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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI239
Library VS
(Link to library)
Clone ID VSI239
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16039-1
Original site URL
Representative seq. ID VSI239P
(Link to Original site)
Representative DNA sequence 
>VSI239 (VSI239Q) /CSM/VS/VSI2-B/VSI239Q.Seq.d/
AAAATAAACAAATAATATAAAAATAAGATAAAAAGATAGATAAATAAATTTAAACAATTA
ATAATAATTATTATTAAAAAATGATAATATTTAAAAATAATGAAATTCGATTCAATTCAA
TTTTAAAATCAATATTATTATTATTATTATTGTTTATCAATGGTAATAATTGTAAAACAG
CACAACAATTTTTAGATGAAAATTTTAATCATGCATTATTTAGAGAACATCAACTTGAAA
AAGAGTATAAAACCAAACAATTAGTAAAAGATGACCCAAATATGTATTCCGATTGGACAG
AGGTTCCAGCAGCAGCAGCAAACCAACCAAACCAACCAAATCAAGGACAATAATAAATTA
GTTTATTTAATTTAACTCTTATATAATAATACTAGGTCTCATTGTGAATTTATAAAACCT
TAAAATTAAATATAAAAAAAAAATAAAAATAAAAAAAATAAAAATAAAAATTCCCTTTCT
XXXXXXXXXXAAAATAANCAAATAATATAAAAATAAGATAAAAAGATAGATNAATAAATT
TAAACAATTAATAATAATTATTATTAAAAAATGATAATATTTAAAAATAATGAAATTCGA
TTCAATTCAATTTTAAAATCAATATTATTATTATTATTATTGTTTATCAATGGTAATAAT
TGTAAAACAGCACAACAATTTTTAGATGAAAATTTTAATCATGCATTATTTAGAGAACAT
CAACTTGAAAAAGAGTATAAAACCAAACAATTAGTAAAAGATGACCCAAATATGTATTCC
GATTGGACAGAGGTTCCAGCAGCAGCAGCAAACCAACCAAACCAACCAAATCAAGGACAA
TAATAAATTAGTTTATTTAATTTAACTCTTANATAATAATACTAGGTCTCATTGTGAATT
TATAAAACCTTAAAATTTAATATAAAAAAAAAATAAAAATAAAAAAAATAAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
k*tnnikir*kdr*inlnn***lllkndni*k**nsiqfnfkiniiiiiivyqw**l*ns
ttifr*kf*scii*rtst*krv*nqtiskr*pkyvfrldrgssssskptkptksrtiin*
fi*fnsyiiilglivnl*NLKIKYKKKIKIKKIKIKIPF---

---NXQII*k*dkkidx*i*tinnnyy*kmiifknneirfnsilksillllllfingnnc
ktaqqfldenfnhalfrehqlekeyktkqlvkddpnmysdwtevpaaaanqpnqpnqgq*
*islfnltlx**y*vsl*iyktlkfnikkk*k*kk*
Translated Amino Acid sequence (All Frames) 
Frame A:
kink*yknkikr*inkfkqliiiiikk**ylkimkfdsiqf*nqyyyyyyclsmviivkq
hnnf*mkilimhyleninlkksikpnn**kmtqicipigqrfqqqqqtnqtnqikdnnkl
vyli*llynntrshcefikp*n*i*kknknkknknknsls---

---kixk*yknkikr*xnkfkqliiiiikk**ylkimkfdsiqf*nqyyyyyyclsmvii
vkqhnnf*mkilimhyleninlkksikpnn**kmtqicipigqrfqqqqqtnqtnqikdn
nklvyli*llxnntrshcefikp*nli*kknknkknk

Frame B:
k*tnnikir*kdr*inlnn***lllkndni*k**nsiqfnfkiniiiiiivyqw**l*ns
ttifr*kf*scii*rtst*krv*nqtiskr*pkyvfrldrgssssskptkptksrtiin*
fi*fnsyiiilglivnl*NLKIKYKKKIKIKKIKIKIPF---

