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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI548
Library VS
(Link to library)
Clone ID VSI548
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U05732-1
Original site URL
Representative seq. ID VSI548E
(Link to Original site)
Representative DNA sequence 
>VSI548 (VSI548Q) /CSM/VS/VSI5-B/VSI548Q.Seq.d/
GGCAAAAAAACTAATTAGAAGTGAGGATGGTGTAAAATTTTATGATGATGAATTTATTTT
TTACCAAGAAGGTAACATCCCTGTGATTGTAACATTTCCACATAGTGGTAAATTAAAATT
AAGATATTCCAATAAGAAAATCAGGATGTATTGATCCAGATTGGTGGACAGAGGATTTAG
CAATTTGTATGATTCAAGCATTTAATTGTATTTCATGTTGTTGTGGTTGTACTGTAAAAG
ATAAAGATGATAATAAAGCTGAATGTTGTAAACCATCTTCATCATCATCATCGTGTTCTC
CAACAACAACATCTGAAGAATGTTCATGTTGTAATATTAATATAAGTTCAAATAAAGTCG
ATTACAAGTTAGAATGCCCAAGACCATATATTGTATATTCAAAGATTCATAGAGAAATGT
GTGATTTTAATAGAGGTGAATCGAGTGCCTTTGAAGATGAACGTATGAAACCACATTATC
AATTATATCATGATACAATTAAATCATTTATTTCAAAGATTAAATCAGAGAATCCTCAAT
ACTATAATGGTGGTAAAGTTACATTATTGGATATTCACGGTCAATCGGATATACCATCCC
ATATATTACGTGGTACAAAAGATACAAATACAGTTAAACCAATGTTAAAACATTTAGGTA
ATGATTCATTCATTGGTGAGAATAGTATTTTTGGGTACCTTTTAAATGTTGGTGGTATTG
ATGTATTCCCATTAAATAATTTACCAAGTGATCAAACTACAGATCCAACAAATCCAAAAT
ATGTACCACCAAATAAAATACCACATAACAAAAATGAATCTTATAATCCATTAAATCCAT
ACATTGAACATCCAGAATTTAATGGAGCATTCACAGTTATAAATTATTCAAAAAATTATA
CAATCAATTGTATTCAAGTTGAAATTGGTTATTTATGGAGATCAACAATTGAAAACCGTA
TTAAATTTTCACGTACTTTAAGATCTTCAATTTTAAATTTTATTAATAATTATGTTTAAA
ATAAAAAATAATAATAATAAAATAAATAAATAAATAAAACAATG
sequence update 2001. 3.26
Translated Amino Acid sequence 
qkn*levrmv*nfmmmnlfftkkvtsl*l*hfhivvn*n*DIPIRKSGCIDPDWWTEDLA
ICMIQAFNCISCCCGCTVKDKDDNKAECCKPSSSSSSCSPTTTSEECSCCNINISSNKVD
YKLECPRPYIVYSKIHREMCDFNRGESSAFEDERMKPHYQLYHDTIKSFISKIKSENPQY
YNGGKVTLLDIHGQSDIPSHILRGTKDTNTVKPMLKHLGNDSFIGENSIFGYLLNVGGID
VFPLNNLPSDQTTDPTNPKYVPPNKIPHNKNESYNPLNPYIEHPEFNGAFTVINYSKNYT
INCIQVEIGYLWRSTIENRIKFSRTLRSSILNFINNYV*nkk****nk*inktm
Translated Amino Acid sequence (All Frames) 
Frame A:
gkktn*k*gwckil***iyflprr*hpcdcnist*w*ikikifq*enqdvliqiggqri*
qfv*fkhlivfhvvvvvl*kikmiiklnvvnhlhhhhrvlqqqhlknvhvvili*vqiks
its*naqdhilyiqrfiekcvilievnrvplkmnv*nhiinyimiqlnhlfqrlnqriln
timvvklhywiftvnriyhpiyyvvqkiqiqlnqc*ni*vmihslvrivflgtf*mlvvl
mysh*iiyqviklqiqqiqnmyhqikyhitkmnliih*ihtlniqnlmehsql*iiqkii
qsivfklklviygdqqlktvlnfhvl*dlqf*illiimfkiknnnnkink*ikq


Frame B:
akklirsedgvkfyddefifyqegnipvivtfphsgklklrysnkkirmy*srlvdrgfs
nlydssi*lyfmllwlyckr*r***s*ml*tifiiiivfsnnni*rmfml*y*ykfk*sr
lqvrmpktiycifkds*rnv*f**r*iecl*r*tyettlsiis*yn*iiyfkd*iressi
l*ww*syiigysrsigytipyitwykrykys*tnvktfr**fihw*e*yfwvpfkcwwy*
cipik*ftk*snyrsnkskicttk*ntt*qk*il*siksih*tsri*wsihsyklfkkly
nqlyss*nwlfmeinn*kpy*iftyfkifnfkfy**lclk*kiiiik*ink*nn


Frame C:
qkn*levrmv*nfmmmnlfftkkvtsl*l*hfhivvn*n*DIPIRKSGCIDPDWWTEDLA
ICMIQAFNCISCCCGCTVKDKDDNKAECCKPSSSSSSCSPTTTSEECSCCNINISSNKVD
YKLECPRPYIVYSKIHREMCDFNRGESSAFEDERMKPHYQLYHDTIKSFISKIKSENPQY
YNGGKVTLLDIHGQSDIPSHILRGTKDTNTVKPMLKHLGNDSFIGENSIFGYLLNVGGID
