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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI252
Library VS
(Link to library)
Clone ID VSI252
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10224-1
Original site URL
Representative seq. ID VSI252P
(Link to Original site)
Representative DNA sequence 
>VSI252 (VSI252Q) /CSM/VS/VSI2-C/VSI252Q.Seq.d/
CTTGACAAAATAATAAATGATATTAAAAAAGAGGCTAACGACTCTGGTGTTACTTTAGCT
CCACTTTCTGTTCCAAAACCTAAACTAGAGGAACTCTCAGAGCAACAAAAAATTATACTT
GCCGAATACATTGCAGAAGTTGGTTTACAGAATATAACAGCGATCACTCTCTCAAAAAAA
TTGAATATTACCGTAGAAAAAGCTAAAAATTATATAAAAAATTCTAACAGACTTGGCAGA
ACAAATAATTTAAAAACTATTGGTATTCTTCAAGAAGAGGTTTCATCTATGGAGGCTAAA
TCTATGACTTGGTAAAGATTTAGGTATAGAACTTGGTTCATTATTTTTTCTAAAAAAAAT
AAAACACTAAAATTAAAATATGAAAATAAAATAAAATAAAGCTAATCATTTAATAGTGAT
TAACAAACCCTCATTGTACGTAAAATTAAAAAAAAAAXXXXXXXXXXCTTGACAAAATAA
TAAATGATATTAAAAAAGAGGCTAACGACTCTGGTGTTACTTTAGCTCCACTTTCTGTTC
CAAAACCTAAACTAGAGGAACTCTCAGAGCAACAAAAAATTATACTTGCCGAATACATTG
CAGAAGTTGGTTTACAGAATATAACAGCGATCACTCTCTCAAAAAAATTGAATATTACCG
TAGAAAAAGCTAAAAATTATATAAAAAATTCTAACAGACTTGGCAGAACAAATAATTTAA
AAACTATTGGTATTCTTCAAGAAGAGGTTTCATCTATGGAGGCTAAATCTATGACTTGGT
AAAGATTTAGGTATAGAACTTGGTTCATTATTTTTTCTAAAAAAAATAAAACACTAAAAT
TAAAATATGAAAATAAAATAAAATAAAGCTAATCTTTAATAGNATTAACAAACCC
sequence update 2001. 3.22
Translated Amino Acid sequence 
ltk**milkkrlttlvll*lhflfqnln*rnsqsnkklylpntlqklvyri*qrslsqkn
*ilp*kklkii*kiltdlaeqii*kllvffkkrfhlwrlnl*lgkdlgielgslfflkki
kh*n*nmkik*NKANHLIVINKPSLYVKLKKK---

---LDKIINDIKKEANDSGVTLAPLSVPKPKLEELSEQQKIILAEYIAEVGLQNITAITL
SKKLNITVEKAKNYIKNSNRLGRTNNLKTIGILQEEVSSMEAKSMTW*rfryrtwfiifs
kknktlklkyenkik*s*slixltn
Translated Amino Acid sequence (All Frames) 
Frame A:
ldkiindikkeandsgvtlaplsvpkpkleelseqqkiilaeyiaevglqnitaitlskk
lnitvekaknyiknsnrlgrtnnlktigilqeevssmeaksmtw*rfryrtwfiifskkn
ktlklkyenkik*s*sfnsd*qtlivrkikkk---

---LDKIINDIKKEANDSGVTLAPLSVPKPKLEELSEQQKIILAEYIAEVGLQNITAITL
SKKLNITVEKAKNYIKNSNRLGRTNNLKTIGILQEEVSSMEAKSMTW*rfryrtwfiifs
kknktlklkyenkik*s*slixltn

Frame B:
ltk**milkkrlttlvll*lhflfqnln*rnsqsnkklylpntlqklvyri*qrslsqkn
*ilp*kklkii*kiltdlaeqii*kllvffkkrfhlwrlnl*lgkdlgielgslfflkki
kh*n*nmkik*NKANHLIVINKPSLYVKLKKK---

