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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI530
Library VS
(Link to library)
Clone ID VSI530
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13793-1
Original site URL
Representative seq. ID VSI530P
(Link to Original site)
Representative DNA sequence 
>VSI530 (VSI530Q) /CSM/VS/VSI5-B/VSI530Q.Seq.d/
CATGAACAGTGCTGATAATTCTGGTGCCAAAAATTTATACGTCATTGCCGTCAAAGGTAT
CAAAGGTCGTTTAAATAGATTACCATCCGCAGGTGTTGGTGATATGGTCATGGCTACCGT
CAAAAAGGGTAAACCAGAATTAAGAAAGAAAGTTTGCACTGGTTTAGTTGTCAGACAAAG
AAAACACTGGAAGAGAAAGGATGGTGTTTACATCTATTTCGAAGATAACGCTGGTGTTAT
GTGTAATCCAAAAGGTGAAGTCAAAGGTAACATTCTCGGACCAGTCGCCAAGGAATGCTC
CGATTTATGGCCAAAGGTTGCCACCAATGCCGGTACCATTGTTTAAATAAATACACATAA
AGTTAAAACAATATAAAAAAAAAAXXXXXXXXXXCATGAACAGTGCTGATAATTCTGGTG
CCAAAAATTTATACGTCATTGCCGTCAAAGGTATCAAAGGTCGTTTAAATAGATTACCAT
CCGCAGGTGTTGGTGATATGGTCATGGCTACCGTCAAAAAGGGTAAACCAGAATTAAGAA
AGAAAGTTTGCACTGGTTTAGTTGTCAGACAAAGAAAACACTGGAAGAGAAAGGATGGTG
TTTACATCTATTTCGAAGATAACGCTGGTGTTATGTGTAATCCAAAAGGTGAAGTCAAAG
GTAACATTCTCGGACCAGTCGCCAAGGAATGCTCCGATTTATGGCCAAAGGTTGCCACCA
ATGCCGGTACCATTGTTTAAATAAATACACATAAAGTTAAAACAATA
sequence update 2001. 3.22
Translated Amino Acid sequence 
*tvliilvpkiytslpskvskvv*idyhpqvlviwswlpskrvnqn*erkfalv*lsdke
ntgrermvftsiskitlvlcviqkvkskvtfsdqsprnapiygqrlppmpvplfk*IHIK
LKQYKKK---

---MNSADNSGAKNLYVIAVKGIKGRLNRLPSAGVGDMVMATVKKGKPELRKKVCTGLVV
RQRKHWKRKDGVYIYFEDNAGVMCNPKGEVKGNILGPVAKECSDLWPKVATNAGTIV*in
thkvkti
Translated Amino Acid sequence (All Frames) 
Frame A:
heqc**fwcqkfirhcrqryqrsfk*itirrcw*yghgyrqkg*trikkeslhwfscqtk
ktleekgwclhlfrr*rwcyv*skr*sqr*hsrtsrqgmlrfmakgchqcryhclnkyt*
s*nnikkk---

---heqc**fwcqkfirhcrqryqrsfk*itirrcw*yghgyrqkg*trikkeslhwfsc
qtkktleekgwclhlfrr*rwcyv*skr*sqr*hsrtsrqgmlrfmakgchqcryhclnk
yt*s*nn

Frame B:
mnsadnsgaknlyviavkgikgrlnrlpsagvgdmvmatvkkgkpelrkkvctglvvrqr
khwkrkdgvyiyfednagvmcnpkgevkgnilgpvakecsdlwpkvatnagtiv*inthk
vkti*kk---

---MNSADNSGAKNLYVIAVKGIKGRLNRLPSAGVGDMVMATVKKGKPELRKKVCTGLVV
RQRKHWKRKDGVYIYFEDNAGVMCNPKGEVKGNILGPVAKECSDLWPKVATNAGTIV*in
thkvkti