---k*xnnikir*kdrxinlnn***lllkndni*k**nsiqfnfkiniiiiiivyqw**l
*nsttifr*kf*scii*rtst*krv*nqtiskr*pkyvfrldrgssssskptkptksrti
in*fi*fnsxiiilglivnl*nlki*ykkkikikkik

Frame C:
nkqii*k*dkkidk*i*tinnnyy*kmiifknneirfnsilksillllllfingnnckta
qqfldenfnhalfrehqlekeyktkqlvkddpnmysdwtevpaaaanqpnqpnqgq**is
lfnltli**y*vsl*iyktlklnikkk*k*kk*k*kfpf---

---NXQII*k*dkkidx*i*tinnnyy*kmiifknneirfnsilksillllllfingnnc
ktaqqfldenfnhalfrehqlekeyktkqlvkddpnmysdwtevpaaaanqpnqpnqgq*
*islfnltlx**y*vsl*iyktlkfnikkk*k*kk*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI239 (VSI239Q) /CSM/VS/VSI2-B/VSI239Q.Seq.d/                        553   e-156
VSJ868 (VSJ868Q) /CSM/VS/VSJ8-C/VSJ868Q.Seq.d/                        105   1e-21
VSJ818 (VSJ818Q) /CSM/VS/VSJ8-A/VSJ818Q.Seq.d/                        105   1e-21
VSJ624 (VSJ624Q) /CSM/VS/VSJ6-A/VSJ624Q.Seq.d/                        105   1e-21
VSJ236 (VSJ236Q) /CSM/VS/VSJ2-B/VSJ236Q.Seq.d/                        105   1e-21
VSJ143 (VSJ143Q) /CSM/VS/VSJ1-B/VSJ143Q.Seq.d/                        105   1e-21
VSI854 (VSI854Q) /CSM/VS/VSI8-C/VSI854Q.Seq.d/                        105   1e-21
VSI805 (VSI805Q) /CSM/VS/VSI8-A/VSI805Q.Seq.d/                        105   1e-21
VSI619 (VSI619Q) /CSM/VS/VSI6-A/VSI619Q.Seq.d/                        105   1e-21
VSI293 (VSI293Q) /CSM/VS/VSI2-D/VSI293Q.Seq.d/                        105   1e-21
own update 2001.11.13
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268377) Dictyostelium discoideum vegetative cDNA clone:VS...   553   e-153  1
(AU268376) Dictyostelium discoideum vegetative cDNA clone:VS...   545   e-150  1
(X15380) Dictyostelium discoideum V4b gene.                       105   3e-18  1
(AU285043) Dictyostelium discoideum gamete cDNA clone:FCL-AB...   105   3e-18  1
(AU284569) Dictyostelium discoideum gamete cDNA clone:FC-BD0...   105   3e-18  1
(AU284418) Dictyostelium discoideum gamete cDNA clone:FC-AT2...   105   3e-18  1
(AU270604) Dictyostelium discoideum vegetative cDNA clone:VS...   105   3e-18  1
(AU270518) Dictyostelium discoideum vegetative cDNA clone:VS...   105   3e-18  1
(AU270214) Dictyostelium discoideum vegetative cDNA clone:VS...   105   3e-18  1
(AU269621) Dictyostelium discoideum vegetative cDNA clone:VS...   105   3e-18  1
dna update 2009. 7. 6
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3019103
Number of Hits to DB: 741,170,947
protein update 2009. 3.24
PSORT 

