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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI710
Library VS
(Link to library)
Clone ID VSI710
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15502-1
Original site URL
Representative seq. ID VSI710E
(Link to Original site)
Representative DNA sequence 
>VSI710 (VSI710Q) /CSM/VS/VSI7-A/VSI710Q.Seq.d/
CCCGCGTCCGATATATTAAATAATTAAATAAACAAATAAATAAATAAATAAATAAATAAA
ATGACAGGAACTAGAACTTTTTTTGTTGGTGGAAATCATAAAATGAATGGTAGTAAATCA
ATGTTAGAATCTTTAAGTAAAGGAATGAATGAATCAGTTGAAAATAAAGAAAATGTTGAT
ATTTTCATTGCACCATCATATCCATATTTAGAATTTTTATCAAATAAATTAGATAATAAA
AAGTTTAAAGTTTGTTCACAAAATTGTTATAGTGTTGCCAAAGGTGCATTCACAGGTGAA
ATCAGTGCTAATATGTTGGTTGATTTAGGTATTCCATACGTTAATCATTGGTCATAGTGA
GAGAAGAAATGTATTTAGCGAATCAAGTGAGTTAATTACAAAGAAAACAAAGTATGCAAT
TTCATTAGGTCTCACAGTTATCTTATGTTTAGGTGAACTTTTAGCAGACAGAAAATCAAA
TAACACTGAACACATCCCTAAGTGAACAATTGAAAGATTTCGCTTCATTCACTCCAGAAG
AATGGTCAAAGATCGTAATTGCCTATGAACCAGTTTGGGCAATTGGTACTGGTGCCGTTG
CAACTCCACAAGAAGCTCAAGATACTCATGTTTTCATTCGTAAATGGATTAGTGAAAAGG
TTTCTGAAGATGTTGCAAAGAAAACCTCGATCATGTATGGTGGTTCTGTTAATGCTGACA
ATTGTCATAACCTTTCAAAGCAATCTGATATCGATGGTTTCTTAGTTGGTGGTGCTTCCC
TTGTTGCCAGTGATTTCATTGCCATCATTAAATCTGCTGTTCCAAAGAAATATTGAATCT
CAAATTTATAAAAATAAAAATAAAAAATAAATAAAAATCTATAAG
sequence update 2001. 3.26
Translated Amino Acid sequence 
rvryik*lnkqink*ink*ndrn*nffcwwks*new**invrifk*rne*is*k*rkc*y
fhctiisifrifik*ir**kv*slftkll*ccqrcihr*nqc*yvg*frysir*slvivr
eemylanqvs*lqrkqsmqfh*vsqlsyv*vnf*QTENQITLNTSLSEQLKDFASFTPEE
WSKIVIAYEPVWAIGTGAVATPQEAQDTHVFIRKWISEKVSEDVAKKTSIMYGGSVNADN
CHNLSKQSDIDGFLVGGASLVASDFIAIIKSAVPKKY*isnl*k*k*kinknl*
Translated Amino Acid sequence (All Frames) 
Frame A:
pasdilnn*ink*ink*inkmtgtrtffvggnhkmngsksmleslskgmnesvenkenvd
ifiapsypyleflsnkldnkkfkvcsqncysvakgaftgeisanmlvdlgipyvnhws**
ekkci*rik*vnykenkvcnfirshsylmfr*tfsrqkik*h*thp*vnn*kislhslqk
ngqrs*lpmnqfgqlvlvplqlhkklkilmfsfvnglvkrflkmlqrkprscmvvllmlt
ivitfqsnlismvs*lvvlpllpvislpslnllfqrniesqiyknknkk*ikiyk


Frame B:
prpiy*iik*tnk*ink*ik*qelelfllveiik*mvvnqc*nl*vke*mnqlkikkmli
fslhhhihi*nfyqin*iikslkfvhkivivlpkvhsqvksvlicwli*vfhtliighse
rrnvfsesselitkktkyaislgltvilclgelladrksnntehipk*tierfrfihsrr
mvkdrncl*tslgnwywcrcnstrssryscfhs*md**kgf*rcckenldhvwwfc*c*q
ls*pfkai*yrwflswwcfpccq*fhchh*iccskeilnlkfikikiknk*ksi


Frame C:
rvryik*lnkqink*ink*ndrn*nffcwwks*new**invrifk*rne*is*k*rkc*y
fhctiisifrifik*ir**kv*slftkll*ccqrcihr*nqc*yvg*frysir*slvivr
eemylanqvs*lqrkqsmqfh*vsqlsyv*vnf*QTENQITLNTSLSEQLKDFASFTPEE
WSKIVIAYEPVWAIGTGAVATPQEAQDTHVFIRKWISEKVSEDVAKKTSIMYGGSVNADN
CHNLSKQSDIDGFLVGGASLVASDFIAIIKSAVPKKY*isnl*k*k*kinknl*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI710 (VSI710Q) /CSM/VS/VSI7-A/VSI710Q.Seq.d/                       1515   0.0
SSK644 (SSK644Q) /CSM/SS/SSK6-B/SSK644Q.Seq.d/                       1483   0.0
VSD388 (VSD388Q) /CSM/VS/VSD3-D/VSD388Q.Seq.d/                       1463   0.0
VSG406 (VSG406Q) /CSM/VS/VSG4-A/VSG406Q.Seq.d/                        848   0.0
