>VSI256 (VSI256Q) /CSM/VS/VSI2-C/VSI256Q.Seq.d/
CCGTGAAAAGAATTATTCAGTAAAGCCAACTATAGATACTTTACATTCCGCTGTATTAAA
TGGTTATACGGAAATAATTAAATATCTTTGTGAAACGTTACCACCGATCTCGTCATTTCC
ACCACCATTAAGAGTACCTCAATTACAACAAGTATTAATGATGTTAATGACATGTGAAAA
AAACAATGATTTTCAATCATTTACCACTATTATACAATCTAAATCTATAACGTTTTTTAG
ATTTGTAGAATCTAAATCTGTTTTAGAATATAAGTTACTTGAAAATAAAGTAAATTTTAA
ATCTAAATTTTTAAATCACCTTTTTAAAGAGAAATTAATAGATAGTAAAGCTATATTTAA
AAGGTATTGTAAAATTGATTTAAATTTATTTTTATTATTTAAATATAATCAAGATTGGAT
TAATCAAGAATACTTTTTTCAATTAATTCAAGATTCAAATATTCAAATTTTTAATAATAT
TATACTTGATGAATTGTTTTTATCAACTTTTTTAATAACTGATAAATTAAAAGATAAATT
TATAATXXXXXXXXXX

REKNYSVKPTIDTLHSAVLNGYTEIIKYLCETLPPISSFPPPLRVPQLQQVLMMLMTCEK
NNDFQSFTTIIQSKSITFFRFVESKSVLEYKLLENKVNFKSKFLNHLFKEKLIDSKAIFK
RYCKIDLNLFLLFKYNQDWINQEYFFQLIQDSNIQIFNNIILDELFLSTFLITDKLKDKF
I---

Frame A:
p*kelfskanyryftfrcikwlygnn*isl*nvttdlvisttikstsittsindvndm*k
kq*fsiiyhyyti*iynvf*icri*icfri*vt*k*skf*i*ifkspf*reinr**syi*
kvl*n*fkfifii*i*srld*srilfsinsrfkysnf**yyt**ivfinffnn**ikr*i
y---


Frame B:
REKNYSVKPTIDTLHSAVLNGYTEIIKYLCETLPPISSFPPPLRVPQLQQVLMMLMTCEK
NNDFQSFTTIIQSKSITFFRFVESKSVLEYKLLENKVNFKSKFLNHLFKEKLIDSKAIFK
RYCKIDLNLFLLFKYNQDWINQEYFFQLIQDSNIQIFNNIILDELFLSTFLITDKLKDKF
I---


Frame C:
vkriiq*sql*ilyiply*mvirk*lnifvkryhrsrhfhhh*eylnynky**c**hvkk
tmifnhlpllynlnl*rfldl*nlnlf*nisylkik*ilnlnf*itflkrn**ivklylk
givkli*iyfyylniikiglikntffn*fkiqifkfliilylmncfyqlf**lin*kinl
*---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI256 (VSI256Q) /CSM/VS/VSI2-C/VSI256Q.Seq.d/                        513   e-145
SLE674 (SLE674Q) /CSM/SL/SLE6-D/SLE674Q.Seq.d/                         40   0.035
SHD179 (SHD179Q) /CSM/SH/SHD1-D/SHD179Q.Seq.d/                         36   0.55
VSJ823 (VSJ823Q) /CSM/VS/VSJ8-A/VSJ823Q.Seq.d/                         34   2.2
VSJ703 (VSJ703Q) /CSM/VS/VSJ7-A/VSJ703Q.Seq.d/                         34   2.2
VSJ442 (VSJ442Q) /CSM/VS/VSJ4-B/VSJ442Q.Seq.d/                         34   2.2
VSH337 (VSH337Q) /CSM/VS/VSH3-B/VSH337Q.Seq.d/                         34   2.2
VSH135 (VSH135Q) /CSM/VS/VSH1-B/VSH135Q.Seq.d/                         34   2.2
VSG583 (VSG583Q) /CSM/VS/VSG5-D/VSG583Q.Seq.d/                         34   2.2
VSD155 (VSD155Q) /CSM/VS/VSD1-C/VSD155Q.Seq.d/                         34   2.2

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268403) Dictyostelium discoideum vegetative cDNA clone:VS...   349   0.0    3
(AC116030) Dictyostelium discoideum chromosome 2 map 2090851...   343   0.0    3
(EK324311) 1095467000945 Global-Ocean-Sampling_GS-31-01-01-1...    42   0.015  2
(CU104756) Zebrafish DNA sequence from clone DKEY-232D5 in l...    50   0.091  1
(EJ435487) 1093015278551 Global-Ocean-Sampling_GS-28-01-01-1...    36   0.24   3
(DE154799) Bombyx mori genomic DNA, Fosmid clone:RO0297-E06_R.     36   0.27   2
(AF250284) Amsacta moorei entomopoxvirus, complete genome.         36   0.33   10
(DQ280166) Muntiacus muntjak vaginalis chromosome 1 clone 05...    48   0.36   1
(EK229169) 1095460172529 Global-Ocean-Sampling_GS-31-01-01-1...    48   0.36   1
(DH191776) Oryzias latipes Fosmid clone:GOLWFno477_k08, forw...    48   0.36   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116030_2(AC116030|pid:none) Dictyostelium discoideum chromosom...   322   4e-87
AE010300_3327(AE010300|pid:none) Leptospira interrogans serovar ...    39   0.087
CP000487_1531(CP000487|pid:none) Campylobacter fetus subsp. fetu...    38   0.19
(Q9MUP3) RecName: Full=Uncharacterized protein ycf81;          S...    36   0.74
(Q2EEX5) RecName: Full=Uncharacterized membrane protein ycf78; A...    35   0.97
CP000770_186(CP000770|pid:none) Candidatus Sulcia muelleri GWSS,...    35   1.6
CP000082_667(CP000082|pid:none) Psychrobacter arcticus 273-4, co...    35   1.6
S10439(S10439) DNA-directed RNA polymerase (EC 2.7.7.6) beta' ch...    35   1.6
AL844509_388(AL844509|pid:none) Plasmodium falciparum 3D7 chromo...    35   1.6
(P21422) RecName: Full=DNA-directed RNA polymerase subunit beta'...    35   1.6

 psg: 0.72  gvh: 0.57  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VSI256 is cyt

>VSI256F.Seq
CCGTGAAAAGAATTATTCAGTAAAGCCAACTATAGATACTTTACATTCCGCTGTATTAAA
TGGTTATACGGAAATAATTAAATATCTTTGTGAAACGTTACCACCGATCTCGTCATTTCC
ACCACCATTAAGAGTACCTCAATTACAACAAGTATTAATGATGTTAATGACATGTGAAAA
AAACAATGATTTTCAATCATTTACCACTATTATACAATCTAAATCTATAACGTTTTTTAG
ATTTGTAGAATCTAAATCTGTTTTAGAATATAAGTTACTTGAAAATAAAGTAAATTTTAA
ATCTAAATTTTTAAATCACCTTTTTAAAGAGAAATTAATAGATAGTAAAGCTATATTTAA
AAGGTATTGTAAAATTGATTTAAATTTATTTTTATTATTTAAATATAATCAAGATTGGAT
TAATCAAGAATACTTTTTTCAATTAATTCAAGATTCAAATATTCAAATTTTTAATAATAT
TATACTTGATGAATTGTTTTTATCAACTTTTTTAATAACTGATAAATTAAAAGATAAATT
TATAAT----------