VFPLNNLPSDQTTDPTNPKYVPPNKIPHNKNESYNPLNPYIEHPEFNGAFTVINYSKNYT
INCIQVEIGYLWRSTIENRIKFSRTLRSSILNFINNYV*nkk****nk*inktm
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI548 (VSI548Q) /CSM/VS/VSI5-B/VSI548Q.Seq.d/                       1939   0.0
SSL135 (SSL135Q) /CSM/SS/SSL1-B/SSL135Q.Seq.d/                       1852   0.0
SLC101 (SLC101Q) /CSM/SL/SLC1-A/SLC101Q.Seq.d/                         42   0.017
VSI481 (VSI481Q) /CSM/VS/VSI4-D/VSI481Q.Seq.d/                         40   0.068
VSH492 (VSH492Q) /CSM/VS/VSH4-D/VSH492Q.Seq.d/                         40   0.068
VHP752 (VHP752Q) /CSM/VH/VHP7-C/VHP752Q.Seq.d/                         40   0.068
VHP303 (VHP303Q) /CSM/VH/VHP3-A/VHP303Q.Seq.d/                         40   0.068
VHP250 (VHP250Q) /CSM/VH/VHP2-C/VHP250Q.Seq.d/                         40   0.068
VHO730 (VHO730Q) /CSM/VH/VHO7-B/VHO730Q.Seq.d/                         40   0.068
VHO704 (VHO704Q) /CSM/VH/VHO7-A/VHO704Q.Seq.d/                         40   0.068
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116979) Dictyostelium discoideum chromosome 2 map 6445720...  1721   0.0    2
(AU268867) Dictyostelium discoideum vegetative cDNA clone:VS...  1231   0.0    1
(C94465) Dictyostelium discoideum slug cDNA, clone SSL135.       1164   0.0    1
(AU268866) Dictyostelium discoideum vegetative cDNA clone:VS...  1033   0.0    1
(AU074499) Dictyostelium discoideum slug cDNA, clone SSL135.      222   2e-69  2
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...    36   0.002  16
(AC116982) Dictyostelium discoideum chromosome 2 map 3622643...    40   0.003  12
(AC117176) Dictyostelium discoideum chromosome 2 map 5018074...    40   0.018  11
(AC175887) Strongylocentrotus purpuratus clone R3-15B18, WOR...    42   0.020  9
(AC161570) Mus musculus chromosome 5, clone RP23-36O9, compl...    42   0.029  2
dna update 2009. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116979_95(AC116979|pid:none) Dictyostelium discoideum chromoso...   575   0.0
AM439554_1(AM439554|pid:none) Vitis vinifera contig VV78X007635....    37   0.84
(Q60393) RecName: Full=Botulinum neurotoxin type G;          Sho...    37   1.4
S39791(S39791)neurotoxin - Clostridium botulinum                       37   1.4
CR954253_1830(CR954253|pid:none) Lactobacillus delbrueckii subsp...    35   3.2
FJ446695_1(FJ446695|pid:none) Saccharomyces cerevisiae strain UC...    35   3.2
CP000412_1519(CP000412|pid:none) Lactobacillus delbrueckii subsp...    35   3.2
AM285311_43(AM285311|pid:none) Spiroplasma citri GII3-3X chromos...    35   4.2
(Q5UQL2) RecName: Full=Putative F-box and FNIP repeat-containing...    35   4.2
AP006627_344(AP006627|pid:none) Bacillus clausii KSM-K16 DNA, co...    34   7.1
protein update 2009. 3.24
PSORT 

 psg: 0.56  gvh: 0.60  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: peroxisomal
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar

>> prediction for VSI548 is cyt
5' end seq. ID VSI548F
5' end seq. 
