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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI331
Library VS
(Link to library)
Clone ID VSI331
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13726-1
Original site URL
Representative seq. ID VSI331E
(Link to Original site)
Representative DNA sequence 
>VSI331 (VSI331Q) /CSM/VS/VSI3-B/VSI331Q.Seq.d/
CTTTTACTTTTGTATTACAAAATGAGATTCATTTTAGTTTTAGTTTTAATTTTGGGCTTA
GTCTCATCATCATTTGGTATCAATGTTGACAAGAATGAAGTATCTCAAACCGAATTACAA
TCAGCTCGTGACTTTAGTTCATTCGCTATCGGCTTTGCAGAGGGGTATTGAAATTAGTTT
GACTGGTAATCTTCATAAATGTGTTGCTGCAGCCGAATCAAAGTTTCAGTGAATTCTCAA
ATTCCTTCCATTTAATTGATAGTGGTTTGAAACATAAATCATTAGGTGATGCAAAGAGTG
GTCTCCGTGATTTTGGTATTGGTTTAGTTGATTTGGTCAAAACCTACCAAAGATGTGGTG
TTGGAAAATTCATTAGTGAAATCTCTGCCATTTCAAAAGAAGTTACCAACGAAACTGGTA
TCATCAAACTCATTATTCATGAAGTCATCGATATTTTCCACAACTCCCACAGTTTAACTT
CCGATTTCAAATCCGCCATTTCAGATTGTGGTCGTGGTGATTACACTGGTTGTGGTGTTG
CAAGTGGAAAAATTGTCGGAATTTTAATGAGACAATAAATT
sequence update 2001. 3.25
Translated Amino Acid sequence 
ftfvlqneihfsfsfnfglsliiiwyqc*qe*sisnritiss*l*firyrlcrgvlklv*
LVIFINVLLQPNQSFSEFSNSFHLIDSGLKHKSLGDAKSGLRDFGIGLVDLVKTYQRCGV
GKFISEISAISKEVTNETGIIKLIIHEVIDIFHNSHSLTSDFKSAISDCGRGDYTGCGVA
SGKIVGILMRQ*i
Translated Amino Acid sequence (All Frames) 
Frame A:
llllyykmrfilvlvlilglvsssfginvdknevsqtelqsardfssfaigfaegy*n*f
dw*ss*mcccsrikvsvnsqipsi*livv*ninh*vmqrvvsvilvlv*liwskptkdvv
lenslvkslpfqkklptklvssnslfmkssifsttptv*lpisnppfqivvvvitlvvvl
qveklsef**dnk


Frame B:
fyfcitk*dsf*f*f*fwa*shhhlvsmltrmkylkpnynqlvtlvhslsalqrgieisl
tgnlhkcvaaaeskfq*ilkflpfn**wfet*iir*ckewsp*fwywfs*fgqnlpkmwc
wkih**nlchfkrsyqrnwyhqthys*shryfpqlpqfnfrfqirhfrlwsw*lhwlwcc
kwkncrnfnetin


Frame C:
ftfvlqneihfsfsfnfglsliiiwyqc*qe*sisnritiss*l*firyrlcrgvlklv*
LVIFINVLLQPNQSFSEFSNSFHLIDSGLKHKSLGDAKSGLRDFGIGLVDLVKTYQRCGV
GKFISEISAISKEVTNETGIIKLIIHEVIDIFHNSHSLTSDFKSAISDCGRGDYTGCGVA
SGKIVGILMRQ*i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI331 (VSI331Q) /CSM/VS/VSI3-B/VSI331Q.Seq.d/                       1152   0.0
VSH863 (VSH863Q) /CSM/VS/VSH8-C/VSH863Q.Seq.d/                       1120   0.0
VSD240 (VSD240Q) /CSM/VS/VSD2-B/VSD240Q.Seq.d/                       1120   0.0
SSI272 (SSI272Q) /CSM/SS/SSI2-C/SSI272Q.Seq.d/                       1120   0.0
VHJ638 (VHJ638Q) /CSM/VH/VHJ6-B/VHJ638Q.Seq.d/                       1112   0.0
VSI479 (VSI479Q) /CSM/VS/VSI4-D/VSI479Q.Seq.d/                       1110   0.0
VSF681 (VSF681Q) /CSM/VS/VSF6-D/VSF681Q.Seq.d/                       1090   0.0
VSI305 (VSI305Q) /CSM/VS/VSI3-A/VSI305Q.Seq.d/                       1072   0.0
