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Dicty_cDB
Contig List | Clone List | cDNA Library List
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Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI457
Library VS
(Link to library)
Clone ID VSI457
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U16136-1
Original site URL
Representative seq. ID VSI457P
(Link to Original site)
Representative DNA sequence 
>VSI457 (VSI457Q) /CSM/VS/VSI4-C/VSI457Q.Seq.d/
AATAAATACTCTATAATATATTATATATTAGTTAATAATAATAATAATAATAATAATAAT
AATAATAGTAATAGTAAATATATAAACAAAATACAAATAAAAATATAAAAAATGAATAAT
TTACGTGTTAAAGATGATTTCGATTATGAAGATATTATGGATGAAGAGTCCTTAATGGAT
AATATGAATGAACATGGTAAAACTTTAGTTCACACTGAATTCTTTAATAGTTTTGAAGAT
GATTTTGAAGATGAATTTGAAGAAGATAGTTTAAAATTCAACGTTAAAAATGATAACAAT
AATAACAACAACTTCAAAAAACCAGAATCAAAATAAACAACACCAGACAAAAGTNAAAAA
AAAAXXXXXXXXXXGAGATAATCAAATAAATACTCTATAATATATTATATATTAGTTAAT
AATAATAATAATAATAATAATAATAATAATAGTAATAGTAAATATATAAACAAAATACAA
ATAAAAATATAAAAAATGAATAATTTACGTGTTAAAGATGATTTCGATTATGAAGATATT
ATGGATGAAGAGTCCTTAATGGATAATATGAATGAACATGGTAAAACTTTAGTTCACACT
GAATTCTTTAATAGTTTTGAAGATGATTTTGAAGATGAATTTGAAGAAGATAGTTTAAAA
TTCAACGNNAAAAATG
sequence update 2001. 3.22
Translated Amino Acid sequence 
intl*yiiy*liiiiiiiiiiivivni*tkyk*kykk*iiyvlkmisimkilwmksp*wi
i*mnmvkl*ftlnslivlkmilkmnlkkiv*NSTLKMITIITTTSKNQNQNKQHQTKVKK
K---

---DNQINTL*yiiy*liiiiiiiiiiivivni*tkyk*kykk*iiyvlkmisimkilwm
ksp*wii*mnmvkl*ftlnslivlkmilkmnlkkiv*nstxkm
Translated Amino Acid sequence (All Frames) 
Frame A:
nkysiiyyilvnnnnnnnnnnnsnskyinkiqiki*kmnnlrvkddfdyedimdeeslmd
nmnehgktlvhteffnsfeddfedefeedslkfnvkndnnnnnnfkkpesk*ttpdksxk
k---

---eiik*ilynilyis***************iykqntnknikne*ftc*r*frl*ryyg
*rvlng*ye*tw*nfssh*il**f*r*f*r*i*rr*fkiqrxk

Frame B:
intl*yiiy*liiiiiiiiiiivivni*tkyk*kykk*iiyvlkmisimkilwmksp*wi
i*mnmvkl*ftlnslivlkmilkmnlkkiv*NSTLKMITIITTTSKNQNQNKQHQTKVKK
K---

---r*snkysiiyyilvnnnnnnnnnnnsnskyinkiqiki*kmnnlrvkddfdyedimd
eeslmdnmnehgktlvhteffnsfeddfedefeedslkfnxkn

Frame C:
*ilynilyis***************iykqntnknikne*ftc*r*frl*ryyg*rvlng*
ye*tw*nfssh*il**f*r*f*r*i*rr*fkiqr*k**q**qqlqktrikinntrqkxkk
---