Frame C:
*tvliilvpkiytslpskvskvv*idyhpqvlviwswlpskrvnqn*erkfalv*lsdke
ntgrermvftsiskitlvlcviqkvkskvtfsdqsprnapiygqrlppmpvplfk*IHIK
LKQYKKK---

---*tvliilvpkiytslpskvskvv*idyhpqvlviwswlpskrvnqn*erkfalv*ls
dkentgrermvftsiskitlvlcviqkvkskvtfsdqsprnapiygqrlppmpvplfk*i
hiklkq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK191 (VSK191Q) /CSM/VS/VSK1-D/VSK191Q.Seq.d/                        741   0.0
VSJ725 (VSJ725Q) /CSM/VS/VSJ7-B/VSJ725Q.Seq.d/                        741   0.0
VSJ286 (VSJ286Q) /CSM/VS/VSJ2-D/VSJ286Q.Seq.d/                        741   0.0
VSJ211 (VSJ211Q) /CSM/VS/VSJ2-A/VSJ211Q.Seq.d/                        741   0.0
VSJ195 (VSJ195Q) /CSM/VS/VSJ1-D/VSJ195Q.Seq.d/                        741   0.0
VSJ186 (VSJ186Q) /CSM/VS/VSJ1-D/VSJ186Q.Seq.d/                        741   0.0
VSI870 (VSI870Q) /CSM/VS/VSI8-C/VSI870Q.Seq.d/                        741   0.0
VSI530 (VSI530Q) /CSM/VS/VSI5-B/VSI530Q.Seq.d/                        741   0.0
VSI206 (VSI206Q) /CSM/VS/VSI2-A/VSI206Q.Seq.d/                        741   0.0
VSI106 (VSI106Q) /CSM/VS/VSI1-A/VSI106Q.Seq.d/                        741   0.0
own update 2001.11.13
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU270782) Dictyostelium discoideum vegetative cDNA clone:VS...   741   0.0    1
(AU270371) Dictyostelium discoideum vegetative cDNA clone:VS...   741   0.0    1
(AU269700) Dictyostelium discoideum vegetative cDNA clone:VS...   741   0.0    1
(AU269576) Dictyostelium discoideum vegetative cDNA clone:VS...   741   0.0    1
(AU269558) Dictyostelium discoideum vegetative cDNA clone:VS...   741   0.0    1
(AU269540) Dictyostelium discoideum vegetative cDNA clone:VS...   741   0.0    1
(AU269371) Dictyostelium discoideum vegetative cDNA clone:VS...   741   0.0    1
(AU268836) Dictyostelium discoideum vegetative cDNA clone:VS...   741   0.0    1
(AU268316) Dictyostelium discoideum vegetative cDNA clone:VS...   741   0.0    1
(AU268155) Dictyostelium discoideum vegetative cDNA clone:VS...   741   0.0    1
dna update 2009. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR382130_420(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   183   6e-45
(Q6PC14) RecName: Full=60S ribosomal protein L23;  &AY684289_1(A...   182   7e-45
AK012694_1(AK012694|pid:none) Mus musculus 10, 11 days embryo wh...   182   1e-44
BC102556_1(BC102556|pid:none) Bos taurus ribosomal protein L23, ...   181   2e-44
BT058333_1(BT058333|pid:none) Salmo salar clone Contig3997 60S r...   181   2e-44
EU042114_1(EU042114|pid:none) Epinephelus coioides ribosomal pro...   181   2e-44
BC003518_1(BC003518|pid:none) Homo sapiens, Similar to ribosomal...   181   2e-44
AC022457_14(AC022457|pid:none) Oryza sativa chromosome 10 BAC OS...   180   4e-44
BT036711_1(BT036711|pid:none) Zea mays full-length cDNA clone ZM...   180   4e-44
BC073541_1(BC073541|pid:none) Xenopus laevis MGC82808 protein, m...   180   5e-44
protein update 2009. 3.24
PSORT 

