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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI333
Library VS
(Link to library)
Clone ID VSI333
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15331-1
Original site URL
Representative seq. ID VSI333P
(Link to Original site)
Representative DNA sequence 
>VSI333 (VSI333Q) /CSM/VS/VSI3-B/VSI333Q.Seq.d/
GTAATATTTCACACAATATATAAAATGTCATCTTATTTATTCACTAGTGAATCCGTCACC
GAAGGTCATCCAGATAAAATCTGTGATCAAGTATCAGATGCTGTTCTCGATGCTTGTTTA
GCTCAAGATCCATTATCAAAAGTTGCTTGTGAAACTGCAACCAAAACTGGTATGGTTATG
ATTTTAGGTGAAATTACCACCAAAGCCAACGTTGACTATCAAAAAGTTGTTCGTGAAGCT
GTTAAAAAGATTGGTTATGATGACTCATCAAAAGGTTTTGATTACAAAACTTGCAATGTT
TTAGTTGCAATTGAACAACAATCACCAGATATTGCTCAAGGTGTCCACATTGGTAAAGTT
AACCCAGAAGATATTGGTGCTGGTGATCAAGGTCACATGTTTGGTTATGCCACCAACGAA
ACCCCAACTTTAATGCCACTTACTCATTTCCTCGCCTCTGAATTAGTCAACAAATTAACT
GAATTACGTCACAATGGTACCTTAGCCTGGGCTCGTCCAGATGCCAAGACTCAAGTCACT
GTTGAATACAAAAAAGAAGGTCACAAAATCACTCCAGTCAGAGTACXXXXXXXXXXTACC
ATTTGAATCCATCAGGTCGTTTCGTCATTGGTGGCCCAATGGGTGATTNTGGTTTAACCG
GTNGTAANATCATCATTGATTCATATGGNGGTTGGGGTGCNCNCGGTGGTGGTGCTTTNT
CTGGTAAANANGCCACTAAANTCGATCGTTNTGGNGCTTACGCTGCTAAATGGATCGCTA
AATCTTTAGTTGCCNCTGGTCTTGCCGATCGTTGTTTAGTTCAAGTTTCANACTCTATTG
GNGNTGCCAANCCACTCAGTGTCTTTGTTNANACCTATGGGACCGGTAAAAAGACTGATG
CTGAAATCCNTGCCATCATCGATAANAACTTTGATTTACGTCCAGGTTGTTTAANGNGTG
ACCNCCAATTAACTCGNCCAATTTTCCAAAAAACNGCCNCTGGTGGNCNCTTTGGTAAAA
ANGANCCAGACNTTACCTGGGAAACCGNCAAAGAACTCAAGATGTAAGGATAGA
sequence update 2001. 3.22
Translated Amino Acid sequence 
VIFHTIYKMSSYLFTSESVTEGHPDKICDQVSDAVLDACLAQDPLSKVACETATKTGMVM
ILGEITTKANVDYQKVVREAVKKIGYDDSSKGFDYKTCNVLVAIEQQSPDIAQGVHIGKV
NPEDIGAGDQGHMFGYATNETPTLMPLTHFLASELVNKLTELRHNGTLAWARPDAKTQVT
VEYKKEGHKITPVRV---

---YHLNPSGRFVIGGPMGDXGLTGXXIIIDSYGGWGAXGGGAXSGKXATKXDRXGAYAA
KWIAKSLVAXGLADRCLVQVSXSIGXAXPLSVFVXTYGTGKKTDAEIXAIIDXNFDLRPG
CLXXDXQLTRPIFQKTAXGGXFGKXXPDXTWETXKELKM*g*
Translated Amino Acid sequence (All Frames) 
Frame A:
VIFHTIYKMSSYLFTSESVTEGHPDKICDQVSDAVLDACLAQDPLSKVACETATKTGMVM
ILGEITTKANVDYQKVVREAVKKIGYDDSSKGFDYKTCNVLVAIEQQSPDIAQGVHIGKV
NPEDIGAGDQGHMFGYATNETPTLMPLTHFLASELVNKLTELRHNGTLAWARPDAKTQVT
VEYKKEGHKITPVRV---

---YHLNPSGRFVIGGPMGDXGLTGXXIIIDSYGGWGAXGGGAXSGKXATKXDRXGAYAA
KWIAKSLVAXGLADRCLVQVSXSIGXAXPLSVFVXTYGTGKKTDAEIXAIIDXNFDLRPG
CLXXDXQLTRPIFQKTAXGGXFGKXXPDXTWETXKELKM*g*

Frame B:
*yftqyikchliyslvnpspkviqiksvikyqmlfsmlv*lkihyqkllvklqpklvwl*
f*vklppkptltikklfvkllkrlvmmthqkvlitklamf*lqlnnnhqillkvstlvkl
tqkilvlvikvtclvmpptkpql*chllisspln*stn*lnyvtmvp*pglvqmprlksl
lntkkkvtkslqsey---

