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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI464
Library VS
(Link to library)
Clone ID VSI464
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10256-1
Original site URL
Representative seq. ID VSI464F
(Link to Original site)
Representative DNA sequence 
>VSI464 (VSI464Q) /CSM/VS/VSI4-C/VSI464Q.Seq.d/
AAGCTTTTGAACTCTTTGATAAAGATAGAACTGGTTTCATTAAAAAGGATGCCTTAAAAA
CCACCTGTAAACAATTTGGTGTTTTTGTTATGGAAGATCAATTAGATGCCATGTTTGCTG
AAGCTGATACCACCAAATCTGGTGCTATTGGTTTCCCAGAATTTATGTCAATGATGTCCC
GTCGTATGAAACAAACTTCAAATGAACAAATTTTAATGAACGCTTTTAAAACTTTTGATC
CAGAAGGTAATGGTTACATCTTAACAAAAGATTTATCTAAAGCCTTAACAACTTTGGGTG
ATAAATTAACTGAAGCAGAGTTACAAGAATTGTTATCAATTTCAGAAAACGAACAAAAGC
AAGTTAAATATGACCTTTTCGTTAATACTCTCTTCAGTAAAAAATAAAGTGATTATTAAA
TAGTGGAAAAAAAAAAXXXXXXXXXX
sequence update 2001. 3.22
Translated Amino Acid sequence 
AFELFDKDRTGFIKKDALKTTCKQFGVFVMEDQLDAMFAEADTTKSGAIGFPEFMSMMSR
RMKQTSNEQILMNAFKTFDPEGNGYILTKDLSKALTTLGDKLTEAELQELLSISENEQKQ
VKYDLFVNTLFSKK*sdy*ivekk---
Translated Amino Acid sequence (All Frames) 
Frame A:
kllnslikielvslkrmp*kppvnnlvfllwkin*mpcllklippnlvllvsqnlcq*cp
vv*nklqmnkf**tllklliqkvmvts*qkiylkp*qlwvin*lkqsykncyqfqktnks
klnmtfslilssvknkviik*wkkk---


Frame B:
sf*tl**r*nwfh*kgclknhl*tiwcfcygrsirchvc*s*yhqiwcywfpriyvndvp
syetnfk*tnfnerf*nf*srr*wlhlnkrfi*slnnfg**in*srvtrivinfrkrtka
s*i*pfr*yslq*kik*llnsgkk---


Frame C:
AFELFDKDRTGFIKKDALKTTCKQFGVFVMEDQLDAMFAEADTTKSGAIGFPEFMSMMSR
RMKQTSNEQILMNAFKTFDPEGNGYILTKDLSKALTTLGDKLTEAELQELLSISENEQKQ
VKYDLFVNTLFSKK*sdy*ivekk---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI464 (VSI464Q) /CSM/VS/VSI4-C/VSI464Q.Seq.d/                        844   0.0
VSI369 (VSI369Q) /CSM/VS/VSI3-C/VSI369Q.Seq.d/                        844   0.0
SSJ450 (SSJ450Q) /CSM/SS/SSJ4-C/SSJ450Q.Seq.d/                        844   0.0
SSI238 (SSI238Q) /CSM/SS/SSI2-B/SSI238Q.Seq.d/                        844   0.0
SSG876 (SSG876Q) /CSM/SS/SSG8-D/SSG876Q.Seq.d/                        844   0.0
SSA512 (SSA512Q) /CSM/SS/SSA5-A/SSA512Q.Seq.d/                        844   0.0
SSC193 (SSC193Q) /CSM/SS/SSC1-D/SSC193Q.Seq.d/                        837   0.0
SSK379 (SSK379Q) /CSM/SS/SSK3-D/SSK379Q.Seq.d/                        827   0.0
SSB548 (SSB548Q) /CSM/SS/SSB5-B/SSB548Q.Seq.d/                        821   0.0
SSD351 (SSD351Q) /CSM/SS/SSD3-C/SSD351Q.Seq.d/                        787   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(M25251) D.discoideum regulatory myosin light chain (rmlc) g...   844   0.0    1
(AU268735) Dictyostelium discoideum vegetative cDNA clone:VS...   844   0.0    1
(AU268589) Dictyostelium discoideum vegetative cDNA clone:VS...   844   0.0    1
(C90197) Dictyostelium discoideum slug cDNA, clone SSI238.        844   0.0    1
(C90001) Dictyostelium discoideum slug cDNA, clone SSG876.        844   0.0    1
(C89656) Dictyostelium discoideum slug cDNA, clone SSA512.        844   0.0    1
(C91516) Dictyostelium discoideum slug cDNA, clone SSK379.        827   0.0    1
(AU037162) Dictyostelium discoideum slug cDNA, clone SSB548.      821   0.0    1
(AC116979) Dictyostelium discoideum chromosome 2 map 6445720...   460   0.0    2
(C92290) Dictyostelium discoideum slug cDNA, clone SSD351.        787   0.0    1
dna update 2009. 7.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P13833) RecName: Full=Myosin regulatory light chain; AltName: F...   265   5e-70
AC116979_41(AC116979|pid:none) Dictyostelium discoideum chromoso...   261   7e-69
AB076705_1(AB076705|pid:none) Physarum polycephalum RMLC mRNA fo...   152   3e-36
(O97341) RecName: Full=Calmodulin;          Short=CaM;  &Y18166_...   106   2e-22
(P27165) RecName: Full=Calmodulin;          Short=CaM;  &(Q71UH5...   105   6e-22
(Q9D6P8) RecName: Full=Calmodulin-like protein 3;  &AK010118_1(A...   105   6e-22
CR940348_242(CR940348|pid:none) Theileria annulata strain Ankara...   104   8e-22
AB222133_1(AB222133|pid:none) Pan troglodytes verus calm1 mRNA f...   104   1e-21
MCSFCU(JK0011)calmodulin - sea cucumber (Stichopus japonicus)         104   1e-21
AY893101_1(AY893101|pid:none) Synthetic construct Homo sapiens c...   104   1e-21
protein update 2009. 3.24
PSORT 

 psg: 0.75  gvh: 0.40  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for VSI464 is cyt
5' end seq. ID VSI464F
5' end seq. 
>VSI464F.Seq
AAGCTTTTGAACTCTTTGATAAAGATAGAACTGGTTTCATTAAAAAGGATGCCTTAAAAA
CCACCTGTAAACAATTTGGTGTTTTTGTTATGGAAGATCAATTAGATGCCATGTTTGCTG
AAGCTGATACCACCAAATCTGGTGCTATTGGTTTCCCAGAATTTATGTCAATGATGTCCC
GTCGTATGAAACAAACTTCAAATGAACAAATTTTAATGAACGCTTTTAAAACTTTTGATC
CAGAAGGTAATGGTTACATCTTAACAAAAGATTTATCTAAAGCCTTAACAACTTTGGGTG
ATAAATTAACTGAAGCAGAGTTACAAGAATTGTTATCAATTTCAGAAAACGAACAAAAGC
AAGTTAAATATGACCTTTTCGTTAATACTCTCTTCAGTAAAAAATAAAGTGATTATTAAA
TAGTGGAAAAAAAAAA----------
Length of 5' end seq. 436
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -