NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI224
Library VS
(Link to library)
Clone ID VSI224
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U06930-1
Original site URL
Representative seq. ID VSI224P
(Link to Original site)
Representative DNA sequence 
>VSI224 (VSI224Q) /CSM/VS/VSI2-A/VSI224Q.Seq.d/
GTTTAAAGAAAAATAATAATAAAAATAATAATAATAATAATTTGAAAATGCAAGAAATAA
AATTACCAGTACCACCATTTAATAATATTGAAGATGCAATTAAAAAAGTTAGAATGGCAG
AAGATGCATGGAATTCAAGGAATGCAGAAACTGTTTCACTTGCATACAGTTTGGATACAA
AATGGAGAAATAGGAATGAATTTATTAATGGTAGAACACAAGTTGTTGAATTTTTAAAAA
AGAAATGGGAAAAAGAGGTTGAATATAGACTAATTAAAGAGTTGTGGACATTTCAAGCTG
GTAGTAATAGAATTGCCGTCAGATTTGCATATGAATGGAAAGATTTAAATGGTAATTGGT
TTAGATCATATGGAAATGAAAATTGGGAATTCAATGAATTTGGTCTAATGGTTCAAAGAC
ATGCATCAATAAATGATCTCTCAATTAGTGAATCACAAAGATTATTTCATTGGCCATGCG
GTAAAAGACCTGATGATCATCCAAGTTTATCAGATTTAGGTTTATAAAAAAATAAAATAA
AAAAATAAAAAAATAAAAGAATAAAAAAATAAAATAAAAAAAAAAXXXXXXXXXXCCACG
CGTCCCGGTTTAAAGAAAAATAATAATAAAAATAATAATAATAATAATTTGAAAATGCAA
GAAATAAAATTACCAGTACCACCATTTAATAATATTGAAGATGCAATTAAAAAAGTTAGA
ATGGCAGAAGATGCATGGAATTCAAGGAATGCAGAAACTGTTTCACTTGCATACAGTTTG
GATACAAAATGGAGAAATAGGAATGAATTTATTAATGGTAGAACACAAGTTGTTGAATTT
TTAAAAAAGAAATGGGAAAAAGAGGTTGAATATAGACTAATTAAAGAGTTGTGGACATTT
CAAGCTGGTAGTAATAGAATTGCCGTCAGATTTGCATATGAATGGAAAGATTTAAATGGT
AATTGGTTTAGATCATATGGAAATGAAAATTGGGAATTCAATGAATTTGGTCTAATGGTT
CAAAGACATGCATCAATAAATGATCTCTCAATTAGTGAATCACAAAGATTATTTCATTGG
CCATGCGGTAAAAGACCTGATGATCATCCAAGTTTATCAGATTTAGGTTTATAAAAAAAT
AAAATAAAAAAATAAAAAAAAAAAGANAAAAAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
v*rkiiikiiiiii*kckk*nyqyhhliilkmqlkklewqkmhgiqgmqklfhlhtvwiq
ngeigmnllmvehkllnf*krngkkrlnid*lkscghfklvvielpsdlhmngki*mvig
ldhmemkignsmnlv*wfkdmhq*misqlvnhkdyfighavkdlmiiqvyqi*vykkik*
knkkike*KNKIKKK---

---TRPGLKKNNNKNNNNNNLKMQEIKLPVPPFNNIEDAIKKVRMAEDAWNSRNAETVSL
AYSLDTKWRNRNEFINGRTQVVEFLKKKWEKEVEYRLIKELWTFQAGSNRIAVRFAYEWK
DLNGNWFRSYGNENWEFNEFGLMVQRHASINDLSISESQRLFHWPCGKRPDDHPSLSDLG
L*knkikk*kkkxkk
Translated Amino Acid sequence (All Frames) 
Frame A:
v*rkiiikiiiiii*kckk*nyqyhhliilkmqlkklewqkmhgiqgmqklfhlhtvwiq
ngeigmnllmvehkllnf*krngkkrlnid*lkscghfklvvielpsdlhmngki*mvig
ldhmemkignsmnlv*wfkdmhq*misqlvnhkdyfighavkdlmiiqvyqi*vykkik*
knkkike*KNKIKKK---

---prvpv*rkiiikiiiiii*kckk*nyqyhhliilkmqlkklewqkmhgiqgmqklfh
lhtvwiqngeigmnllmvehkllnf*krngkkrlnid*lkscghfklvvielpsdlhmng
ki*mvigldhmemkignsmnlv*wfkdmhq*misqlvnhkdyfighavkdlmiiqvyqi*
vykkik*knkkkkxkk

Frame B:
fkek***k*****fenarnkitstti**y*rcn*ks*ngrrcmefkecrncftciqfgyk
mek*e*iy*w*ntsc*ifkkemgkrg*i*tn*rvvdissw***ncrqici*merfkw*lv
*iiwk*klgiq*iwsngsktcink*sln**itkiislamr*kt**sskfirfrfikk*nk
kikk*knkkik*kk---

