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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI646
Library VS
(Link to library)
Clone ID VSI646
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16543-1
Original site URL
Representative seq. ID VSI646P
(Link to Original site)
Representative DNA sequence 
>VSI646 (VSI646Q) /CSM/VS/VSI6-B/VSI646Q.Seq.d/
ATCAAATAAATAATTTATATTAAAAAATGTCCAGAGTAATTCATATTTCATCTAATGAAG
AATTAGATAAACATTTGCAAGCTGAAAGATTAGTGATTGACTTTAGTGCTGCATGGTGTG
GTCCATGTAGAGCAATATCTCCGGTATTTGAAAAGCTTTCAAACGAATTTGTAACATTTA
CATTCGTTCATGTTGACATTGATAAACTAAGTGGTCATCCAATAGTAAAAGAAATTAGAA
GTGTTCCTACATTCTATTTTTACAGAAATGGAGCTAAAGTATCAGAATTCAGTGGTGCCA
ATGAAGCAACTCTAAGAAGTACTCTAGAAGCAAACAAATAATTTTTTGTTAAATTAATAA
TTGTACAAAAAATGCAACAAATTAAATAAATAAAAAAAAAAAAATAATTCNCAAATTNTT
TTNTTTAATTTAAAAAAAAAAXXXXXXXXXXCCACGGGTCCGATCAAATAAATAATTTAT
ATTAAAAAATGTCCANAGTAATTCATATTTCATNTAATGAANAATTANATNAACATTTGC
AAGCTGAAANATTAGTGATTGACTTTAGTGCTGCATGGTGTGGNCCATGTANANCAANAT
NTCCGGNATTTGAAAAGCTTTCAAACGAATTTGNAACATTTACATTCGTTCATGTTGACA
TTGATAAACTAAGTGGTCATCCNATAGTAAAANAAATTANAAGTGTTCCTACNTTCTATT
TTTACANAAATGGAGCTAAAGNATCAGAATTCAGTGGNGCCAATGAAGCAACT
sequence update 2001. 3.22
Translated Amino Acid sequence 
ik*iiyikkcpe*fifhlmkn*inicklkd**ltlvlhgvvhveqylrylksfqtnl*hl
hsfmltlin*vviq**kklevflhsiftemelkyqnsvvpmkql*evl*kqtnnflln**
lykkcnklnk*KKKNNSQIXXFNLKKK---

---HGSDQINNLY*kmsxvihisxnexlxxhlqaexlvidfsaawcgpcxxxxpxfekls
nefxtftfvhvdidklsghpivkxixsvptfyfyxngakxsefsganeat
Translated Amino Acid sequence (All Frames) 
Frame A:
ik*iiyikkcpe*fifhlmkn*inicklkd**ltlvlhgvvhveqylrylksfqtnl*hl
hsfmltlin*vviq**kklevflhsiftemelkyqnsvvpmkql*evl*kqtnnflln**
lykkcnklnk*KKKNNSQIXXFNLKKK---

---prvrsnk*filknvxsnsyfx**xixxtfas*xisd*l*ccmvwxmxxxxsgi*kaf
krixniyirsc*h**tkwssxskxnxkcsyxlflxkws*xiriqwxq*sn

Frame B:
snk*filknvqsnsyfi**rir*tfas*kisd*l*ccmvwsm*snisgi*kafkricniy
irsc*h**tkwssnskrn*kcsyilflqkws*siriqwcq*snskkysrskqiifc*inn
ctknatn*inkkkkiixkxfxli*kk---

---HGSDQINNLY*kmsxvihisxnexlxxhlqaexlvidfsaawcgpcxxxxpxfekls
nefxtftfvhvdidklsghpivkxixsvptfyfyxngakxsefsganeat

