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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI228
Library VS
(Link to library)
Clone ID VSI228
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15079-1
Original site URL
Representative seq. ID VSI228P
(Link to Original site)
Representative DNA sequence 
>VSI228 (VSI228Q) /CSM/VS/VSI2-B/VSI228Q.Seq.d/
AGATTCTTAAGAAAGACCGGTAGAAAGACTTTAGTCTCTCAAAATCCATACCATGCACTC
TTAGTCAAATTATACCGTTTCTTAGCCCGTAGAACCAACTCCAAGTTCAACAAGGTTGTC
TTAAAGAGATTATTACAAACTAAAACCAATAGACCACCAGTTTCAATCTCAAGAATTGCC
AATTTAATGAAGGACAGAAAGAGCGGTAAGATTGCTGTTTGTGTTGGTACCGTTGTTGAT
GATGAAAGATTATTAGACTGCCCAAAACTCACTGTTTGTGCATTAAGATTCACTGAAACT
GCTCGTTCAAGAATCGTCAAAGCTGGTGGTAAAGCATTATCTTTCGATCAATTCGCTTTA
GTTAGACCAAGAGGTAACAACACTTTCTTAATCAAAGGTGTTACCAAAGCTAGAGAAGTC
TACAAACATTTCGGTAGAGCTCCAGGTTTACCAGGTTCCCATGCTCGTCCTTATGTCCGT
TCAGAAGGTATTAAATTCCAAAGATCAAAGACCAGAAATCCATAAAGGCAAAAAGAAAAT
AAAGTCGCCCATTAAAAACAAAAGACTTTTTAAAATTAAAAAAAAAAXXXXXXXXXXCCA
AAAGGTGTTTACCAAAGCTAGAGAAGTCTACAAACATTTCGGTAGAGCTCCAGGTTTACC
AGGTTCCCATGCTCGTCCTTATGTCCGTTCAGAAGGTATTAAATTCCAAAGATCAAAGAC
CAGAAATCCATAAAGGCAAAAAGAAAATAAAGTCGCCCATTAAAAACAAAAGACTTTTAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
ds*erpverl*slkihtmhs*snytvs*pveptpsstrls*rdyyklkpidhqfqsqelp
i**rteravrllfvlvpllmmkdy*taqnslfvh*dslkllvqessklvvkhylsinsl*
ldqevttls*SKVLPKLEKSTNISVELQVYQVPMLVLMSVQKVLNSKDQRPEIHKGKKKI
KSPIKNKRLFKIKKK---

---KRCLPKLEKSTNISVELQVYQVPMLVLMSVQKVLNSKDQRPEIHKGKKKIKSPIKNK
RLL
Translated Amino Acid sequence (All Frames) 
Frame A:
rflrktgrktlvsqnpyhallvklyrflarrtnskfnkvvlkrllqtktnrppvsisria
nlmkdrksgkiavcvgtvvdderlldcpkltvcalrftetarsrivkaggkalsfdqfal
vrprgnntflikgvtkarevykhfgrapglpgsharpyvrsegikfqrsktrnp*rqken
kvah*kqktf*n*kk---

---pkgvyqs*rslqtfr*ssrftrfpcsslcpfrry*ipkikdqksikakrk*srplkt
kdf*

Frame B:
ds*erpverl*slkihtmhs*snytvs*pveptpsstrls*rdyyklkpidhqfqsqelp
i**rteravrllfvlvpllmmkdy*taqnslfvh*dslkllvqessklvvkhylsinsl*
ldqevttls*SKVLPKLEKSTNISVELQVYQVPMLVLMSVQKVLNSKDQRPEIHKGKKKI
KSPIKNKRLFKIKKK---

---qkvftkarevykhfgrapglpgsharpyvrsegikfqrsktrnp*rqkenkvah*kq
ktf

Frame C:
ilkkdr*kdfslsksipctlsqiipflsp*nqlqvqqgclkeiitn*nq*ttsfnlkncq
fnegqker*dcclcwyrc***kiirlpkthclcikih*ncsfknrqsww*siifrsirfs
*tkr*qhflnqrcyqs*rslqtfr*ssrftrfpcsslcpfrry*ipkikdqksikakrk*
srplktkdflklkkk---

