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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI271
Library VS
(Link to library)
Clone ID VSI271
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16090-1
Original site URL
Representative seq. ID VSI271P
(Link to Original site)
Representative DNA sequence 
>VSI271 (VSI271Q) /CSM/VS/VSI2-C/VSI271Q.Seq.d/
AAGGCTGCTAGAGAACTCCTCGTTCTTGATGAAAAAGACCCAAAACGTATCTTTGAAGGT
TCAGCTCTCCTCCGTAGACTCCACAAATTAGGTGTCATGGAAGAATCAAAGAACAAACTC
GATTATATTCTTAATCTTAAAGTTCAAGATTTCATGGAAAGACGTCTCCAAACTTTAGTC
TTCAAAAATGGTCTTGCCAAATCAATCCATCACGCTCGTGTTTTAATCAAAGGTAGACAC
ATCCGTGTTGGTAAACAATTAGTCAACGTTCCATCTTTCCTCGTCCGTGTTGAATCCCAA
AAGCACTTAGGTTTAGCTTCAACCTCCCCATTAGCTGGTGGTCGTCCAGGTCGTCGTGCC
CGTAAAATGGCCAAGAACAACTCATCAAAAGGTGAAGAAGAAAACTAAATTAATAAAAAT
AACCATTATATTAGAGAGTAGTATATTGTAGTAAAAAAAAAAXXXXXXXXXXAAAGGCTG
CTAGAGAACTCCTCGTTCTTGATGAAAAAGACCCAAAACGTATCTTTGAAGGTTCAGCTC
TCCTCCGTAGACTCCACAAATTAGGTGTCATGGAAGAATCAAAGAACAAACTCGATTATA
TTCTTAATCTTAAAGTTCAAGATTTCATGGAAAGACGTCTCCAAACTTTAGTCTTCAAAA
ATGGTCTTGCCAAATCAATCCATCACGCTCGTGTTTTAATCAAAGGTAGACACATCCGTG
TTGGTAAACAATTAGTCAACGTTCCATCTTTCCTCGTCCGTGTTGAATCCCAAAAGCACT
TAGGTTTAGCTTCAACCTCCCCATTAGCTGGTGGTCGTCCAGGTCGTCGTGCCCGTAAAA
TGGCCAAGAACAACTCATCAAAAGGTGAAGAAGAAAACTAAATTAATAAAAATAACCATT
ATATAGAG
sequence update 2001. 3.22
Translated Amino Acid sequence 
gc*rtprs**krpktyl*rfsspp*tpqirchgrikeqtrlys*s*ssrfhgktspnfsl
qkwscqinpsrscfnqr*thpcw*tisqrsifprpc*ipkalrfsfnlpiswwssrsscp
*ngqeqlikr*rrkln**k*ply*RVVYCSKKK---

---KAARELLVLDEKDPKRIFEGSALLRRLHKLGVMEESKNKLDYILNLKVQDFMERRLQ
TLVFKNGLAKSIHHARVLIKGRHIRVGKQLVNVPSFLVRVESQKHLGLASTSPLAGGRPG
RRARKMAKNNSSKGEEEN*inknnhyie
Translated Amino Acid sequence (All Frames) 
Frame A:
kaarellvldekdpkrifegsallrrlhklgvmeesknkldyilnlkvqdfmerrlqtlv
fknglaksihharvlikgrhirvgkqlvnvpsflvrvesqkhlglastsplaggrpgrra
rkmaknnsskgeeen*inknnhyire*yivvkkk---

---kgc*rtprs**krpktyl*rfsspp*tpqirchgrikeqtrlys*s*ssrfhgktsp
nfslqkwscqinpsrscfnqr*thpcw*tisqrsifprpc*ipkalrfsfnlpiswwssr
sscp*ngqeqlikr*rrkln**k*plyr

Frame B:
rllenssflmkktqnvslkvqlssvdstn*vswknqrtnsiiflilkfkiswkdvskl*s
skmvlpnqsitlvf*skvdtsvlvnn*stfhlsssvlnpkst*v*lqpph*lvvvqvvvp
vkwprtthqkvkkktklikitiilessil**kk---

---KAARELLVLDEKDPKRIFEGSALLRRLHKLGVMEESKNKLDYILNLKVQDFMERRLQ
TLVFKNGLAKSIHHARVLIKGRHIRVGKQLVNVPSFLVRVESQKHLGLASTSPLAGGRPG
RRARKMAKNNSSKGEEEN*inknnhyie

