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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB848
Library AF
(Link to library)
Clone ID AFB848
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFB848P
(Link to Original site)
Representative DNA sequence 
>AFB848 (AFB848Q) /CSM/AF/AFB8-B/AFB848Q.Seq.d/
AATNTNATATTTATAATNTATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAG
ACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCA
TTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAA
AGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAA
TCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCC
AAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAA
AGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATT
CGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAG
AGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAA
AGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTA
CAAAGTACTCGTCGATAACAAXXXXXXXXXXTCACCAGATAACACCTACAAAGTACTCGT
CGATAACAAAGAGATCCAAGCAGGTANCCTCGCTGATGATTGGGAATTACTTCCATCAAA
ACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGA
TGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCC
AGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAAC
CATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATA
CAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACT
CTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTC
CATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAA
GACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAA
AGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCCGCCGAAAAAGCTGCTGAAAAAGA
AGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACNAG
ACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
xxyl*xiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHK
EDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQK
VDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINK
VETDQLTHQYTLVISPDNTYKVLVDN---

---SPDNTYKVLVDNKEIQAGXLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKP
AGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHP
LIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQE
AEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTRQKRRSQK
INQKS
Translated Amino Acid sequence (All Frames) 
Frame A:
nxifixylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*
rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tk
s*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqq
s*nrsinssihfghftr*hlqstrr*q---

---SPDNTYKVLVDNKEIQAGXLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKP
AGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHP
LIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQE
AEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTRQKRRSQK
INQKS

Frame B:
xxyl*xiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHK
EDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQK
VDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINK
VETDQLTHQYTLVISPDNTYKVLVDN---

---hqitptkyssitkrskqvxslmignyfhqnkskiqnnpnqsiglt*rksmtqkmlnq
pvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth
*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptkk
pkrkclttskppklkrerkltkn*ppkkllkkkpkklmkkkkkslkki*lkxdkkeevkk
stkk

Frame C:
xxiynxsflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigik
kmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkk
livvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstk
lkpin*lintlwsfhqitptkyssit---

---tr*hlqstrr*qrdpsrxpr**lgitsiktnqrsktiqtsrlg*rkgnr*prrc*ts
rs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpi
n**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprs
rkenv*rprsrqs*rekes*rkisrrksc*krsrrs**rrrrsr*rrfs*nxtkkkkskn
qpkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB848 (AFB848Q) /CSM/AF/AFB8-B/AFB848Q.Seq.d/                       2393   0.0
VFA823 (VFA823Q) /CSM/VF/VFA8-A/VFA823Q.Seq.d/                       1213   0.0
AFE410 (AFE410Q) /CSM/AF/AFE4-A/AFE410Q.Seq.d/                       1213   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                       1211   0.0
SFH171 (SFH171Q) /CSM/SF/SFH1-C/SFH171Q.Seq.d/                       1211   0.0
AFO794 (AFO794Q) /CSM/AF/AFO7-D/AFO794Q.Seq.d/                       1211   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1211   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       1211   0.0
AFI574 (AFI574Q) /CSM/AF/AFI5-D/AFI574Q.Seq.d/                       1211   0.0
AFH852 (AFH852Q) /CSM/AF/AFH8-C/AFH852Q.Seq.d/                       1211   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1203  0.0  2
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  4e-28  4
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  1e-21  4
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  8e-11  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74  9e-11  2
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74  5e-09  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74  5e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74  5e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74  5e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74  5e-09  1
dna update 2003. 7.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   668   0.0
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   403   e-111
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   402   e-110
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   402   e-110
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   402   e-110
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   401   e-110
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   401   e-110
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   401   e-110
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   399   e-110
L27348_1(L27348|pid:none) Hordeum vulgare calreticulin (CRH1) mR...   397   e-109
protein update 2009. 3.27
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum

>> prediction for AFB848 is exc
5' end seq. ID AFB848F
5' end seq. 
>AFB848F.Seq
AATNTNATATTTATAATNTATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAG
ACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCA
TTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAA
AGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAA
TCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCC
AAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAA
AGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATT
CGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAG
AGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAA
AGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTA
CAAAGTACTCGTCGATAACAA----------
Length of 5' end seq. 621
3' end seq. ID AFB848Z
3' end seq. 
>AFB848Z.Seq
----------TCACCAGATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCAAGC
AGGTANCCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACA
ATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACC
AGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGA
TTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAA
AGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCC
ATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGG
TGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGT
CACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGA
AGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGC
TGACGAAAAATTAGCCGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGA
AGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACNAGACAAAAAAGAAGAAGTCAAAA
AATCAACCAAAAAAGT
Length of 3' end seq. 726
Connected seq. ID AFB848P
Connected seq. 
>AFB848P.Seq
AATNTNATATTTATAATNTATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAG
ACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCA
TTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAA
AGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAA
TCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCC
AAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAA
AGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATT
CGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAG
AGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAA
AGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTA
CAAAGTACTCGTCGATAACAA----------TCACCAGATAACACCTACAAAGTACTCGT
CGATAACAAAGAGATCCAAGCAGGTANCCTCGCTGATGATTGGGAATTACTTCCATCAAA
ACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGA
TGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCC
AGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAAC
CATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATA
CAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACT
CTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTC
CATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAA
GACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAA
AGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCCGCCGAAAAAGCTGCTGAAAAAGA
AGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACNAG
ACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT
Length of connected seq. 1347
Full length Seq ID -
Full length Seq. -
Length of full length seq. -