VSF110 (VSF110Q) /CSM/VS/VSF1-A/VSF110Q.Seq.d/                       1066   0.0
SSF481 (SSF481Q) /CSM/SS/SSF4-D/SSF481Q.Seq.d/                       1049   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU263701) Dictyostelium discoideum vegetative cDNA clone:VS...   716   0.0    3
(C90651) Dictyostelium discoideum slug cDNA, clone SSI272.        716   0.0    3
(AU268761) Dictyostelium discoideum vegetative cDNA clone:VS...   706   0.0    3
(AU268760) Dictyostelium discoideum vegetative cDNA clone:VS...   700   0.0    3
(BJ423780) Dictyostelium discoideum cDNA clone:ddv50k10, 5' ...   688   0.0    3
(AU265613) Dictyostelium discoideum vegetative cDNA clone:VS...   716   0.0    3
(BJ442597) Dictyostelium discoideum cDNA clone:ddv50k10, 3' ...   634   0.0    4
(AU268479) Dictyostelium discoideum vegetative cDNA clone:VS...   716   0.0    3
(AU264723) Dictyostelium discoideum vegetative cDNA clone:VS...   642   0.0    6
(AU265612) Dictyostelium discoideum vegetative cDNA clone:VS...   716   0.0    3
dna update 2009. 7.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q23911) RecName: Full=Protein 29C; Flags: Precursor;                 236   1e-78
(O77257) RecName: Full=Protein gp17; Flags: Precursor;  &Y17042_...    96   5e-19
(Q54KD5) RecName: Full=P17/29C-like protein DDB_G0287399; Flags:...    93   6e-18
M16346_1(M16346|pid:none) D.discoideum protein 29C gene, 5' end.       58   7e-09
(O42643) RecName: Full=Pre-mRNA-splicing factor ATP-dependent RN...    33   5.3
AY809096_1(AY809096|pid:none) Schistosoma japonicum SJCHGC08862 ...    33   6.9
BA000012_5963(BA000012|pid:none) Mesorhizobium loti MAFF303099 D...    32   9.0
protein update 2009. 3.24
PSORT 

 psg: 0.92  gvh: 0.55  alm: 0.46  top: 0.47  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 20.0 %: extracellular, including cell wall
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VSI331 is cyt
5' end seq. ID VSI331F
5' end seq. 
>VSI331F.Seq
CTTTTACTTTTGTATTACAAAATGAGATTCATTTTAGTTTTAGTTTTAATTTTGGGCTTA
GTCTCATCATCATTTGGTATCAATGTTGACAAGAATGAAGTATCTCAAACCGAATTACAA
TCAGCTCGTGACTTTAGTTCATTCGCTATCGGCTTTGCAGAGGGTATTGAAATTAGTTTG
ACTGGTAATCTTCATAAATGTGTTGCTGCAGCCGAATCAAGTTTCAGTGAATTCTCAAAT
TCCTTCCATTTAATTGATAGTGGTTTGAAACATAAATCATTAGGTGATGCAAAGAGTGGT
CTCCGTGATTTTGGTATTGGTTTAGTTGATTTGGTCAAAACCTACCAAAGATGTGGTGTT
GGAAAATTCATTAGTGAAATCTCTGCCATTTCAAAAGAAGTTACCAACGAAACTGGTATC
ATCAAACTCATTATTCATGAAGTCATCGATATTTTCCACAACTCCCACAGTTTAACTTCC
GATTTCAAATCCGCCATTTCAGATTGTGGTCGTGGTGATT----------
Length of 5' end seq. 520
3' end seq. ID VSI331Z
3' end seq. 
