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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI255
Library VS
(Link to library)
Clone ID VSI255
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10109-1
Original site URL
Representative seq. ID VSI255P
(Link to Original site)
Representative DNA sequence 
>VSI255 (VSI255Q) /CSM/VS/VSI2-C/VSI255Q.Seq.d/
TCCACGCGTGCCGAGTCAAATAAAGATGAAGTTTAATAATGCAGGTGCACAAGGTAGAGA
TAGAGGTTACATCTATTCTGAACCAGAGTTTGGAAGAACAAGACAAGTGTTTTATTAGAA
TTTTCAAATTATCTCATTTAAAATAAAATTTTAAAAAGATCATTTCAATTAAAAAAAAAA
XXXXXXXXXXAGCAAATAAAGATGAAGTTTAATAATGCAGGTGCACAAGGTAGAGATAGA
GGTTACATCTATTCTGAACCAGAGTTTGGAAGAACAAGACAAGTGTTTTATTAGAATTTT
CAAATTTCTCATTTAAAATAAATTTTAAAAAG
sequence update 2001. 3.22
Translated Amino Acid sequence 
straesnkdev**crctr*r*rlhlf*trvwknktsvllefsnyli*NKILKRSFQLKKK
---

---QIKMKFNNAGAQGRDRGYIYSEPEFGRTRQVFY*nfqishlk*ilk
Translated Amino Acid sequence (All Frames) 
Frame A:
straesnkdev**crctr*r*rlhlf*trvwknktsvllefsnyli*NKILKRSFQLKKK
---

---sk*r*slimqvhkveievtsilnqsleeqdkcfirifkfli*nkf*k

Frame B:
prvpsqikmkfnnagaqgrdrgyiysepefgrtrqvfy*nfqiisfkikf*kdhfn*kk-
--

---ankdev**crctr*r*rlhlf*trvwknktsvllefsnfsfkinfkk

Frame C:
hacrvk*r*slimqvhkveievtsilnqsleeqdkcfirifklshlk*nfkkiisikkk-
--

---QIKMKFNNAGAQGRDRGYIYSEPEFGRTRQVFY*nfqishlk*ilk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI255 (VSI255Q) /CSM/VS/VSI2-C/VSI255Q.Seq.d/                        337   6e-92
VSK376 (VSK376Q) /CSM/VS/VSK3-D/VSK376Q.Seq.d/                        305   2e-82
VSK213 (VSK213Q) /CSM/VS/VSK2-A/VSK213Q.Seq.d/                        305   2e-82
VSJ884 (VSJ884Q) /CSM/VS/VSJ8-D/VSJ884Q.Seq.d/                        305   2e-82
VSH545 (VSH545Q) /CSM/VS/VSH5-B/VSH545Q.Seq.d/                        305   2e-82
VSH284 (VSH284Q) /CSM/VS/VSH2-D/VSH284Q.Seq.d/                        305   2e-82
VSG737 (VSG737Q) /CSM/VS/VSG7-B/VSG737Q.Seq.d/                        305   2e-82
VSG315 (VSG315Q) /CSM/VS/VSG3-A/VSG315Q.Seq.d/                        305   2e-82
VSF264 (VSF264Q) /CSM/VS/VSF2-C/VSF264Q.Seq.d/                        305   2e-82
VSA324 (VSA324Q) /CSM/VS/VSA3-A/VSA324Q.Seq.d/                        305   2e-82
own update 2001.11.21
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268402) Dictyostelium discoideum vegetative cDNA clone:VS...   281   8e-72  1
(AU271092) Dictyostelium discoideum vegetative cDNA clone:VS...   264   2e-66  1
(AU270815) Dictyostelium discoideum vegetative cDNA clone:VS...   236   2e-63  2
(AU271644) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   230   2e-60  2
(AU267197) Dictyostelium discoideum vegetative cDNA clone:VS...   230   2e-60  2
(AU271645) Dictyostelium discoideum gamete cDNA clone:FC-IC0...   230   2e-60  2
(AU267604) Dictyostelium discoideum vegetative cDNA clone:VS...   230   2e-60  2
(AU267198) Dictyostelium discoideum vegetative cDNA clone:VS...   230   2e-60  2
(AU261422) Dictyostelium discoideum vegetative cDNA clone:VS...   230   2e-60  2
(AU266296) Dictyostelium discoideum vegetative cDNA clone:VS...   230   2e-60  2
dna update 2009. 7. 6
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3019103
Number of Hits to DB: 365,914,215
protein update 2009. 3.24
PSORT 

 psg: 0.45  gvh: 0.32  alm: 0.71  top: 0.53  tms: 0.00  mit: 0.44  mip: 0.02
 nuc: 0.12  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for VSI255 is nuc
5' end seq. ID VSI255F
5' end seq. 
>VSI255F.Seq
TCCACGCGTGCCGAGTCAAATAAAGATGAAGTTTAATAATGCAGGTGCACAAGGTAGAGA
TAGAGGTTACATCTATTCTGAACCAGAGTTTGGAAGAACAAGACAAGTGTTTTATTAGAA
TTTTCAAATTATCTCATTTAAAATAAAATTTTAAAAAGATCATTTCAATTAAAAAAAAAA
----------
Length of 5' end seq. 180
3' end seq. ID VSI255Z
3' end seq. 
>VSI255Z.Seq
----------AGCAAATAAAGATGAAGTTTAATAATGCAGGTGCACAAGGTAGAGATAGA
GGTTACATCTATTCTGAACCAGAGTTTGGAAGAACAAGACAAGTGTTTTATTAGAATTTT
CAAATTTCTCATTTAAAATAAATTTTAAAAAG
Length of 3' end seq. 142
Connected seq. ID VSI255P
Connected seq. 
>VSI255P.Seq
TCCACGCGTGCCGAGTCAAATAAAGATGAAGTTTAATAATGCAGGTGCACAAGGTAGAGA
TAGAGGTTACATCTATTCTGAACCAGAGTTTGGAAGAACAAGACAAGTGTTTTATTAGAA
TTTTCAAATTATCTCATTTAAAATAAAATTTTAAAAAGATCATTTCAATTAAAAAAAAAA
----------AGCAAATAAAGATGAAGTTTAATAATGCAGGTGCACAAGGTAGAGATAGA
GGTTACATCTATTCTGAACCAGAGTTTGGAAGAACAAGACAAGTGTTTTATTAGAATTTT
CAAATTTCTCATTTAAAATAAATTTTAAAAAG
Length of connected seq. 322
Full length Seq ID -
Full length Seq. -
Length of full length seq. -