---KRCLPKLEKSTNISVELQVYQVPMLVLMSVQKVLNSKDQRPEIHKGKKKIKSPIKNK
RLL
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ741 (VSJ741Q) /CSM/VS/VSJ7-B/VSJ741Q.Seq.d/                       1017   0.0
VSJ710 (VSJ710Q) /CSM/VS/VSJ7-A/VSJ710Q.Seq.d/                       1017   0.0
VSJ568 (VSJ568Q) /CSM/VS/VSJ5-C/VSJ568Q.Seq.d/                       1017   0.0
VSI415 (VSI415Q) /CSM/VS/VSI4-A/VSI415Q.Seq.d/                       1017   0.0
VSI379 (VSI379Q) /CSM/VS/VSI3-D/VSI379Q.Seq.d/                       1017   0.0
VSI228 (VSI228Q) /CSM/VS/VSI2-B/VSI228Q.Seq.d/                       1017   0.0
VSH755 (VSH755Q) /CSM/VS/VSH7-C/VSH755Q.Seq.d/                       1017   0.0
VSG426 (VSG426Q) /CSM/VS/VSG4-B/VSG426Q.Seq.d/                       1017   0.0
VSG175 (VSG175Q) /CSM/VS/VSG1-D/VSG175Q.Seq.d/                       1017   0.0
VSF604 (VSF604Q) /CSM/VS/VSF6-A/VSF604Q.Seq.d/                       1017   0.0
own update 2004. 8. 6
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU270400) Dictyostelium discoideum vegetative cDNA clone:VS...   987   0.0    1
(AU270352) Dictyostelium discoideum vegetative cDNA clone:VS...   987   0.0    1
(AU270129) Dictyostelium discoideum vegetative cDNA clone:VS...   987   0.0    1
(AU268658) Dictyostelium discoideum vegetative cDNA clone:VS...   987   0.0    1
(AU268604) Dictyostelium discoideum vegetative cDNA clone:VS...   987   0.0    1
(AU268356) Dictyostelium discoideum vegetative cDNA clone:VS...   987   0.0    1
(AU267922) Dictyostelium discoideum vegetative cDNA clone:VS...   987   0.0    1
(AU267499) Dictyostelium discoideum vegetative cDNA clone:VS...   987   0.0    1
(AU266461) Dictyostelium discoideum vegetative cDNA clone:VS...   987   0.0    1
(AU266085) Dictyostelium discoideum vegetative cDNA clone:VS...   987   0.0    1
dna update 2009. 7. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54VZ4) RecName: Full=60S ribosomal protein L18;                     347   3e-94
AY857431_1(AY857431|pid:none) Suberites domuncula L18 mRNA, comp...   206   6e-52
EU116900_1(EU116900|pid:none) Barentsia elongata putative riboso...   202   7e-51
(P35980) RecName: Full=60S ribosomal protein L18;  &AK009044_1(A...   202   9e-51
EU302542_1(EU302542|pid:none) Lineus viridis ribosomal protein r...   202   1e-50
CP000589_288(CP000589|pid:none) Ostreococcus lucimarinus CCE9901...   201   3e-50
(Q5E973) RecName: Full=60S ribosomal protein L18;  &BC104507_1(B...   200   5e-50
(P69090) RecName: Full=60S ribosomal protein L18;  &(P69091) Rec...   199   8e-50
BC082960_1(BC082960|pid:none) Xenopus tropicalis ribosomal prote...   199   1e-49
BC053777_1(BC053777|pid:none) Xenopus laevis major, mRNA (cDNA c...   198   1e-49
protein update 2009. 3.24
PSORT 