SSM191 (SSM191Q) /CSM/SS/SSM1-D/SSM191Q.Seq.d/                        720   0.0
SSI865 (SSI865Q) /CSM/SS/SSI8-C/SSI865Q.Seq.d/                        537   e-152
SSL279 (SSL279Q) /CSM/SS/SSL2-D/SSL279Q.Seq.d/                        515   e-145
VSI633 (VSI633Q) /CSM/VS/VSI6-B/VSI633Q.Seq.d/                        424   e-117
VSA293 (VSA293Q) /CSM/VS/VSA2-D/VSA293Q.Seq.d/                         38   0.22
VSA207 (VSA207Q) /CSM/VS/VSA2-A/VSA207Q.Seq.d/                         38   0.22
own update 2001. 3.31
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   696  0.0  5
AF124845|AF124845.1 Candida albicans triose phosphate isomerase (TPI1) gene, complete cds.    46  1e-07  3
AI508110|AI508110.1 BSBmL3SZ08K6SK Brugia malayi infective larva cDNA (SAW94WL-BmL3) Brugia malayi cDNA clone BSBmL3SZ08K6SK 5', mRNA sequence.    46  4e-07  3
E17071|E17071.1 Candida albicans mRNA for antigen peptide.        46  5e-07  2
E24635|E24635.1 Antigen protein and nucleic acid encoding the same.    46  5e-07  2
AJ390491|AJ390491.1 Candida albicans mRNA for triosephosphate isomerase (tpi1 gene).    46  5e-07  2
AW561772|AW561772.1 KJBmL3SZ16F17SK Brugia malayi infective larva cDNA (SAW94WL-BmL3) Brugia malayi cDNA clone KJBmL3SZ16F17SK 5', mRNA sequence.    46  6e-07  3
AI723732|AI723732.1 BSBmL3SZ45O17SK Brugia malayi infective larva cDNA (SAW94WL-BmL3) Brugia malayi cDNA clone BSBmL3SZ45O17SK 5', mRNA sequence.    46  6e-07  3
AI723702|AI723702.1 BSBmL3SZ45I8SK Brugia malayi infective larva cDNA (SAW94WL-BmL3) Brugia malayi cDNA clone BSBmL3SZ45I8SK 5', mRNA sequence.    44  2e-06  3
AF063107|AF063107.1 Achlya bisexualis triosephosphate isomerase + glyceraldehyde-3-phosphate dehydrogenase (TigA) mRNA, complete cds.    50  2e-05  2
dna update 2003. 7.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869R8) RecName: Full=Triosephosphate isomerase;          Short...   226   e-133
CP000601_176(CP000601|pid:none) Ostreococcus lucimarinus CCE9901...   124   3e-56
(P48501) RecName: Full=Triosephosphate isomerase;          Short...   114   7e-56
CR954209_8(CR954209|pid:none) Ostreococcus tauri strain OTTH0595...   126   2e-55
AX172665_1(AX172665|pid:none) Sequence 155 from Patent WO0144476.     132   3e-55
EF147662_1(EF147662|pid:none) Populus trichocarpa clone WS0124_D...   134   8e-55
EU976195_1(EU976195|pid:none) Zea mays clone 687466 triosephosph...   135   8e-55
(Q9M4S8) RecName: Full=Triosephosphate isomerase, chloroplastic;...   131   1e-53
AY438596_1(AY438596|pid:none) Solanum chacoense triose phosphate...   120   1e-53
(P48497) RecName: Full=Triosephosphate isomerase, cytosolic;    ...   132   2e-53
protein update 2009. 3.24
PSORT 

 psg: 0.75  gvh: 0.47  alm: 0.28  top: 0.77  tms: 0.07  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	 12.0 %: vesicles of secretory system
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VSI710 is cyt
5' end seq. ID VSI710F
5' end seq. 
