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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSI223
Library VS
(Link to library)
Clone ID VSI223
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U06929-1
Original site URL
Representative seq. ID VSI223E
(Link to Original site)
Representative DNA sequence 
>VSI223 (VSI223Q) /CSM/VS/VSI2-A/VSI223Q.Seq.d/
AGTTCATTCATTTAGTCGTATGATAGTATCACCATTTATAAATCCAAAATTCACTTATTT
CGGTAGATATGGTTGGTTAAAATTTGATATCCCAATCAACAAGAAATCAAATCGTATAGT
TTTAAAACCACCATATGTTCTTTCAATTCGGTAAGAATACATTCTTATCATCATTAGAAA
TCACTTCACGTTTAGGTGATATGTCACGTTATTTCTCTGATCATTATTTTAGTAATAAAG
TTTTAAATAGTGATAATGAATCAAATTCATTTAGTAGAAGTATGAGTGTAAATAATAATT
TAAATGAATCATTATCATCATCGTCATCACCATCAGCTATTAGTAATAATAGTAATAATA
GTAATAATAATGATGATAATAGACCACCAATATTAGGATTACCATTTTTATTTGATTATA
AAAATAGAATTACCAGAGGATCAAGATTCTTTGAAACTATTCATTATAAGATCGTTCATG
TAACTTCTGCAACTGAATTTGGTATTAGAAGAATTAGTAAATTGTATGGTACAAAATGGC
AATTAGAAATTGGTTTAAAACATCAAATAACTCAAAGTGGTGCACTTCAATGTTTATTCA
CTCATACAATTGGCCAAACTACAATTTTTGGTTTAAGCTTTGGTTTTGATATGTAATTTT
GTAATAATTAATTACACATCAAAAATTATATTCAAATAAATTAAATTAAATTAAATTAAA
TAAATAA
sequence update 2001. 3.25
Translated Amino Acid sequence 
fihlvv**yhhl*iqnslisvdmvg*nlisqstrnqiv*f*NHHMFFQFGKNTFLSSLEI
TSRLGDMSRYFSDHYFSNKVLNSDNESNSFSRSMSVNNNLNESLSSSSSPSAISNNSNNS
NNNDDNRPPILGLPFLFDYKNRITRGSRFFETIHYKIVHVTSATEFGIRRISKLYGTKWQ
LEIGLKHQITQSGALQCLFTHTIGQTTIFGLSFGFDM*fcnn*lhiknyiqin*ikln*i
n
Translated Amino Acid sequence (All Frames) 
Frame A:
ssfi*sydsitiykskihlfr*iwlvki*ypnqqeiksysfktticsfnsvrihsyhh*k
slhv*vichvisliiilvikf*ivimnqihlvev*v*iii*mnhyhhrhhhqllviivii
viimmiidhqy*dyhfyliikielpedqdslklfiirsfm*llqlnlvleelvncmvqng
n*klv*nik*lkvvhfnvysliqlaklqflv*alvlicnfviinytskiifk*ikln*ik
*i


Frame B:
vhsfsrmivspfinpkftyfgrygwlkfdipinkksnrivlkppyvlsir*eyiliiirn
hftfr*yvtlfl*slf***sfk****ikfi**kyeck**fk*iiiiivitisy*******
*******ttniritifi*l*k*nyqrikil*nysl*drscnfcn*iwy*kn**ivwykma
irnwfktsnnskwctsmfihsynwpnynfwfklwf*yvil**lithqklysnkln*ikln
k*


Frame C:
fihlvv**yhhl*iqnslisvdmvg*nlisqstrnqiv*f*NHHMFFQFGKNTFLSSLEI
TSRLGDMSRYFSDHYFSNKVLNSDNESNSFSRSMSVNNNLNESLSSSSSPSAISNNSNNS
NNNDDNRPPILGLPFLFDYKNRITRGSRFFETIHYKIVHVTSATEFGIRRISKLYGTKWQ
LEIGLKHQITQSGALQCLFTHTIGQTTIFGLSFGFDM*fcnn*lhiknyiqin*ikln*i
n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSI223 (VSI223Q) /CSM/VS/VSI2-A/VSI223Q.Seq.d/                        674   0.0
VHM362 (VHM362Q) /CSM/VH/VHM3-C/VHM362Q.Seq.d/                         54   3e-06
VHK407 (VHK407Q) /CSM/VH/VHK4-A/VHK407Q.Seq.d/                         54   3e-06
VHC794 (VHC794Q) /CSM/VH/VHC7-D/VHC794Q.Seq.d/                         54   3e-06
VHC364 (VHC364Q) /CSM/VH/VHC3-C/VHC364Q.Seq.d/                         54   3e-06
VHC858 (VHC858Q) /CSM/VH/VHC8-C/VHC858Q.Seq.d/                         48   2e-04
AHO191 (AHO191Q) /CSM/AH/AHO1-D/AHO191Q.Seq.d/                         44   0.003