 psg: 0.73  gvh: 0.48  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.35  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 16.0 %: cytoplasmic
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VSI228 is nuc
5' end seq. ID VSI228F
5' end seq. 
>VSI228F.Seq
AGATTCTTAAGAAAGACCGGTAGAAAGACTTTAGTCTCTCAAAATCCATACCATGCACTC
TTAGTCAAATTATACCGTTTCTTAGCCCGTAGAACCAACTCCAAGTTCAACAAGGTTGTC
TTAAAGAGATTATTACAAACTAAAACCAATAGACCACCAGTTTCAATCTCAAGAATTGCC
AATTTAATGAAGGACAGAAAGAGCGGTAAGATTGCTGTTTGTGTTGGTACCGTTGTTGAT
GATGAAAGATTATTAGACTGCCCAAAACTCACTGTTTGTGCATTAAGATTCACTGAAACT
GCTCGTTCAAGAATCGTCAAAGCTGGTGGTAAAGCATTATCTTTCGATCAATTCGCTTTA
GTTAGACCAAGAGGTAACAACACTTTCTTAATCAAAGGTGTTACCAAAGCTAGAGAAGTC
TACAAACATTTCGGTAGAGCTCCAGGTTTACCAGGTTCCCATGCTCGTCCTTATGTCCGT
TCAGAAGGTATTAAATTCCAAAGATCAAAGACCAGAAATCCATAAAGGCAAAAAGAAAAT
AAAGTCGCCCATTAAAAACAAAAGACTTTTTAAAATTAAAAAAAAAA----------
Length of 5' end seq. 587
3' end seq. ID VSI228Z
3' end seq. 
>VSI228Z.Seq
----------CCAAAAGGTGTTTACCAAAGCTAGAGAAGTCTACAAACATTTCGGTAGAG
CTCCAGGTTTACCAGGTTCCCATGCTCGTCCTTATGTCCGTTCAGAAGGTATTAAATTCC
AAAGATCAAAGACCAGAAATCCATAAAGGCAAAAAGAAAATAAAGTCGCCCATTAAAAAC
AAAAGACTTTTAA
Length of 3' end seq. 183
Connected seq. ID VSI228P
Connected seq. 
>VSI228P.Seq
AGATTCTTAAGAAAGACCGGTAGAAAGACTTTAGTCTCTCAAAATCCATACCATGCACTC
TTAGTCAAATTATACCGTTTCTTAGCCCGTAGAACCAACTCCAAGTTCAACAAGGTTGTC
TTAAAGAGATTATTACAAACTAAAACCAATAGACCACCAGTTTCAATCTCAAGAATTGCC
AATTTAATGAAGGACAGAAAGAGCGGTAAGATTGCTGTTTGTGTTGGTACCGTTGTTGAT
GATGAAAGATTATTAGACTGCCCAAAACTCACTGTTTGTGCATTAAGATTCACTGAAACT
GCTCGTTCAAGAATCGTCAAAGCTGGTGGTAAAGCATTATCTTTCGATCAATTCGCTTTA
GTTAGACCAAGAGGTAACAACACTTTCTTAATCAAAGGTGTTACCAAAGCTAGAGAAGTC
TACAAACATTTCGGTAGAGCTCCAGGTTTACCAGGTTCCCATGCTCGTCCTTATGTCCGT
TCAGAAGGTATTAAATTCCAAAGATCAAAGACCAGAAATCCATAAAGGCAAAAAGAAAAT
AAAGTCGCCCATTAAAAACAAAAGACTTTTTAAAATTAAAAAAAAAA----------CCA
AAAGGTGTTTACCAAAGCTAGAGAAGTCTACAAACATTTCGGTAGAGCTCCAGGTTTACC
AGGTTCCCATGCTCGTCCTTATGTCCGTTCAGAAGGTATTAAATTCCAAAGATCAAAGAC
CAGAAATCCATAAAGGCAAAAAGAAAATAAAGTCGCCCATTAAAAACAAAAGACTTTTAA
Length of connected seq. 770
Full length Seq ID -
Full length Seq. -
Length of full length seq. -