 psg: 0.63  gvh: 0.32  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.07
 nuc: 0.29  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VSI530 is nuc
5' end seq. ID VSI530F
5' end seq. 
>VSI530F.Seq
CATGAACAGTGCTGATAATTCTGGTGCCAAAAATTTATACGTCATTGCCGTCAAAGGTAT
CAAAGGTCGTTTAAATAGATTACCATCCGCAGGTGTTGGTGATATGGTCATGGCTACCGT
CAAAAAGGGTAAACCAGAATTAAGAAAGAAAGTTTGCACTGGTTTAGTTGTCAGACAAAG
AAAACACTGGAAGAGAAAGGATGGTGTTTACATCTATTTCGAAGATAACGCTGGTGTTAT
GTGTAATCCAAAAGGTGAAGTCAAAGGTAACATTCTCGGACCAGTCGCCAAGGAATGCTC
CGATTTATGGCCAAAGGTTGCCACCAATGCCGGTACCATTGTTTAAATAAATACACATAA
AGTTAAAACAATATAAAAAAAAAA----------
Length of 5' end seq. 384
3' end seq. ID VSI530Z
3' end seq. 
>VSI530Z.Seq
----------CATGAACAGTGCTGATAATTCTGGTGCCAAAAATTTATACGTCATTGCCG
TCAAAGGTATCAAAGGTCGTTTAAATAGATTACCATCCGCAGGTGTTGGTGATATGGTCA
TGGCTACCGTCAAAAAGGGTAAACCAGAATTAAGAAAGAAAGTTTGCACTGGTTTAGTTG
TCAGACAAAGAAAACACTGGAAGAGAAAGGATGGTGTTTACATCTATTTCGAAGATAACG
CTGGTGTTATGTGTAATCCAAAAGGTGAAGTCAAAGGTAACATTCTCGGACCAGTCGCCA
AGGAATGCTCCGATTTATGGCCAAAGGTTGCCACCAATGCCGGTACCATTGTTTAAATAA
ATACACATAAAGTTAAAACAATA
Length of 3' end seq. 373
Connected seq. ID VSI530P
Connected seq. 
>VSI530P.Seq
CATGAACAGTGCTGATAATTCTGGTGCCAAAAATTTATACGTCATTGCCGTCAAAGGTAT
CAAAGGTCGTTTAAATAGATTACCATCCGCAGGTGTTGGTGATATGGTCATGGCTACCGT
CAAAAAGGGTAAACCAGAATTAAGAAAGAAAGTTTGCACTGGTTTAGTTGTCAGACAAAG
AAAACACTGGAAGAGAAAGGATGGTGTTTACATCTATTTCGAAGATAACGCTGGTGTTAT
GTGTAATCCAAAAGGTGAAGTCAAAGGTAACATTCTCGGACCAGTCGCCAAGGAATGCTC
CGATTTATGGCCAAAGGTTGCCACCAATGCCGGTACCATTGTTTAAATAAATACACATAA
AGTTAAAACAATATAAAAAAAAAA----------CATGAACAGTGCTGATAATTCTGGTG
CCAAAAATTTATACGTCATTGCCGTCAAAGGTATCAAAGGTCGTTTAAATAGATTACCAT
CCGCAGGTGTTGGTGATATGGTCATGGCTACCGTCAAAAAGGGTAAACCAGAATTAAGAA
AGAAAGTTTGCACTGGTTTAGTTGTCAGACAAAGAAAACACTGGAAGAGAAAGGATGGTG
TTTACATCTATTTCGAAGATAACGCTGGTGTTATGTGTAATCCAAAAGGTGAAGTCAAAG
GTAACATTCTCGGACCAGTCGCCAAGGAATGCTCCGATTTATGGCCAAAGGTTGCCACCA
ATGCCGGTACCATTGTTTAAATAAATACACATAAAGTTAAAACAATA
Length of connected seq. 757
Full length Seq ID -
Full length Seq. -
Length of full length seq. -