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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI263
Library VS
(Link to library)
Clone ID VSI263
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12167-1
Original site URL
Representative seq. ID VSI263P
(Link to Original site)
Representative DNA sequence 
>VSI263 (VSI263Q) /CSM/VS/VSI2-C/VSI263Q.Seq.d/
CACATAGCATATAAATTAACAACAATGGAAGGAGAACAATTACAAACTGCACCATACAAC
CCAAGATTCCCACAACAAAACCAAACTAAACATTGTTGGGCTAACTACGTTGATTACTAT
GGTTGTGTCAAACATTACAATGGTGACAACTCAAAATGTCAAACTTTTTTCAACTCAATG
AACTCATTATGTCCAGCTGCCTGGATTTCAGAATGGGATGAACAAAAAGCCGCTGATCTC
TTCCCATCAGACAGAGTTTAAATATATAATATAAATAATTAATAGTAGTATCAATAATTT
ATTCAAAAAAAAAGAAAAAAAAAAAGGAXXXXXXXXXXANNATTTATTCNAAAAAAAAGA
AAAAAAAAAAAGAACATAATGATATAAATAAAAAAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
t*hin*qqwkennyklhhttqdshnktklnivglttlitmvvsnitmvttqnvklfstq*
thyvqlpgfqngmnkkplisshqtefkyii*IINSSINNLFKKKEKKKG---

---XIYSKKKKKKKEHNDINKK
Translated Amino Acid sequence (All Frames) 
Frame A:
hiayklttmegeqlqtapynprfpqqnqtkhcwanyvdyygcvkhyngdnskcqtffnsm
nslcpaawisewdeqkaadlfpsdrv*iyninn***yq*fiqkkrkkkr---

---XIYSKKKKKKKEHNDINKK

Frame B:
t*hin*qqwkennyklhhttqdshnktklnivglttlitmvvsnitmvttqnvklfstq*
thyvqlpgfqngmnkkplisshqtefkyii*IINSSINNLFKKKEKKKG---

---xfixkkrkkkknimi*ikk

Frame C:
hsi*innngrrtitnctiqpkipttkpn*tllg*lr*llwlcqtlqw*qlkmsnffqlne
limsscldfrmg*tksr*slpirqslni*yk*livvsiiyskkkkkkk---

---xlfxkkekkkrt**yk*kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI263 (VSI263Q) /CSM/VS/VSI2-C/VSI263Q.Seq.d/                        603   e-172
VSH265 (VSH265Q) /CSM/VS/VSH2-C/VSH265Q.Seq.d/                        603   e-172
VSD559 (VSD559Q) /CSM/VS/VSD5-C/VSD559Q.Seq.d/                        603   e-172
VSA196 (VSA196Q) /CSM/VS/VSA1-D/VSA196Q.Seq.d/                        603   e-172
VHK558 (VHK558Q) /CSM/VH/VHK5-C/VHK558Q.Seq.d/                        603   e-172
SSF773 (SSF773Q) /CSM/SS/SSF7-D/SSF773Q.Seq.d/                        589   e-167
VSH131 (VSH131Q) /CSM/VS/VSH1-B/VSH131Q.Seq.d/                        587   e-167
SSE738 (SSE738Q) /CSM/SS/SSE7-B/SSE738Q.Seq.d/                        583   e-166
VSJ228 (VSJ228Q) /CSM/VS/VSJ2-B/VSJ228Q.Seq.d/                        565   e-160
VSF762 (VSF762Q) /CSM/VS/VSF7-C/VSF762Q.Seq.d/                        565   e-160
own update 2003. 1.10
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU261346) Dictyostelium discoideum vegetative cDNA clone:VS...   603   e-171  2
(AU268413) Dictyostelium discoideum vegetative cDNA clone:VS...   603   e-168  1
(AU267162) Dictyostelium discoideum vegetative cDNA clone:VS...   603   e-168  1
(AU264240) Dictyostelium discoideum vegetative cDNA clone:VS...   603   e-168  1
(BJ424463) Dictyostelium discoideum cDNA clone:ddv52c15, 5' ...   603   e-168  1
(C93078) Dictyostelium discoideum slug cDNA, clone SSF773.        589   e-167  2
(C84718) Dictyostelium discoideum slug cDNA, clone SSE738.        583   e-165  2
(AU266949) Dictyostelium discoideum vegetative cDNA clone:VS...   474   e-162  2
(AU267163) Dictyostelium discoideum vegetative cDNA clone:VS...   579   e-161  1
(AU269606) Dictyostelium discoideum vegetative cDNA clone:VS...   565   e-157  1
dna update 2009. 7. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q01519) RecName: Full=Cytochrome c oxidase polypeptide VIb;    ...    89   4e-17
AM920427_744(AM920427|pid:none) Penicillium chrysogenum Wisconsi...    87   1e-16
AM269997_1(AM269997|pid:none) Aspergillus niger contig An02c0020...    87   2e-16
CR382131_683(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...    86   4e-16
CR382126_746(CR382126|pid:none) Kluyveromyces lactis strain NRRL...    86   4e-16
CP000497_298(CP000497|pid:none) Pichia stipitis CBS 6054 chromos...    84   2e-15
AE017346_138(AE017346|pid:none) Cryptococcus neoformans var. neo...    84   2e-15
(P14854) RecName: Full=Cytochrome c oxidase subunit VIb isoform ...    82   5e-15
(Q5RCT0) RecName: Full=Cytochrome c oxidase subunit VIb isoform ...    82   5e-15
AE014298_3053(AE014298|pid:none) Drosophila melanogaster chromos...    80   2e-14
protein update 2009. 3.24
PSORT 

