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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI449
Library VS
(Link to library)
Clone ID VSI449
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U06683-1
Original site URL
Representative seq. ID VSI449P
(Link to Original site)
Representative DNA sequence 
>VSI449 (VSI449Q) /CSM/VS/VSI4-C/VSI449Q.Seq.d/
CAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGTTATCCAATTTTTTCAGATTTTT
GTACATGTATTTTTGATAAACCATTAGAATTATGTGAACCATACAGAAAAGATTATTTAG
AATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCATCAGAAAATTACCATAAAGGTG
GTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTAAAAGAGCTCAAGAATATGATCCAA
AAAAAGGAAAATCTGAATAAATTCAATTTTTTTTTTAATAGTAATAAATAATAATAATTA
ATAACTAATAATAATAATAATAATAATATAATTTAGTGATAATACGATAAAACACAAACA
ACAAAAAAAAAAXXXXXXXXXXCAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGT
NATCCAATTTTTTCAGATTTTTGTACATGTATTTTTGATAAACCATTAGAATTATGTGAA
CCATACAGAAAAGATTATTTAGAATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCA
TCAGAAAATTACCATAAAGGTGGTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTAAA
AGAGCTCAAGAATATGATCCAAAAAAAG
sequence update 2001. 3.22
Translated Amino Acid sequence 
kwywclgwiwymlsnffrflymyf**tirim*tiqkrlfrmsp*c*ri*esiirklp*rw
*gkn*qs*rsn*kssri*skkrki*insifflivinnnn**liiiiiii*FSDNTIKHKQ
QKK---

---SGIGAWGGSGTCXPIFSDFCTCIFDKPLELCEPYRKDYLECLHNVKEYKSQSSENYH
KGGEERINKVREAIKRAQEYDPKK
Translated Amino Acid sequence (All Frames) 
Frame A:
qvvlvlgvdlvhviqffqifvhvflinh*nyvnhtekii*nvsimlknirvnhqkitikv
vrkeltklekqlkelknmiqkkenlnkfnfffnsnk***litnnnnnnnii***ydktqt
tkkk---

---qvvlvlgvdlvhviqffqifvhvflinh*nyvnhtekii*nvsimlknirvnhqkit
ikvvrkeltklekqlkelknmiqkk

Frame B:
kwywclgwiwymlsnffrflymyf**tirim*tiqkrlfrmsp*c*ri*esiirklp*rw
*gkn*qs*rsn*kssri*skkrki*insifflivinnnn**liiiiiii*FSDNTIKHKQ
QKK---

---kwywclgwiwymxsnffrflymyf**tirim*tiqkrlfrmsp*c*ri*esiirklp
*rw*gkn*qs*rsn*kssri*skk

Frame C:
sgigawggsgtcypifsdfctcifdkplelcepyrkdyleclhnvkeyksqssenyhkgg
eerinkvreaikraqeydpkkgkse*iqfff****iiiinn*******ynlviir*ntnn
kkk---