>VSI331Z.Seq
----------TATCTCAAACCGAATTACAATCAGCTCGTGACTTTAGTTCATTCGCTATC
GGCTTTGCAGAGGGGTATTGAAATTAGTTTGACTGGTAATCTTCATAAATGTGTTGCTGC
AGCCGAATCAAAGTTTCAGTGAATTCTCAAATTCCTTCCATTTAATTGATAGTGGTTTGA
AACATAAATCATTAGGTGATGCAAAGAGTGGTCTCCGTGATTTTGGTATTGGTTTAGTTG
ATTTGGTCAAAACCTACCAAAGATGTGGTGTTGGAAAATTCATTAGTGAAATCTCTGCCA
TTTCAAAAGAAGTTACCAACGAAACTGGTATCATCAAACTCATTATTCATGAAGTCATCG
ATATTTTCCACAACTCCCACAGTTTAACTTCCGATTTCAAATCCGCCATTTCAGATTGTG
GTCGTGGTGATTACACTGGTTGTGGTGTTGCAAGTGGAAAAATTGTCGGAATTTTAATGA
GACAATAAATT
Length of 3' end seq. 481
Connected seq. ID VSI331P
Connected seq. 
>VSI331P.Seq
CTTTTACTTTTGTATTACAAAATGAGATTCATTTTAGTTTTAGTTTTAATTTTGGGCTTA
GTCTCATCATCATTTGGTATCAATGTTGACAAGAATGAAGTATCTCAAACCGAATTACAA
TCAGCTCGTGACTTTAGTTCATTCGCTATCGGCTTTGCAGAGGGTATTGAAATTAGTTTG
ACTGGTAATCTTCATAAATGTGTTGCTGCAGCCGAATCAAGTTTCAGTGAATTCTCAAAT
TCCTTCCATTTAATTGATAGTGGTTTGAAACATAAATCATTAGGTGATGCAAAGAGTGGT
CTCCGTGATTTTGGTATTGGTTTAGTTGATTTGGTCAAAACCTACCAAAGATGTGGTGTT
GGAAAATTCATTAGTGAAATCTCTGCCATTTCAAAAGAAGTTACCAACGAAACTGGTATC
ATCAAACTCATTATTCATGAAGTCATCGATATTTTCCACAACTCCCACAGTTTAACTTCC
GATTTCAAATCCGCCATTTCAGATTGTGGTCGTGGTGATT----------TATCTCAAAC
CGAATTACAATCAGCTCGTGACTTTAGTTCATTCGCTATCGGCTTTGCAGAGGGGTATTG
AAATTAGTTTGACTGGTAATCTTCATAAATGTGTTGCTGCAGCCGAATCAAAGTTTCAGT
GAATTCTCAAATTCCTTCCATTTAATTGATAGTGGTTTGAAACATAAATCATTAGGTGAT
GCAAAGAGTGGTCTCCGTGATTTTGGTATTGGTTTAGTTGATTTGGTCAAAACCTACCAA
AGATGTGGTGTTGGAAAATTCATTAGTGAAATCTCTGCCATTTCAAAAGAAGTTACCAAC
GAAACTGGTATCATCAAACTCATTATTCATGAAGTCATCGATATTTTCCACAACTCCCAC
AGTTTAACTTCCGATTTCAAATCCGCCATTTCAGATTGTGGTCGTGGTGATTACACTGGT
TGTGGTGTTGCAAGTGGAAAAATTGTCGGAATTTTAATGAGACAATAAATT
Length of connected seq. 1001
Full length Seq ID VSI331E
Full length Seq. 
>VSI331E.Seq
CTTTTACTTTTGTATTACAAAATGAGATTCATTTTAGTTTTAGTTTTAATTTTGGGCTTA
GTCTCATCATCATTTGGTATCAATGTTGACAAGAATGAAGTATCTCAAACCGAATTACAA
TCAGCTCGTGACTTTAGTTCATTCGCTATCGGCTTTGCAGAGGGGTATTGAAATTAGTTT
GACTGGTAATCTTCATAAATGTGTTGCTGCAGCCGAATCAAAGTTTCAGTGAATTCTCAA
ATTCCTTCCATTTAATTGATAGTGGTTTGAAACATAAATCATTAGGTGATGCAAAGAGTG
GTCTCCGTGATTTTGGTATTGGTTTAGTTGATTTGGTCAAAACCTACCAAAGATGTGGTG
TTGGAAAATTCATTAGTGAAATCTCTGCCATTTCAAAAGAAGTTACCAACGAAACTGGTA
TCATCAAACTCATTATTCATGAAGTCATCGATATTTTCCACAACTCCCACAGTTTAACTT
CCGATTTCAAATCCGCCATTTCAGATTGTGGTCGTGGTGATTACACTGGTTGTGGTGTTG
CAAGTGGAAAAATTGTCGGAATTTTAATGAGACAATAAATT
Length of full length seq. 581