Frame C:
gc*rtprs**krpktyl*rfsspp*tpqirchgrikeqtrlys*s*ssrfhgktspnfsl
qkwscqinpsrscfnqr*thpcw*tisqrsifprpc*ipkalrfsfnlpiswwssrsscp
*ngqeqlikr*rrkln**k*ply*RVVYCSKKK---

---rllenssflmkktqnvslkvqlssvdstn*vswknqrtnsiiflilkfkiswkdvsk
l*sskmvlpnqsitlvf*skvdtsvlvnn*stfhlsssvlnpkst*v*lqpph*lvvvqv
vvpvkwprtthqkvkkktklikitii*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI271 (VSI271Q) /CSM/VS/VSI2-C/VSI271Q.Seq.d/                        896   0.0
VSB144 (VSB144Q) /CSM/VS/VSB1-B/VSB144Q.Seq.d/                        896   0.0
FC-BO11 (FC-BO11Q) /CSM/FC/FC-BO/FC-BO11Q.Seq.d/                      896   0.0
FC-AS15 (FC-AS15Q) /CSM/FC/FC-AS/FC-AS15Q.Seq.d/                      896   0.0
VHO354 (VHO354Q) /CSM/VH/VHO3-C/VHO354Q.Seq.d/                        878   0.0
VSK290 (VSK290Q) /CSM/VS/VSK2-D/VSK290Q.Seq.d/                        872   0.0
VSH713 (VSH713Q) /CSM/VS/VSH7-A/VSH713Q.Seq.d/                        866   0.0
VSF164 (VSF164Q) /CSM/VS/VSF1-C/VSF164Q.Seq.d/                        864   0.0
VHJ170 (VHJ170Q) /CSM/VH/VHJ1-C/VHJ170Q.Seq.d/                        860   0.0
VSC206 (VSC206Q) /CSM/VS/VSC2-A/VSC206Q.Seq.d/                        858   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X06636) Dictyostelium rp1024 gene for ribosomal protein 1024.    896   0.0    1
(AU284883) Dictyostelium discoideum gamete cDNA clone:FC-BO1...   896   0.0    1
(AU284404) Dictyostelium discoideum gamete cDNA clone:FC-AS1...   896   0.0    1
(AU268425) Dictyostelium discoideum vegetative cDNA clone:VS...   896   0.0    1
(AU261847) Dictyostelium discoideum vegetative cDNA clone:VS...   896   0.0    1
(BJ426622) Dictyostelium discoideum cDNA clone:ddv59l13, 5' ...   878   0.0    1
(AU270948) Dictyostelium discoideum vegetative cDNA clone:VS...   872   0.0    1
(AU267855) Dictyostelium discoideum vegetative cDNA clone:VS...   866   0.0    1
(AU268426) Dictyostelium discoideum vegetative cDNA clone:VS...   864   0.0    1
(AU264807) Dictyostelium discoideum vegetative cDNA clone:VS...   864   0.0    1
dna update 2009. 7. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P14132) RecName: Full=40S ribosomal protein S9; AltName: Full=4...   217   4e-55
AP007167_255(AP007167|pid:none) Aspergillus oryzae RIB40 genomic...   168   2e-40
CU633445_35(CU633445|pid:none) Podospora anserina genomic DNA ch...   168   2e-40
AM270039_25(AM270039|pid:none) Aspergillus niger contig An02c045...   167   4e-40
AY857466_1(AY857466|pid:none) Suberites domuncula S9 mRNA, compl...   166   7e-40
DQ387872_8(DQ387872|pid:none) Neurospora crassa strain Adiopodou...   165   2e-39
AJ749993_1(AJ749993|pid:none) Catharanthus roseus mRNA for 40S r...   164   3e-39
AY392170_1(AY392170|pid:none) Scyliorhinus canicula ribosomal pr...   164   3e-39
AM920435_274(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   164   3e-39
(Q9FLF0) RecName: Full=40S ribosomal protein S9-2;  &AB010077_2(...   164   3e-39
protein update 2009. 3.24
PSORT 