>VSI548F.Seq
GGCAAAAAAACTAATTAGAAGTGAGGATGGTGTAAAATTTTATGATGATGAATTTATTTT
TTACCAAGAAGGTAACATCCCTGTGATTGTAACATTTCCACATAGTGGTAAATTAAAATT
AAGATATTCCAATAAGAAAATCAGGATGTATTGATCCAGATTGGTGGACAGAGGATTTAG
CAATTTGTATGATTCAAGCATTTAATTGTATTTCATGTTGTTGTGGTTGTACTGTAAAAG
ATAAAGATGATAATAAAGCTGAATGTTGTAAACCATCTTCATCATCATCATCGTGTTCTC
CAACAACAACATCTGAAGAATGTTCATGTTGTAATATTAATATAAGTTCAAATAAAGTCG
ATTACAAGTTAGAATGCCCAAGACCATATATTGTATATTCAAAGATTCATAGAGAAATGT
GTGATTTTAATAGAGGTGAATCGAGTGCCTTTGAAGATGAACGTATGAAACCACATTATC
AATTATATCATGATACAATTAAATCATTTATTTCAAAGATTAAATCAGAGA---------
-
Length of 5' end seq. 531
3' end seq. ID VSI548Z
3' end seq. 
>VSI548Z.Seq
----------GTTAGAATGCCCAAGACCATATATTGTATATTCAAAGATTCATAGAGAAA
TGTGTGATTTTAATAGAGGTGAATCGAGTGCCTTTGAAGATGAACGTATGAAACCACATT
ATCAATTATATCATGATACAATTAAATCATTTATTTCAAAGATTAAATCAGAGAATCCTC
AATACTATAATGGTGGTAAAGTTACATTATTGGATATTCACGGTCAATCGGATATACCAT
CCCATATATTACGTGGTACAAAAGATACAAATACAGTTAAACCAATGTTAAAACATTTAG
GTAATGATTCATTCATTGGTGAGAATAGTATTTTTGGGTACCTTTTAAATGTTGGTGGTA
TTGATGTATTCCCATTAAATAATTTACCAAGTGATCAAACTACAGATCCAACAAATCCAA
AATATGTACCACCAAATAAAATACCACATAACAAAAATGAATCTTATAATCCATTAAATC
CATACATTGAACATCCAGAATTTAATGGAGCATTCACAGTTATAAATTATTCAAAAAATT
ATACAATCAATTGTATTCAAGTTGAAATTGGTTATTTATGGAGATCAACAATTGAAAACC
GTATTAAATTTTCACGTACTTTAAGATCTTCAATTTTAAATTTTATTAATAATTATGTTT
AAAATAAAAAATAATAATAATAAAATAAATAAATAAATAAAACAATG
Length of 3' end seq. 697
Connected seq. ID VSI548P
Connected seq. 
