAATCCTGGTATAAATAAAATTCTAAAAATAAAAAATAAAAAACT
Length of full length seq. 646