 psg: 0.69  gvh: 0.27  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.27  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.50  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 88.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for VSI271 is cyt
5' end seq. ID VSI271F
5' end seq. 
>VSI271F.Seq
AAGGCTGCTAGAGAACTCCTCGTTCTTGATGAAAAAGACCCAAAACGTATCTTTGAAGGT
TCAGCTCTCCTCCGTAGACTCCACAAATTAGGTGTCATGGAAGAATCAAAGAACAAACTC
GATTATATTCTTAATCTTAAAGTTCAAGATTTCATGGAAAGACGTCTCCAAACTTTAGTC
TTCAAAAATGGTCTTGCCAAATCAATCCATCACGCTCGTGTTTTAATCAAAGGTAGACAC
ATCCGTGTTGGTAAACAATTAGTCAACGTTCCATCTTTCCTCGTCCGTGTTGAATCCCAA
AAGCACTTAGGTTTAGCTTCAACCTCCCCATTAGCTGGTGGTCGTCCAGGTCGTCGTGCC
CGTAAAATGGCCAAGAACAACTCATCAAAAGGTGAAGAAGAAAACTAAATTAATAAAAAT
AACCATTATATTAGAGAGTAGTATATTGTAGTAAAAAAAAAA----------
Length of 5' end seq. 462
3' end seq. ID VSI271Z
3' end seq. 
>VSI271Z.Seq
----------AAAGGCTGCTAGAGAACTCCTCGTTCTTGATGAAAAAGACCCAAAACGTA
TCTTTGAAGGTTCAGCTCTCCTCCGTAGACTCCACAAATTAGGTGTCATGGAAGAATCAA
AGAACAAACTCGATTATATTCTTAATCTTAAAGTTCAAGATTTCATGGAAAGACGTCTCC
AAACTTTAGTCTTCAAAAATGGTCTTGCCAAATCAATCCATCACGCTCGTGTTTTAATCA
AAGGTAGACACATCCGTGTTGGTAAACAATTAGTCAACGTTCCATCTTTCCTCGTCCGTG
TTGAATCCCAAAAGCACTTAGGTTTAGCTTCAACCTCCCCATTAGCTGGTGGTCGTCCAG
GTCGTCGTGCCCGTAAAATGGCCAAGAACAACTCATCAAAAGGTGAAGAAGAAAACTAAA
TTAATAAAAATAACCATTATATAGAG
Length of 3' end seq. 436
Connected seq. ID VSI271P
Connected seq. 
>VSI271P.Seq
AAGGCTGCTAGAGAACTCCTCGTTCTTGATGAAAAAGACCCAAAACGTATCTTTGAAGGT
TCAGCTCTCCTCCGTAGACTCCACAAATTAGGTGTCATGGAAGAATCAAAGAACAAACTC
GATTATATTCTTAATCTTAAAGTTCAAGATTTCATGGAAAGACGTCTCCAAACTTTAGTC
TTCAAAAATGGTCTTGCCAAATCAATCCATCACGCTCGTGTTTTAATCAAAGGTAGACAC
ATCCGTGTTGGTAAACAATTAGTCAACGTTCCATCTTTCCTCGTCCGTGTTGAATCCCAA
AAGCACTTAGGTTTAGCTTCAACCTCCCCATTAGCTGGTGGTCGTCCAGGTCGTCGTGCC
CGTAAAATGGCCAAGAACAACTCATCAAAAGGTGAAGAAGAAAACTAAATTAATAAAAAT
AACCATTATATTAGAGAGTAGTATATTGTAGTAAAAAAAAAA----------AAAGGCTG
CTAGAGAACTCCTCGTTCTTGATGAAAAAGACCCAAAACGTATCTTTGAAGGTTCAGCTC
TCCTCCGTAGACTCCACAAATTAGGTGTCATGGAAGAATCAAAGAACAAACTCGATTATA
TTCTTAATCTTAAAGTTCAAGATTTCATGGAAAGACGTCTCCAAACTTTAGTCTTCAAAA
ATGGTCTTGCCAAATCAATCCATCACGCTCGTGTTTTAATCAAAGGTAGACACATCCGTG
TTGGTAAACAATTAGTCAACGTTCCATCTTTCCTCGTCCGTGTTGAATCCCAAAAGCACT
TAGGTTTAGCTTCAACCTCCCCATTAGCTGGTGGTCGTCCAGGTCGTCGTGCCCGTAAAA
TGGCCAAGAACAACTCATCAAAAGGTGAAGAAGAAAACTAAATTAATAAAAATAACCATT
ATATAGAG
Length of connected seq. 898
Full length Seq ID -
Full length Seq. -
Length of full length seq. -