---ti*ihqvvsslvaqwvixv*pvvxsslihmxvgvxxvvvlxlvxxplxsivxxltll
ngslnl*lplvlpivv*fkfxtllxxpxhsvslxxpmgpvkrlmlksxpssixtliyvqv
v*xvtxn*lxqfskkxplvxxlvkxxqtlpgkpxknsrckdr

Frame C:
nishni*nvilfih**irhrrssr*nl*ssirccsrclfssrsiikscl*ncnqnwygyd
fr*nyhqsqr*lskscs*sc*kdwl**likrf*lqnlqcfscn*ttitrycsrcphw*s*
prrywcw*srshvwlchqrnpnfnatysfprl*isqqin*itsqwylslgssrcqdsshc
*iqkrrsqnhssqs---

---pfesirsfrhwwpng*xwfnrx*xhh*fiwxlgcxrwwcfxw*xxh*xrsxwxlrc*
mdr*ifscxwscrslfsssfxlywxcqxtqclcxxlwdr*kd*c*npchhr*xl*ftsrl
fxx*ppinsxnfpknxxwwxlw*kxxrxylgnrqrtqdvri
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI333 (VSI333Q) /CSM/VS/VSI3-B/VSI333Q.Seq.d/                       1873   0.0
VFN896 (VFN896Q) /CSM/VF/VFN8-D/VFN896Q.Seq.d/                       1162   0.0
VFN766 (VFN766Q) /CSM/VF/VFN7-C/VFN766Q.Seq.d/                       1162   0.0
VFN576 (VFN576Q) /CSM/VF/VFN5-D/VFN576Q.Seq.d/                       1162   0.0
VFN463 (VFN463Q) /CSM/VF/VFN4-C/VFN463Q.Seq.d/                       1162   0.0
VFN346 (VFN346Q) /CSM/VF/VFN3-B/VFN346Q.Seq.d/                       1162   0.0
VFN312 (VFN312Q) /CSM/VF/VFN3-A/VFN312Q.Seq.d/                       1162   0.0
VFN165 (VFN165Q) /CSM/VF/VFN1-C/VFN165Q.Seq.d/                       1162   0.0
VFN128 (VFN128Q) /CSM/VF/VFN1-B/VFN128Q.Seq.d/                       1162   0.0
VFM886 (VFM886Q) /CSM/VF/VFM8-D/VFM886Q.Seq.d/                       1162   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268525) Dictyostelium discoideum vegetative cDNA clone:VS...  1162   0.0    1
(BJ417835) Dictyostelium discoideum cDNA clone:ddv28p24, 5' ...  1162   0.0    1
(BJ417793) Dictyostelium discoideum cDNA clone:ddv28g19, 5' ...  1162   0.0    1
(BJ417710) Dictyostelium discoideum cDNA clone:ddv28d17, 5' ...  1162   0.0    1
(BJ416896) Dictyostelium discoideum cDNA clone:ddv27n16, 5' ...  1162   0.0    1
(BJ416838) Dictyostelium discoideum cDNA clone:ddv27a17, 5' ...  1162   0.0    1
(BJ416814) Dictyostelium discoideum cDNA clone:ddv27l11, 5' ...  1162   0.0    1
(BJ416793) Dictyostelium discoideum cDNA clone:ddv27g07, 5' ...  1162   0.0    1
(BJ416725) Dictyostelium discoideum cDNA clone:ddv27h03, 5' ...  1162   0.0    1
(BJ416607) Dictyostelium discoideum cDNA clone:ddv26o23, 5' ...  1162   0.0    1
dna update 2009. 7.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54F07) RecName: Full=S-adenosylmethionine synthetase;         ...   379   e-104
S51671(S51671)methionine adenosyltransferase (EC 2.5.1.6) - Acan...   243   2e-91
(Q1H4X2) RecName: Full=S-adenosylmethionine synthetase;         ...   226   1e-86
CP000817_4064(CP000817|pid:none) Lysinibacillus sphaericus C3-41...   206   8e-79
CP001034_2296(CP001034|pid:none) Natranaerobius thermophilus JW/...   196   4e-77
(A1UFL0) RecName: Full=S-adenosylmethionine synthetase;         ...   195   2e-75
CP000687_1046(CP000687|pid:none) Actinobacillus pleuropneumoniae...   187   3e-73
AY324626_1(AY324626|pid:none) Amoeba proteus S-adenosylmethionin...   277   4e-73
(A3N186) RecName: Full=S-adenosylmethionine synthetase;         ...   187   2e-72
AP009247_658(AP009247|pid:none) Candidatus Vesicomyosocius okuta...   179   2e-72
protein update 2009. 3.24
PSORT 