 psg: 0.22  gvh: -0.10  alm: 0.78  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.80  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 24.0 %: cytoplasmic
	  4.0 %: plasma membrane

>> prediction for VSI263 is nuc
5' end seq. ID VSI263F
5' end seq. 
>VSI263F.Seq
CACATAGCATATAAATTAACAACAATGGAAGGAGAACAATTACAAACTGCACCATACAAC
CCAAGATTCCCACAACAAAACCAAACTAAACATTGTTGGGCTAACTACGTTGATTACTAT
GGTTGTGTCAAACATTACAATGGTGACAACTCAAAATGTCAAACTTTTTTCAACTCAATG
AACTCATTATGTCCAGCTGCCTGGATTTCAGAATGGGATGAACAAAAAGCCGCTGATCTC
TTCCCATCAGACAGAGTTTAAATATATAATATAAATAATTAATAGTAGTATCAATAATTT
ATTCAAAAAAAAAGAAAAAAAAAAAGGA----------
Length of 5' end seq. 328
3' end seq. ID VSI263Z
3' end seq. 
>VSI263Z.Seq
----------ANNATTTATTCNAAAAAAAAGAAAAAAAAAAAAGAACATAATGATATAAA
TAAAAAAAA
Length of 3' end seq. 59
Connected seq. ID VSI263P
Connected seq. 
>VSI263P.Seq
CACATAGCATATAAATTAACAACAATGGAAGGAGAACAATTACAAACTGCACCATACAAC
CCAAGATTCCCACAACAAAACCAAACTAAACATTGTTGGGCTAACTACGTTGATTACTAT
GGTTGTGTCAAACATTACAATGGTGACAACTCAAAATGTCAAACTTTTTTCAACTCAATG
AACTCATTATGTCCAGCTGCCTGGATTTCAGAATGGGATGAACAAAAAGCCGCTGATCTC
TTCCCATCAGACAGAGTTTAAATATATAATATAAATAATTAATAGTAGTATCAATAATTT
ATTCAAAAAAAAAGAAAAAAAAAAAGGA----------ANNATTTATTCNAAAAAAAAGA
AAAAAAAAAAAGAACATAATGATATAAATAAAAAAAA
Length of connected seq. 387
Full length Seq ID -
Full length Seq. -
Length of full length seq. -