---DNQINTL*yiiy*liiiiiiiiiiivivni*tkyk*kykk*iiyvlkmisimkilwm
ksp*wii*mnmvkl*ftlnslivlkmilkmnlkkiv*nstxkm
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI457 (VSI457Q) /CSM/VS/VSI4-C/VSI457Q.Seq.d/                        464   e-130
AFL553 (AFL553Q) /CSM/AF/AFL5-C/AFL553Q.Seq.d/                        460   e-128
SSJ437 (SSJ437Q) /CSM/SS/SSJ4-B/SSJ437Q.Seq.d/                        448   e-125
SSH190 (SSH190Q) /CSM/SS/SSH1-D/SSH190Q.Seq.d/                        387   e-106
FC-BP22 (FC-BP22Q) /CSM/FC/FC-BP/FC-BP22Q.Seq.d/                      230   2e-59
VHN756 (VHN756Q) /CSM/VH/VHN7-C/VHN756Q.Seq.d/                         40   0.043
VFN225 (VFN225Q) /CSM/VF/VFN2-B/VFN225Q.Seq.d/                         40   0.043
VFK542 (VFK542Q) /CSM/VF/VFK5-B/VFK542Q.Seq.d/                         40   0.043
VFI527 (VFI527Q) /CSM/VF/VFI5-B/VFI527Q.Seq.d/                         40   0.043
VFB230 (VFB230Q) /CSM/VF/VFB2-B/VFB230Q.Seq.d/                         40   0.043
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268725) Dictyostelium discoideum vegetative cDNA clone:VS...   329   8e-86  1
(AU268724) Dictyostelium discoideum vegetative cDNA clone:VS...   329   8e-86  1
(BJ346654) Dictyostelium discoideum cDNA clone:dda24i13, 3' ...   329   8e-86  1
(AU073434) Dictyostelium discoideum slug cDNA, clone SSH190.      220   5e-53  1
(AU074066) Dictyostelium discoideum slug cDNA, clone SSJ437.      218   2e-52  1
(AU284919) Dictyostelium discoideum gamete cDNA clone:FC-BP2...   216   9e-52  1
(FN366098) Schistosoma mansoni genome sequence supercontig S...    40   1e-05  4
(FN366038) Schistosoma mansoni genome sequence supercontig S...    40   1e-05  4
(FN365938) Schistosoma mansoni genome sequence supercontig S...    40   2e-05  4
(FN363910) Schistosoma mansoni genome sequence supercontig S...    40   4e-05  4
dna update 2009. 7.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC146564_26(AC146564|pid:none) Medicago truncatula clone mth2-67...    33   5.6
AC137510_17(AC137510|pid:none) Medicago truncatula clone mth2-8m...    33   5.6
protein update 2009. 3.24
PSORT 

 psg: 0.94  gvh: 0.36  alm: 0.64  top: 0.77  tms: 0.00  mit: 0.41  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VSI457 is mit
5' end seq. ID VSI457F
5' end seq. 
>VSI457F.Seq
AATAAATACTCTATAATATATTATATATTAGTTAATAATAATAATAATAATAATAATAAT
AATAATAGTAATAGTAAATATATAAACAAAATACAAATAAAAATATAAAAAATGAATAAT
TTACGTGTTAAAGATGATTTCGATTATGAAGATATTATGGATGAAGAGTCCTTAATGGAT
AATATGAATGAACATGGTAAAACTTTAGTTCACACTGAATTCTTTAATAGTTTTGAAGAT
GATTTTGAAGATGAATTTGAAGAAGATAGTTTAAAATTCAACGTTAAAAATGATAACAAT
AATAACAACAACTTCAAAAAACCAGAATCAAAATAAACAACACCAGACAAAAGTNAAAAA
AAAA----------
Length of 5' end seq. 364
3' end seq. ID VSI457Z
3' end seq. 
>VSI457Z.Seq
----------GAGATAATCAAATAAATACTCTATAATATATTATATATTAGTTAATAATA
ATAATAATAATAATAATAATAATAATAGTAATAGTAAATATATAAACAAAATACAAATAA
AAATATAAAAAATGAATAATTTACGTGTTAAAGATGATTTCGATTATGAAGATATTATGG
ATGAAGAGTCCTTAATGGATAATATGAATGAACATGGTAAAACTTTAGTTCACACTGAAT
TCTTTAATAGTTTTGAAGATGATTTTGAAGATGAATTTGAAGAAGATAGTTTAAAATTCA
ACGNNAAAAATG
Length of 3' end seq. 302
Connected seq. ID VSI457P
Connected seq. 
>VSI457P.Seq
AATAAATACTCTATAATATATTATATATTAGTTAATAATAATAATAATAATAATAATAAT
AATAATAGTAATAGTAAATATATAAACAAAATACAAATAAAAATATAAAAAATGAATAAT
TTACGTGTTAAAGATGATTTCGATTATGAAGATATTATGGATGAAGAGTCCTTAATGGAT
AATATGAATGAACATGGTAAAACTTTAGTTCACACTGAATTCTTTAATAGTTTTGAAGAT
GATTTTGAAGATGAATTTGAAGAAGATAGTTTAAAATTCAACGTTAAAAATGATAACAAT
AATAACAACAACTTCAAAAAACCAGAATCAAAATAAACAACACCAGACAAAAGTNAAAAA
AAAA----------GAGATAATCAAATAAATACTCTATAATATATTATATATTAGTTAAT
AATAATAATAATAATAATAATAATAATAATAGTAATAGTAAATATATAAACAAAATACAA
ATAAAAATATAAAAAATGAATAATTTACGTGTTAAAGATGATTTCGATTATGAAGATATT
ATGGATGAAGAGTCCTTAATGGATAATATGAATGAACATGGTAAAACTTTAGTTCACACT
GAATTCTTTAATAGTTTTGAAGATGATTTTGAAGATGAATTTGAAGAAGATAGTTTAAAA
TTCAACGNNAAAAATG
Length of connected seq. 666
Full length Seq ID -
Full length Seq. -
Length of full length seq. -