 psg: 0.52  gvh: -0.07  alm: 0.55  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.24  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VSI239 is nuc
5' end seq. ID VSI239F
5' end seq. 
>VSI239F.Seq
AAAATAAACAAATAATATAAAAATAAGATAAAAAGATAGATAAATAAATTTAAACAATTA
ATAATAATTATTATTAAAAAATGATAATATTTAAAAATAATGAAATTCGATTCAATTCAA
TTTTAAAATCAATATTATTATTATTATTATTGTTTATCAATGGTAATAATTGTAAAACAG
CACAACAATTTTTAGATGAAAATTTTAATCATGCATTATTTAGAGAACATCAACTTGAAA
AAGAGTATAAAACCAAACAATTAGTAAAAGATGACCCAAATATGTATTCCGATTGGACAG
AGGTTCCAGCAGCAGCAGCAAACCAACCAAACCAACCAAATCAAGGACAATAATAAATTA
GTTTATTTAATTTAACTCTTATATAATAATACTAGGTCTCATTGTGAATTTATAAAACCT
TAAAATTAAATATAAAAAAAAAATAAAAATAAAAAAAATAAAAATAAAAATTCCCTTTCT
----------
Length of 5' end seq. 480
3' end seq. ID VSI239Z
3' end seq. 
>VSI239Z.Seq
----------AAAATAANCAAATAATATAAAAATAAGATAAAAAGATAGATNAATAAATT
TAAACAATTAATAATAATTATTATTAAAAAATGATAATATTTAAAAATAATGAAATTCGA
TTCAATTCAATTTTAAAATCAATATTATTATTATTATTATTGTTTATCAATGGTAATAAT
TGTAAAACAGCACAACAATTTTTAGATGAAAATTTTAATCATGCATTATTTAGAGAACAT
CAACTTGAAAAAGAGTATAAAACCAAACAATTAGTAAAAGATGACCCAAATATGTATTCC
GATTGGACAGAGGTTCCAGCAGCAGCAGCAAACCAACCAAACCAACCAAATCAAGGACAA
TAATAAATTAGTTTATTTAATTTAACTCTTANATAATAATACTAGGTCTCATTGTGAATT
TATAAAACCTTAAAATTTAATATAAAAAAAAAATAAAAATAAAAAAAATAAAA
Length of 3' end seq. 463
Connected seq. ID VSI239P
Connected seq. 
>VSI239P.Seq
AAAATAAACAAATAATATAAAAATAAGATAAAAAGATAGATAAATAAATTTAAACAATTA
ATAATAATTATTATTAAAAAATGATAATATTTAAAAATAATGAAATTCGATTCAATTCAA
TTTTAAAATCAATATTATTATTATTATTATTGTTTATCAATGGTAATAATTGTAAAACAG
CACAACAATTTTTAGATGAAAATTTTAATCATGCATTATTTAGAGAACATCAACTTGAAA
AAGAGTATAAAACCAAACAATTAGTAAAAGATGACCCAAATATGTATTCCGATTGGACAG
AGGTTCCAGCAGCAGCAGCAAACCAACCAAACCAACCAAATCAAGGACAATAATAAATTA
GTTTATTTAATTTAACTCTTATATAATAATACTAGGTCTCATTGTGAATTTATAAAACCT
TAAAATTAAATATAAAAAAAAAATAAAAATAAAAAAAATAAAAATAAAAATTCCCTTTCT
----------AAAATAANCAAATAATATAAAAATAAGATAAAAAGATAGATNAATAAATT
TAAACAATTAATAATAATTATTATTAAAAAATGATAATATTTAAAAATAATGAAATTCGA
TTCAATTCAATTTTAAAATCAATATTATTATTATTATTATTGTTTATCAATGGTAATAAT
TGTAAAACAGCACAACAATTTTTAGATGAAAATTTTAATCATGCATTATTTAGAGAACAT
CAACTTGAAAAAGAGTATAAAACCAAACAATTAGTAAAAGATGACCCAAATATGTATTCC
GATTGGACAGAGGTTCCAGCAGCAGCAGCAAACCAACCAAACCAACCAAATCAAGGACAA
TAATAAATTAGTTTATTTAATTTAACTCTTANATAATAATACTAGGTCTCATTGTGAATT
TATAAAACCTTAAAATTTAATATAAAAAAAAAATAAAAATAAAAAAAATAAAA
Length of connected seq. 943
Full length Seq ID -
Full length Seq. -
Length of full length seq. -