---ltk**milkkrlttlvll*lhflfqnln*rnsqsnkklylpntlqklvyri*qrsls
qkn*ilp*kklkii*kiltdlaeqii*kllvffkkrfhlwrlnl*lgkdlgielgslffl
kkikh*n*nmkik*nkanl**x*qt

Frame C:
*qnnk*y*krg*rlwcyfsstfcskt*trgtlratknytcrihcrswfteynsdhslkki
eyyrrks*klykkf*qtwqnk*fknywyssrrgfiygg*iydlvki*v*nlvhyff*kk*
ntkiki*k*nkiklii***ltnphct*n*kk---

---*qnnk*y*krg*rlwcyfsstfcskt*trgtlratknytcrihcrswfteynsdhsl
kkieyyrrks*klykkf*qtwqnk*fknywyssrrgfiygg*iydlvki*v*nlvhyff*
kk*ntkiki*k*nkiklifnxinkp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI252 (VSI252Q) /CSM/VS/VSI2-C/VSI252Q.Seq.d/                        347   2e-94
VSG453 (VSG453Q) /CSM/VS/VSG4-C/VSG453Q.Seq.d/                        347   2e-94
SLE177 (SLE177Q) /CSM/SL/SLE1-D/SLE177Q.Seq.d/                        276   6e-73
AHE844 (AHE844Q) /CSM/AH/AHE8-B/AHE844Q.Seq.d/                         38   0.23
AHE874 (AHE874Q) /CSM/AH/AHE8-D/AHE874Q.Seq.d/                         38   0.23
VHP767 (VHP767Q) /CSM/VH/VHP7-C/VHP767Q.Seq.d/                         38   0.23
VHP436 (VHP436Q) /CSM/VH/VHP4-B/VHP436Q.Seq.d/                         38   0.23
VHO880 (VHO880Q) /CSM/VH/VHO8-D/VHO880Q.Seq.d/                         38   0.23
VHO643 (VHO643Q) /CSM/VH/VHO6-B/VHO643Q.Seq.d/                         38   0.23
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268395) Dictyostelium discoideum vegetative cDNA clone:VS...   343   e-178  3
(AU266503) Dictyostelium discoideum vegetative cDNA clone:VS...   343   e-177  3
(AU268396) Dictyostelium discoideum vegetative cDNA clone:VS...   343   e-165  3
(AU266504) Dictyostelium discoideum vegetative cDNA clone:VS...   299   e-144  2
(AU061421) Dictyostelium discoideum slug cDNA, clone SLE177.      272   e-140  3
(EI298056) GM_WBc0023A02.r GM_WBc Glycine max genomic clone ...    40   0.002  2
(X95601) M.hominis lmp3 and lmp4 genes.                            42   0.017  2
(CU618341) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    38   0.026  6
(AX344573) Sequence 24 from Patent WO0200932.                      38   0.028  6
(EK407077) 1095505080418 Global-Ocean-Sampling_GS-31-01-01-1...    48   0.11   2
dna update 2009. 7. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BX548174_1381(BX548174|pid:none) Prochlorococcus marinus MED4 co...    35   3.2
(Q73BQ9) Indole-3-glycerol phosphate synthase (EC 4.1.1.48) (IGP...    35   3.2
AM285315_5(AM285315|pid:none) Spiroplasma citri GII3-3X chromoso...    35   4.2
T31826(T31826)hypothetical protein C17E7.3 - Caenorhabditis eleg...    34   5.4
EU016580_13(EU016580|pid:none) Uncultured marine microorganism H...    33   9.3
FM992693_183(FM992693|pid:none) Candida dubliniensis CD36 chromo...    33   9.3
AE000512_67(AE000512|pid:none) Thermotoga maritima MSB8, complet...    33   9.3
protein update 2009. 3.24
PSORT 