---hasrfkek***k*****fenarnkitstti**y*rcn*ks*ngrrcmefkecrncft
ciqfgykmek*e*iy*w*ntsc*ifkkemgkrg*i*tn*rvvdissw***ncrqici*me
rfkw*lv*iiwk*klgiq*iwsngsktcink*sln**itkiislamr*kt**sskfirfr
fikk*nkkikkkrxk

Frame C:
lkknnnknnnnnnlkmqeiklpvppfnniedaikkvrmaedawnsrnaetvslaysldtk
wrnrnefingrtqvveflkkkwekeveyrlikelwtfqagsnriavrfayewkdlngnwf
rsygnenwefnefglmvqrhasindlsisesqrlfhwpcgkrpddhpslsdlgl*knkik
k*knkrikk*nkkk---

---TRPGLKKNNNKNNNNNNLKMQEIKLPVPPFNNIEDAIKKVRMAEDAWNSRNAETVSL
AYSLDTKWRNRNEFINGRTQVVEFLKKKWEKEVEYRLIKELWTFQAGSNRIAVRFAYEWK
DLNGNWFRSYGNENWEFNEFGLMVQRHASINDLSISESQRLFHWPCGKRPDDHPSLSDLG
L*knkikk*kkkxkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ303 (VSJ303Q) /CSM/VS/VSJ3-A/VSJ303Q.Seq.d/                        916   0.0
VSI224 (VSI224Q) /CSM/VS/VSI2-A/VSI224Q.Seq.d/                        916   0.0
FC-AJ06 (FC-AJ06Q) /CSM/FC/FC-AJ/FC-AJ06Q.Seq.d/                       40   0.075
AFC859 (AFC859Q) /CSM/AF/AFC8-C/AFC859Q.Seq.d/                         40   0.075
SSM717 (SSM717Q) /CSM/SS/SSM7-A/SSM717Q.Seq.d/                         38   0.30
VSF739 (VSF739Q) /CSM/VS/VSF7-B/VSF739Q.Seq.d/                         36   1.2
VSF405 (VSF405Q) /CSM/VS/VSF4-A/VSF405Q.Seq.d/                         36   1.2
VSD453 (VSD453Q) /CSM/VS/VSD4-C/VSD453Q.Seq.d/                         36   1.2
VSB584 (VSB584Q) /CSM/VS/VSB5-D/VSB584Q.Seq.d/                         36   1.2
VHE268 (VHE268Q) /CSM/VH/VHE2-C/VHE268Q.Seq.d/                         36   1.2
own update 2004. 5.30
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268349) Dictyostelium discoideum vegetative cDNA clone:VS...   916   0.0    2
(AU269718) Dictyostelium discoideum vegetative cDNA clone:VS...   916   0.0    1
(AU269717) Dictyostelium discoideum vegetative cDNA clone:VS...   916   0.0    1
(AU268348) Dictyostelium discoideum vegetative cDNA clone:VS...   916   0.0    1
(FE268273) CAZO6646.fwd CAZO Naegleria gruberi Flagellate St...    42   0.002  2
(BJ374207) Dictyostelium discoideum cDNA clone:ddc6a04, 3' e...    36   0.094  3
(BJ374145) Dictyostelium discoideum cDNA clone:ddc6p17, 3' e...    36   0.095  3
(BJ437704) Dictyostelium discoideum cDNA clone:ddv35i04, 3' ...    36   0.098  3
(BJ439149) Dictyostelium discoideum cDNA clone:ddv39g18, 3' ...    36   0.11   3
(BJ346902) Dictyostelium discoideum cDNA clone:dda25a17, 3' ...    36   0.11   3
dna update 2009. 7. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000647_3992(CP000647|pid:none) Klebsiella pneumoniae subsp. pn...   233   1e-59
CP000125_1514(CP000125|pid:none) Burkholderia pseudomallei 1710b...   229   2e-58
CP000571_11(CP000571|pid:none) Burkholderia pseudomallei 668 chr...   229   2e-58
CP000085_11(CP000085|pid:none) Burkholderia thailandensis E264 c...   228   2e-58
CP000442_376(CP000442|pid:none) Burkholderia ambifaria AMMD chro...   228   3e-58
CP000094_2876(CP000094|pid:none) Pseudomonas fluorescens Pf0-1, ...   228   4e-58
BX571867_51(BX571867|pid:none) Photorhabdus luminescens subsp. l...   226   9e-58
CP000388_1119(CP000388|pid:none) Pseudoalteromonas atlantica T6c...   225   2e-57
AM039952_4431(AM039952|pid:none) Xanthomonas campestris pv. vesi...   225   2e-57
CP000091_1044(CP000091|pid:none) Ralstonia eutropha JMP134 chrom...   225   2e-57
protein update 2009. 3.24
PSORT 