>VSI710F.Seq
CCCGCGTCCGATATATTAAATAATTAAATAAACAAATAAATAAATAAATAAATAAATAAA
ATGACAGGAACTAGAACTTTTTTTGTTGGTGGAAATCATAAAATGAATGGTAGTAAATCA
ATGTTAGAATCTTTAAGTAAAGGAATGAATGAATCAGTTGAAAATAAAGAAAATGTTGAT
ATTTTCATTGCACCATCATATCCATATTTAGAATTTTTATCAAATAAATTAGATAATAAA
AAGTTTAAAGTTTGTTCACAAAATTGTTATAGTGTTGCCAAAGGTGCATTCACAGGTGAA
ATCAGTGCTAATATGTTGGTTGATTTAGGTATTCCATACGTTATCATTGGTCATAGTGAG
AGAAGAAATGTATTTAGCGAATCAAGTGAGTTAATTACAAAGAAAACAAAGTATGCAATT
TCATTAGGTCTCACAGTTA----------
Length of 5' end seq. 439
3' end seq. ID VSI710Z
3' end seq. 
>VSI710Z.Seq
----------TATAGTGTTGCCAAAGGTGCATTCACAGGTGAAATCAGTGCTAATATGTT
GGTTGATTTAGGTATTCCATACGTTAATCATTGGTCATAGTGAGAGAAGAAATGTATTTA
GCGAATCAAGTGAGTTAATTACAAAGAAAACAAAGTATGCAATTTCATTAGGTCTCACAG
TTATCTTATGTTTAGGTGAACTTTTAGCAGACAGAAAATCAAATAACACTGAACACATCC
CTAAGTGAACAATTGAAAGATTTCGCTTCATTCACTCCAGAAGAATGGTCAAAGATCGTA
ATTGCCTATGAACCAGTTTGGGCAATTGGTACTGGTGCCGTTGCAACTCCACAAGAAGCT
CAAGATACTCATGTTTTCATTCGTAAATGGATTAGTGAAAAGGTTTCTGAAGATGTTGCA
AAGAAAACCTCGATCATGTATGGTGGTTCTGTTAATGCTGACAATTGTCATAACCTTTCA
AAGCAATCTGATATCGATGGTTTCTTAGTTGGTGGTGCTTCCCTTGTTGCCAGTGATTTC
ATTGCCATCATTAAATCTGCTGTTCCAAAGAAATATTGAATCTCAAATTTATAAAAATAA
AAATAAAAAATAAATAAAAATCTATAAG
Length of 3' end seq. 618
Connected seq. ID VSI710P
Connected seq. 