---SGIGAWGGSGTCXPIFSDFCTCIFDKPLELCEPYRKDYLECLHNVKEYKSQSSENYH
KGGEERINKVREAIKRAQEYDPKK
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI449 (VSI449Q) /CSM/VS/VSI4-C/VSI449Q.Seq.d/                        472   e-132
VSF189 (VSF189Q) /CSM/VS/VSF1-D/VSF189Q.Seq.d/                        472   e-132
VSG455 (VSG455Q) /CSM/VS/VSG4-C/VSG455Q.Seq.d/                        234   1e-60
VHQ456 (VHQ456Q) /CSM/VH/VHQ4-C/VHQ456Q.Seq.d/                         34   2.4
VHH662 (VHH662Q) /CSM/VH/VHH6-C/VHH662Q.Seq.d/                         34   2.4
VHG211 (VHG211Q) /CSM/VH/VHG2-A/VHG211Q.Seq.d/                         34   2.4
VHC465 (VHC465Q) /CSM/VH/VHC4-C/VHC465Q.Seq.d/                         34   2.4
VHB296 (VHB296Q) /CSM/VH/VHB2-D/VHB296Q.Seq.d/                         34   2.4
VFK306 (VFK306Q) /CSM/VF/VFK3-A/VFK306Q.Seq.d/                         34   2.4
VFH740 (VFH740Q) /CSM/VF/VFH7-B/VFH740Q.Seq.d/                         34   2.4
own update 2004. 8. 6
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268713) Dictyostelium discoideum vegetative cDNA clone:VS...   505   e-149  2
(AU264843) Dictyostelium discoideum vegetative cDNA clone:VS...   505   e-149  2
(AU268714) Dictyostelium discoideum vegetative cDNA clone:VS...   482   e-132  1
(AU264844) Dictyostelium discoideum vegetative cDNA clone:VS...   438   e-118  1
(AU266507) Dictyostelium discoideum vegetative cDNA clone:VS...   234   2e-58  2
(EH271153) Gp_mxAA_51H03_M13R mxA Gammarus pulex cDNA clone ...    38   0.017  2
(EH272166) Gp_mxAA_55E02_M13R mxA Gammarus pulex cDNA clone ...    38   0.18   2
(AL671910) Mouse DNA sequence from clone RP23-245M12 on chro...    46   0.37   3
(AC179586) Strongylocentrotus purpuratus clone R3-1029K18, W...    38   0.63   4
(AF528383) Hypochaeris sessiliflora isolate 1 chloroplast tR...    38   0.93   2
dna update 2009. 7.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF084615_1(EF084615|pid:none) Picea sitchensis clone WS02714_K03...    50   5e-05
AK121358_1(AK121358|pid:none) Oryza sativa Japonica Group cDNA c...    49   1e-04
EF144605_1(EF144605|pid:none) Populus trichocarpa clone WS01111_...    47   4e-04
AC005309_6(AC005309|pid:none) Arabidopsis thaliana chromosome 2 ...    46   7e-04
AY088460_1(AY088460|pid:none) Arabidopsis thaliana clone 6853 mR...    46   7e-04
CR954212_50(CR954212|pid:none) Ostreococcus tauri strain OTTH059...    44   0.003
AL353822_11(AL353822|pid:none) Neurospora crassa DNA linkage gro...    39   0.11
CR382132_764(CR382132|pid:none) Yarrowia lipolytica strain CLIB1...    37   0.57
DQ091254_1(DQ091254|pid:none) Fenneropenaeus chinensis thrombosp...    35   1.7
FM992690_314(FM992690|pid:none) Candida dubliniensis CD36 chromo...    35   2.2
protein update 2009. 3.24
PSORT 

 psg: 0.55  gvh: 0.49  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VSI449 is cyt
5' end seq. ID VSI449F
5' end seq. 
>VSI449F.Seq
CAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGTTATCCAATTTTTTCAGATTTTT
GTACATGTATTTTTGATAAACCATTAGAATTATGTGAACCATACAGAAAAGATTATTTAG
AATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCATCAGAAAATTACCATAAAGGTG
GTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTAAAAGAGCTCAAGAATATGATCCAA
AAAAAGGAAAATCTGAATAAATTCAATTTTTTTTTTAATAGTAATAAATAATAATAATTA
ATAACTAATAATAATAATAATAATAATATAATTTAGTGATAATACGATAAAACACAAACA
ACAAAAAAAAAA----------
Length of 5' end seq. 372
3' end seq. ID VSI449Z
3' end seq. 
>VSI449Z.Seq
----------CAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGTNATCCAATTTTT
TCAGATTTTTGTACATGTATTTTTGATAAACCATTAGAATTATGTGAACCATACAGAAAA
GATTATTTAGAATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCATCAGAAAATTAC
CATAAAGGTGGTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTAAAAGAGCTCAAGAA
TATGATCCAAAAAAAG
Length of 3' end seq. 246
Connected seq. ID VSI449P
Connected seq. 
>VSI449P.Seq
CAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGTTATCCAATTTTTTCAGATTTTT
GTACATGTATTTTTGATAAACCATTAGAATTATGTGAACCATACAGAAAAGATTATTTAG
AATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCATCAGAAAATTACCATAAAGGTG
GTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTAAAAGAGCTCAAGAATATGATCCAA
AAAAAGGAAAATCTGAATAAATTCAATTTTTTTTTTAATAGTAATAAATAATAATAATTA
ATAACTAATAATAATAATAATAATAATATAATTTAGTGATAATACGATAAAACACAAACA
ACAAAAAAAAAA----------CAAGTGGTATTGGTGCTTGGGGTGGATCTGGTACATGT
NATCCAATTTTTTCAGATTTTTGTACATGTATTTTTGATAAACCATTAGAATTATGTGAA
CCATACAGAAAAGATTATTTAGAATGTCTCCATAATGTTAAAGAATATAAGAGTCAATCA
TCAGAAAATTACCATAAAGGTGGTGAGGAAAGAATTAACAAAGTTAGAGAAGCAATTAAA
AGAGCTCAAGAATATGATCCAAAAAAAG
Length of connected seq. 618
Full length Seq ID -
Full length Seq. -
Length of full length seq. -