 psg: 0.46  gvh: 0.16  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VSI224 is nuc
5' end seq. ID VSI224F
5' end seq. 
>VSI224F.Seq
GTTTAAAGAAAAATAATAATAAAAATAATAATAATAATAATTTGAAAATGCAAGAAATAA
AATTACCAGTACCACCATTTAATAATATTGAAGATGCAATTAAAAAAGTTAGAATGGCAG
AAGATGCATGGAATTCAAGGAATGCAGAAACTGTTTCACTTGCATACAGTTTGGATACAA
AATGGAGAAATAGGAATGAATTTATTAATGGTAGAACACAAGTTGTTGAATTTTTAAAAA
AGAAATGGGAAAAAGAGGTTGAATATAGACTAATTAAAGAGTTGTGGACATTTCAAGCTG
GTAGTAATAGAATTGCCGTCAGATTTGCATATGAATGGAAAGATTTAAATGGTAATTGGT
TTAGATCATATGGAAATGAAAATTGGGAATTCAATGAATTTGGTCTAATGGTTCAAAGAC
ATGCATCAATAAATGATCTCTCAATTAGTGAATCACAAAGATTATTTCATTGGCCATGCG
GTAAAAGACCTGATGATCATCCAAGTTTATCAGATTTAGGTTTATAAAAAAATAAAATAA
AAAAATAAAAAAATAAAAGAATAAAAAAATAAAATAAAAAAAAAA----------
Length of 5' end seq. 585
3' end seq. ID VSI224Z
3' end seq. 
>VSI224Z.Seq
----------CCACGCGTCCCGGTTTAAAGAAAAATAATAATAAAAATAATAATAATAAT
AATTTGAAAATGCAAGAAATAAAATTACCAGTACCACCATTTAATAATATTGAAGATGCA
ATTAAAAAAGTTAGAATGGCAGAAGATGCATGGAATTCAAGGAATGCAGAAACTGTTTCA
CTTGCATACAGTTTGGATACAAAATGGAGAAATAGGAATGAATTTATTAATGGTAGAACA
CAAGTTGTTGAATTTTTAAAAAAGAAATGGGAAAAAGAGGTTGAATATAGACTAATTAAA
GAGTTGTGGACATTTCAAGCTGGTAGTAATAGAATTGCCGTCAGATTTGCATATGAATGG
AAAGATTTAAATGGTAATTGGTTTAGATCATATGGAAATGAAAATTGGGAATTCAATGAA
TTTGGTCTAATGGTTCAAAGACATGCATCAATAAATGATCTCTCAATTAGTGAATCACAA
AGATTATTTCATTGGCCATGCGGTAAAAGACCTGATGATCATCCAAGTTTATCAGATTTA
GGTTTATAAAAAAATAAAATAAAAAAATAAAAAAAAAAAGANAAAAAAA
Length of 3' end seq. 579
Connected seq. ID VSI224P
Connected seq. 
>VSI224P.Seq
GTTTAAAGAAAAATAATAATAAAAATAATAATAATAATAATTTGAAAATGCAAGAAATAA
AATTACCAGTACCACCATTTAATAATATTGAAGATGCAATTAAAAAAGTTAGAATGGCAG
AAGATGCATGGAATTCAAGGAATGCAGAAACTGTTTCACTTGCATACAGTTTGGATACAA
AATGGAGAAATAGGAATGAATTTATTAATGGTAGAACACAAGTTGTTGAATTTTTAAAAA
AGAAATGGGAAAAAGAGGTTGAATATAGACTAATTAAAGAGTTGTGGACATTTCAAGCTG
GTAGTAATAGAATTGCCGTCAGATTTGCATATGAATGGAAAGATTTAAATGGTAATTGGT
TTAGATCATATGGAAATGAAAATTGGGAATTCAATGAATTTGGTCTAATGGTTCAAAGAC
ATGCATCAATAAATGATCTCTCAATTAGTGAATCACAAAGATTATTTCATTGGCCATGCG
GTAAAAGACCTGATGATCATCCAAGTTTATCAGATTTAGGTTTATAAAAAAATAAAATAA
AAAAATAAAAAAATAAAAGAATAAAAAAATAAAATAAAAAAAAAA----------CCACG
CGTCCCGGTTTAAAGAAAAATAATAATAAAAATAATAATAATAATAATTTGAAAATGCAA
GAAATAAAATTACCAGTACCACCATTTAATAATATTGAAGATGCAATTAAAAAAGTTAGA
ATGGCAGAAGATGCATGGAATTCAAGGAATGCAGAAACTGTTTCACTTGCATACAGTTTG
GATACAAAATGGAGAAATAGGAATGAATTTATTAATGGTAGAACACAAGTTGTTGAATTT
TTAAAAAAGAAATGGGAAAAAGAGGTTGAATATAGACTAATTAAAGAGTTGTGGACATTT
CAAGCTGGTAGTAATAGAATTGCCGTCAGATTTGCATATGAATGGAAAGATTTAAATGGT
AATTGGTTTAGATCATATGGAAATGAAAATTGGGAATTCAATGAATTTGGTCTAATGGTT
CAAAGACATGCATCAATAAATGATCTCTCAATTAGTGAATCACAAAGATTATTTCATTGG
CCATGCGGTAAAAGACCTGATGATCATCCAAGTTTATCAGATTTAGGTTTATAAAAAAAT
AAAATAAAAAAATAAAAAAAAAAAGANAAAAAAA
Length of connected seq. 1164
Full length Seq ID -
Full length Seq. -
Length of full length seq. -