Frame C:
qinnly*kmsrvihissneeldkhlqaerlvidfsaawcgpcraispvfeklsnefvtft
fvhvdidklsghpivkeirsvptfyfyrngakvsefsganeatlrstleank*ffvklii
vqkmqqik*ikkkk*fxnxfx*fkkk---

---tgpik*iiyikkcpx*fifhxmxnxxnicklkx**ltlvlhgvxhvxqxxrxlksfq
tnlxhlhsfmltlin*vvix**xklxvflxsiftxmelkxqnsvxpmkq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ416 (VSJ416Q) /CSM/VS/VSJ4-A/VSJ416Q.Seq.d/                        775   0.0
VSI646 (VSI646Q) /CSM/VS/VSI6-B/VSI646Q.Seq.d/                        775   0.0
SSM584 (SSM584Q) /CSM/SS/SSM5-D/SSM584Q.Seq.d/                        775   0.0
SSM341 (SSM341Q) /CSM/SS/SSM3-B/SSM341Q.Seq.d/                        775   0.0
SSE809 (SSE809Q) /CSM/SS/SSE8-A/SSE809Q.Seq.d/                        775   0.0
SSA493 (SSA493Q) /CSM/SS/SSA4-D/SSA493Q.Seq.d/                        775   0.0
AHG261 (AHG261Q) /CSM/AH/AHG2-C/AHG261Q.Seq.d/                        775   0.0
VSD821 (VSD821Q) /CSM/VS/VSD8-A/VSD821Q.Seq.d/                        771   0.0
VSD665 (VSD665Q) /CSM/VS/VSD6-C/VSD665Q.Seq.d/                        771   0.0
VSD645 (VSD645Q) /CSM/VS/VSD6-B/VSD645Q.Seq.d/                        771   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU269880) Dictyostelium discoideum vegetative cDNA clone:VS...   654   0.0    1
(AU269018) Dictyostelium discoideum vegetative cDNA clone:VS...   654   0.0    1
(AU261936) Dictyostelium discoideum vegetative cDNA clone:VS...   654   0.0    1
(AU039068) Dictyostelium discoideum slug cDNA, clone SSM341.      654   0.0    1
(C93126) Dictyostelium discoideum slug cDNA, clone SSM584.        654   0.0    1
(BJ339191) Dictyostelium discoideum cDNA clone:dda64p09, 5' ...   654   0.0    1
(BJ333680) Dictyostelium discoideum cDNA clone:dda43i16, 5' ...   654   0.0    1
(AU264605) Dictyostelium discoideum vegetative cDNA clone:VS...   650   0.0    1
(AU264394) Dictyostelium discoideum vegetative cDNA clone:VS...   650   0.0    1
(AU264360) Dictyostelium discoideum vegetative cDNA clone:VS...   650   0.0    1
dna update 2009. 7.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P29447) RecName: Full=Thioredoxin-3;          Short=Trx-3;           185   1e-45
C46264(C46264) thioredoxin 3 - slime mold (Dictyostelium discoid...   182   1e-44
(P29446) RecName: Full=Thioredoxin-2;          Short=Trx-2; Flag...   182   1e-44
BC129794_1(BC129794|pid:none) Xenopus laevis hypothetical protei...    94   6e-18
BC166957_1(BC166957|pid:none) Xenopus tropicalis hypothetical pr...    92   1e-17
BC072884_1(BC072884|pid:none) Xenopus laevis MGC80314 protein, m...    91   4e-17
(Q9DGI3) RecName: Full=Thioredoxin;          Short=Trx;  &AF2936...    87   6e-16
FJ384985_1(FJ384985|pid:none) Bombina orientalis thioredoxin mRN...    85   2e-15
DQ215312_1(DQ215312|pid:none) Taeniopygia guttata clone 0058P004...    85   3e-15
(P08629) RecName: Full=Thioredoxin;          Short=Trx;  &A30006...    85   3e-15
protein update 2009. 3.24
PSORT 