>VSI710P.Seq
CCCGCGTCCGATATATTAAATAATTAAATAAACAAATAAATAAATAAATAAATAAATAAA
ATGACAGGAACTAGAACTTTTTTTGTTGGTGGAAATCATAAAATGAATGGTAGTAAATCA
ATGTTAGAATCTTTAAGTAAAGGAATGAATGAATCAGTTGAAAATAAAGAAAATGTTGAT
ATTTTCATTGCACCATCATATCCATATTTAGAATTTTTATCAAATAAATTAGATAATAAA
AAGTTTAAAGTTTGTTCACAAAATTGTTATAGTGTTGCCAAAGGTGCATTCACAGGTGAA
ATCAGTGCTAATATGTTGGTTGATTTAGGTATTCCATACGTTATCATTGGTCATAGTGAG
AGAAGAAATGTATTTAGCGAATCAAGTGAGTTAATTACAAAGAAAACAAAGTATGCAATT
TCATTAGGTCTCACAGTTA----------TATAGTGTTGCCAAAGGTGCATTCACAGGTG
AAATCAGTGCTAATATGTTGGTTGATTTAGGTATTCCATACGTTAATCATTGGTCATAGT
GAGAGAAGAAATGTATTTAGCGAATCAAGTGAGTTAATTACAAAGAAAACAAAGTATGCA
ATTTCATTAGGTCTCACAGTTATCTTATGTTTAGGTGAACTTTTAGCAGACAGAAAATCA
AATAACACTGAACACATCCCTAAGTGAACAATTGAAAGATTTCGCTTCATTCACTCCAGA
AGAATGGTCAAAGATCGTAATTGCCTATGAACCAGTTTGGGCAATTGGTACTGGTGCCGT
TGCAACTCCACAAGAAGCTCAAGATACTCATGTTTTCATTCGTAAATGGATTAGTGAAAA
GGTTTCTGAAGATGTTGCAAAGAAAACCTCGATCATGTATGGTGGTTCTGTTAATGCTGA
CAATTGTCATAACCTTTCAAAGCAATCTGATATCGATGGTTTCTTAGTTGGTGGTGCTTC
CCTTGTTGCCAGTGATTTCATTGCCATCATTAAATCTGCTGTTCCAAAGAAATATTGAAT
CTCAAATTTATAAAAATAAAAATAAAAAATAAATAAAAATCTATAAG
Length of connected seq. 1057
Full length Seq ID VSI710E
Full length Seq. 
>VSI710E.Seq
CCCGCGTCCGATATATTAAATAATTAAATAAACAAATAAATAAATAAATAAATAAATAAA
ATGACAGGAACTAGAACTTTTTTTGTTGGTGGAAATCATAAAATGAATGGTAGTAAATCA
ATGTTAGAATCTTTAAGTAAAGGAATGAATGAATCAGTTGAAAATAAAGAAAATGTTGAT
ATTTTCATTGCACCATCATATCCATATTTAGAATTTTTATCAAATAAATTAGATAATAAA
AAGTTTAAAGTTTGTTCACAAAATTGTTATAGTGTTGCCAAAGGTGCATTCACAGGTGAA
ATCAGTGCTAATATGTTGGTTGATTTAGGTATTCCATACGTTAATCATTGGTCATAGTGA
GAGAAGAAATGTATTTAGCGAATCAAGTGAGTTAATTACAAAGAAAACAAAGTATGCAAT
TTCATTAGGTCTCACAGTTATCTTATGTTTAGGTGAACTTTTAGCAGACAGAAAATCAAA
TAACACTGAACACATCCCTAAGTGAACAATTGAAAGATTTCGCTTCATTCACTCCAGAAG
AATGGTCAAAGATCGTAATTGCCTATGAACCAGTTTGGGCAATTGGTACTGGTGCCGTTG
CAACTCCACAAGAAGCTCAAGATACTCATGTTTTCATTCGTAAATGGATTAGTGAAAAGG
TTTCTGAAGATGTTGCAAAGAAAACCTCGATCATGTATGGTGGTTCTGTTAATGCTGACA
ATTGTCATAACCTTTCAAAGCAATCTGATATCGATGGTTTCTTAGTTGGTGGTGCTTCCC
TTGTTGCCAGTGATTTCATTGCCATCATTAAATCTGCTGTTCCAAAGAAATATTGAATCT
CAAATTTATAAAAATAAAAATAAAAAATAAATAAAAATCTATAAG
Length of full length seq. 885