 psg: 0.88  gvh: 0.51  alm: 0.42  top: 0.37  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 24.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	 12.0 %: vacuolar
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for VSI333 is cyt
5' end seq. ID VSI333F
5' end seq. 
>VSI333F.Seq
GTAATATTTCACACAATATATAAAATGTCATCTTATTTATTCACTAGTGAATCCGTCACC
GAAGGTCATCCAGATAAAATCTGTGATCAAGTATCAGATGCTGTTCTCGATGCTTGTTTA
GCTCAAGATCCATTATCAAAAGTTGCTTGTGAAACTGCAACCAAAACTGGTATGGTTATG
ATTTTAGGTGAAATTACCACCAAAGCCAACGTTGACTATCAAAAAGTTGTTCGTGAAGCT
GTTAAAAAGATTGGTTATGATGACTCATCAAAAGGTTTTGATTACAAAACTTGCAATGTT
TTAGTTGCAATTGAACAACAATCACCAGATATTGCTCAAGGTGTCCACATTGGTAAAGTT
AACCCAGAAGATATTGGTGCTGGTGATCAAGGTCACATGTTTGGTTATGCCACCAACGAA
ACCCCAACTTTAATGCCACTTACTCATTTCCTCGCCTCTGAATTAGTCAACAAATTAACT
GAATTACGTCACAATGGTACCTTAGCCTGGGCTCGTCCAGATGCCAAGACTCAAGTCACT
GTTGAATACAAAAAAGAAGGTCACAAAATCACTCCAGTCAGAGTAC----------
Length of 5' end seq. 586
3' end seq. ID VSI333Z
3' end seq. 
>VSI333Z.Seq
----------TACCATTTGAATCCATCAGGTCGTTTCGTCATTGGTGGCCCAATGGGTGA
TTNTGGTTTAACCGGTNGTAANATCATCATTGATTCATATGGNGGTTGGGGTGCNCNCGG
TGGTGGTGCTTTNTCTGGTAAANANGCCACTAAANTCGATCGTTNTGGNGCTTACGCTGC
TAAATGGATCGCTAAATCTTTAGTTGCCNCTGGTCTTGCCGATCGTTGTTTAGTTCAAGT
TTCANACTCTATTGGNGNTGCCAANCCACTCAGTGTCTTTGTTNANACCTATGGGACCGG
TAAAAAGACTGATGCTGAAATCCNTGCCATCATCGATAANAACTTTGATTTACGTCCAGG
TTGTTTAANGNGTGACCNCCAATTAACTCGNCCAATTTTCCAAAAAACNGCCNCTGGTGG
NCNCTTTGGTAAAAANGANCCAGACNTTACCTGGGAAACCGNCAAAGAACTCAAGATGTA
AGGATAGA
Length of 3' end seq. 478
Connected seq. ID VSI333P
Connected seq. 
>VSI333P.Seq
GTAATATTTCACACAATATATAAAATGTCATCTTATTTATTCACTAGTGAATCCGTCACC
GAAGGTCATCCAGATAAAATCTGTGATCAAGTATCAGATGCTGTTCTCGATGCTTGTTTA
GCTCAAGATCCATTATCAAAAGTTGCTTGTGAAACTGCAACCAAAACTGGTATGGTTATG
ATTTTAGGTGAAATTACCACCAAAGCCAACGTTGACTATCAAAAAGTTGTTCGTGAAGCT
GTTAAAAAGATTGGTTATGATGACTCATCAAAAGGTTTTGATTACAAAACTTGCAATGTT
TTAGTTGCAATTGAACAACAATCACCAGATATTGCTCAAGGTGTCCACATTGGTAAAGTT
AACCCAGAAGATATTGGTGCTGGTGATCAAGGTCACATGTTTGGTTATGCCACCAACGAA
ACCCCAACTTTAATGCCACTTACTCATTTCCTCGCCTCTGAATTAGTCAACAAATTAACT
GAATTACGTCACAATGGTACCTTAGCCTGGGCTCGTCCAGATGCCAAGACTCAAGTCACT
GTTGAATACAAAAAAGAAGGTCACAAAATCACTCCAGTCAGAGTAC----------TACC
ATTTGAATCCATCAGGTCGTTTCGTCATTGGTGGCCCAATGGGTGATTNTGGTTTAACCG
GTNGTAANATCATCATTGATTCATATGGNGGTTGGGGTGCNCNCGGTGGTGGTGCTTTNT
CTGGTAAANANGCCACTAAANTCGATCGTTNTGGNGCTTACGCTGCTAAATGGATCGCTA
AATCTTTAGTTGCCNCTGGTCTTGCCGATCGTTGTTTAGTTCAAGTTTCANACTCTATTG
GNGNTGCCAANCCACTCAGTGTCTTTGTTNANACCTATGGGACCGGTAAAAAGACTGATG
CTGAAATCCNTGCCATCATCGATAANAACTTTGATTTACGTCCAGGTTGTTTAANGNGTG
ACCNCCAATTAACTCGNCCAATTTTCCAAAAAACNGCCNCTGGTGGNCNCTTTGGTAAAA
ANGANCCAGACNTTACCTGGGAAACCGNCAAAGAACTCAAGATGTAAGGATAGA
Length of connected seq. 1064
Full length Seq ID -
Full length Seq. -
Length of full length seq. -