 psg: 0.77  gvh: 0.24  alm: 0.75  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.27  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 16.0 %: cytoskeletal
	 16.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VSI646 is nuc
5' end seq. ID VSI646F
5' end seq. 
>VSI646F.Seq
ATCAAATAAATAATTTATATTAAAAAATGTCCAGAGTAATTCATATTTCATCTAATGAAG
AATTAGATAAACATTTGCAAGCTGAAAGATTAGTGATTGACTTTAGTGCTGCATGGTGTG
GTCCATGTAGAGCAATATCTCCGGTATTTGAAAAGCTTTCAAACGAATTTGTAACATTTA
CATTCGTTCATGTTGACATTGATAAACTAAGTGGTCATCCAATAGTAAAAGAAATTAGAA
GTGTTCCTACATTCTATTTTTACAGAAATGGAGCTAAAGTATCAGAATTCAGTGGTGCCA
ATGAAGCAACTCTAAGAAGTACTCTAGAAGCAAACAAATAATTTTTTGTTAAATTAATAA
TTGTACAAAAAATGCAACAAATTAAATAAATAAAAAAAAAAAAATAATTCNCAAATTNTT
TTNTTTAATTTAAAAAAAAAA----------
Length of 5' end seq. 441
3' end seq. ID VSI646Z
3' end seq. 
>VSI646Z.Seq
----------CCACGGGTCCGATCAAATAAATAATTTATATTAAAAAATGTCCANAGTAA
TTCATATTTCATNTAATGAANAATTANATNAACATTTGCAAGCTGAAANATTAGTGATTG
ACTTTAGTGCTGCATGGTGTGGNCCATGTANANCAANATNTCCGGNATTTGAAAAGCTTT
CAAACGAATTTGNAACATTTACATTCGTTCATGTTGACATTGATAAACTAAGTGGTCATC
CNATAGTAAAANAAATTANAAGTGTTCCTACNTTCTATTTTTACANAAATGGAGCTAAAG
NATCAGAATTCAGTGGNGCCAATGAAGCAACT
Length of 3' end seq. 322
Connected seq. ID VSI646P
Connected seq. 
>VSI646P.Seq
ATCAAATAAATAATTTATATTAAAAAATGTCCAGAGTAATTCATATTTCATCTAATGAAG
AATTAGATAAACATTTGCAAGCTGAAAGATTAGTGATTGACTTTAGTGCTGCATGGTGTG
GTCCATGTAGAGCAATATCTCCGGTATTTGAAAAGCTTTCAAACGAATTTGTAACATTTA
CATTCGTTCATGTTGACATTGATAAACTAAGTGGTCATCCAATAGTAAAAGAAATTAGAA
GTGTTCCTACATTCTATTTTTACAGAAATGGAGCTAAAGTATCAGAATTCAGTGGTGCCA
ATGAAGCAACTCTAAGAAGTACTCTAGAAGCAAACAAATAATTTTTTGTTAAATTAATAA
TTGTACAAAAAATGCAACAAATTAAATAAATAAAAAAAAAAAAATAATTCNCAAATTNTT
TTNTTTAATTTAAAAAAAAAA----------CCACGGGTCCGATCAAATAAATAATTTAT
ATTAAAAAATGTCCANAGTAATTCATATTTCATNTAATGAANAATTANATNAACATTTGC
AAGCTGAAANATTAGTGATTGACTTTAGTGCTGCATGGTGTGGNCCATGTANANCAANAT
NTCCGGNATTTGAAAAGCTTTCAAACGAATTTGNAACATTTACATTCGTTCATGTTGACA
TTGATAAACTAAGTGGTCATCCNATAGTAAAANAAATTANAAGTGTTCCTACNTTCTATT
TTTACANAAATGGAGCTAAAGNATCAGAATTCAGTGGNGCCAATGAAGCAACT
Length of connected seq. 763
Full length Seq ID -
Full length Seq. -
Length of full length seq. -