AFF757 (AFF757Q) /CSM/AF/AFF7-C/AFF757Q.Seq.d/                         44   0.003
VFO436 (VFO436Q) /CSM/VF/VFO4-B/VFO436Q.Seq.d/                         42   0.012
VFN870 (VFN870Q) /CSM/VF/VFN8-C/VFN870Q.Seq.d/                         42   0.012
own update 2004. 6. 2
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU268346) Dictyostelium discoideum vegetative cDNA clone:VS...   426   0.0    3
(AU268347) Dictyostelium discoideum vegetative cDNA clone:VS...   539   0.0    2
(AZ167278) SP_0098_A1_A10_SP6E Strongylocentrotus purpuratus...    48   3e-04  2
(AC181342) Strongylocentrotus purpuratus clone R3-40D8, WORK...    50   4e-04  2
(AC100956) Mus musculus chromosome 7, clone RP23-74B10, comp...    56   0.002  1
(AC140360) Mus musculus BAC clone RP24-241G4 from chromosome...    54   0.008  1
(DD307412) NUCLEIC ACID AND POLYPEPTIDE SEQUENCES FROM LAWSO...    54   0.008  1
(DD307082) NUCLEIC ACID AND POLYPEPTIDE SEQUENCES FROM LAWSO...    54   0.008  1
(DD300861) NUCLEIC ACID AND POLYPEPTIDE SEQUENCES FROM LAWSO...    54   0.008  1
(Z37981) D.discoideum gene for protein kinase.                     54   0.008  1
dna update 2009. 7. 5
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3019103
Number of Hits to DB: 844,226,636
protein update 2009. 3.24
PSORT 

 psg: 0.74  gvh: 0.38  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for VSI223 is nuc
5' end seq. ID VSI223F
5' end seq. 
>VSI223F.Seq
AGTTCATTCATTTAGTCGTATGATAGTATCACCATTTATAAATCCAAAATTCACTTATTT
CGGTAGATATGGTTGGTTAAAATTTGATATCCCAATCAACAAGAAATCAAATCGTATAGT
TTTAAAACACCATATGTTCTTTCAATTCGGTAAGAATACATTCTTATCATCATTAGAAAT
CACTTCACGTTTAGGTGATATGTCACGTTATTTCTCTGATCATTATTTTAGTAATAAAGT
TTTAAATAGTGATAATGAATCAAATTCATTTAGTAGAAGTATGAGTGTAAATAATAATTT
AAATGAATCATTATCATCATCGTCATCACCATCAGCTATTAGTAATAATAGTAATAATAG
TAATAATAATGATGATAATAGACCACCAATATTAGGATTACCATTTTTATTTGATTATAA
AAATAGAATTACCAGAGGATCAAGATTCTTTGAAACTATTCATTATAAGATCGTTCATGT
AACTTCTGCAACTGAATTTGGTATTAGAAGAATTAGTAAATTGTATGGTACAAAATGGCA
ATTAGAAATTGGTTTAAAACATCAAATAACT----------
Length of 5' end seq. 571
3' end seq. ID VSI223Z
3' end seq. 
>VSI223Z.Seq
----------GAAATCAAATCGTATAGTTTTAAAACCACCATATGTTCTTTCAATTCGGT
AAGAATACATTCTTATCATCATTAGAAATCACTTCACGTTTAGGTGATATGTCACGTTAT
TTCTCTGATCATTATTTTAGTAATAAAGTTTTAAATAGTGATAATGAATCAAATTCATTT
AGTAGAAGTATGAGTGTAAATAATAATTTAAATGAATCATTATCATCATCGTCATCACCA
TCAGCTATTAGTAATAATAGTAATAATAGTAATAATAATGATGATAATAGACCACCAATA
TTAGGATTACCATTTTTATTTGATTATAAAAATAGAATTACCAGAGGATCAAGATTCTTT
GAAACTATTCATTATAAGATCGTTCATGTAACTTCTGCAACTGAATTTGGTATTAGAAGA
ATTAGTAAATTGTATGGTACAAAATGGCAATTAGAAATTGGTTTAAAACATCAAATAACT
CAAAGTGGTGCACTTCAATGTTTATTCACTCATACAATTGGCCAAACTACAATTTTTGGT
TTAAGCTTTGGTTTTGATATGTAATTTTGTAATAATTAATTACACATCAAAAATTATATT
CAAATAAATTAAATTAAATTAAATTAAATAAATAA
Length of 3' end seq. 625
Connected seq. ID VSI223P
Connected seq. 