 psg: 0.65  gvh: 0.38  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VSI252 is cyt
5' end seq. ID VSI252F
5' end seq. 
>VSI252F.Seq
CTTGACAAAATAATAAATGATATTAAAAAAGAGGCTAACGACTCTGGTGTTACTTTAGCT
CCACTTTCTGTTCCAAAACCTAAACTAGAGGAACTCTCAGAGCAACAAAAAATTATACTT
GCCGAATACATTGCAGAAGTTGGTTTACAGAATATAACAGCGATCACTCTCTCAAAAAAA
TTGAATATTACCGTAGAAAAAGCTAAAAATTATATAAAAAATTCTAACAGACTTGGCAGA
ACAAATAATTTAAAAACTATTGGTATTCTTCAAGAAGAGGTTTCATCTATGGAGGCTAAA
TCTATGACTTGGTAAAGATTTAGGTATAGAACTTGGTTCATTATTTTTTCTAAAAAAAAT
AAAACACTAAAATTAAAATATGAAAATAAAATAAAATAAAGCTAATCATTTAATAGTGAT
TAACAAACCCTCATTGTACGTAAAATTAAAAAAAAAA----------
Length of 5' end seq. 457
3' end seq. ID VSI252Z
3' end seq. 
>VSI252Z.Seq
----------CTTGACAAAATAATAAATGATATTAAAAAAGAGGCTAACGACTCTGGTGT
TACTTTAGCTCCACTTTCTGTTCCAAAACCTAAACTAGAGGAACTCTCAGAGCAACAAAA
AATTATACTTGCCGAATACATTGCAGAAGTTGGTTTACAGAATATAACAGCGATCACTCT
CTCAAAAAAATTGAATATTACCGTAGAAAAAGCTAAAAATTATATAAAAAATTCTAACAG
ACTTGGCAGAACAAATAATTTAAAAACTATTGGTATTCTTCAAGAAGAGGTTTCATCTAT
GGAGGCTAAATCTATGACTTGGTAAAGATTTAGGTATAGAACTTGGTTCATTATTTTTTC
TAAAAAAAATAAAACACTAAAATTAAAATATGAAAATAAAATAAAATAAAGCTAATCTTT
AATAGNATTAACAAACCC
Length of 3' end seq. 428
Connected seq. ID VSI252P
Connected seq. 
>VSI252P.Seq
CTTGACAAAATAATAAATGATATTAAAAAAGAGGCTAACGACTCTGGTGTTACTTTAGCT
CCACTTTCTGTTCCAAAACCTAAACTAGAGGAACTCTCAGAGCAACAAAAAATTATACTT
GCCGAATACATTGCAGAAGTTGGTTTACAGAATATAACAGCGATCACTCTCTCAAAAAAA
TTGAATATTACCGTAGAAAAAGCTAAAAATTATATAAAAAATTCTAACAGACTTGGCAGA
ACAAATAATTTAAAAACTATTGGTATTCTTCAAGAAGAGGTTTCATCTATGGAGGCTAAA
TCTATGACTTGGTAAAGATTTAGGTATAGAACTTGGTTCATTATTTTTTCTAAAAAAAAT
AAAACACTAAAATTAAAATATGAAAATAAAATAAAATAAAGCTAATCATTTAATAGTGAT
TAACAAACCCTCATTGTACGTAAAATTAAAAAAAAAA----------CTTGACAAAATAA
TAAATGATATTAAAAAAGAGGCTAACGACTCTGGTGTTACTTTAGCTCCACTTTCTGTTC
CAAAACCTAAACTAGAGGAACTCTCAGAGCAACAAAAAATTATACTTGCCGAATACATTG
CAGAAGTTGGTTTACAGAATATAACAGCGATCACTCTCTCAAAAAAATTGAATATTACCG
TAGAAAAAGCTAAAAATTATATAAAAAATTCTAACAGACTTGGCAGAACAAATAATTTAA
AAACTATTGGTATTCTTCAAGAAGAGGTTTCATCTATGGAGGCTAAATCTATGACTTGGT
AAAGATTTAGGTATAGAACTTGGTTCATTATTTTTTCTAAAAAAAATAAAACACTAAAAT
TAAAATATGAAAATAAAATAAAATAAAGCTAATCTTTAATAGNATTAACAAACCC
Length of connected seq. 885
Full length Seq ID -
Full length Seq. -
Length of full length seq. -