>VSI548P.Seq
GGCAAAAAAACTAATTAGAAGTGAGGATGGTGTAAAATTTTATGATGATGAATTTATTTT
TTACCAAGAAGGTAACATCCCTGTGATTGTAACATTTCCACATAGTGGTAAATTAAAATT
AAGATATTCCAATAAGAAAATCAGGATGTATTGATCCAGATTGGTGGACAGAGGATTTAG
CAATTTGTATGATTCAAGCATTTAATTGTATTTCATGTTGTTGTGGTTGTACTGTAAAAG
ATAAAGATGATAATAAAGCTGAATGTTGTAAACCATCTTCATCATCATCATCGTGTTCTC
CAACAACAACATCTGAAGAATGTTCATGTTGTAATATTAATATAAGTTCAAATAAAGTCG
ATTACAAGTTAGAATGCCCAAGACCATATATTGTATATTCAAAGATTCATAGAGAAATGT
GTGATTTTAATAGAGGTGAATCGAGTGCCTTTGAAGATGAACGTATGAAACCACATTATC
AATTATATCATGATACAATTAAATCATTTATTTCAAAGATTAAATCAGAGA---------
-GTTAGAATGCCCAAGACCATATATTGTATATTCAAAGATTCATAGAGAAATGTGTGATT
TTAATAGAGGTGAATCGAGTGCCTTTGAAGATGAACGTATGAAACCACATTATCAATTAT
ATCATGATACAATTAAATCATTTATTTCAAAGATTAAATCAGAGAATCCTCAATACTATA
ATGGTGGTAAAGTTACATTATTGGATATTCACGGTCAATCGGATATACCATCCCATATAT
TACGTGGTACAAAAGATACAAATACAGTTAAACCAATGTTAAAACATTTAGGTAATGATT
CATTCATTGGTGAGAATAGTATTTTTGGGTACCTTTTAAATGTTGGTGGTATTGATGTAT
TCCCATTAAATAATTTACCAAGTGATCAAACTACAGATCCAACAAATCCAAAATATGTAC
CACCAAATAAAATACCACATAACAAAAATGAATCTTATAATCCATTAAATCCATACATTG
AACATCCAGAATTTAATGGAGCATTCACAGTTATAAATTATTCAAAAAATTATACAATCA
ATTGTATTCAAGTTGAAATTGGTTATTTATGGAGATCAACAATTGAAAACCGTATTAAAT
TTTCACGTACTTTAAGATCTTCAATTTTAAATTTTATTAATAATTATGTTTAAAATAAAA
AATAATAATAATAAAATAAATAAATAAATAAAACAATG
Length of connected seq. 1228
Full length Seq ID VSI548E
Full length Seq. 
>VSI548E.Seq
GGCAAAAAAACTAATTAGAAGTGAGGATGGTGTAAAATTTTATGATGATGAATTTATTTT
TTACCAAGAAGGTAACATCCCTGTGATTGTAACATTTCCACATAGTGGTAAATTAAAATT
AAGATATTCCAATAAGAAAATCAGGATGTATTGATCCAGATTGGTGGACAGAGGATTTAG
CAATTTGTATGATTCAAGCATTTAATTGTATTTCATGTTGTTGTGGTTGTACTGTAAAAG
ATAAAGATGATAATAAAGCTGAATGTTGTAAACCATCTTCATCATCATCATCGTGTTCTC
CAACAACAACATCTGAAGAATGTTCATGTTGTAATATTAATATAAGTTCAAATAAAGTCG
ATTACAAGTTAGAATGCCCAAGACCATATATTGTATATTCAAAGATTCATAGAGAAATGT
GTGATTTTAATAGAGGTGAATCGAGTGCCTTTGAAGATGAACGTATGAAACCACATTATC
AATTATATCATGATACAATTAAATCATTTATTTCAAAGATTAAATCAGAGAATCCTCAAT
ACTATAATGGTGGTAAAGTTACATTATTGGATATTCACGGTCAATCGGATATACCATCCC
ATATATTACGTGGTACAAAAGATACAAATACAGTTAAACCAATGTTAAAACATTTAGGTA
ATGATTCATTCATTGGTGAGAATAGTATTTTTGGGTACCTTTTAAATGTTGGTGGTATTG
ATGTATTCCCATTAAATAATTTACCAAGTGATCAAACTACAGATCCAACAAATCCAAAAT
ATGTACCACCAAATAAAATACCACATAACAAAAATGAATCTTATAATCCATTAAATCCAT
ACATTGAACATCCAGAATTTAATGGAGCATTCACAGTTATAAATTATTCAAAAAATTATA
CAATCAATTGTATTCAAGTTGAAATTGGTTATTTATGGAGATCAACAATTGAAAACCGTA
TTAAATTTTCACGTACTTTAAGATCTTCAATTTTAAATTTTATTAATAATTATGTTTAAA
ATAAAAAATAATAATAATAAAATAAATAAATAAATAAAACAATG
Length of full length seq. 1064