>VSI223P.Seq
AGTTCATTCATTTAGTCGTATGATAGTATCACCATTTATAAATCCAAAATTCACTTATTT
CGGTAGATATGGTTGGTTAAAATTTGATATCCCAATCAACAAGAAATCAAATCGTATAGT
TTTAAAACACCATATGTTCTTTCAATTCGGTAAGAATACATTCTTATCATCATTAGAAAT
CACTTCACGTTTAGGTGATATGTCACGTTATTTCTCTGATCATTATTTTAGTAATAAAGT
TTTAAATAGTGATAATGAATCAAATTCATTTAGTAGAAGTATGAGTGTAAATAATAATTT
AAATGAATCATTATCATCATCGTCATCACCATCAGCTATTAGTAATAATAGTAATAATAG
TAATAATAATGATGATAATAGACCACCAATATTAGGATTACCATTTTTATTTGATTATAA
AAATAGAATTACCAGAGGATCAAGATTCTTTGAAACTATTCATTATAAGATCGTTCATGT
AACTTCTGCAACTGAATTTGGTATTAGAAGAATTAGTAAATTGTATGGTACAAAATGGCA
ATTAGAAATTGGTTTAAAACATCAAATAACT----------GAAATCAAATCGTATAGTT
TTAAAACCACCATATGTTCTTTCAATTCGGTAAGAATACATTCTTATCATCATTAGAAAT
CACTTCACGTTTAGGTGATATGTCACGTTATTTCTCTGATCATTATTTTAGTAATAAAGT
TTTAAATAGTGATAATGAATCAAATTCATTTAGTAGAAGTATGAGTGTAAATAATAATTT
AAATGAATCATTATCATCATCGTCATCACCATCAGCTATTAGTAATAATAGTAATAATAG
TAATAATAATGATGATAATAGACCACCAATATTAGGATTACCATTTTTATTTGATTATAA
AAATAGAATTACCAGAGGATCAAGATTCTTTGAAACTATTCATTATAAGATCGTTCATGT
AACTTCTGCAACTGAATTTGGTATTAGAAGAATTAGTAAATTGTATGGTACAAAATGGCA
ATTAGAAATTGGTTTAAAACATCAAATAACTCAAAGTGGTGCACTTCAATGTTTATTCAC
TCATACAATTGGCCAAACTACAATTTTTGGTTTAAGCTTTGGTTTTGATATGTAATTTTG
TAATAATTAATTACACATCAAAAATTATATTCAAATAAATTAAATTAAATTAAATTAAAT
AAATAA
Length of connected seq. 1196
Full length Seq ID VSI223E
Full length Seq. 
>VSI223E.Seq
AGTTCATTCATTTAGTCGTATGATAGTATCACCATTTATAAATCCAAAATTCACTTATTT
CGGTAGATATGGTTGGTTAAAATTTGATATCCCAATCAACAAGAAATCAAATCGTATAGT
TTTAAAACCACCATATGTTCTTTCAATTCGGTAAGAATACATTCTTATCATCATTAGAAA
TCACTTCACGTTTAGGTGATATGTCACGTTATTTCTCTGATCATTATTTTAGTAATAAAG
TTTTAAATAGTGATAATGAATCAAATTCATTTAGTAGAAGTATGAGTGTAAATAATAATT
TAAATGAATCATTATCATCATCGTCATCACCATCAGCTATTAGTAATAATAGTAATAATA
GTAATAATAATGATGATAATAGACCACCAATATTAGGATTACCATTTTTATTTGATTATA
AAAATAGAATTACCAGAGGATCAAGATTCTTTGAAACTATTCATTATAAGATCGTTCATG
TAACTTCTGCAACTGAATTTGGTATTAGAAGAATTAGTAAATTGTATGGTACAAAATGGC
AATTAGAAATTGGTTTAAAACATCAAATAACTCAAAGTGGTGCACTTCAATGTTTATTCA
CTCATACAATTGGCCAAACTACAATTTTTGGTTTAAGCTTTGGTTTTGATATGTAATTTT
GTAATAATTAATTACACATCAAAAATTATATTCAAATAAATTAAATTAAATTAAATTAAA
TAAATAA
Length of full length seq. 727